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The
intermediate image is further magnified by the eyepiece. The microscopist is able to observe a
greatly enlarged virtual image of the specimen by peering through the eyepieces.
The total magnification of a microscope is determined by multiplying the individual
magnifications of the objective and eyepiece. This section discusses the basic concepts associated with
optical microscopy, including objectives, eyepieces, condensers, stages, magnification, numerical
aperture, optical aberrations, and a variety of related topics.
3.3 Introduction to Microscopy
Microscopes are instruments designed to produce magnified visual or photographic images of
objects too small to be seen with the naked eye. The microscope must accomplish three tasks:
produce a magnified image of the specimen, separate the details in the image, and render the details
visible to the human eye or camera. This group of instruments includes not only multiple-lens
(compound microscopes) designs with objectives and condensers, but also very simple single lens
instruments that are often hand-held, such as a loupe or magnifying glass.
Modern compound microscopes are designed to provide a magnified two-dimensional image
that can be focused axially in successive focal planes, thus enabling a thorough examination of
specimen fine structural detail in both two and three dimensions.
Most microscopes provide a translation mechanism attached to the stage that allows the
microscopist to accurately position, orient, and focus the specimen to optimize visualization and
recording of images. The intensity of illumination and orientation of light pathways throughout the
microscope can be controlled with strategically placed diaphragms, mirrors, prisms, beam splitters,
and other optical elements to achieve the desired degree of brightness and contrast in the specimen.
Presented in Fig.3.1 is a typical microscope equipped with a trinocular head and 35-millimeter
camera system for recording photomicrographs. Illumination is provided by a tungsten-halogen lamp
positioned in the lamp house, which emits light that first passes through a collector lens and then into
an optical pathway in the microscope base.
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Also stationed in the microscope base is a series of filters that condition the light emitted by
the incandescent lamp before it is reflected by a mirror and passed through the field diaphragm and
into the substage condenser. The condenser forms a cone of illumination that bathes the specimen,
located on the microscope stage, and subsequently enters the objective.
Light leaving the objective is diverted by a beam splitter/prism combination either into the
eyepieces to form a virtual image, or straight through to the projection lens mounted in the trinocular
extension tube, where it can then form a latent image on film housed in the camera system.
The optical components contained within modern microscopes are mounted on a stable,
ergonomically designed base that allows rapid exchange, precision centering, and careful alignment
between those assemblies that are optically interdependent.
Together, the optical and mechanical components of the microscope, including the mounted
specimen on a glass micro slide and cover slip, form an optical train with a central axis that traverses
the microscope base and stand.
The intermediate image plane is usually located about 10 millimeters below the top of the
microscope body tube at a specific location within the fixed internal diaphragm of the eyepiece
(Fig.3.2). The distance between the back focal plane of the objective and the intermediate image is
termed the optical tube length. Note that this value is different from the mechanical tube length of a
microscope, which is the distance between the nosepiece (where the objective is mounted) to the top
edge of the observation tubes where the eyepieces (oculars) are inserted.
The eyepiece or ocular, which fits into the
body tube at the upper end, is the farthest optical
component from the specimen. In modern
microscopes, the eyepiece is held into place by a
shoulder on the top of the microscope observation
tube, which keeps it from falling into the tube.
The placement of the eyepiece is such that its eye
(upper) lens further magnifies the real image
projected by the objective. The eye of the
observer sees this secondarily magnified image as
if it were at a distance of 25 centimeters from the
eye; hence this virtual image appears as if it
were near the base of the microscope. The
distance from the top of the microscope
observation tube to the shoulder of the objective
(where it fits into the nosepiece) is usually 160
mm in a finite tube length system.
The eyepiece serves to further magnify the real image projected by the objective;
The eyepiece can be fitted with scales, markers or crosshairs (often referred to as graticules,
reticules or reticles) in such a way that the image of these inserts can be superimposed on the
image of the specimen.
The factor that determines the amount of image magnification is the objective magnifying
power, which is predetermined during construction of the objective optical elements. Objectives
typically have magnifying powers that range from 1:1 (1X) to 100:1 (100X), with the most common
powers being 4X (or 5X), 10X, 20X, 40X (or 50X), and 100X. An important feature of microscope
objectives is their very short focal lengths that allow increased magnification at a given distance when
compared to an ordinary hand lens. The primary reason that microscopes are so efficient at
magnification is the two-stage enlargement that is achieved over such a short optical path, due to the
short focal lengths of the optical components.
Eyepieces, like objectives, are classified in terms of their ability to magnify the intermediate
image. Their magnification factors vary between 5X and 30X with the most commonly used
eyepieces having a value of 10X-15X. Total visual magnification of the microscope is derived by
multiplying the magnification values of the objective and the eyepiece. For instance, using a 5X
objective with a 10X eyepiece yields a total visual magnification of 50X and likewise, at the top end
of the scale, using a 100X objective with a 30X eyepiece gives a visual magnification of 3000X.
Total magnification is also dependent upon the tube length of the microscope. Most standard
fixed tube length microscopes have a tube length of 160, 170, 200, or 210 millimeters. Many
industrial microscopes, designed for use in the semiconductor industry, have a tube length of 210
millimeters. The objectives and eyepieces of these microscopes have optical properties designed for a
specific tube length, and using an objective or eyepiece in a microscope of different tube length will
lead to changes in the magnification factor (and may also lead to an increase in optical aberration lens
errors). Infinity-corrected microscopes also have eyepieces and objectives that are optically-tuned to
the design of the microscope, and these should not be interchanged between microscopes with different
infinity tube lengths.
