Kylt Professional in vitro Diagnostic Solutions

Livestock Diagnostic Products

for Poultry
www.kylt.eu

2015

Kylt Livestock Diagnostic Products for Poultry

The Kylt products are designed for rapid and precise
pathogen identification or characterisation in samples derived from livestock. Kylt products are able
to support trustworthy detection of various diseases to
counteract health risk in livestock. Our product range
comprises In Vitro Diagnostics for diverse applications:
detection of bacterial DNA, viral DNA and viral RNA; a
series of bacterial DNA detection kits additionally include the DNA preparation step.

Discover Kylt Livestock Diagnostic Products for Poultry

Our Kylt Livestock Diagnostic Products come in different configurations. The table below gives an overview of
which reagents are included in the product
which basic technique Real-Time vs. conventional
PCR, RT-PCR vs. PCR is used in the product
if additional products are needed to compile a full
set of reagents for the respective testing
if additional protocol steps, such as Agarose Gel
Electrophoresis, are needed to compile the full method.

The information below applies for all articles per product category. Detailed information per individual product categories
are listed in the following pages.

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If you need more information on our Kylt products and categories or you have a specific demand for a product not listed,
please visit us on www.kylt.eu or contact us at info@kylt.eu.

Kylt Real-Time PCR Detection Reagents

Kylt DNA-Extraction & PCR Detection Kits (conventional)

Kylt PCR Detection Kits (conventional)

Kylt Real-Time RT-PCR Detection Reagents

Kylt RT-PCR Detection Kits (conventional)

Kylt RT-PCR Detection Reagents (conventional)

Kylt Real-Time RT-PCR Detection Kits

BCD Enzyme Mixes (please refer to page 8/8)

A
BCD 2x qPCR-Mix
B
BCD 2x RT-qPCR-Mix
C
BCD 2x RT-PCR-Mix

PAGE 2/8

K-C-001, Rev005, August 2015

Kylt Detection Reagents come with all necessary primers (and probes) and controls for
specific detection but without enzyme mixes.

This product includes DNA Extraction-Mix for convenient and simple DNA extraction, but
it may also be used with alternative DNA preparation methods (kits); for all products that
do not include DNA Extraction-Mix separate DNA/RNA preparation methods (kits) are
obligatory
#

Muehlenstr. 13a | 49685 Hoeltinghausen | Germany

Phone + 49 (0) 44 73 94 38 30 | info@kylt.eu | www.kylt.eu

Content
Virus

Page

Bacterium

Page

Parasite

Page

Kylt aCoV (IBV & TuCV)

Kylt APEC (E. coli)

Kylt Avian Encephalomyelitis

Kylt Bordetella avium

Kylt Avian Hepatitis E

Kylt Chlamydiaceae series

Others

Kylt Avian Nephritis Virus

Kylt Clostridium difficile

BCD Enzyme Mixes

Kylt Coryza

Kylt Host Cells

Kylt Avian Rotavirus A&D

Kylt Erysipelothrix

Kylt IC-RNA

Kylt CAV (Chicken Anaemia Virus)

Kylt Mycoplasma series

Kylt Negative Control

Kylt Chicken Astrovirus

Kylt ORT

Kylt Pasteurella multocida

Kylt Riemerella anatipestifer

Kylt Duck Enteritis Virus

Kylt Salmonella spp.

Kylt Duck Hepatitis Virus

Kylt EDS76

Kylt Fowl Adenovirus

Kylt Hemorrhagic Enteritis Virus

Kylt IBDV (Gumboro)

Kylt ILT

Kylt IB Virus series

(Infectious Bronchitis Virus)

Kylt Avian Reovirus

Kylt Duck Baiyangdian Virus

Kylt Duck Circovirus

Kylt Influenza A series

Kylt Reticuloendotheliosis Virus

Kylt TRT series

Kylt Turkey Astrovirus

Kylt Turkey Viral Hepatitis

Kylt Histomonas meleagridis

5
Page

6, 7

Kylt Paramyxovirus 1 (NDV)

German market authorized Kylt Diagnostic Products

In Germany In Vitro Diagnostic Systems for the detection of
notifiable or reportable animal diseases have to be officially
authorised by the Friedrich-Loeffler-Institute (FLI) German
Federal Research Institute For Animal Health before placing
the respective products on the German market. After market
authorisation by FLI every batch of an authorised In Vitro
Diagnostic needs additional release by FLI. A series of authorised and released Kylt Livestock Diagnostic Products received
authorization by FLI:

