Beruflich Dokumente
Kultur Dokumente
Received: Apr 21, 2016; Accepted: May 13, 2016; Published: May 23, 2016; Paper Id.: IJASRJUN2016042
INTRODUCTION
To meet the potential challenge of catering to the food need of 1.4 billion people of our country by 2025,
Original Article
a quantum increase in agricultural productivity is very much essential, hence production and distribution of high
quality seeds of improved varieties of cereals, pulses, oil seeds and vegetables to the farming community is
becoming increasingly important. Vegetables constitute a major part in Indian agriculture in terms of providing
food and nutritional security. In recent past, Indian agriculture has witnessed a tremendous progress in vegetable
production, due to the advent of high yielding varieties and new technologies. Vegetables are important sources of
minerals, vitamins and other nutrients of medicinal and therapeutic value. Regular consumption of vegetables is a
must for the nutritional security of the household. The production and productivity of different vegetable crops
have been increased significantly in the past twenty years as a result of research and development pursuits
undertaken by agricultural institutions as well as private sectors. Modern strategic approaches are however
necessary for sustainable development of these crops so as to meet increasing requirement in both domestic and
export market.
Among vegetable crops solanaceous vegetables like tomato, brinjal and chilli have high demand in our
state as well as in the country. Tomato (Solanum lycopersicum L.) is one of the most important edible and
nutritious vegetable crop in the world. It ranks next to potato and sweet potato with respect to world vegetable
production. It ranks third in terms of world vegetable production. The demand for the crop is year round, owing to
the versatility of its usages both in fresh and processed food preparation. It gains more popularity due to its
antioxidant property. It is a very good source of vitamin A and C.
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medium grade perlite and placed in greenhouse for 40 days. Pots were daily irrigated with nutrient solutions containing
different levels of salinity (0.2, 5, 10, 15 and 20 dSm-1). Results showed that germination percentage of primed seeds was
greater than that of un-primed seeds. Radicle length, seedling height and dry weight and leaf number of plants derived from
primed seeds were higher compared with un-primed seeds.
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After priming for 24 hours the soaked seeds were removed and rinsed with distilled water for three times and re-dried
to original moisture content under shade. These seeds were then sealed in polythene bags and stored in refrigerator for further
use.
Laboratory Germination Test and Seedling Vigour
For germination test three hundred seeds were randomly taken from each treatment of each variety of tomato,
brinjal and chilli. Three replicates of 100 seeds were germinated between double layered rolled germination paper along
with control (dry seeds) and moistened with sterile distilled water, in an amount equivalent to 2.5 times the mass of dry
substrate, made into rolls, and placed into a seed germinator at 25C. Germination was considered to have occurred when
the radicles were 3 mm long. The seedlings with short, thick and spiral form hypocotyls and stunted roots were considered
as abnormally germinated. Germination percentage was recorded on fourteenth day (final count). Observations were
recorded on germination percent, root length, shoot length, and seedling dry weight.
measured on 14th days. At the end of germination test (14 days), the seedlings were dried in an oven at 100 C for 24
hours. The dried seedlings were weighted to the nearest milligram and then the mean seedling dry weight was determined.
Vigour index I (seedling length x germination percentage) and vigour index II (seedling dry weight x germination
percentage) were then calculated.
Field Emergence
For field emergence (%) test three hundred seeds were randomly taken from each treatment and each variety of
tomato, brinjal and chilli. Three replicates of 100 seeds were sown in aluminium trays (100 cm x 90 cm size) having sandy loam
soil and coconut coir pith. Field emergence (%) count was done on 14th days. Observations were recorded on field emergence
percent, root length, shoot length, and seedling dry weight.
brinjal and chilli were measured on 14th days. At the end of field emergence count (14 days), the seedlings were dried in
an oven at 100 C for 24 hours. The dried seedlings were weighted to the nearest milligram and then the mean seedling dry
weight was determined. Vigour index I (seedling length x field emergence percentage) and vigour index II (seedling dry
weight x field emergence percentage) were then calculated.
Experimental Design and Statistical Analysis
Laboratory as well as field tests were carried out in a completely randomised design with three replications.
