Digital Re-print - May | June 2010

Feature title: Simultaneous determination of mycotoxins

- using immunoaffinity columns in conjunction with HPLC or LC-MS/MS

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Simultaneous
determination of
mycotoxins using
immunoaffinity columns
in conjunction with
HPLC or LC-MS/MS
If such testing (as outlined below) is used in conjunction with preventative measures at all stages
in the production process, contamination risk to the consumer will be significantly reduced
A
flatoxin and Ochratoxin A were
Over the years, legislation for mycotoxins
some of the first mycotoxins
in food has increased to incorporate
of interest and are produced
additional matrices and toxins, such as
by Aspergillus and Penicillium fungi
the more commonly occurring Fusarium
when foods such as nuts, cereals,
mycotoxins.
dried fruit, herbs, spices and coffee
This increase has placed pressure on
are stored under adverse conditions
laboratories to perform multiple mycotoxin
of temperature and humidity.analysis on an ever-increasing range of
commodities. The result of
this is an increased demand for
faster and less labour intensive
“Over the years, legislation for tests to allow more analyses
to be performed in a shorter
time period.
mycotoxins in food has increased to Currently, there are a wide
variety of tests available for
incorporate additional matrices and the detection of mycotoxins.
These include ‘traditional’
toxins, such as the more commonly m e thods such as thin
layer chromatography and
occurring Fusarium mycotoxins” more rapid tests such as
immuno af f init y columns ,
enzyme immunoassays or
36 | may - June 2010
Feature
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Mycotoxins
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Better quality control.
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Eliminates unnecessary fumigation.
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Improved Health & Safety standards, when compared to
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Improved pest detection allows stores, mills and export
® silos to ensure they do not accept or supply customers
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Tel : +44 (0) 1692 403403 Fax : +44 (0) 1692 404955
Web : http://www.tekpro.com Email : sales@tekpro.com
Grain &feed millinG technoloGy may - June 2010 | 19
R-Biopharm Rhône Ltd.
GFMT10.03.indd 19 28/05/2010 10:16
Multi-Mycotoxin
Immunoaffinity Columns
lateral flow devices commonly known as
dipstick tests. • Time, labour and cost saving
R-Biopharm’s immunoaffinity columns • A single extraction protocol simplifies analysis
offer a simple and rapid solution, allowing • Simultaneous detection of mycotoxins in a single run
legislative levels to be easily reached • Extremely sensitive, exceeding regulatory limits
quantitatively by HPLC or LC-MS/MS. • Highly reproducible results
• Manufactured under ISO Registered Quality Systems
Multi analysis in conjunction with
either HPLC or LC-MS/MS
R-Biopharm's Immunoaffinity Columns
have been developed to easily clean up and concentrate
Immunoaffinity columns are the method
analytes from many complex matrices for analysis by
of choice for the sample preparation for HPLC & LC-MS/MS
regulatory mycotoxin analysis however Columns available for single or multi-mycotoxin determination
there is a growing requirement for columns,
Mycotoxins
that can offer multi analysis in conjunction
with either HPLC or LC-MS/MS using a
Total Aflatoxins Ochratoxin A Fumonisins
Aflatoxin M1 Zearalenone T-2 & HT-2
single extraction method and analytical run.
Deoxynivalenol
R-Biopharm has developed a number
of new multimycotoxin immunoaffinity
columns. Aflaochra Prep® and Easi- R-Biopharm Rhône Ltd.
Extract® T-2 & HT-2 are for use in Block 10 Todd Campus Phone: +44 (0) 141 945 2924
West of Scotland Science Park Fax: +44 (0) 141 945 2925
conjunction with HPLC. The most recent
Acre Road, Glasgow info@r-biopharmrhone.com
addition to its product line, DZT MS-Prep®,
Scotland G20 0XA www.r-biopharm.com
is designed for simultaneous detection of
Grain &feed millinG technoloGy Grain &feed millinG technoloGy PREVIOUS PAGE NEXT PAGE may - June 2010 | 37

