Beruflich Dokumente
Kultur Dokumente
Plasmid DNA
Vaccine
For Staphylococcus aureus
Dr .M.Muruganandam
Dr.M.Muruganandam
Dedicated to My
Science Teacher Mr. Ramasamy
Preface
lab
Technician
for
kind
help
in
Arockiaraj,
chairman,
SMCET
for
M.Muruganandam
Contents
Biotherapy
Mutant Strain Vaccine
Mutant Strain plasmid
DNA Vaccine
Engineered plasmid
DNA Vaccine
Heat shock protein
Vaccine
Mixer Staph Vaccine
Recommendations
Bibliography
1. Biotherapy
In
2011,
Nobel
Prize
was
It
is
otherwise
called
etc
and
humoral
mediated
non-pathogenic
members
of
system
in
non-specific
way.
The
vaccines
are
biological
Live
attenuated
vaccine,
Peptide
methods
methods
are
are
employed,
injection,
the
topical
application,
oral
drops,
eye
drops,
bath
are
antigenic
part
of
pathogens,
compared
to
.Nowadays
cocktail
DNA
other
vaccines
vaccines
were
vitamin
reduce
stress.
The
DNA
vaccine
were
DNA.
Plasmids
are
used
as
vectors
in
for isolation
of
plasmid
DNA,
in
double
distilled
water
and
pathogens
such
as
Aeromonas
such
as
Pneumonia,
mastitis,
Methicillin
Resistant
has
become
more
antibiotics
especially
Methicillin,
worldwide
problem.
The
resistance
to
hydrophila,
Escherichia
coli,
vaccine
development.
These
works
second
Chapter
describes
After
that
mutant
strain
was
DNA
vaccine
preparation
of
Staphylococcus aureus.
Our studies showed that, plasmid
DNA vaccine is one of the best vaccines. So in
this attempt, try to develop a common plasmid
DNA
vaccine
for
staphylococcus
aureus,
typhi,
Escherichia
coli
and
mixed
well
and
delivers
through
stress
proteins
in
both
temperature
So
this
concentration
is
used
as
experimental
animal.
The
maximum immune
31
5
4
3
2
1
0
were
analyzed
on
the
basis
of
4000
3000
2000
1000
0
Lym
pol
maximum
lymphocyte
and
30
20
10
0
37
8
6
4
2
0
38
aureus
is
an
development
is
DNA
vaccines
Here
pathogen
was
was
cultured
in
our
laboratory.
For
was
injected
(200l)
through
blood
samples
were
collected
for
1-5
in
figures
shows
41
6
4
2
0
Sample-I
Sample-II
`
Fig-7: Mut. Stra. Plasmid DNA Vaccines
influence on RBC count (millions) of Albino
rats.
10000
5
5000
sample-I
sample-II
42
80
60
40
20
0
sample-I
sample-II
60
40
20
sample-I
sampe-II
43
30
20
10
sample-I
sample-II
vaccine
Staphylococcus
manufactured
for
the
aureus.
more
easily
infection
They
than
can
of
be
vaccines
the
storage,
transport
and
40
30
20
10
0
sampe-I
sampe-II
responses
15
10
5
sample-I
sample-II
8
6
4
2
0
47
DNA
vaccine
Staphylococcus aureus.
48
development
against
DNA was
digested
by different
were
double
digested
using
6000
5000
4000
3000
2000
1000
0
50
1
0
5
60
40
20
0
1
2
3
4
51
Source
Recognition
Sequence
1.
EcoR-1
E. coli RY 13
AATTC 3
2.
5GG CC 3
III)
3.
Pst-I
CTGCAG
5.
Bam H Bacillus
GATCC 3
Hind
III
anyloliquefacies H
Haemophilus
influence Rd
5....
AGCTT...3
3....TTGA
A....5
52
potential
of
virulence
was
80
60
40
20
0
5
4
3
2
1
0
56
1
2
5
0
3
4
30
20
10
0
1
2
3
4
57
10
8
6
4
2
0
vaccines
by
various
Restriction
58
10
6000
1
2
4000
2000
0
8
4
7
5
6
1
2
3
4
5
6
59
60
10
40
9
20
0
8
1
2
3
4
5
6
10
9
5
4
3
2
1
0
1
2
3
4
7
5
6
60
4
10 3
2
9
1
0
8
1
2
3
4
5
6
10
10
0
8
4
7
5
6
61
10
30
1
2
20
10
0
8
4
7
5
6
10
9
10
8
6
4
2
0
1
2
3
4
7
5
6
One
intramuscular
injections.