Analysis of variance (ANOVA) of laboratory and field data were calculated using SAS 9.3 version.
Estimation of Cumulative Germination Index, Cumulative Seedling Vigour Index and Seed Quality Index (SQI) of
Priming Treatments
The efficacy of different priming treatments was judged in terms of cumulative germination index, cumulative
seedling vigour index and seed quality index (SQI).These indices are calculated as follows. Using suitable class intervals,
the range of variability with regard to a character is classified into three groups such as low, medium and high. Each
character is scored as 0 for low value, 1 for medium value and 2 for high value. The cumulative germination index is
calculated by adding the scored values of lab germination percent and field emergence percent; cumulative seedling vigour
index is calculated by adding the scored values of lab SV-I & SV-II and field SV-I & SV-II.
Seed quality index is calculated by adding the scored values of the characters such as germination percentage,
Impact Factor (JCC): 4.7987
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vigour index I and II of lab emerged seedlings, field emergence (%) vigour index I and II of field emerged seedlings.
Higher SQI value produced by a priming treatment indicates its higher efficacy.
Here procedure is same as per paper entitled for study the first objective.
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Utkal Jyoti
92.33(73.98)
88.00(70.06)
84.00(66.42)
81.66(64.68)
83.66(66.20)
Utkal Keshari
91.00(72.60)
90.00(71.57)
85.66(67.76)
81.33(64.43)
83.00(65.67)
Utkal Madhuri
90.66(72.24)
90.00(71.81)
86.00(68.03)
83.00(66.48)
86.00(69.53)
Utkal Anushree
90.33(71.88)
92.00(73.57)
87.33(69.15)
85.00(67.21)
90.00(71.57)
Mean
91.08
90.00
85.75
82.75
85.67
NAAS Rating: 3.53
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Table 2: Contd.,
Ascorbic Acid (50 ppm)
Deionised H2O
Dry seed(Control)
CD(1%)
CV %
82.66(65.43)
78.00(62.03)
73.66(58.27)
3.47
2.21
82.00(64.90)
79.66(63.21)
71.66(57.84)
2.78
1.77
83.33(65.96)
82.66(65.41)
74.00(59.35)
4.78
2.98
83.00(65.65)
79.00(62.73)
74.33(59.56)
3.98
2.47
82.75
79.83
73.41
Figure 2: Effect of Priming Treatments in Increasing Germination Per Cent of Brinjal as Compared to Control
Root Length
Root length of brinjal varieties in response to different priming treatments is presented in Table 3. In Utkal Jyoti
root length was varied from 3.26 cm to 5.06 cm. Priming with Ascorbic Acid recorded the highest root length (5.06 cm)
followed by hydro priming (4.44 cm) and GA3 (3.90 cm). In Utkal Keshari Na2HPO4 recorded the highest root length
(5.20 cm) followed by hydro priming (5.00 cm) as compared to other treatments. In Utkal Madhuri Ascorbic Acid recorded
the maximum root length (5.82 cm) followed by PEG (5.78 cm). Ascorbic Acid and PEG both gave significantly higher
root length than control (5.14 cm) and GA3, KNO3, ZnSO4, and deionised water had produced lower root length than
control. Na2HPO4 produced the highest root length (5.95 cm) in Utkal Anushree followed by ZnSO4 (5.90 cm). GA3,
Na2HPO4, and ZnSO4 gave significantly higher root length than control. The mean effect of priming treatments showed
that Na2HPO4 was the best in increasing root length of brinjal.
