May | June 2010

Feature title: LiptoCitro - Phytobiotics and Prebiotics: a new alternative for

sustainable aquaculturePhytobiotics and Prebiotics
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The International magazine for the aquaculture feed industry

 F: LiptoCitro F: LiptoCitro

Table 2: Effect of prebiotic LiptoCitro on Daily Intake Rate (DIR), Feed

Conversion Ratio (FCR) and Survival Rate in sea bass (Dicentrarchus labrax).
Data from six month laboratory trial using four replicates (eight tanks total)
with 100 fish per tank. *Dosages of 3g/Kg. Values shown are the mean ±
standard error

Feed % Effect


Without Feed With (LiptoCitro
Significance
LiptoCitro LiptoCitro* vs.
(Control) Control)

DIR 1.28 ± 0.04 1.19 ± 0.04  7.03% p<0.05


Phytobiotics and Prebiotics
FCR 1.38 ± 0.05 1.27 ± 0.04  7.97% p<0.05

Survival Rate 94.75 ± 6.13 98.00 ± 1.41  3.25% n.s.

 

A new alternative for sustainable aquaculture recovered and did not need any antibiotic ment of the growth

by Santiago de la Cuesta, Ignacio López, Antonio Martínez and Laura Muñoz, Spain
treatment at any stage of the trials.
Trials conducted throughout a production
and survival rates.
LiptoCitro anti-

Email: liptosa@liptosa.com, Website: www.liptosa.com
cycle on rainbow trout (Oncorhynchus mykiss), bacterial efficiency,
under commercial conditions and using con- survival and FCR

E
trol lots, showed 14 percent improvement in improvement and
nvironmentally friendly LiptoCitro trial data the lots of infected fish that were fed FCR in the fish that were fed with 2.5 Kg/MT growth and immune
LiptoCitro is a prebi- Trials have been conducted in laborato- with LiptoCitro improved significantly, of LiptoCitro. This digestibility improvement system promoter
otic, immunoestimulant and ries, research centres and farms was evaluated in further trials using the same effect have also
growth promoter with a with a range of fish and shrimp Table 1: Minimum Inhibitory Concentration (MIC) of inclusion rates of Liptocitro in the feed. The been tested in vari-
LiptoCitro against bacterial strains tested in VISAVET
proved antibacterial activity. It can be species and pathogens. results showed that the cost of the ration ous shrimp species
laboratory, Spain
used at any stage of the fish produc- Trials conducted in the could be reduced without altering FCR. This and under different
tion cycle and no withdrawal period Veterinary Sanitary Vigilance Microorganism MIC was achieved by decreasing the amount of farming and envi-
is needed because it does not contain Centre (VISAVET) of the expensive animal origin protein in the diet and ronmental condi-
any antibiotics. It is used at dosage Complutense University of Aeromonas hydrophila 3 mg/ml increasing the inclusion of cheaper vegetable tions. These results
rates of 2.5Kg/M/tonne and it can be Madrid, Spain showed that Aeromonas salmonicida subsp. salmonicida 1 mg/ml protein instead. can be provided
incorporated in the feed mill during LiptoCitro possessed strong in Six month trials were conducted in four upon request.
Listonella anguillarum 6 mg/ml
diet manufacture, or top-dressed on the vitro antibacterial activity against replicate tanks in the laboratories of the Trials carried
Listonella anguillarum CECT 522 3 mg/ml
feed using oil or molasses at the farm. a range of pathogenic bacterial Agricultural Development and Food Research out with LiptoCitro
species commonly encountered in Leucothrix mucor DSM 2157T 1 mg/ml Institute IMIDA, in Murcia, Spain. These have shown that the
It is made of a combination of prebiotics, aquaculture production systems Vibrio penaeicida DSM 14398T 3 mg/ml trials were carried on European sea bass use of prebiotics
plant extracts and plant essences and does (Table 1). Vibrio harveyi CECT 525T 1 mg/ml (Dicentrarchus labrax) under ideal conditions and phytobiotics in
not develop off-flavours in either fish or Trials carried from May to and without a disease challenge. The fish fish and shrimp feed
Vibrio parahaemolyticus 6 mg/ml
crustaceans. LiptoCitro is capable of improv- November in a commer- fed three g/kg of Liptocitro showed three leads into better
Vibrio ordali 6 mg/ml
ing nutrient digestibility, favouring beneficial cial farm in Spain showed the percent improvement in growth and survival production param-
intestinal flora, eliminating pathogenic bacteria, efficacy of Liptocitro to con- Vibrio vulnificus 1 mg/ml rates and eight percent improvement in FCR eters and enables
and promoting the immune system, resulting trol Haemorrhagic Enteritis Vibrio vulnificus CECT 529 0.1 mg/ml over the fish in the control tanks (statisti- sustainable aquacul-
in higher resistance to stress and disease and outbreaks in rainbow trout Vibrio alginolyticus 6 mg/ml cally significant at 95 percent confidence level) ture production on
improved growth, survival, FCR, production (Oncorhynchus mykiss). A total Flavobacterium psycrophilum DSMZ 3660T 3 mg/ml
(Table 2). Improvements in the haemocrit and a long term basis
and cost-efficiency, even in the event of bacte- amount of 1,700 MT of commer- haemoglobin levels of fish blood following a by reducing the use
Edwarsiella ictaluri CECT 885T 13 mg/ml

rial, viral and some parasitic disease outbreaks. cial feed with LiptoCitro added
at 2.5Kg/MT was
used. All the fish had
the same handling
7,97% and usual European
vaccine prophylaxis.
In the control lots,
Photobacterium damselae subsp. piscicida
Photobacterium damselae subsp. damselae
which were not fed
Liptocitro, several
Enteritis outbreaks
Brevundimonas diminuta CECT 317T
Pseudomonas anguilliseptica
Pseudomonas fluorescens
Yersinia ruckeri
Lactococcus garvieae


stress test were also observed. of antibiotics and
1 mg/ml These trials have shown how a better decreasing fish meal
0.1 mg/ml intestinal integrity, even under ideal conditions content in diets.
3 mg/ml of low density and very low mortality, results References
3 mg/ml
3 mg/ml
in better nutrient assimilation thus improving
the feed conversion rate. Therefore, in the
field, a better nutrient
are available upon
request. 
 
­€€€€
6 mg/ml assimilation as a result
6 mg/ml of a good intestinal More infoirMation:
integrity will have


Streptococcus iniae CCUG 27303T 13 mg/ml Antonio Martinez
occurred over the Streptococcus difficilis DSZM 16828 13 mg/ml even more advanta- Lipidos Toledo SA, Spain
six month trial peri- geous effects than Tel: +34 902157711Fax: +34 913567300
Streptococcus parauberis 6 mg/ml Email: antonio.m.sanchez@liptosa.com  
od and the fish had those observed under
Mycobacterium chelonae CCUG 47445T 13 mg/ml Web: www.liptosa.com
to be treated with ideal conditions with
 

antibiotics. However, Mycobacterium marinum 0.1 mg/ml regards the improve-

24 | InternatIonal AquAFeed | May-June 2010 May-June 2010 | InternatIonal AquAFeed | 25

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Phytobiotics and
Prebiotics:
- a new alternative for sustainable
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Innovative approaches
to reduce feed cost in
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- Optimizing nutrient utilization and gut health
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