Principal Investigator/Program Director (Last, first, middle):

BIOGRAPHICAL SKETCH

NAME POSITION
Professor of Experimental Pathology & Head of
Terence A. Partridge
Muscle Cell Biology Group
EDUCATION/TRAINING
DEGREE
INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY
(if applicable)

University College London, London, UK B.Sc. 1962 Zoology

University College London, London, UK Ph.D. 1970 Zoology
(Embryology)
A. Positions and Honors
POSITIONS AND EMPLOYMENT
1965 – 1966 Research Assistant – Museum Nationale d’Histoire Naturelle, Paris
1967 – 1970 Lecturer in Cell Biology – University of Glasgow
1970 – 1975 Research Fellow - Department of Experimental Pathology, Charing Cross Hospital
Medical School,London.
1975 – 1980 Lecturer, Department of Experimental Pathology, Charing Cross Hospital Medical School,
London
1978 – 1989 Senior Lecturer, Department of Experimental Pathology, Charing Cross Hospital Medical
School, London
1989 – 1992 Reader in Experimental Pathology & Head of Muscle Cell Biology Group, MRC Clinical
Sciences Centre, Imperial College School of Medicine
1993 – 1994 Professor of Experimental Pathology & Head of Muscle Cell Biology Group, MRC Clinical
Sciences Centre, Imperial College School of Medicine
1994 – Present Professor of Experimental Pathology & Head of Muscle Cell Biology Group, MRC Clinical
Sciences Centre, Imperial College School of Medicine,
HONORS
2002 - 2003 Member of National Center for Research Resources (NCRR), the National Institutes of
Health (NIH). NCRR Human Embryonic Stem Ccll Special Emphasis Panel
2002 Elected to Fellowship of Academy of Medical Sciences
2004 - 2006 Chair Blaise Pascal - Fellowship awarded by the Region of the Ile de France

B. Selected peer-reviewed publications (in chronological order). (Of 128 publications).

Blaveri, K., Heslop, L., Yu, D.S., Rosenblatt, J.D., Gross, J.G. Partridge, T.A. & Morgan, J.E. (2000)
Patterns of repair of dystrophic mouse muscle: studies on isolated fibres. Developmental Dynamics,
216, 244-256.
Lu, Q.L., Morris, G.E., Wilton, S.D., Ly, T., Artem’yeva, O.V., Strong, P., Partridge, T.A. (2000) Massive
idiosyncratic exon skipping corrects the nonsense mutation in dystrophic mouse muscle and
produces functional revertant fibres by clonal expansion. J. Cell Biol. 148,985-995.
Heslop, L. Morgan, J.E. and Partridge, T.A. (2000) Evidence for a myogenic stem cell that is exhausted in
dystrophic muscle. J. Cell Science 113, 2299-2308.
Morrison, J., Lu, Q.L., Pastoret, C., Partridge, T., Bou-Gharios, G. (2000) T-cell-dependent fibrosis in the
mdx dystrophic mouse. Lab.Invest. 80, 881-891.
Pagel, C.N., Morgan, J.E., Gross, J.G., Partridge, T.A. (2000). Thymic myoid cells as a source of cells for
myoblast transfer. Cell Transplantation, 9: 531-538.
Cooper, R. N.; Irintchev, A.; Di Santo, J. P.; Zweyer, M.; Morgan, J. E.; Partridge, T. A.; Butler-Browne, G.
S.; Mouly, V., Wernig, A. (2001) A new immunodeficient mouse model for human myoblast
transplantation. Human Gene Therapy; 12: 823-831.
Beauchamp, J.R., Heslop, L., Yu, D.S.W., Tajbakhsh, S., Kelly, R.G.‡ Buckingham, M.E. Partridge, T.A.,
Zammit P.S. (2000) Expression of CD34 and Myf5 defines the majority of quiescent adult skeletal
muscle satellite cells. J. Cell Biology 151: 1221-1233.
Mann, C.J., Honeyman, K., Chen, A.J., Ly, T., Lloyd, F., Fletcher, S., Morgan, J.E., Partridge, T.A., Wilton,
S.D. (2001). Antisense-induced exon skipping and synthesis of dystrophin in the mdx mouse.
Proceedings of the National Academy of Sciences, U.S.A. 98: 42-47.

