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Bacteria are diverse in shape and size, adaptation to environment, and metabolic
processes
Descriptive term
Adaptive feature
Psychrophile
Mesophile
Thermophile
Thermoduric
Halophile
Salt-tolerant
Acidophile
Acid-tolerant
Aerobe
Obligate anaerobe
Autotroph
Heterotroph
Cell wall
-
Gram stain
Steps:
1.
2.
3.
4.
Gram-positive wall
-
Cytoplasmic membrane
-
Cytoplasm
Inclusion granules
together
Biofilm formation begins with attaching to surface and form a cement cells to the
surface to protect the bacteria from hazardous materials
Bacterial sporulation
- Not part of the reproductive cycle but enables the bacteria to survive in
-
Bacterial toxin
- Normal flora bacteria that are not pathogenic but can be opportunistic
- Pathogenic bacteria that is not a normal flora and cause diseases
- Toxins products of bacteria that produce immediate host cell damage
Endotoxin
Exotoxin
A-B toxin contains B subunit that binds to host cell receptor and bound to the A
subunit that mediates enzyme activity for toxicity
Cytolytic toxins does not have A and B subunit but work enzymatically to
attack cell that causes cell lysis
Traditional cultivation
-
1. Fastidious
- Complex or extensive nutritional requirements
- Culture Media should be supplemented
2. Non fastidious
- Basic nutritional requirements
Bacterial growth
Growth curve
a. Lag phase little or no cell division; intense metabolic activity
b. Log phase - AKA Exponential growth phase; cell begins to divide; active
cellular reproduction with constant minimum generation time; cells are at their
most active state
c. Stationary phase - -growth rate slows down (# of new cells = # of microbial
deaths = population stabilizes) period of equilibrium
d. Death phase - logarithmic decline; number of deaths exceeds the number of
new cells formed