Chere Petty M.S.

Bethesda, Maryland 20816

Phone: 301-367-8408
E-mail: cherepetty@gmail.com

Research Experience:
2004-2016
Manager of Keith R. Porter Core Imaging Facility
University of Maryland Baltimore County

1998-2004
Laboratory Manager, Principal Investigator: Dr. D. Blumberg
University of Maryland Baltimore County

Summer of 1997
Laboratory Volunteer, Principal Investigator: Dr. D. Blumberg
University of Maryland Baltimore County

Education:
M.S.

2004

University of Maryland Baltimore County

Biological Sciences

B.S.

1997

University of Maryland Baltimore County

Biological Sciences

Interests:
Interacting and advising on image acquisition and analysis with different labs on a wide variety
of projects.
Helping develop protocols for a variety of different microscopes.
Supporting research through microscopy.
Keeping current on new technology and techniques.

Equipment:
Leica SP5 TCS confocal.
Leica M205 FA Stereoscope.
Polarized Light Microscopy
Wide-Field Microscopy
Scanning Electron Microscopy
Zeiss 10CA Transmission Electron Microscope.
MetaMorph software.
Volocity software.
iTEM software.
Microsoft Office
Excel spreadsheets
Powerpoint presentation

Teaching Experience:
Developmental Cell Lab to use fluorescent compound microscopes and fluorescent probes/stains.
2009 to 2016

Phage Hunter Lab negative staining and Transmission Electron Microscopy.

2010 to 2016

BioEngineering Lab to use Leica SP5 TCS confocal to image cells in a matrix.
2011 to 2012

Howard Hughes Medical Institute SEA-PHAGE faculty workshop negative staining and
Transmission Electron Microscopy.
Cell Lab to use Leica SP5 TCS confocal and fluorescent probes/stains.
2012 to 2016

Professional Development:
Attend 3D Microscopy of living Cells at University of British Columbia, Vancouver, Canada.
June 2000

Attend 3D Image- Processing course at University of British Columbia, Vancouver, Canada.

W. M. Keck Center for Cellular Imaging. FRET workshop.
July 2000

Affiliations/Memberships:
American Association for the Advancement of Science
1998 to present

The American Society For Cell Biology

1998 to 2009

Microscopy Society of America

2000 to present

Publications:
Noratel, E.F., Petty, C.L., Kelsey, J.S., Cost, H. N., Basappa, N. and D.D. Blumberg. 2012 The
adhesion modulation protein, AmpA localizes to an endocytic compartment and influences cell
migration, substrate adhesion, actin polymerization and endocytosis. BMC-Cell Biology. 13:29
doi:10.1186/1471-2121-13-29

Blumberg, D. D., Ho, H. N., Petty,C. L., Varney, T. R., Gandham, S. 2002 AmpA, modular
protein containing disintegrin and ornatin domains, has multiple effects on cell adhesion and cell
fate specification. J. Muscle Research and Cell Motility 23:817-828

Casademunt, E., Varney, T. R., Dolman, J., Petty, C., Blumberg, D. D., 2002 A gene encoding a
novel anti-adhesive protein is expressed in growing cells and restricted to anterior-like cells
during development in Dictyostelium. Differentiation 70:23-35

Varney, T. R., Ho, H., Petty, C., Blumberg, D. D., 2002 A novel disintegrin domain protein
affects cell fate specification and pattern formation in Dictyostelium Development 129:23812389

Varney, T. R., Casademunt, E., Ho, H., Petty, C., Dolman J., and Blumberg, D. D., 2002 A
novel Dictyostelium gene encoding multiple repeats of adhesion inhibitor-like domains has
effects on cell-cell and cell-substratum adhesion. Development Biology 243: 226-248

Publications that include data obtained in Keith R. Porter Imaging Facility:

Dolan, B., K. Gibbs, Jr., Ostrand-Rosenberg, 2005 Dendritic cells activate tumor-specific T
lymphocytes by presenting tumor-generated peptide-MHC complexes. J. Immunology

Cheng, Q., Pappas, V., Hallmann, A., Miller, S. M., 2005 Hsp70A and GIsA interact as partner
chaperones to regulate asymmetric division in Volvox. Dev. Biol. Oct 15;286(2):537-48

Mascari, L. M., Ross, J. M., 2003 Quantification of Staphylococcal-Collagen binding

interactions in whole blood by use of a confocal microscopy shear-adhesion assay. J Infect Dis
188(1):98-107

Harrington, M., Hong, E., Fasanmi, O., Brewster, R., 2007 Cadherin-mediated adhesion
regulates posterior body formation. BMC Dev Biol., 7:130