Muehlenstr. 13a | 49685 Hoeltinghausen | Germany

Phone + 49 (0) 44 73 94 38 30 | info@kylt.eu | www.kylt.eu

 ylt Salmonella spp. DNA Extraction and Real-Time

K
PCR Detection Kit for Salmonella spp. suitable for sock
swabs, feces and dust samples from chickens and turkeys
(approved alternative method to cultural detection based on
EN ISO 6579)
Kylt MGS Triplex DNA Extraction and Real-Time PCR
Detection Kit for Mycoplasma gallisepticum and Mycoplasma synoviae suitable for swab sampling material and pure
and mixed cultural material from chickens and turkeys
Kylt Influenza A Real-Time RT-PCR Detection Kit for
Influenza Virus Type A suitable for swab sampling material,
organs, tissues, saliva- and BALF-samples and sampling material derived from cultural processes from birds and swine

K-C-001, Rev005, August 2015

PAGE 3/8

Kylt DNA Extraction and Real-Time PCR Detection Kits

are for preparation and detection of bacterial DNA of all pathogens listed below. The detection kits contain all reagents and
controls that are necessary for reliable and accurate detection.
Name of Product / Product Category

Because of the harmonized processes of DNA extraction and

Real-Time PCR detection in one kit an optimized performance
is provided.
Real-Time
Quantity of
Reactions

Tested Parameter / Features

Article Number

Kylt DNA Extraction & Real-Time PCR Detection Kits

Kylt Salmonella spp.

Kylt MGS Triplex

Salmonella spp.
(German market authorization FLI-B 656)

100

31019

Mycoplasma gallisepticum / synoviae Triplex

(German market authorization FLI-B 663)

100

31020

25

31021

100

31022

25

31023

100

31024

25

31025

100

31134

25

31135

100

31026

25

31027

100

31028

25

31029

100

31030

25

31031

100

31032

25

31033

for combined detection of Mycoplasma

gallisepticum and/or Mycoplasma synoviae

Mycoplasma gallisepticum

Kylt Mycoplasma gallisepticum

for detection of Mycoplasma gallisepticum

Mycoplasma synoviae

Kylt Mycoplasma synoviae

for detection of Mycoplasma synoviae

Mycoplasma synoviae - DIVA* MS-H

Kylt MS - DIVA* MS-H

for combined detection and differentiation of MS field

strains and vaccine strain MS-H

Kylt Mycoplasma iowae

Mycoplasma iowae

Kylt Mycoplasma meleagridis

Mycoplasma meleagridis

Kylt ORT

Ornithobacterium rhinotracheale

Kylt Riemerella anatipestifer

Riemerella anatipestifer

*DIVA - differentiating infected from vaccinated animals - allows for differentiation of defined vaccine strains from field strains, please refer to product-specific documents
(Validation Report, Direction For Use)

Kylt Real-Time PCR Detection Reagents contain all controls, primers and probes for pathogen-specific detection. An
enzyme mix is not included, but depending on the Kylt ReName of Product / Product Category

agents the matching enzyme mix can be supplied separately,

please refer to page 8/8.
Real-Time
Quantity of
Reactions

Tested Parameter / Features

Article Number

Kylt Real-Time PCR Detection Reagents#

Kylt Chlamydiaceae Screening

Chlamydiaceae Screening

Kylt Chl. psittaci

Chlamydophila psittaci

Kylt Chl. abortus

Chlamydophila abortus

Kylt ILT

Infectious Laryngotracheitis Virus

(Gallid Herpesvirus 1)

100

31036

25

31037

100

31038

25

31039

100

31040

25

31041

100

31042

25

31043

# Kylt Detection Reagents come with all necessary primers (and probes) and controls for specific detection but without enzyme mixes.

