Beruflich Dokumente
Kultur Dokumente
Contents
Abstract................................................................................................................. 2
Introduction........................................................................................................... 2
Environmental Stimuli............................................................................................ 3
Sound.................................................................................................................... 4
E-coli K12............................................................................................................... 5
The Mozart effect................................................................................................... 6
AIM......................................................................................................................... 7
Hypothesis............................................................................................................. 7
Variables:............................................................................................................... 7
Apparatus:............................................................................................................. 7
Method................................................................................................................... 8
Aseptic technique method..................................................................................... 8
Results................................................................................................................. 10
Results................................................................................................................. 11
Conclusion........................................................................................................... 11
Other research..................................................................................................... 12
Evaluation............................................................................................................ 13
Other Applications............................................................................................... 14
Photos from the experiment................................................................................ 14
Bibliography......................................................................................................... 15
Abstract
The biological effects of electromagnetic waves have been widely studied,
mainly due to their harmful effects, such as radiation-induced cancer and to their
application in diagnosis and therapy. The biological effects of sound, which is in
the form of a longitudinal wave which we are frequently exposed to have been
considerably disregarded by the scientific community. Although a number of
studies suggest that emotions evoked by music may be useful in medical care,
alleviating stress and nociception in patients undergoing treatments in cancer
and burned patients, little is known about the mechanisms by which these
effects occur. It is generally accepted that the mechanosensory hair cells in the
ear transduce the mechanical vibrations into neural impulses, which are
interpreted by the brain and evoke the emotional effects. Recently several
studies suggest that the response to music is even more complex, and evidence
has shown that cell types other than auditory hair cells could respond to audible
sound. Hence, the aim of the present study was to evaluate the response of the
harmless bacterium e-coli k12 to music. The results obtained suggest that music
can alter the growth in cultured cells. The results suggest that audible sounds
could modulate bacterial cell growth, by causing constant stress to the cell
resulting in inhibited growth.
Introduction
Type of bacteria
Photosynthetic
Bacteria
Enterobacter
Aerogenes
Description
Photosynthetic bacteria are a unique species of
microorganisms that use the sun as a source of energy.
Enterobacter aerogenes, part of the Enterobacteriaceae
Family, is a rod-shaped bacteria that causes bacterial
infections, and is usually acquired in a hospital or hospital-type
atmospheres. It usually causes opportunistic infections,
meaning that it will usually only cause a disease in a person or
host that has a compromised immune system. Studies are now
showing it causing increased alarm in community infections. It
rarely is known to cause a disease in someone with a healthy
immune system. These types of bacteria are extremely
sensitive to antibiotics but have the ability to become resistant
through their adaptive capabilities.
Francisella Tularensis
Francisella tularensis has been known to infect small mammals
such as rabbits, muskrats, mice, and also humans.
Interestingly however, is that no case of tularemia has
appeared to be caused through human to human contact.
Infection has appeared to always be caused by contact with
infected animals or forms of transmission such as mosquitoes
or ticks that have bitten infected animals.
Helicobacter Pylori
These types of bacteria are found in the stomach. H. pylori, a
major cause of stomach and other gastro esophageal issues
such as gastric ulcers, chronic gastritis, duodenal ulcers, and
even stomach cancer flourishes in the upper gastrointestinal
tracts of at least half the worlds population.
Bacteria are microorganisms that cannot be seen by the naked eye, they exist in
all environments on Earth. The human body, hosts more bacterial cells within
than it has total cells comprising the body. There are a diverse species of
bacteria, these are summarised in the table below. (ScienceDaily, 2016)
disappear when the stress is over, others are maintained and can even be
passed on to surviving bacteria.
Bacterial adaption and stress response network (Mackert, 2016)
Environmental Stimuli
Research has been carried out focusing on the investigation of bacterial stress
responses and the effect of external environmental changes. (Goldstein and Soyer,
2008) A better knowledge of the bacterial stress response include the
identification of stress-inducing effectors and key molecular switches is the basis
of understanding and controlling bacterial growth in any environmental setting.
There are vasts amount of environmental stimuli that can cause a stress
response to bacteria. The bacteria are able to sense and respond to a variety of
external stimuli with responses that vary from stimuli to stimuli and from species
to species, the bacterium will move to the most favourable location where there
is plenty of food, enough light and are not stressed. (Foodsafetysite.com, 2016)The
best-understood in current research is chemotaxis in Escherichia coli, where the
dynamics and structure of the underlying pathway is well characterised. There is
increasing experimental evidence that bacteria integrate responses from
different stimuli to generate a coherent taxis response. There is currently a lack
of understanding of the different pathway structures and dynamics and how this
integration is achieved. (Goldstein and Soyer, 2008)
Examples of external stimuli include changes to temperature, light, nutrients and
sound.