In Fig.3.3 is shown a Tool Maker Microscope. The Tool Makers Microscope (TMM) essentially
consists of the cast base, the main lighting unit, the upright with carrying arm and the sighting
microscope. The rigid cast base is resting on three foots screws by means of which the equipment
can be leveled with reference to the build-in box level.
The base carries the co-ordinate measuring table, consists of two measuring slides; one each
for directions X and Y and a rotary circular table provided with the glass plate. The slides are
running on precision balls in hardened guide ways warranting reliable travel.
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Two micrometer screws each of them measuring range of 0 to 25 mm permit the measuring
table to be displaced in the directions X and Y. The range of movements of the carriage can be
widened up to 150 mm in the X direction and up to 50 mm in the Y direction with the use of gage
blocks.
The rotary table has been provided with 360 degrees graduation and with a three minute
vernier. The rotary motion is initiated by activation of knurled knob and locked with star handle screw.
Slots in the rotary table serve for fastening different accessories and completing elements.
The sighting microscope has been fastened with a carrier arm to column. The carrier arm can be
adjusted in height by means of a rack and locked with star handle screw.
Thread measuring according to the shadow image permits the column to be tilted in X
direction to either side about an axis on centre plane level. The corresponding swivel can be adjusted
with a knurled knob with a graduation cellar.
The main lighting unit has been arranged in the rear of the cast base and equipped with
projection lamp where rays are directed via stationary mounted mirror through table glass plate into
the sighting microscope.
Measuring principle: The work piece to be checked is arranged in the path of the rays of the
lighting equipment. It produces a shadow image, which is viewed with the microscope eyepiece
having either a suitable mark for aiming at the next points of the objects or in case of often occurring
profiles. e.g. Threads or rounding standard line pattern for comparison with the shadow image of
the text object is projected to a ground glass screen. The text object is shifted or turned on the
measuring in addition to the comparison of shapes.
The addition to this method (shadow image method), measuring operations are also possible by
use of the axial reaction method, which can be recommended especially for thread measuring. This
involves approached measuring knife edges and measurement in axial section of thread according to
definition. This method permits higher precision than shadow image method for special measuring
operations.
Applications: The large tool makers microscope is suitable for the following fields of
applications:
- Length measurement in cartesian and polar co-ordinates;
- Angle measurements of tools; threading tools punches and gauges, templates etc.
- Thread measurements i.e., profile major and minor diameters, height of lead, thread angle,
profile position with respect to the thread axis and the shape of thread. (rounding, flattering,
straightness of flanks)
- Single point lathe tool angle measurements.
Procedure of measurement with TMM. Place the tool bit on the glass stage so as to obtain a
clear image on which angular measurements are done (Fig.3.4). Focus the microscope to get a real
image super imposed on the graticule pattern of the eyepiece. Tilt the graticule pattern so as to align
the shank edge with the reference hair line. Read microscope angle scale. Tilt the angle so as to
bring the cutting edge of the tool to align with the reference hairline. If necessary X, Y movements
may be made to retain the edge in the field of view. A typical field of vision before and after adjustment
is shown in Fig. 3.5
Fig.3.4 Orientation of face and flank surfaces with respect to machine reference system.
3. Projection lens system which magnifies and transmits the object contour or image
resulting from the collimated parallel light rays; and
4. Viewing screen, on which the projected contour or image of the object appears and is
displayed for inspection.
The main elements schematic arrangement is
shown in Fig.3.6, where the light travel path is
indicated by arrows. Light rays originating in the
light source hit the object, whose physical body
creates a shadow bounded by the actual contour
of the object when viewed in the direction of the
light rays. This shadow is then magnified by the
lens system and projected on the viewing screen.
Fig.3.6 Schematic view of the optical system in typical
projecting lenses.
When choosing the most favorable magnification for the viewing of the object, two opposing
aspects must be brought into proper balance:
a. The higher the magnification, the better definition may be obtained of the intricate details
of the object;
b. The lower the magnification, the larger will be the field diameter, that is, that area of the
object which can be projected onto the screen.
This relationship may be expressed by the following simple formula:
Field diameter
10
Screen diameter
Lens magnification
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The most common application of the optical projector is based on projecting the shadow
image of the object on the viewing screen. The shadow, or silhouette, represents the contour of the
object in that plane which is brought to coincide with the focal plane of the optical system. For round
objects, this should be identical with the diametral plane, where the projection of the silhouette
faithfully represents the cross sectional contour of the part. For flat pieces of essentially uniform
thickness the projected image is, for most practical purposes, also a true replica of the object contour.
This claim is particularly valid for relatively thin objects; however, in the case of flat parts with greater
thickness the projected shadow could suffer in sharpness of the contour. Regular bodies of revolution,
or substantially round objects whose surface contour is either continuous or repetitive, e.g., the teeth of
thread cutting taps, gears, milling cutters etc., are primarily suited for the inspection of the
geometric characteristics by means of a projected shadow.
a)
b)
Fig.3.7 a) Digital Profile Projector; b) Digital comparator.
This Digital Profile Projector (Fig.3.7a) can effectively test several of work-pieces with
complex shapes, such as templates punching pieces, cams, gears, threads by profile comparative and
coordinates measuring ways.
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Resolution: 0.001mm
Digital comparator (Fig.3.7b) can test effectively profile, section and surface shapes of
various complicated work-pieces, such as templates, punching pieces, cams, gears, forming cutters,
mercerizes etc. it is widely used in workshops and measuring stations of those industries: machinery,
instrument & meter, watch-making, mould and electron etc.
Characteristics:
Resolution: 0.001mm
Magnification
10X (Standard)
20X (Optional)
30X (Optional)
100X (Optional)
View-field on object
35mm
17.5mm
7mm
3.5mm
Working distance
88.376mm
81.375mm
54mm
44.9mm
Magnification error
0.08%
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