Beruflich Dokumente
Kultur Dokumente
LPIDOS
1. cidos grasos
2. Triacilgliceroles
3. steres de ceras
4. Fosfolpidos
5. Esfingolpidos
6. Isoprenoides
cidos Grasos
CH3(CH2)14-COONo-polar
polar
CABEZA
Ionizado en condiciones
fisiolgicas, generalmente
esterificado
COLA
14 24 C
cidos grasos
SATURADOS E INSATURADOS
MONOINSATURADO
cidos Grasos
cido Hexadecanoico
16:0
cido cis-9-Octadecenoico
18:1(D9 )
cido oleico
18:1(D9 )
Tf = 13.4 oC
metilo terminal
3
2
C
1
Triacilgliceroles:
steres de glicerol
Fatty
acid
ESTERIFICACIN
Sntesis de
Formation
of un
an ster
ester:
O
R'OH + HO-C-R"
O
R'-O-C-R'' + H2O
ESTERIFICACIN LPIDO
=O
H2O
Saponificacin
O
O CH2 OCR
saponification
+
3
N
aOH
RCOCH
O
CH2 OCR
A triglyceride
( a triester of glycerol)
CH2 OH
CHOH
O
+
3 RCO N a
CH2 OH
1,2,3-Propanetriol S odium soaps
(Glycerol; glycerin)
steres de ceras
Fosfolpidos
Componente importante
de la membrana mitocondrial interna
(lecitina)
(cefalina)
Glicerofosfolipido antignico
Esfingolpidos
ceramida
fosforilcolina
Isoprenoides
Isopreno
Terpenos y Esteroides
Esteroides
Esteroides
Esteroides
Terpenos
Terpenos
Bilayer
Spherical Micelle
Membrane fluidity:
The interior of a lipid bilayer is
normally highly fluid.
liquid crystal
crystal
HO
Cholesterol
Cholesterol
in membrane
Fluidez de la Membrana
Interaction with the relatively rigid cholesterol
decreases the mobility of hydrocarbon tails of
phospholipids.
Cholesterol
in membrane
Membranas
Lateral mobility of a lipid, within the plane of a
membrane.
High speed tracking of individual lipid
molecules has shown that lateral movements
are constrained within small membrane
domains.
Hopping from one domain to another occurs
less frequently than rapid movements within a
domain.
The apparent constraints on lateral
movements of lipids (and proteins) has been
attributed to integral membrane proteins,
anchored to the cytoskeleton, functioning as a
picket fence.
Lateral Mobility
Flip Flop
peripheral
Membrane proteins
may be classified as:
peripheral
integral
having a lipid
anchor
lipid
anchor
lipid bilayer
integral
Membrane
Proteins
lipid
anchor
O
H 3C
membrane
(CH2)14
palmitate
O
S
CH2
C
CH
cysteine
residue
NH
CH3
CH3
H3C
CH
CH2
CH2
CH3
CH
CH2
CH2 C
CH
CH2
Protein
lipid
anchor
membrane
peripheral
lipid
anchor
lipid bilayer
integral
Membrane
Proteins
membrane
Amphipathic detergents
are required for
solubilization of integral
proteins from membranes.
detergent
solubilization
polar
non-polar
Protein with
bound detergent
Balsas lipdicas:
Complex sphingolipids tend to separate out from
glycerophospholipids & co-localize with cholesterol in
membrane microdomains called lipid rafts.
Membrane fragments assumed to be lipid rafts are
found to be resistant to detergent solubilization,
which has facilitated their isolation & characterization.
CH3
H3 C
O
H2
C
H2
C
CH3
phosphocholine
H2C
sphingosine
OH
H
C
CH
NH
CH
HO
Sphingomyelin
fatty acid
HC
Cholesterol
(CH2 )12
CH3
caveolae
cytosol