Lin, W., Ezekwe JR., E, A. D., Zhao, Z., Liman, E, Restrepo, D., 2008 TRPM-5expressing
microvillous cells in main olfactory epithelium. BMC Neurosci Nov24;9:114

Acosta, M. A., Ymele-Leki, P., Kostov, Y. V., Leach, J. B., 2009 Fluorescent microparticles for
sensing cell microenvironment oxygen levels within 3D scaffolds. Biomaterials 30;3068-3074

Bosch, J., Iheagwara, U., Reid, S., Srivastava, M., Wolf, J., Lotem, B., Ksander, B., OstrandRosenberg, S., 2010 Uveal melanoma cell-based vaccines express MHC II molecules that traffic
via the endocytic and secretory pathways and activates CD8(+) cytotoxic, tumor-specific T cells.
Cancer Immunol & Immunother 59:103-112

Dolan, B., Gibbs Jr, K., Ostrand-Rosenberg, S., 2006 Tumor-specific CD4+T cells are activated
by cross-dressed dendritic cells presenting peptide-MHC class II complexes acquired from
cell-based cancer vaccines. J. Immunol 176:1447-1455 (Highlighted In this issue)

Dolan, B., Gibbs Jr., K., Ostrand-Rosenberg, S., 2006 Dendritic cell cross-dressed with peptideMHC class I complexes prime CD8+T lymphocytecs. J. Immunol. 177:6018-6024

Jayachandran, P., Hong, E., Brewster, R., 2010 Labeling and imaging cells in the zebrafish
hindbrain. J. Vis Exp. (41) pii 1976. doi:10.3791/1976

Hong, E., Jayachandran, P., Brewster, R., 2010 The polarity protein Pard3 is required fro
centrosome positioning during neurulation. Dev Biol., 341(2):335-45 Epud 2010 Feb 6

Harrington, M. J., Chalasani, K., Brewster, R., 2009 Cellular mechanisms of posterior neural
tube morphogenesis in zebrafish. Dev. Dyn., 239(3):747-62

Harrington, M. J., Hong, E., Brewster, R., 2009 Comparative analysis of neurulation: first
impressions do not count. Mol Reprod Dev., 76(100:954-65

Chalasani, K., Brewster, R., 2010 Mol Reprod Dev. 77(1):1

Chalasani, K., Brewster, R., 2011 N-cadherin-mediated adhesion restricts cell proliferation in
the dorsal neural tube. Molecular Biology of the Cell, 22(9):1505-15. PMC3084673

Ng, G., Seabolt, S., Zhang, C., Salimian, S., Watkins, T. A., Lu, Hua 2011 Genetic dissection of
salicylic acid-mediated signaling networks in Arabidopsis. Genetics

Cronin, T. W., Feller, K., Sensory ecology of vision. Biology of Crustacea vol. 3 Academic
Press, New York

Cronin, T. W., 2010 Polarized-light vision in land and aquatic animals. Encyclopedia of the Eye,
Vol. 3( Darelne A. Dartt, editor) Oxford: Academic Press, pp. 461-468

Cronin, T. W., Chiou, T. H., Caldwell, R. L., Roberts, N., Marshall, J., 2009 Polarization signals
in mantis shrimps. Proceedings of SPIE 7461. Polarization Science and Remote Sensing IV. (
J.A. Shaw and J.S. Tyo, eds.)Press,

Bellingham, WA. pp. 74610C-I 74610C-10

Chiou, T. H., Calwell, W. L., Hanlon, R., Cronin, T. W., 2008 Fine structure and optical
properties of biological polarizers in crustaceans and cephalopods. Proceedings of SPIE 6972.
Polarization: Measurement, Analysis, and Remote

Sensing VIII (D.B. Chenault and D.H. Goldstein, eds.) SPIE Press, Bellingham, WA. pp.
697203-1 697203-10

Chiou, T. H., Klenlogel, S., Cronin, T. W., Callwell, R. L., Lofle, B., Siddiqi, A., Goldizen, A.,
Marshall, J., 2008 Circular polarization vision in a stomatopod crustacean. Current Biology
18:429-434

Chiou, T. H., Mathger, L. m., Hanlon, R. T., Cronin, T. W., 2007 Spectral and spatial properties
of polarized light reflections from the arms of squid and cuttlefish. J. Exp. Bio 210:3624-3635

Chiou, T. H., Cronin, T. W., Caldwell, R. L., Marshall, J., 2005 Biological polarized-light
reflectors in stomatopods crustaceans. Proceedings of SPIE 5888. Polarization Science and
Remote Snsing II (J. A. Shaw and J. S. Tyo, eds.) SPIE Press, Bellingham, WA, pp. 58881B1-9

