Beruflich Dokumente
Kultur Dokumente
Heavy chain
Heavy chain
Light chain
Chapter 4
See Fig. 4-9
Page 88
Light chain
See p97
One gene
One protein
Non-B cell
Southern Blot
Today:
1. Gene (DNA) rearrangements
2. RNA splicing
p. 111
2.
1.
DNA
pre-mRNA
mRNA
protein
Ig Locus Rearrangement
Sequence-specific: recombination signal is a
conserved heptamer (CACAGTG), a spacer (12 or
23 non-conserved bases), and a conserved
nonamer (ACAAAAACC)
12/23 Rule: Recombination machinery always
joins a gene segment with a 12-bp spacer to
another with a 23-bp spacer
This ensures that the correct gene segments get
joined (no V-V, H chain always has D between V
and J, etc.)
RAG = recombinase
(Disregard Inv Join)
Junctional Diversity
Junctional Flexibility
(many)
IgD
Surface Ig expression
Encodes
transmembrane
domain
Somatic Hypermutation
After rearrangement !!! - [ in response to antigen ]
Increased mutations
as response continues
Affinity maturation !
See Chapter 4
Cell number
Fluorescence intensity