RollNo.......................

TotalNo.ofQuestions:09

TotalNo.ofPages:03

B.Sc.IBTEXAMINATION
MICROBIOLOGY
SUBJECTCODE:102
Time:02Hours

MaximumMarks:60

InstructiontoCandidates:
1)SectionAiscompulsory.
2)AttemptanythreequestionsfromSectionB.
3)AttemptanytwoquestionsfromSectionC.
SectionA

Q1)
I. Which of the following is/are not a gram-positive bacteria?
(a) Streptococci

(b) Pseudomonas

(c) Mycobacteria

(d) None of these

II.

(25x1=25)

Lag phase is also known as

(a) Period of initial adjustment

(b) Transitional period

(c) Generation time

(d) None of these

III.

The solidifying agent commonly used in preparation of media is/are

(a) Agar

(b) Silica Gel

(c) Both A & B

(d) None of these

IV.

All of the following are features of prokaryotes except?

(a) Nitrogen fixation

(b) Chemosynthesis

(c) Sexual reproduction

(d) Locomotion

V.

Which of the following is/are included in Kingdom Prokaryotae?

(a) Bacteria

(b) Protozoa

(c) Fungi

(d) All of these

VI.

The Archaea include all of the following except

(a) Methanogens

(b) Halophiles

(c) Thermoacidophiles

(d) Cyanobacteria
Contd

VII.

When a virus enters a cell but does not replicate immediately, the situation is
called
(a) Lysogeny

(b) Fermentation

(c) Symbiosis

(d) Synergism

VIII.

Cell size and cell number can be measured using

(a) Hemocytometer

(b) Coulter counter

(c) Chemostat

(d) None of these

IX.

Ethylene oxide is used to destroy or kill which of the following microbes?

(a) Bacteria

(b) Spore

(c) Fungi

(d) All of these

X.

Microbes can be removed from a liquid solution by the process of

(a) Filteration

(b) Freeze-drying

(c) Osmosis

(d) Desiccation

Fill in the blanks

XI. Mycology is the study of fungi
XII.

Blue-Green bacteria called cyanobacteria.

XIII.

The first step in infection of a host bacterial cells by a phage is adsorption

XIV.

The primary stain of Gram's method is Crystal voilet

XV.
XVI.
XVII.

The fruiting body of a mushroom is called basidiocarps

The five-kingdom system of classification was set up by Robert Whittaker
Organisms, using organic compounds as electron donors are called
organotrophs

XVIII.
XIX.

The equation used to obtain bacterial population by binary fission is

The process of making an object free from living organisms including
bacterial and fungal spores and viruses is known as sterilization

XX.

Gram staining was introduced by Christian gram

True and false

XXI. The fruiting body of a mushroom is called ascocarps
Contd.
.
XXII.

The thallus of a mold consists of mycelium and spores

XXIII.

Gram-negative bacteria may contain fimbriae

XXIV.

The reactions of the cell that are carried out for capturing energy are called
catabolism

XXV.

The period between inoculation of bacteria in a culture medium and

beginning of multiplication is known as
Section - B

(5x 3 = 15)
Q 2) Describe briefly the factors affecting microbial growth.
Q 3) What is the principle of optical density measurement of bacterial cell?
Q 4) What is the difference between a simple and differential stain?
Q 5) What is the economic importance of cyanobacteria?
Q 6) Describe briefly different phases of bacterial growth curve of bacteria.
Section - C
(2 x 10 = 20)
Q 7) What is generation time? Describe briefly the methods of measurement of
growth in bacteria.
Q 8) Explain the methods of sterilization
Q 9) Classify different kinds of culture media and give one example