FOOD HANDLER &IQAMA STOOL

CULTURE
Inoculate stool sample on Selenite F broth and Alkaline
Peptone water then incubate it at 37 C for 10-12 hours

Subculture from Selenite F to XLD agar

Subculture from Alkaline Peptone water to TCBS
Incubate agar plate at 37 C for 24 hours

For Salmonella and Shigella- pick one colony of each

morphologic type of red to red orange colony and any colony
with black centre from XLD .
Subculture each colony picked to TSI and Urea agar. If urease
test positive (pink) discard the sample, if negative proceed
to TSI agar-if TSI agar shows acid butt(yellow) and alkaline
slant (red) proceed to next step otherwise discard the
sample
Confirm Salmonella and Shigella organism by API 20E
Test for agglutination for salmonella and shigella (Serology)
For Cholera- pick Yellow colored colonies from TCBS are
taken and confirmed by API 20E
Oxidase and Cholera red reaction is also done to identify
Vibrio

 Test for agglutination for Vibrio (Serology)