Table 3: Effect of Priming Treatments on Root Length (cm) of Brinjal Varieties
Priming treatment Utkal Jyoti Utkal Keshari Utkal Madhuri Utkal Anushree
GA3 (1 ppm)
3.90
4.55
4.65
4.56
KNO3(5%)
3.42
4.66
4.38
4.09
Na2HPO4 (2%)
3.89
5.20
5.15
5.95
PEG (10%)
3.26
4.70
5.78
4.14
ZnSO4(1%)
3.74
3.83
4.00
5.90
Ascorbic Acid(50 ppm)
5.06
3.71
5.82
3.70
Deionised H2O
4.44
5.00
4.46
3.62
Dry seed(Control)
4.34
4.39
5.14
3.80
CD(1%)
0.22
0.50
0.38
0.46
CV %
2.28
4.66
3.23
4.28
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Mean
4.42
4.14
5.05
4.47
4.37
4.57
4.38
4.42
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Shoot Length
The effect of different priming agents on shoot length of different brinjal varieties is presented in Table 4. In
Utkal Jyoti shoot length was varied from 5.22 cm to 7.38 cm. Priming with Ascorbic Acid and Na2HPO4 recorded the
highest shoot length (7.38 cm) followed by KNO3 (7.01 cm). In Utkal Keshari the highest shoot length (8.18 cm) was
obtained with KNO3 followed by Na2HPO4 (7.17 cm) and the lowest was being recorded in Ascorbic Acid. In Utkal
Madhuri hydropriming recorded the highest shoot length (7.90 cm) followed by GA3 (7.52 cm) and PEG (7.46 cm). In
Utkal Anushree KNO3 recorded the highest shoot length (7.62 cm) followed by hydropriming (7.31 cm) and the lowest
was being recorded in PEG (4.96 cm). The mean effect of treatments revealed that KNO3 was the best treatment followed
by GA3 in increasing shoot length of brinjal.
Table 4: Effect of Priming Treatments on Shoot Length (cm) of Brinjal Varieties
Priming Treatment Utkal Jyoti Utkal Keshari Utkal Madhuri Utkal Anushree
GA3 (1 ppm)
6.87
6.73
7.52
6.93
KNO3(5%)
7.06
8.18
5.91
7.62
Na2HPO4 (2%)
7.38
7.17
6.33
6.43
PEG (10%)
5.22
6.34
7.46
4.96
ZnSO4(1%)
6.78
6.24
6.66
6.96
Ascorbic Acid(50 ppm)
7.38
6.15
7.41
6.28
Deionised H2O
5.56
6.25
7.90
7.31
Dry seed(Control)
6.48
6.20
7.45
6.26
CD(1%)
0.97
0.68
0.76
0.44
CV %
3.27
4.31
4.48
2.79
Mean
7.01
7.19
6.83
6.00
6.66
6.81
6.78
6.60
Brinjal
Figure 3
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four brinjal and two chilli varieties were treated with GA3 (1 ppm), KNO3 (5%), Na2HPO4 (2%), PEG (10%), ZnSO4
(1%), Ascorbic Acid(50 ppm) and Deionised H2O for 24 hours and then dried to original moisture content under shade.
Lab germination test was done following between paper method. For field emergence test primed seeds of different
vegetable varieties were sown in aluminium trays containing sandy loam soil mixed with coconut coir pith.
Observations were recorded on germination/field emergence, root length, shoot length, seedling length, seedling dry
weight, seedling vigour index-I & II in both lab and field tests. The experiments were done in a completely randomised
design with three replications. The classification of priming treatments was done through scatter plot of cumulative
germination index and cumulative seedling vigour index and the efficacy of priming treatments was expressed in term
of seed quality index (SQI). Results obtained in tomato, brinjal and chilli were presented below.
Brinjal
The mean effect of the treatments on germination of fresh brinjal seeds indicated that GA3, KNO3, Na2HPO4,
PEG, ZnSO4, Ascorbic Acid and deionised water produced higher germination by 24.07%, 22.6%, 16.8%,
12.7%, 16.7%, 12.7% and 8.75% respectively than control. This result revealed that GA3 and KNO3 had better
impact in improving germination of fresh brinjal seeds.
The mean effect of priming treatments showed that Na2HPO4 was the best treatment in increasing root length of
brinjal as compared to control and other treatments.
The mean effect of treatments on shoot length revealed that KNO3 was the best treatment followed by GA3 in
increasing shoot length of brinjal as compared to control and other treatments.
The mean effect of treatments indicated that GA3, KNO3 and hydrpriming had positive effect in increasing
seedling length as compared to control and other priming treatments.
Priming with KNO3 showed the maximum positive effect in increasing seedling dry weight of fresh brinjal seeds.
