SANTA CRUZ BIOTECHNOLOGY, INC.

Integrin V/3 (23C6): sc-7312

BACKGROUND

STORAGE

Integrins are heterodimers composed of noncovalently associated transmembrane and subunits. The 16 and 8 subunits heterodimerize to produce
more than 20 different receptors. Most integrin receptors bind ligands that
are components of the extracellular matrix, including Fibronectin, collagen
and Vitronectin. Certain integrins can also bind to soluble ligands such as
Fibrinogen, or to counterreceptors on adjacent cells such as the intracellular
adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve
to cross-link or cluster integrins by binding to adjacent integrin receptors;
both receptor clustering and ligand occupancy are necessary for the activation
of integrin-mediated responses. In addition to mediating cell adhesion and
cytoskeletal organization, integrins function as signaling receptors. Signals
transduced by integrins play a role in many biological processes, including
cell growth, differentiation, migration and apoptosis.

Store at 4 C, **DO NOT FREEZE**. Stable for one year from the date of
shipment. Non-hazardous. No MSDS required.

REFERENCES
1. Hynes, R.O. 1992. Integrins: versatility, modulation, and signaling in cell
adhesion. Cell 69: 11-25.
2. Clark, E.A., et al. 1995. Integrins and signal transduction pathways: the
road taken. Science 268: 233-239.

CHROMOSOMAL LOCATION
Genetic locus: ITGB3 (human) mapping to 17q21.32, ITGAV (human) mapping
to 2q32.1.

DATA

Integrin V/3 (23C6): sc-7312. Immunoperoxidase

staining of formalin fixed, paraffin-embedded human
urinary bladder tissue showing membrane staining of
urothelial cells.

Integrin V/3 (23C6) PE: sc-7312 PE. FCM analysis

of human peripheral blood leukocytes. Black line
histogram represents the isotype control, normal
mouse IgG1: sc-2866.

SELECT PRODUCT CITATIONS

1. Bildirici, I., et al. 2001. A prospective evaluation of the effect of salpingectomy on endometrial receptivity in cases of women with communicating hydrosalpinges. Hum. Reprod. 16: 2422-2426.
2. Pathak, A.K., et al. 2007. Ursolic acid inhibits STAT3 activation pathway
leading to suppression of proliferation and chemosensitization of human
multiple myeloma cells. Mol. Cancer Res. 5: 943-955.

SOURCE

3. Wang, W.J. 2008. Agglucetin, a tetrameric C-type lectin-like venom protein, regulates endothelial cell survival and promotes angiogenesis by
activating integrin v3 signaling. Biochem. Biophys. Res. Commun. 369:
753-760.

PRODUCT

4. Thews, O., et al. 2009. Impact of reactive oxygen species on the expression
of adhesion molecules in vivo. Adv. Exp. Med. Biol. 645: 95-100.

Integrin V/3 (23C6) is a mouse monoclonal antibody raised from osteoclasts

from osteoclastomas of human origin.

Each vial contains 200 g IgG1 in 1.0 ml of PBS with < 0.1% sodium azide
and 0.1% gelatin.
Available as phycoerythrin (sc-7312 PE) or fluorescein (sc-7312 FITC)
con-jugates for flow cytometry, 100 tests.
Available as Alexa Fluor 405 (sc-7312 AF405), Alexa Fluor 488 (sc-7312
AF488) or Alexa Fluor 647 (sc-7312 AF647) conjugates for flow cytometry
or immunofluorescence; 100 g/2 ml.
Alexa Fluor is a trademark of Molecular Probes, Inc., Oregon, USA

APPLICATIONS

Integrin V/3 (23C6) is recommended for detection of Integrin V/3 of

human origin by immunoprecipitation [1-2 g per 100-500 g of total protein
(1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution
range 1:50-1:500), immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500) and flow cytometry
(1 g per 1 x 106 cells).

Molecular Weight of Integrin V: 125-135 kDa.

1.800.457.3801

6. Chang, C.W., et al. 2009. The IL-8 production by streptococcal pyrogenic

exotoxin B. Exp. Biol. Med. 234: 1316-1326.
7. Blair, H.C., et al. 2009. Osteopetrosis with micro-lacunar resorption
because of defective integrin organization. Lab. Invest. 89: 1007-1017.

8. Wang, W.J. 2010. Acurhagin-C, an ECD disintegrin, inhibits integrin v3mediated human endothelial cell functions by inducing apoptosis via caspase-3 activation. Br. J. Pharmacol. 160: 1338-1351.
9. Seoane, A.I., et al. 2010. The mojastin mutant Moj-DM induces apoptosis
of the human melanoma SK-Mel-28, but not the mutant Moj-NN nor the
non-mutated recombinant Moj-WN. Toxicon 56: 391-401.

RESEARCH USE
For research use only, not for use in diagnostic procedures.

Molecular Weight of Integrin 3: 125 kDa.

Santa Cruz Biotechnology, Inc.

5. Wang, M.Y., et al. 2009. Connective tissue growth factor confers drug
resistance in breast cancer through concomitant up-regulation of Bcl-xL
and cIAP1. Cancer Res. 69: 3482-3491.

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