PAGE 4/8

K-C-001, Rev005, August 2015

Muehlenstr. 13a | 49685 Hoeltinghausen | Germany

Phone + 49 (0) 44 73 94 38 30 | info@kylt.eu | www.kylt.eu

Kylt DNA Extraction and PCR Detection Kits are for

preparation and detection of bacterial DNA of the pathogens
listed below. The detection kits contain all reagents and controls that are necessary for specific detection of the respective
pathogen. Because of the harmonized processes of DNA exName of Product /
Product Category

traction and PCR detection in one kit an optimized performance

is provided. The subsequent analysis is realized by classical
gel electrophoresis (e.g. agarose gel electrophoresis or capillary gel electrophoresis).
Gel
Quantity of
Reactions

Tested Parameter / Features

Article Number

Kylt DNA Extraction & PCR Detection Kits (conventional)

Kylt APEC

Avian Pathogenic Escherichia Coli 8 x Virulence Factors

Kylt Coryza

Avibacterium paragallinarum (Infectious Coryza)

Kylt Bordetella avium

Bordetella avium

Kylt Clostridium difficile

Clostridium difficile Toxin Genes (A & B)

Kylt Erysipelothrix

Erysipelothrix spp. (Genus-specific) &

E. rhusiopathiae (Species-specific)

Kylt Pasteurella multocida

Pasteurella multocida (Species-specific)

Kylt PCR Detection Kits contain all reagents, controls and

primers for pathogen-specific detection. The subsequent ana
Name of Product /
Product Category

100

31046

25

31047

100

31048

25

31049

100

31050

25

31051

100

31052

25

31053

100

31054

25

31055

100

31058

25

31059

lysis is realized by classical gel electrophoresis (e.g. agarose

gel electrophoresis or capillary gel electrophoresis).
Gel

Quantity of
Reactions

Tested Parameter / Features

Article Number

Kylt PCR Detection Kits (conventional)

Kylt Histomonas meleagridis

Histomonas meleagridis

Kylt CAV

Chicken Anaemia Virus (Circoviridae)

Kylt Duck Circovirus

Duck Circovirus & Goose Circovirus (Species-specific)

Kylt EDS76

Egg Drop Syndrome Virus (EDS76, 127-like, Duck Adenovirus)

Kylt Hemorrhagic Enteritis Virus Turkey Hemorrhagic Enteritis Virus (Adenoviridae)

Kylt Duck Enteritis Virus

Duck Enteritis virus (Anatid Herpesvirus 1)

Kylt Reticuloendotheliosis Virus Reticuloendotheliosis Virus

Kylt Fowl Adenovirus

Fowl Adenovirus (Group I)

Muehlenstr. 13a | 49685 Hoeltinghausen | Germany

Phone + 49 (0) 44 73 94 38 30 | info@kylt.eu | www.kylt.eu

K-C-001, Rev005, August 2015

100

31056

25

31057

100

31060

25

31061

100

31062

25

31063

100

31064

25

31065

100

31066

25

31067

100

31140

25

31141

100

31142

25

31143

100

31144

25

31145

PAGE 5/8

Kylt Real-Time RT-PCR Detection Kits are for detection

of viral RNA of the pathogens listed below. The detection kits
contain all reagents and controls that are necessary for the
Name of Product / Product Category

process of Reverse Transcription (RT) followed by Real-Time

PCR and allow for a reliable and accurate detection of the
pathogen.
Quantity of
Reactions

Tested Parameter / Features

Real-Time

Article Number

Kylt Real-Time RT-PCR Detection Kits

Kylt Influenza A

Kylt Influenza A - H9

Influenza Virus Type A

(German market authorization FLI-B 672)

100

31068

25

31069

100

31070

25

31071

for further differentiation see Kylt IB Virus below

100
25
100
25
100
25
100
25

31072
31073
31074
31075
31076
31077
31078
31079

Infectious Bronchitis Virus

100

31080

25
100
25
100
25
100
25
100
25
100
25
100
25
100
25
100
25
100
25
100
25
100
25
100
25

31081
31082
31083
31084
31085
31086
31087
31088
31089
31090
31091
31092
31093
31094
31095
31098
31099
31100
31101
31102
31103
31104
31105
31106
31107

for Influenza A serotype differentiation see our Kylt Influenza A - Serotype Detection Reagents below and page 7/8

Avian Influenza Virus H9 (H9-type-specific)

for previous Influenza Virus Type A screening
see Kylt Influenza A above

Kylt aMPV A&B

Avian Metapneumovirus Subtype A & B (TRT, ART)

Kylt aMPV C

Avian Metapneumovirus Subtype C (TRT, ART)

Kylt aMPV D

Avian Metapneumovirus Subtype D (TRT, ART)