“The multi-mycotoxin columns
are manufactured using
monoclonal antibodies which
are bound to a solid support”
deoxynivalenol, zearalenone, T-2 and HT-2
in conjunction with LC-MS/MS.
The multi-mycotoxin columns are immunoaffinity columns is that only one
manufactured using monoclonal antibodies sample preparation method is required for
which are bound to a solid support.
This enables isolation and concentration run therefore allowing greater sample
of the target mycotoxin with high specificity throughput and a reduction in the use of
and improved sensitivity.
Extraction by blending the food
or feed sample with a solvent
In all cases the toxins are extracted by
blending the food or feed sample with a preventative measures at all stages in the
solvent, the extract is then filtered, diluted production process, contamination risk to
and a small volume passed through the the consumer will be significantly reduced.
immunoaffinity column by the analyst. If
Figure 1: Overview of Immmunoaffinity column procedure
38 | may - June 2010
News
the target mycotoxin
is present in the test
sample, it is selectively
bound to the antibody
in the column ; the
toxin is then released
from the column using
a solvent. The eluate
is injected onto the
H P LC o r LC - M S /
quantification
MS system ready for
(see Figure 1).
The advantage of these new
quantifying several mycotoxins in a single
solvents and consumables.
The development of faster, accurate
tests enables easier and more thorough
monitoring of foods by the supplier. If
such testing is used in conjunction with
R - B i o p h a r m h a s ove r 2 0 ye a r s
PUB85x254-T5X 28/01/10 14:22 Page 1
Mycotoxins Feature
May - June 2010 ®
pelleting solutions
can access replacement parts as wide range of industries,
quickly as possible. including animal feed,
In addition, the Sizer team has food and drink processes,
extensive technical knowledge biomass and Refuse
and expertise to ensure the Derived Fuel (RDF).
Sizer....The future of
company can provide valuable In addition, Sizer has pelleting since 1899
advice on all aspects of pelleting. the benefit of spare parts
Later this year, Sizer Ltd has distribution centres in the
exciting plans to launch its own UK and Holland, providing
range of small pellet machines, customers with the
ideal for laboratory testing of highest levels of service,
pellets or for domestic use. while also partnering with
“We offer a very flexible international distributors in
service, which means we can be countries such as Canada,
responsive to customer needs, Japan & Australia.
whether in the UK or overseas.
Customers will also be able More inforMation
to benefit from our ongoing Sizer Limited Whether you are looking for a pelleting press,
maintenance contracts to ensure Newburgh, Bessemer
a full production line or just spare parts,
their pellet machines are running
to optimum efficiency, which helps
Way, Rotherham, South
Yorkshire, S60 1FB Sizer Pelleting Solutions makes a positive There are other
difference financially and environmentally.
ways to bind
United Kingdom
to minimize the risk of downtime,”
says Roger Pearson. Tel: +44 1709 724260
Telephone: +44(0)1709 724279 or email us:
Orbit pellet presses can be
Fax: +44 1709 839312
Email: info@sizer-pelleting.co.uk Suzanne Birley: suzanne.birley@sizer-pelleting.co.uk mycotoxins…
found across the world in a
www.sizer-pelleting.co.uk
T5X
Website: www.sizer-pelleting.co.uk
The parent company of Richard Sizer Ltd. Established 1899
www.aviana.co.ke info@aviana.co.ke
Silo storage expanded at Brice Baker 8.6x12.4 Ad AW 21/9/06 3:38 pm Page 1
Camgrain APC
experience in the development of various
much more than
T
test formats, methods and services for the
he new Camgrain APC decade of population
detection of mycotoxins. The company
site in the UK, which is in increase and changes in diet, The Complete a Toxinbinder
plans to continue to develop tests for
additional to the 150,000 have highlighted the likely
other mycotoxins ahead of legislation
tonne plant at Linton just a few world-wide shortage of
and to provide the complete mycotoxin
miles away is now supplying foodstuffs and cereal crops.
BRICE-BAKER Solution T5X has four major actions :
solution for both large and small food
wheat destined for bread making Brice Baker has reacted
companies. T5X binding components have been
exclusively for Sainsburys. to this development Brice-Baker has completed projects in
selected in a sophisticated “in vivo”
More inforMation: positively plans to invest 22 countries. We supply high quality, model. Mycotoxins do not pass through
Having
R-Biopharm AG previously supplied 12 in new equipment for the
reliable products, supported by good service, the intestinal wall.
5000-tonne storage
An der neuen Bergstraße 17 silos on this manufacture of silos using
new site,
64297 Brice Baker
Darmstadt, had last year the latest corrugating plant
Germany provided by experienced professional staff. T5X stimulates the production of specific
supplied
Tel: +49 61four 12-metre diameter and the purchase of steel in
51 81020 natural detoxication enzymes to catalyse
Fax:
wet+49 61 51hopper
grain 810220 silos, with coil form. the elimination of the mycotoxins.
Email: info@r-biopharm.de
aeration, to give a combined total “The present factory
Website: www.r-biopharm.com T5X powerful antioxidants inhibit free
capacity of around 4200 tonnes of is no longer suitable and radicals and prevent cells' membrane
pre-drying storage. this investment has also from degradation.
Brice Baker also supplied the enabled us to seek for new
40-metre conveyor bridge over premises to allow the plant T5X stimulates the non specific immune
the driers to feed the new silos, to work efficiently,” says the system to strengthen animals
designed to carry four 300-tonnes- company. Brice-Baker bulk storage silos +
per-hour conveyors. Alongside this materials handling + drying and cleaning
An additional 30,000 tonnes investment, new designs equipment + planning and installation by
of flat bunker storage was added are being investigated so experienced professional staff + good service
with under floor discharge that Brice Baker silos will = The Complete Solution.
systems. Brice Baker also supplied be easier to manufacture
conveyors, screw conveyors and and erect using standard Additives & difference
T: +44 (0)1480 216618 | F : +44 (0)1480 406226
elevators totalling around 1.6 km components. Deliveries
www.bricebaker.co.uk | E : info@bricebaker.co.uk
of either 300 or 600-tonnes-per- across the world will also be
made simpler by introducing a brand of the B.P. 394 - 56 009 VANNES Cedex - FRANCE
hour conveying equipment. Romer Labs ® UK Ltd - Ireland & UK
Tel: +44 845 519 5010, Fax: +44 1745 827150 group Neovia@evls.net
Predictions over the next such changes. e-Mail: enquiry@romerlabs.com
&feed millinG technoloGy
&feed&
Grain feed millinG technoloGy Grain PREVIOUS PAGE NEXT PAGE may - June 2010 | 39
Grain millinG technoloGy may - June 2010 | 5
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