Sound
Sound travels through waves which are created by the vibration of an object,
which causes the surrounding air to vibrate. The waves are unique to each sound
causing different patterns of vibration. (PBS LearningMedia, 2016) When a speaker
vibrates from emitting music, the surrounding molecules vibrate in the same
unique pattern which can affect the growth of bacteria in a specific way. Bacteria
lack the ability to hear music, but are sensitive to the environmental vibrations
that they are growing in.
There are four phases of bacterial growth.
Lag Phase: growth is very slow
Log Phase: Begins to multiply exponentially
Stationary Phase: growth stops and stabilises
Death Phase: toxic waste builds up, the cell dies. (Vlab.amrita.edu, 2016)
E-coli K12
(Bats.ch, 2016)
(Scienceblogs.com, 2016) The graph shows an increase in spatial IQ test score when listening to
mozart.
Control- The petri dish without music but contains e-coli k12
Independent- The type of music played to a petri dish containing e-coli k12
Dependant- The growth of bacteria in each sample
Constant- The temperature and location.
Apparatus:
To make
Nutrient
agar
Distilled Agar powder
water
Hotplate Magnetic
Stirrer
Flask x 1 Beaker x 1
To conductBunsen
Saucepan
theBurner
experiment
Heated Petri dishes x
container 6
To plate
streak petri
dishes
e-coli k12 2 x
inoculating
loops
6 x Sterile Verkon
petri dishes disinfectant
at 9 cm
Distilled Cloth
water
1 x Tray Marking pen
Storage at Bunsen
room burner
temperature
Paper
template
Method
Creating nutrient agar:
1.
2.
3.
4.
5.
Image from the experiment, showing headphones attached to the independent petri
dish
Results
The bacteria were left in a silent room with plenty of light, the temperature of the
room stayed at approximately 23oC for the duration of 48 hours. The results were
collected after 12 24 and then 48 hours in order for the e-coli to establish grown
colonies. The table below shows the amount of colonies counted in the time
given.
Colonies
established
Independent
variable
Control variable
After 12 hours
After 24 Hours
After 48 Hours
59
100
283
After 12 hours of incubation time, no bacteria were found to be alive in the petri
dish, after 24 hours of incubation time very few colonies were found in the
independent petri dish, but more were found to be colonised in the control petri
dish. The results continued to show an increase in the amount of colonies in the
control petri dish and established in a 24 hour period approximately 283 live
colonies of e-coli k12, compared to approximately 58 in the petri dish that was
exposed to music.
24 Hours
12 Hours
0
50
100
150
Control Colonies
200
250
300
Independent Colonies
24 Hours
Control Colonies
48 Hours
Independent Colonies
Results
The inverted
image from
the
experiment
shows the
grown
cultures in
the
independent
and control
petri
dishes
Conclusion
In accordance with my hypothesis
the
results show that music does affect
microbial growth, but the data shows that it does not affect the growth in a
positive way. The control and independent petri dishes were left in exactly the
same room, at the same temperature with the same amount of light. The e-coli
k12 in the control petri dish grew more colonies than the e-coli k12 in the
independent petri dish. The incubation time stayed the same. This may be
because of overstimulation or stress , but at this time remains unknown because
of limitations to the experiment. Current research suggests that the Mozart effect
has a positive impact on the growth of bacteria (Lrs.ed.uiuc.edu, 2016), my results
do not correlate with the current research. Research also suggests that the
harmony in Mozart corresponds to the harmony that binds and breaks down the
bonds between molecules. (Academia.edu, 2016) The growth of bacteria can be
inhibited when the vibration overstimulates the cell causing cell-lysis. (SigmaAldrich, 2016) The Mozart track was played to the independent dish, for a full 48
hours with no stoppage time. This may have caused the molecules within the cell
to vibrate rapidly causing the inhibited growth. Both the control and independent
petri dish had a slow incubation time, nothing grew after 12 hours. The line
graph shows that, as the lag phase of the bacteria started to occur the difference
between the independent petri dish only established 2 colonies, whereas the
control had 50 colonies. This was after 18 hours. The log phase then occurred
after 18 hours where the cells multiplied exponentially and the growth sped up,
after 27 hours the control had established 160 colonies, and the independent
had 20 colonies. This could suggest that the vibrations in the music caused the
bacteria to stay in lag phase due to overstimulation. Overall there was a
130.99% difference in grown colonies between the control and independent. This
can be worked out by:
= ( | 59 - 283 | / ((59 + 283)/2) ) * 100
= ( | -224 | / (342/2) ) * 100
(Jang, 2013) The bar graph shows the difference in the number of bacterial colonies of
each variable.
