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UREA

BIOLABO REAGENTS

www.biolabo,fr

Colorimetric Method

MA!UFACTURERI
BIOLABO SA"

Reagent for quantitative determination of urea


in human serum and plasma, or urines.

02160, Malzv, France

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Rlrx125mL R21x1.25mL R31x31mL R41x10mt


R31xl25mL R41x10mL

880321 Rt1x500mL R21x5mL


TECHI{ICAL SUPPORT AI{D OROERS

Tel : (33)

03 23 25 15 50

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IIIMUXWffiEUN
REAGEI'ITS PREPARATION
More than 90% of urea is excreted throuqh the kidneys in u.ines.
Measurement of the plasma or Serum urea concentration is widely
reqarded as a test of renal function. However, a number of nonrenal

tfE circulating urea concentration: Urea


increased level occurs when proteins cataboiism is accelerated, burns,

faators atso influence

stress, myocardial infilction... lJrea is de.reased. in acute liver


destruction and is accompagnied with increased ammonium level
Urea level]s qenerally studied in conjunction with creatinine level
ratio) to refine post-renal or pre-renal diagnosis

(urea/creatinine

Add contents of vial R2 (Urease) into vial R1 (Salicylate). I.4ix gently by


inversion.

Alkaline (vial R3l:


Procedure

n1 and nZ (man!al): Dilute (1 + 3) with demineralised

water
Procedure n3 (manual or automatic): Ready to use

stslgA4Ljyiil&U I transferthe

requested quantity, recap and store at

2-8t]PRINCIPLE

{4)

Enzymatic and colorimetric method based on the specific action of


urease which hydrolyses urea ih ammonium ions and carbon dioxide
Ammonium ions then fom with chloride and salicylate a blue-green
complexe. This coloration, proportional to urea concentration in the

STABILITY AND STORAGE


StoE at 2-8f, well Ecap in thelriginal Yialand asayfrcm light'

Unopened, reagents are stable until expiry date stated on the label
when stored and used as described:in the insert.
Once reconstituted, working reagent (R1+R2) is stable fq tr msFth
when frce from contaminaiion.
. Once opened, Base (vial R3) diluted % is stable for 3 months when
free from contamination.
. once opened, the contents of vial R4 is stable for at least3 months
when free hom contamination.
Discard any reagent lf cloudy or if absorbance of blank against water

speclmen, is measured at 600 nm.

REAGEI{TS

col,lPosmoil

|TI:r]l

,,sALlcYt*rE-'

Salicylale

Nitroprussiate

f \tr"tn I
viat

Sodium

R3 |

31
L67

mmol/L

t15

KUUL

mmoul

at 600 nm > 0.100.

uREAsE

Urease

Don't use worldng reagent or diluted contents of vial R3 after expiry


date stated on the label ofthe kit.

BASE
SPECIMEN COLLECTION AilD HAilDLING (2)
llnhemolvsed serum or heoarinised olasma. Avoid fluoride or

7 mmoul
62 mmol/L

hypochlo.ite
hydroxide

Sodium
AfoE dildon

r Xi, R36/S, ldhtiry b yE and slln


! Awld .ontact rib skin and eves. Afrer .otrd rfr sBq tu
Imm.didly wth pbnry oI wei ft.r .oila.t with eFs, dnse immdadv ff
wat .and seek mdi.lr advi.e

S2&25.2G28

(- vi"l

Ra STaNDARD

Dbdv

urea 40 mg/dl (6.66 mmoul)

ammonium as antlcoagulant which interfere with the assay


Urea is stable in serum or plasma for :
. 24 h at room temperature.
. several days at 2.8t.
. at least 2-3 months freezed.
24!!!!g: diluted (1+19) with demineraiised water before assay
Urea is stable in urines for :

4 days at

2-8t.

SAFETY CAUTIONS

Add antibacterial agent as Thymol to improve the stability.

BIOLABO reagents are designated for professional, in vibo diagnostic


u5e,
. Vedty ihe integrity of the contents before use
Use adequate protections (overall, gloves, glasses)

F{TERFERENCES

.
.
.
.

Do not pipete by mouth.


ln case of contact with skin or eyes, thoroughly wash afiected areas
wirh plenty of water and seek medi(al advtce.
Reagents contain sodium azide (concentration < 0.Iolo) which may

.
.

when disposing.
lt4aterial Safety Data Sheet is available upon request.
Waste disposal I Respect legislation in force in the country'

ieiii

with copper and lead plumbing. Flush with plenty of water

13}

No interference of assayed substances (ascorbic acid,

bilirubin,

hemoglobin. triglycendes) with the test.


For a more comprehensive review of fact6E affecting this assay refer
to the publication of Young D.S.

MATERIAL REQUIRED BUT I{OT PROVIDED


l.Basic medical analysis laboratory equipment.
2.Nomal and patholoqical control sera

handled as potentially infectious, in


accoriance with good laboratory practices using appropriate

All

specimens should

be

precautions. Respect legislation in force in the country'

J8
Manulacturer Use by

Made in France

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diagnostc

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Temperature

lidtation

catalogue number

Latest revision : www.bioiabo:fr

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Revision : 26107/2011

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