After
8000
6000
4000
2000
0
65
2
poly
lym
4
2
66
10
5
The
So it is highly
68
temperatures
with
various
time
physiological
saline
and
injected
as
8000
6000
4000
2000
Temp-50
Temp-55
Temp-50
Temp-55
Compared
to
both
temperature
55
50
45
Temp-50
Temp-55
73
Generally,
microorganisms,
release
If
rising
the
environmental
Temp-50
Temp-55
74
2
1.5
1
0.5
0
Temp-50
Temp-55
10
8
6
4
2
0
Temp-50
Temp-55
30
20
10
Temp-50
Temp-55
Temp-50
Temp-55
76
at
55 0 C
temperature
treatment
are
highly
suitable
for
vaccine
preparations and also useful to new bioadjuvant preparation for staph DNA vaccines.
77
Optimization work-I
Acetone
79
various quantities to
LYM
POLY
EOIS
15
10
5
81
29
28
27
26
The immune
2.5
2
1.5
1
0.5
0
recommended
to
further
protein
vaccine
Optimization-II
In this study graded level of staph Hsp
vaccine was provided to albino rats for
optimization study.
6000
4000
2000
1
2
POLY
LYM
EOIS
86
15
10
5
2.5
2
1.5
1
0.5
0
88
food.
Many
vaccines
were
were
isolated
and
Heat
stress
protein
vaccine
vaccine
preparation,
all
these
vaccines
4
3
2
1
0
1
2
3
4
8000
6000
4000
2000
0
1
2
40
20
LYM
POLY
3
4
92
15
10
5
0
1
2
3
4
40
30
20
10
0
2
1.5
1
0.5
0
this
study,
various
staph
94
staph
killed
vaccines
stimulate
98
Bibliography
Moran.G.J.
,Krishanadasan.A,
Gorwitz.R.J.,
Fosteim.G.E., Mc. Dougal L.K., Carey.R.B. (2006)
Methicillin- resistant S. aureus infections among
patients in the emergency department.J of medicine.
val. 355 pp. 666-74.
Kerttuls.A, Lyytikainen .O , S.Vgopio varkilla.J.
(2004) changing epidemiology of Methicillin
resistant Staphylococcus aureus in Finland.J . horp.
Inf. 58: 109 114.
Lawrence.C. , Cosseron.M., Durand.P, Costa.Y.,
Leclerig.R. (1996), consecutive isolation of
homologous strains of Methicillin-resistant and
Methicillin-susceptible Staphylococcus aureus form a
hospitalized child. J. Horp. Inf. 33 :49-53.
Liu.Y, Wang.H, Dlu.N, Sten.E, Chen.H, Niu.J (2009).
Molecular evidence for spread of two major
Methicillin-resistant staphylococcus aureus clores
with a unique geographic distribution in chinese
hospitals, Antimicrob. Agents. Chemother. 53: 512518.
Michael.S., Truchiya.H, Miyazaki.J, fujiwara.S,
Yamaguchi. R, Kureshiro.. (1996) Antibacterial
activity of hydroxy chaicore against Methicillinresistant Staphylococcus aureus. Int. J.Antimicro.b.
Agents. 6: 227-231.
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Scribel.L.V.,
Silva-carvalho.M.C.,
Souza.R.R.,
Superti.S.V., Kvitko.C.H.C., Figuerieredo.A.M.S.
(2009). clinical and molecular epidemiology of
Methicillin-resistant staphylococcus aureus carrying
sec mec IV in a university hospital in portoalegre.
brazil. Diag. Microbiol. Inf. Dis. 65:457-461.
Razieh Amini, Abdulamir.A.S., Fatemeh Jahanshiri,
Lee Chye Shan, Alihematian, Yasaman Amini,
Zamberi Sekawi and Farid Azizi Jalilian. (2012).
Isolation and Identification of Methicillin-resistant
Staphylococcus aureus from students coins
Aftrican.J. Biotech. Vol.II (50). pp. 11143-11149.
Climo.M.E., Patron.R.L. and Archer.G.L. (1999)
combinations of encomycin and beta- lactams are
synergistic against staphylococci with reduced
susceptibilities to vanomy(in. J. Che mot. vol. 43 pp.
Ligi, Liu Y.K., Mak.J.Y. Chen.L.L. and Lee.W.M.
(2005) Thermal responses of rat fibre sets stably
transfected with the human 70 K De heat shock
protein encoding gene. vol. 88. pp. 1681 1685.
Yues Le Loir, Florence Aron and Michal Gautier
(2003). Staphylococcus aureus and food poisoning
Geret mole Res. Vol.2 No.1 .pp. 63-76.
Cunta.B.A.(2006), New uses for older antibiotics
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doxyiyilire and mino cycline 90: 1089 1097.
100
101
102
103
Dr.M.Muruganandam is
working in Einsteein Bio-Engineering
Research Foundation. He is an Editor
of African journal of Biotechnology and
International journal of Medicine and
Biomedical
Research.
He
is
also
104