From the mean effect of the treatments it is observed that priming with GA3, KNO3, Na2HPO4, PEG, ZnSO4,
Ascorbic Acid and deionised water produced higher germination by 26.5%, 24.3% , 24.0% , 3.91%, 16.3% ,
14.8% and 10.5% respectively than control. The mean effect of priming treatments revealed that GA3 was more
potential in increasing SV-I value of fresh brinjal seeds.
From the mean effect it is evident that KNO3 was more efficient than GA3 and other treatments in increasing
SV-II value of fresh brinjal seeds..
Comparing the mean effect of all the treatments on FEP it was found that FEP increased by 25.8%, 23.1%, 17.5%,
14.1%, 17.3%, 13.08% and 9.03% when seeds were primed with GA3, KNO3, Na2HPO4, PEG, ZnSO4, Ascorbic
Acid and deionised water respectively as compared to unprimed seeds (Figure 3). This result indicated that GA3,
KNO3 and Na2HPO4 were efficient priming agents in increasing FEP as compared to others.
The mean effect of priming treatments on SV-I revealed that KNO3 has better impact on SV-I of FES in brinjal.
The mean effect of priming treatments on SV-II reflected the efficiency of ZnSO4 in increasing SV-II of FES
followed by PEG.
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REFERENCES
1.
Amooaghaie R, Nikzad K and Shareghi B. 2011. The effect of priming on emergence and biochemical changes of tomato seeds
under suboptimal temperatures. Seed Science Technology, 38:508-512.
2.
Araby MM and Hegazi AZ. 2004. Response of tomato seeds to hydro- and osmo- priming, and possible relations of some
antioxidant enzymes and endogenous polyamine fractions. Egyptian Journal Biology, 6: 81-93.
3.
Arjenaki FG, Dehaghi MA and Jabbari R. 2011. Effects of priming on seed germination of marigold (calendula officinalis).
Advantage Environmental Biology, 5: 276-280.
4.
Bradford KJ .1986.Manipulation of seed water relation via osmotic priming to improve germination under stress conditions
.Hort scientia 10,243-254.
5.
Bradford KJ, Steiner JJ and Trawatha SE. 1986. Seed priming influence on germination and emergence of pepper seed lots.
Crop Science, 30: 718-21.
6.
Chen K, Arora R and Arora U. 2010. Osmopriming of spinach (Spinacia oleracea L. cv. Bloomsdale) seeds and germination
performance under temperature and water stress. Seed Science Technology, 38: 36-48.
7.
Corbineau F, Ozbincol N, Vinol D and Come D. 2000.Improvement in tomato seed germination by osmopriming as related to
energy metabolism. In: Seed Biology: Advances and Application. M. Black, K.J. Bradford, Vazquez- Ramos J. (Eds.). CABI,
Oxon, UK, pp: 449-466.
8.
Dutta SK and Singh AR. 2015. Effect of priming on germination and seedling vigour of birds eye chilli seeds collected from
eastern Himalayan region of india. The Bioscan 10(1) 279-284.
9.
Eniseh A and Khourshid R. 2010. Effect of hydropriming on some physiological and biochemical parameters of safflower
(Carthamus tinctorius L.) cultivars under drought stress. Journal of Crop Ecophysiology, 1: 34-43.
10. Esmaielpour B, Ghassemi-Golezani K, Khoei FR, Gregoorian V and Toorchi M. 2006. The effect of NaCl priming on
cucumber seedling growth under salinity stress. Journal of Food, Agricultural Environment, 4: 347-349.
11. Farooq M, Aziz M, Barsa SMA, Cheema MA and Rehman H. 2008. Chilling tolerance in hybrid maize induced by seed
priming with salicylic acid. Journal Agronomy Crop Sciences, 194: 161-168.
12. Farooq M, Barsa SMA, Saleem BA, Nafees M and Chishti SA. 2005. Enhancement of tomato seed germination and seedling
vigour by osmo priming. Pakistan Journal Agriculture Science, 42(3-4): 36-41.
13. Farooq M,Basra SMA, Hussain M, Rehman H and Saleem BA.2007.Incorporation of Polyamines in the priming media
enhances the germination and early seedling growth in hybrid sunflower .International Journal of Agriculture and Biology
9:868-872.