Kylt aCoV (IBV & TuCV)

Kylt IB Virus
Kylt IBV-Variant 4/91 (793b)
Kylt IBV-Variant Massachusetts
Kylt IBV-Variant D274
Kylt IBV-Variant Arkansas
Kylt IBV-Variant Italy02
Kylt IBV-Variant D1466
Kylt IBV-Variant QX

Avian Coronavirus Screening (including IBV & TuCV)

for IBV variant differentiation see our Kylt
IBV-Variant Real-Time RT-PCR Detection Kits below

Infectious Bronchitis Virus - Variant 4/91 (793b)

for previous IBV screening see Kylt IB Virus above

Infectious Bronchitis Virus - Variant Massachusetts

for previous IBV screening see Kylt IB Virus above

Infectious Bronchitis Virus - Variant D274

for previous IBV screening see Kylt IB Virus above

Infectious Bronchitis Virus - Variant Arkansas

for previous IBV screening see Kylt IB Virus above

Infectious Bronchitis Virus - Variant Italy02

for previous IBV screening see Kylt IB Virus above

Infectious Bronchitis Virus - Variant D1466

for previous IBV screening see Kylt IB Virus above

Infectious Bronchitis Virus - Variant QX

for previous IBV screening see Kylt IB Virus above

Kylt Avian Nephritis Virus

Avian Nephritis Virus (Astrovirus)

Kylt Avian Hepatitis E

Avian Hepatitis E Virus (aHEV, Hepevirus, BLSD,

Big Liver and Spleen Disease Virus)

Kylt Turkey Viral Hepatitis

Turkey Viral Hepatitis Virus (Picornaviridae)

Kylt Avian Rotavirus A&D

Avian Rotavirus Group A & D (Group-specific)

Kylt Host Cells

Host Cells (Detection of beta-actin mRNA, Control

Reaction Kit)

PAGE 6/8

K-C-001, Rev005, August 2015

Muehlenstr. 13a | 49685 Hoeltinghausen | Germany

Phone + 49 (0) 44 73 94 38 30 | info@kylt.eu | www.kylt.eu

Kylt Real-Time RT-PCR Detection Reagents contain all

controls, primers and probes for pathogen-specific detection.
An enzyme mix is not included, but depending on the Kylt
Name of Product / Product Category

Reagents the matching enzyme mix can be supplied separately, please refer to page 8/8.

Quantity of
Reactions

Tested Parameter / Features

Real-Time
Article Number

Kylt Real-Time RT-PCR Detection Reagents#

Avian Influenza Virus H5 (H5-type-specific)

Kylt Influenza A - H5

100

31108

25

31109

100

31110

25

31111

Avian Influenza Virus N1 (N1-type-specific)

100

31112

25

31113

Avian Paramyxovirus Type 1 (including Newcastle

Disease Virus, ND)

100
25

31114
31115

for previous Influenza Virus Type A screening

see Kylt Influenza A, see page 6/8

Avian Influenza Virus H7 (H7-type-specific)

Kylt Influenza A - H7

for previous Influenza Virus Type A screening

see Kylt Influenza A, see page 6/8

Kylt Influenza A - N1

for previous Influenza Virus Type A screening

see Kylt Influenza A, see page 6/8

Kylt Paramyxovirus 1

# Kylt Detection Reagents come with all necessary primers (and probes) and controls for specific detection but without enzyme mixes.

Kylt RT-PCR Detection Kits are for detection of viral RNA

of the pathogens listed below. The detection kits contain all
reagents and controls that are necessary for the process of

Reverse Transcription (RT) followed by PCR. The subsequent

analysis is realized by classical gel electrophoresis (e.g. agarose gel electrophoresis or capillary gel electrophoresis).
Gel

Name of Product / Product Category

Quantity of
Reactions

Tested Parameter / Features

Article Number

Kylt RT-PCR Detection Kits (conventional)

Kylt Avian Encephalomyelitis

Avian Encephalomyelitis-like Virus 1

(Hepatovirus, Pircornaviridae)

Kylt Duck Baiyangdian Virus

Duck Baiyangdian Virus (BYDV, Flaviviridae)

Kylt Duck Hepatitis Virus

Duck Hepatitis Virus Type I (DHV-I, Picornaviridae)