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 Principal Investigator/Program Director (Last, first, middle):

Heslop, L., Beauchamp, J.R., Tajbakhsh, S., Buckingham, M.E., Partridge, T.A., Zammit, P.S. (2001)
Transplanted primary myoblasts can give rise to functional satellite cells as identified using the
Myf5nlacZ/+ mouse. Human Gene Therapy, 8: 778-783.
Shefer, G., Partridge, T.A., Heslop, L., Gross, J.G., Oron, U. Halevy, O. (2002) Low-energy laser irradiation
promotes the survival and cell-cycle entry of skeletal muscle satellite cells. Journal of Cell Science,
115: 1461-1469.
De Val, S., Ponticos, M., Antoniv, T.T., Wells, D.J., Abraham, D., Partridge, T., Bou-Gharios, G. (2002).
Indentification of the key regions within the mouse pro- 2(I) Collagen gene far upstream enhancer.
Journal of Biological Chemistry, 277: 9286-9292.
Morgan, J.E., Pagel, C.N., Beauchamp, J.R., Gross, J.G., Fassati, A., . Thrasher, A.J., Di Santo, J.P.,
Abraham, D.J., Fisher, I.B, Shiwen, X., Partridge T.A. (2002). Myogenic cell proliferation and
generation of a reversible tumorogenic phenotype are triggered by pre-irradiation of the recipient site.
Journal of Cell Biology.157:693-702.
Zammit, P. S.; Heslop, L.; Hudon, V.; Rosenblatt, J. D.; Tajbakhsh, S.; Buckingham, M. E.; Beauchamp, J.
R., and Partridge, T. A. (2002) Kinetics of myoblast proliferation show that resident satellite cells are
competent to fully regenerate skeletal muscle fibers. Experimental Cell Research 15; 281: 39-49.
Wells, K. E.; Torelli, S.; Lu, Q.; Brown, S. C.; Partridge, T.; Muntoni, F., and Wells, D. J. (2003)
Relocalization of neuronal nitric oxide synthase (nNOS) as a marker for complete restoration of the
dystrophin associated protein complex in skeletal muscle. Neuromuscular Disorders. 13: 21-31.
Lu, Q-L, Bou-Gharios, G, Partridge, TA, (2003) Non-viral gene delivery in skeletal muscle: a protein factory.
Gene Therapy.; 10:131-142
Lu, Q-L, Liang, H-D, Partridge, T, Blomley, MJK (2003) Microbubble ultrasound improves the efficiency of
gene transduction in skeletal muscle in vivo with reduced tissue damage. Gene Therapy. 10: 396-
405
Lu, Q-L., Mann C. J., Lou, F., Bou-Gharios, G., Morris, G. E., Xue, S., Fletcher, S., Partridge, T. A. &
Wilton, S. D. Functional amounts of dystrophin produced by skipping the mutated exon in the mdx
dystrophic mouse. Nature Medicine 9, 1009 – 1014
ReimannJ, Brimah K, Schröder R, Wernig A, Beauchamp JR, Partridge TA. (2004). Pax7 distribution in
human skeletal muscle biopsies and myogenic tissue cultures. Cell Tissue Res. 315;233-243
Murtuza, B, Suzuki, K., Bou-Gharios, G., Beauchamp J. R., Smolenski , R. T., Partridge, T. A., Yacoub M.
H. Transplantation of myogenic precursors overexpressing interleukin-1 receptor antagonist
modulates adverse remodeling in infarcted murine myocardium: PNAS:101;4216–4221
Cousins J.C., Woodward K. J.,. Gross J.G .,. Partridge T. A., Morgan. J.E. Regeneration of skeletal muscle
from transplanted immortalised myoblasts is oligoclonal. J. Cell Sci. in press.
Ponticos, M., Abraham, D., Alexakis, C Lu, ., Q-L., Black C,, Partridge T. Bou-Gharios G. Col1a2 enhancer
regulates collagen activity during development and in adult tissue repair. Matrix Biology. (In press)
Ponticos, M., Partridge T., Black1, C. M.. Abraham D. J., Bou-Gharios G. Regulation of collagen type I in
vascular smooth muscle cells by competition between Nkx2.5 and deltaEF1/ZEB1. Mol Cell Biol, (in
press)
Zammit, P.S. ,Golding, J.P., Nagata, Y., Hudon, V., Partridge, T.A., Beauchamp, J.R. (2004) Muscle
satellite cells adopt divergent fates: a mechanism for self-renewal? Journal of Cell Biology (in press).
Peter S. Zammit†, Jaime J. Carvajal*, Jon P. Golding, Jennifer E. Morgan, Dennis Summerbell*, Terence
A. Partridge, Peter W. J. Rigby* and Jonathan R. Beauchamp Myf5 expression in satellite cells and
muscle spindles of adult muscle is controlled by separate genetic elements. Developmental Biology,
(in press)

