Beruflich Dokumente
Kultur Dokumente
5.1.
OBJECTIVE
5.2.
INTRODUCTION
purpose
in
small
percentage.
Many
of
the
5.3.
5.4.
5.5.
Methanol
5.6.
5.7.
7.5% NaHCO3
5.8.
Gallic acid
5.9.
DPPH solution
5.10.
Ascorbic acid
5.11.
Vortex
5.12.
Water bath
5.13.
Test tube
5.14.
Centrifuge
5.15.
Spectrophotometer
5.16.
5.17.
PROCEDURE
5.18.
5.18.1.
Sample extraction
One gram of each sample transferred into a test tube, added 3 mL
of methanol and vortex the mixture for 30 sec.
The test tube was capped and incubated in water bath (60C) for 15
min and during incubation, the test tube was vortex twice.
The test tube was centrifuge for 5 min and transferred the
supernatant to a 10 mL volumetric flask.
The above step was repeated and combined with previous
supernatant and adjusted to 10 mL .
5.19.
5.19.1.
extracts.
0.5 mL of methanolic solution of extract was added with 2.5 mL of
10% Folin Ciocaltues reagent dissolved in water and 2.5 mL 7.5%
NaHCO3.
Blank containing 0.5 mL methanol was prepared, with 2.5 mL of
10% Folin Ciocaltues reagent dissolved in water and 2.5 mL 7.5%
NaHCO3.
The sample was incubate at 45C for 30 min and the absorbance
was measured using spectrophotometer at 765 nm.
The procedure for standard solution of gallic acid and construct
calibration curve was repeated.
The concentration of total phenolics contents in extract from
calibration curve was calculated.
5.20.
5.20.1.
Antioxidant activity
0.1 ml of methanolic extract with 3.9 mL of 6 10 -5 mol/L methanol
DPPH solution.
Blank containing 0.1 ml of methanol and 3.9 ml of .9 mL of 6 10 -5
mol/L methanol DPPH solution was prepared.
The mixture was mixed thoroughly and incubated for 30 min in the
dark.
The absorbance was measured at 515 nm.
5.21.
RESULTS
5.22.
5.22.1.
GALLIC ACID STANDARD CURVE
5.23.
5.24. Concentration (mg/L)
5.26. 0
5.28. 20
5.30. 40
5.32. 60
5.34. 80
5.36. 100
5.25.
5.38.
f(x) = 0.01x
R = 0.99
0.7
0.6
Absorbance at A765nm
Absorbance at 765nm
0.5
0.4
0.3
0.2
0.1
0
0 20 40 60 80 100120
Concentration (mg/mL)
5.39.
y
5.42. concentration of t otal phenolic content , x= 0.0094
5.43. For group B5:
0.031
0.0094
5.44. x=3.298
5.45.
5.46.
5.47. G
roup
5.50.
5.53.
5.56.
5.59.
5.62.
5.65.
1
2
3
4
5
6
5.70.
inhibition of DPPH =
5.71.
5.72.
Grou
p
5.76.
1
5.80.
2
5.84.
3
5.88.
4
5.73. Absorbance
control
5.77. 4.00
5.81. 4.00
5.85. 4.00
5.89. 4.00
5.74. Absorbance at
515nm, sample
5.75. % inhibition of
DPPH
5.78. 2.352
5.79. 41.2
5.82. -
5.83. -
5.86. -
5.87. -
5.90. -
5.91. -
5.92.
5
5.96.
6
5.93. 4.00
5.97. 4.00
5.100.
5.94. 2.898
5.95. 27.55
5.98. -
5.99. -
5.101.
DISCUSSION
5.102.
total
phenolic
content
using
Folin-Ciocalteaus
method,
scavenging activity on DPPH radicals, reducing power assay and carotene method) to evaluate the antioxidant activity of plant
material. In this study, green tea was extracted with methanol and
water extracts. In this experiment, only the total phenolic content
using Folin-Ciocalteaus method and scavenging activity on DPPH
radicals.
5.103.
5.104.
Mo(VI)+eMo(V)
5.106.
5.107.
y
0.0094
5.108.
5.109.
are shown in Table 2. The highest amount was found in the Group 1
with 126.702 mg of GAEs/g of extract, followed by the Group 3
(119.680 mg of GAEs/g of extract), Group 5 (61.277 mg of GAEs/g of
extract) and Group 2 and 6 (52.34 mg of GAEs/g of extract, 3.298 mg
of GAEs/g of extract respectively). The high phenolic content might
contribute toward its antioxidant activities.
5.110.
5.111.
5.115.
5.116. CONCLUSION
5.117.
5.118.
from the present study indicated that the extract of green tea has the
highest total phenolic content is 126.702 mg of GA / g of extract. This
indicated that the antioxidant activity of the green tea extract was well
correlated with the content of its phenolic compounds. In conclusion,
this study suggested that green tea is a potential source of natural
antioxidants. However, further investigations on in vivo antioxidant
activities are highly recommended.
5.119. REFERENCES
5.120.
5.121.
Rice Evans, C., Millerm N., and Paganga, g. (1997). Antioxidant properties
of phenolic compounds. Trends in Plant Science 2(4) : 152 - 159.
5.122.
5.123.
Brand Williams, W., Cuvelier, M. E. and Berset, C. (1995). Use of a free
radical method to evaluate antioxidant activity. LWT Food Science and Tecnology 28(1)
: 25 30.
5.124.
5.125.
Stankovic M. S. Total phenolic content, flavonoid concentration and
antioxidant activity of Marrubium peregrinum L. extracts. Kragujevac J Sci, 33(2011), 63
72.
5.126.
5.127.