Kylt Chicken Astrovirus

Chicken Astrovirus

Kylt Turkey Astrovirus

Turkey Astrovirus (Differentiation of type 1 and

type 2)

Kylt Avian Reovirus

Avian Orthoreovirus

Kylt RT-PCR Detection Reagents contain all controls and

primers for pathogen-specific detection. An enzyme mix is not
Name of Product / Product Category

100
25
100
25
100
25
100
25
100
25
100
25

31118
31119
31120
31121
31122
31123
31124
31125
31136
31137
31146
31147

included, but depending on the Kylt Reagents the matching enzyme mix can be supplied separately, please refer to page 8/8.
Quantity of
Reactions

Tested Parameter / Features

Gel
Article Number

Kylt RT-PCR Detection Reagents# (conventional)

Kylt IBDV

Infectious Bursal Disease Virus (Gumboro)

100

31126

25

31127

# Kylt Detection Reagents come with all necessary primers (and probes) and controls for specific detection but without enzyme mixes.

Muehlenstr. 13a | 49685 Hoeltinghausen | Germany

Phone + 49 (0) 44 73 94 38 30 | info@kylt.eu | www.kylt.eu

K-C-001, Rev005, August 2015

PAGE 7/8

BCD Enzyme Mixes are suitable to be combined with respective Kylt Detection
Reagents to assemble the complete (Real-Time) (RT-)PCR method.
Name of Product / Product Category

Quantity of
Reactions

Tested Parameter / Features

Article Number

BCD Enzyme Mixes

BCD 2x qPCR-Mix

2 fold concentrated Real-Time PCR-Mix

100

31129

BCD 2x PCR-Mix

2 fold concentrated conventional PCR-Mix

100

31131

BCD 2x RT-qPCR-Mix

2 fold concentrated Real-Time RT-PCR-Mix

100

31128

BCD 2x RT-PCR-Mix

2 fold concentrated conventional RT-PCR-Mix

100

31130

Name of Product / Product Category

Tested Parameter / Features

Quantity of
Reactions

Article Number

Kylt Real-Time PCR Controls

Kylt IC-RNA

Internal Control RNA

100

31132

Kylt Negative Control

Negative Control

1 ml

31133

The Kylt Real-Time (RT-)PCR products provide you the

following advantages:
You can run them in the same thermal profile for all standardly monitored pathogens.
You can run them on virtually any Real-Time PCR thermal
cycler.
They allow economic pooling of individual samples (in contrast to serology).
They proved to be highly satisfactory and reliable in our daily
high-throughput routine diagnostic services for samples from
all over the world.
They are fully validated according to applicable German
FLI-guidelines, ISO 16140 etc. to meet all potential regulatory
requirements by your authorities.
They have received market authorization in Germany by
FLI (this currently applies to Kylt Salmonella spp., Kylt
Influenza A and Kylt MGS Triplex).

The users of our Kylt Real-Time (RT-)PCR products

benefit from
direct support by your local technical and academic contact
partner
background support by our experts in Germany
a choice of different hands-on training options at your site,
at the local distributors site or at AniCons training center in
Germany
our Kylt Software package for easy entry of sample data,
PCR run, analysis and reporting of results, which is provided
to our customers free of charge and may be customized to
their individual equipment, QC-requirements and linkage to
LIMS, if applicable
regular updates of individual kits for inclusivity of all emerging strains worldwide
ongoing extension of the product catalogue with respect to
upcoming diagnostic needs.

For veterinary use only. For in vitro use only. Regulatory requirements vary by country, these products may not be available in your geographic area.
2015 AniCon Labor GmbH. All rights reserved. The trademark mentioned herein is the property of AniCon Labor GmbH or their respective owners.
THE PURCHASE OF THIS PRODUCT GRANTS THE PURCHASER RIGHTS UNDER CERTAIN ROCHE PATENTS TO USE IT FOR PROVIDING VETERINARY IN VITRO DIAGNOSTIC TESTING. NO GENERAL
PATENT OR OTHER LICENSE OF ANY KIND OTHER THAN THIS SPECIFIC RIGHT OF USE FROM PURCHASE IS GRANTED HEREBY.

PAGE 8/8

K-C-001, Rev005, August 2015

Muehlenstr. 13a | 49685 Hoeltinghausen | Germany

Phone + 49 (0) 44 73 94 38 30 | info@kylt.eu | www.kylt.eu