Song, H., Cai, Z., Hongseok, N., Bennett, D., 2010 Chaotic mixing in microchannels via low
frequency switching transverse electroosmotic flow generated on integrated microelectrodes.
Labs-on-a-Chip, vol.10, 74-740

Chornoguz, O., Grmai, L., Sinha, P., Artemenko, K. A., Zubarev, R. A., Ostrand-Rosenberg, S.,
2011 Proteomic pathway analysis reveals inflammation increases myeloid-derived suppressor
cell resistance to apoptosis. Mol. Cell Protemics 10: epud ahead of print.

Chen, H., Nandi, A. K., Li, X., Bieberich, C. J., 2002 NKX-3.1 interacts with prostste derived
ETS factor and regulates the activity of the PSA promoter. Cancer Research 62338-340

Riberio, A., Vargo, E., Powell, E., Leach, J. B., 2011 Substrate three-dimensionality induces
elemental morphological transformation of sensory neurons on a physiologic timescale. Tissue
Engineering.

Acosta, M., Ymele-Leki, P., Kostov, Y., Leach, J. B., 2009 Fluorescent microparticles for
sensing microenviroment oxygen levels within 3D scaffolds. Biomaterials 30:3069-74 [PMID
19285719]

Keshet, B., Gray, J. J., Good, T. A., 2010 Structurally distinct toxicity inhibitors bind at common
loci on a-amyloid fibril. Protein Sci. 2010 Dec; 19(12):2291-304.

Keshet, B., Yang, I. H., Good, T. A., 2010 Can size alone explain some of the differences in
toxicity between beta-amyloid oligomers and fibrils? Biotechnology and Bioeng. 106(2):333-7

Ramos, I., Fabris, D., Qi, W., Fernandez, E. J., Good, T. A., 2009 Kinetic study of beta-amyloid
residue accessibility using reductive alkylation and mas spectrometry. Biotechnol. Bioeng.
104(1)181-92.

Zhang, A., Qi, W., Good, T. A., Fernandez, E. J., 2009 Structural differences between Abeta(1-40) intermediate oligomers and fibrils elucidated by proteolytic fragmentation and
hydrogen/deuterium exchange. Biophys. J. 96(3):1091-104

Wei, Q., Zhang, D., Patel, D., Harrington, J., Zhao, L., Schaefer, D., Good, T. A., Fernandez, E.,
2008 Simultaneous monitoring of peptide aggregate distributions, structure, and kinetics using
amide hydrogen exchange: Application to A-Beta(1-40) fibrillogenesis. Biotechnol Bioeng.
100(6): 1214-27.

Beier, H., Cowan, C., Chow, I. H., Pallikal, J., Henry, J., Benford, M., Jackson, J., Godd, T.A.,
Cote, G., 2007 Application of Surface Enhanced Raman Spectroscopy for Detection of Beta
Amyloid using nanoshells. Plasmonics 2:55-64,

Karr, J. W., Szalai, V. A., 2008 The Copper(II) Binding Site in Monomeric and Fibrillar Forms
of the Amyloid-b Peptide of Alzheimers disease. Biochemistry 47, 5006-5016.

Shearer, J., Callan, P. E., Tran, T., Szalai, V. A., 2010 Cu K-edge X-ray absorption
spectroscopy reveals differential copper coordination within amyloid-B oligomers compared to
amyloid-B monomers. Chem Commun (Camb) 46:9137-9

Johnson, M. A., Ross, J. M., 2008 Staphylococcal shear conditions in whole blood studies. Ann.
Biomed. Eng., 36:349-55.

Ymele-Leki, P., Ross, J. M., 2007 Erosion from Staphylococcus aureus biofilms grown under
physiologically relevant fluid shear yields bacterial cells with reduced avidity to collagen. Appl.
Env. Microbiol., 73:1834-41.

VanDamme, N.S., Topoleski, LDT., 2005 Control of surface morphologyof carbide coating CoCr-Mo implant alloy. Journal of Materials Science, Materials in Medicine, 16: 647-654

Kelsey, J. S., Fastman, N. M., Blumberg, D. D., 2012 Evidence of an evolutionarily conserved
LMBR1 domain-containing protein that associates with endocytic cups and plays a role in cell
migration in Dictyostelium discoideum . Eukaryote Cell 11(4) 401-416 doi: 1128/EC.0518611(Journal cover photo is from this paper)

Kelsey, J. S., Fastman, N. M., Noratel, E. F., Blumberg, D. D., 2012 Ndm, a coiled-coil domain
protein that suppresses macropinocytosis and has effects on cell migration. Mol. Biol. Cell
23:3407-3419 doi 10.1091/mbc.E12-05-0392.