C. Research Support

ONGOING RESEARCH:

Program Grant from 2000- 2005 Cell Biology of Skeletal

MRC muscle

The objective of this program is to integrate studies of cellular functions in the maintenance and
repair of skeletal muscle in vivo and in vitro so as to generate a coherent picture of the mechanims
involved.
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 Principal Investigator/Program Director (Last, first, middle):

RESEARCH COMPLETED DURING THE LAST 3 YEARS:

European Community 1999-2002 Stem cells and precursor cells

Network Grant of skeletal muscle
Framework 5

This funding was specifically aimed at generating collaborative networks with other European
laboratories so as to facilitate collaborations on the study of myogenic stem cells.

Muscular Dystrophy 2001-4 Development and

Association characterization of new
markers for satellite cells

This MDA funded project was aimed at finding new reagents to permit the identification and
characterization of the skeletal muscle satellite cell. We have identified a number of new markers
and have used them to demonstrate the behavioural heterogeneity of satellite cells.

Aktion Benni & Co 2002-4 Endogenous mechanisms of

fibrosis in mdx mouse muscle

This project is aimed at determining which cells in skeletal muscle are responsible for the fibrosis
that occurs in chronically injured muscle and the control elements of the collagen 1 gene that are
responsible for the excessive production of scar tissue in such muscles.

European Community 1999-2003 Effects of aging on skeletal

Network Grant - muscle satellite cells

This European funding was directed at fostering cooperation between a number of laboratories
working on various aspects of aging in human and mouse skeletal muscle

Muscular Dystrophy 2004 - 2005 Characterization of the

Campaign. (with J. endogenous satellite stem cell
Morgan & P.Zammit subpopulation

This is one year of funding to establish a new technique for analyzing myogenicity of satellite cells
by transplanting them in very small numbers (5-10) on single isolated muscle fibres. We find that
under these conditions, satellite cells behave in a more ‘stem cell-like’ way than when they are
separated from the muscle fibre

Association Francaise 2004 - 2005 Characterization of the

contre les Myopathies myogenic properties of the
satellite cell

This funding is specifically to foster the use of the single fibre transplantation technique for analysis
of myogenic cell behaviour using a number of transgenic markers that are available in mouse
colonies in laboratories at the Genethon and the Pasteur Institute.

Chair Internationale de 2004 - 2006 Investigation of the diversity of

Recherche Blaise satellite phenotypes and
Pascal de l’Etat et de la functions
Region d’Ile-de-France,

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 Principal Investigator/Program Director (Last, first, middle):

This is a prestigious award of financial support to conduct work of collaboration for a total duration
of 1 year spread over a 2 year period. It is envisaged as involving the best Parisian laboratories
(Genethon and Institut Pasteur) and the home laboratory ( in this case my MRC laboratory during
2005 and the Children’s Hospital during 2006. The objective is to bring together the specialist
techniques of both sets of laboratories to gain previously unobtainable information on the nature of
the variety of cells that are grouped together under the name of satellite cell and to determine the
roles of these cell types in muscle repair after various types of injury.

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