The graph conduced in the study on the effects of microbial growth shows that
the rock music the bacteria was exposed to had a faster growth rate after the
second measurement than in the controlled petri dish. Classical music had a
negative effect on growth rate. The results show that during the lag phase (1 st
measurement) music could have been causing a prolonged period of lag phase.
Which correlates to my results, both in the classical and rock music 1 st
measurement growth was slowed down. (Jang, 2013)
A study by Shaobin G. showed that audible sound had a negative effect on e-coli
when the e-coli was exposed to a stress factor. Shaobin G. Investigated the
response of Escherichia coli cells to the stimulation by audible sound under the
normal condition and environmental stresses. The results showed that the
audible sound treatment significantly increases the colony forming of E. coli
under the normal growth condition. However, under osmotic stress induced by
the sugar, audible sound stimulation may enhance the inhibitory effect of
osmotic stress on E. coli growth. More interestingly, audible sound treatment
seems to alleviate the inhibitory effect of salt stress on E. coli growth when the
concentration of sodium chloride was increased to 30 g/l, although the action of
sound waves of audible frequency is likely to evoke an inhibition of the growth of
E. coli in the medium containing 20 g/l of sodium chloride. Some potential
mechanisms may be involved in the responses of bacterial cells to audible sound
stimulation. (Shaobin G, 2016)
An experimental investigation conducted Lee Ying on the effects of audible
sound on the growth of e-coli found that at selected frequencies of 1kHz, 5kHz,
and 15 kHz had increases the number of viable cells in a petri dish. Thus showing
that the bacteria reacted positively to the sound treatment which resulted in a
faster growth of the e-coli. (Lee Ying, Dayou and Phin, 2009)
The graph shows classical music grew faster than the control and heavy metal music,
suggesting the bacterial growth was not inhibited. . (Chow, 2014)
Evaluation
There were a lot of limitations with this experiment, the e-coli k12 had a very
unusual long incubation time, and was presumed dead after 12 hours, only after
18 hours did colonies become visible. The room temperature was recorded at
23OC during the daytimes after every 12 hours, but this could have changed
during the night as there was no one in the laboratory to observe the room
temperature. The change in temperature could have caused the unusually long
incubation time but could not have affected results due to the independent and
control variables staying in the same room. Light availability may have also
affected results. The control may have been left in a slightly lighter area than the
independent petri dish, causing the bacteria to multiply faster. The plate
streaking technique may have played an important role in causing error to the
experiment. This is because there could be more e-coli k12 on the inoculating
loop, meaning there was more cells on the plate than the other to start with,
making division occur more often.
The growth of bacteria was not measured accurately and the colonies counted
were an approximation, the experiment could have been made more accurate by
having several trials. The type of bacteria used in experiments may also have
different susceptibilities, pH readings were also not taken. Taking a pH reading
would have improved accuracy in the experiment because a low or high pH could
have been ruled out as an influencing factor for the inhibited microbial growth In
the independent dish.
In order to improve the experiment, there should have been many more control
and independent petri dishes, which would give better results, if music has
inhibited the growth of bacteria, all independent dishes would have much less
colonies than the control dish. Another way to improve the experiment and
prevent over stimulation is to vary the amount of music given to the independent
variable, different types of music also could have been used to determine
whether music affects microbial growth.
Other Applications
Bacteria are perceptive to changes in surrounding vibrations, which may suggest
that music has an effect on bacterial cells inside animals. A slower or faster
growth rate may affect how a cell becomes resistant to antibiotics. According to
the results of the experiment, if an animal is exposed to a lot of classical music
bacterial growth may be inhibited. Music could also speed up or slow down other
microbial assisted duties, such as those in a sewage system, decreasing energy
costs and increasing productivity. If music has a positive effect on bacteria,
insulin could be made quicker in a laboratory.
Bibliography