Kelsey, J. S., Blumberg, D. D., 2013 A SAP-domain containing proteinshuttles between thte
nucleus and cell membranes and regulates adhesion and migration in D. discoidium .
BiologyOpen 0:1-11 doi:10.1242/bio.20133889

Ribeiro, A., Vargo, S., Powell, E., Leach, J. B., 2012 Substrate three-dimensionality induces
elemental morphological transformation of sensory neurons on a physiologic timescale. Tissue
Engineering 18:93-102

Chornoguz, O., Grmai, L., Sinha, P., Artemenko, K., Zubarev, R., Ostrand-Rosenberg, S., 2011
Proteomic pathway analysis reveals inflammation-induced myeloid-derived suppressor cells
have enhanced resistance to apoptosis. Mol. Cell Proeomics 10:M110002980 PMCID:
PMC3047151

Ostrand-Rosenberg, S., Sinha, P.,Chornoguz, O., Ecker, C., 2012 Regulating the suppressors:
Apoptosis and inflammation govern the survival of tumor-induced myeloid-derived suppressor
cells)MDSC). Cancer Immunol Immunother 61:1319-1325

Haile, S., Bosch, J., Agu, N., Zeender, A., Somasundaram, P., Srivastava, M., Rodel, S., Wolf, J.,
Ksander, B., Ostrand-Rosenberg, S., 2011 Tumor cell programmed death ligand-1-mediated T
cell suppression is overcome by co-expression of CD80. J. Immunol. 186:6822-6829. PMCID
3377446

Ostrand-Rosenberg, S., Sinha, P., Beury, D., Clements, V., 2012 Cross-talk between myeloidderived immune suppression. Sem. Cancer Biol. 22:275-281

Cronin, T.W., Feller, K., 2013 Sensory ecology of vision. In: Biology of Crustacca, vol. 3 (C.
Derby and M. Thiel, eds.) Academic Press, New York

Porter, M. L., Speiser, D. L., Zaharoff, S., Caldwell, R. L., Cronin, T. W., 2013 The evolution of
complexity of visual systems of stomatopods: insights from transcriptomics. Integrative and
Comparative Biology

Roberts, N. W., Marshall, N. J., Cronin, T. W., 2012 High levels of reflectivity and pointillist
structural color in fish, cephalopods, and beetles (letter). Proceedings of the National Academy
of the USA 109:E3387

Cronin, T. W., 2012 Visual optics: Accommodatioons in a splash. Curr. Biol. 22:R871-R873

Frank, T.M., Johnsen, S., Cronin, T.W., 2012 Light and vision in the deep-sea benthos: II.
Vision in deep-sea crustaceans. Journal of Experimental Biology 215:3344-3353

Bishoff, B., Nickle, B., Cronin, T.W., Vaelasquez, S., Fasick, J. L., 2012 Deep-sea and pelagic
rod visual pigments identified in mysticete whales. Visual Neuroscience 29:95-103

Chiou, T. H., Place, A. R., Caldwell, R. L., Marshall, N. J., Cronin, T. W., 2012 A novel
function for a carotenoid: astaxanthin used as a polarizer for visual signaling in a mantis shrimp.
Jouranl of Experimental Biology 215:584-589

Porter, M. L., Blasic, J. R., Bok, E. G., Cameron, E. G., Pringle, T., Cronin, T. W., Robinson,
P.R., 2012 Shedding new light on opsin evolution. Proceedings of the Royal Society of London
B 279:3-14

Marshall, J., Cronin, T. W., 2011 Polarization vision. Current Biology 21:R101-R105

Marshall, J., Cronin, T.W., Wehling, M. F., 2011 Introduction: New directions in the detection
of polarized light. Philosophical Transactions of the Royal Society of London B 366:615-616

Cronin, T. W., Marshall, J., 2011 Patterns and properties of polarized light in air and water.
Philosophical Transactions of the Royal Society of London B. 366:619-626

Roberts, N.W., Porter, M. L., Cronin, T.W., 2011 The molecular basis of mechanisms
underlying polarization vision. Philosophical Transactions of the Royal Society of London B
366:627-637

Chiou, T. H., Marshall, N. J., Caldwell, R. L., Cronin, T. W., 2011 Changes in light reflecting
properties of signaling appendages after choice behavior in a stomatopod crustacean,
Haptosquilla trispinosa. Marine and Freshwater Physiology and Behaviour 44:1-11

