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Appendix B: Example for calculating IUPAC-FPU
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The practical bottom line is that if the assays are set up according to the instructions, and the
calculations are carried o u t using the equation presented in the calculations section, the results
obtained will correspond to the generally accepted activities in " filt er paper units" that wo u ld be
obtained by other laboratories around the wo rld , were these other laboratories to test the same
enzyme solution. For those workers interested in the derivation of this equation, and of the " filt er
paper u n it " , the fo llo win g comments may be h elp fu l in conjunction wit h Ghose (1987).
The numerator (0.37) in the equation is derived fro m the factor fo r converting the 2.0 mg of
"glucose-equivalents" generated in the assay to mmoles of glucose (2.0 0.18016), fro m the volume
of the enzyme being tested that is used in the assay (0.5 mL), and fro m the incubation time (60
minutes) required fo r generation of the reducing equivalents.
Procedure #006
Revision: 7/18/96
Supersedes: 8/19/92
Page 8 of 11