Chalasani, K., Brewster, R., 2011 N-cadherine-mediated adhesion restricts cell proliferation in
the dorsal neural tube. Molecular Biology of the Cell, 22(9):1505-15 PMC3084673

Blasic, J. R., Brown, R. L., Robinson, P. R., 2012 Phosphorylation of mouse melanospin by
protein kinase A. PIOS. 7(9):e45387. doi: 10.1371

Blasic, J. R., Brown, R. L., Robinson P.R., 2012 Light-dependent phosphorylationof the carboxy
tail of mouse melanopsin. Cell Mol. Life Sci. 69:1551-1562

Matos-Cruz, V., Blasic, J., Robinson, P. R., Hatter, S., Halpern, M., 2011 Unexpected diversity
and photoperiod dependence of the zebrafish melanopsin system. PLOS 6(9): 2511

Porter, M. L., Blasic, J., Bok, M., Cameron, E., Cronin, T. W., Robinson, P. R., 2012 Opsin
Evolution: The big picture. Proc Biol. Sci. 7:279(1726):3-14

Carvalho, L. S., Davies, W. L., Robinson, P. R., Hunt D. M., 2011 Spectral tuning and
evolution of primate short-wavelength-sensitive visual pigments. Proc Biol. Sci. 2012 Jan
22;279(1727):387-93

Acosta, M. A., Velasquez, M., Williams, K., Ross, J. M., Leach, J. B., 2012 Fluorescent silica
particles for monitoring oxygen levels in the three-dimensional heterogeneous cellular structures.
Biotechnology and Bioengineering. DOI: 10.10002/bit.2453

Ribeiro, A., Zustiak, S. P., Vargo, S., Gaifem, H., Hughes, D., Leach, J. B., 2011 Hydrolytically
degradable poly(ethylene glycol)-laminin hydrogel scaffolds for neural progenitor cell delivery.
Society for Biomaterials Abstract #382

Ribeiro, A., Hughes, D., Vargo, S., Voss, E., Powell, E. M., Leach, J. B., 2013 B1-integrin
Cytoskeletal Signaling Regulates Sensory Neurons Response to Matrix Dimensionality.
Neuroscience Sept. 17; 248:67-78

Srpathineni, U., Moss, B., Pollack, J. K., Zhoa, L., Schaefer, D. M., Roberson, R., Marten, M.R.,
2011 Effects of Rapamycin on Filamentous Fungal Cell Walls Biophysical Journal DOI 10.1016

Horn L., Leips, J., Starz-Gaiano, M., 2014 Phagocytic Ability Declines with age in Adult
Drosophilia hemocytes. Aging Cell DOI 10.1111

Venkatsubramanian, A., Drude, A., Good, T., 2014 Role of N-termal residues in AB interactions
with integrin receptor and cell surface Biochem Biophys Acta. DOI 10.1016.

Mendez, M. A., Szalai, V. A., 2013 Synapsable Quadruplex-Mediated Fibers, Nanoscal

Research Letters 2013 8:210 DOI 10.1186/1556-276x-8-210.

Haile, S. T., Dalal, S. P., Clements, V., Tamada, K., Ostrand-Rosenberg, S., 2013 Soluble
CD80 restores T cell activation and overcomes tumor cell programmed death ligand 1-mediated
immune suppression. J. Immunol Sept1; 191(5)2829-36

Leach, L. B., (Invited) Extracellular matrix dimensionality: Culture and evaluation of cellmatrix contracts. In preparation for Neuromethods: Extracellular Matrix, Editors: Jennie B.
Leach and Elizabeth Powell, Publisher: Springer Protocols, Humana Press.

Ribeiro, A., Balasubramanian, S., Hughes, D., Vargo, S., Powell, E., Leach, J. B., 2013 B-1integrin cytoskeletal signaling regulates sensory neuron response to matrix dimensionality.
Neuroscience Sept. 17; 248:67-78 DOI 10.1016

Cronin, T. W., Johnsen, S. K., Marshall, N. J., Warrant, E., 2014 Visual Ecology. Princeton
University Press.

Hamdoun, S., Gill, M., Liu, Z., Yoa, N., Lu, H., 2013 Dynamics of defense response and cell
fate changes during Arabidopsis-Pseudomonas syringae interactions. PLOS DOI 10.1371

Blasic Jr., J. R., Matos-Cuz, V., Ujla, D., Cameron, E., Hattar, S., Robinson, P. R., 2012
Phosphorylation of mouse melanopsin by protein kinase A PLOS DOI10.1371