Nama enzim: Lactate dehydrogenase

Reaksi katalisis enzim Lactate dehydrogenase

Sumber/asal isolasi enzim Lactate dehydrogenase:

1. Hati
2. Ginjal
3. Otot
4. Otak
5. Sel darah merah
6. Bakteri asam laktat
Inhibitor dari enzim Lactate dehydrogenase:
INHIBITORS

ORGANISM

COMMENTARY

3-aminopyridine adenine
dinucleotide

duck

competitive versus NAD+ and noncompetitive

versus L-lactate <42>

1,6 Hexanediol
3-fluoropyruvate

Ralstonia eutropha

3-phosphoglycerate

Lactuca sativa

6-phosphogluconate

Lactobacillus casei

ADP

Bacillus caldolyticus

ADP

Ipomoea batatas

ADP

Lactobacillus casei

ADP

Lactobacillus plantarum

ADP

Lactuca sativa

ADP

Selenomonas
ruminantium

ADP

Solanum tuberosum

ADP

Staphylococcus
epidermidis

ADP

Streptococcus mutans

Ag+

Bacillus subtilis

Ag+

Ralstonia eutropha

Ala

Lactuca sativa

alpha-NAD+

duck

noncompetitive inhibitor versus beta-NAD+

<42>

AMP

Ipomoea batatas

isoenzyme I and II, inhibition is reduced by

MgCl2 <16>

AMP

Lactuca sativa

AMP

Staphylococcus
epidermidis

isoenzyme I and II, inhibition is reduced by

MgCl2 <16>

competitive with NADH <6>

ATP

Acholeplasma laidlawii

ATP

Actinomyces viscosus

ATP

Ipomoea batatas

ATP

Lactobacillus casei

ATP

Lactobacillus plantarum

isoenzyme I and II, inhibition is reduced by

MgCl2 <16>
competitive with respect to NADH at pH 7.0
and at pH 6.2 <18>

ATP

Lactuca sativa

ATP

Pediococcus
pentosaceus

ATP

Rothia dentocariosa

ATP

Selenomonas
ruminantium

ATP

Solanum tuberosum

ATP

Staphylococcus
epidermidis

ATP

Streptococcus cremonis

Cd2+

Lactobacillus casei

at high concentration <1>

Cd2+

Lactobacillus helveticus

0.1 mM and 1.0 mM, weak inhibition <41>

Cd2+

Ralstonia eutropha

partial <11>

citrate

Lactuca sativa

citrate/phosphate buffer

Lactobacillus casei

at pH 5.4 <5>

Co2+

Lactobacillus helveticus

0.1 mM and 1.0 mM, weak inhibition <41>

Cu2+

Lactobacillus casei

CuSO4 <1,5>

Cu2+

Lactobacillus helveticus

0.1 mM and 1.0 mM, weak inhibition <41>

Cu2+

Nocardia asteroides

CuCl2 <36>

Cu2+

Pediococcus
pentosaceus

CuSO4 <1>

Cu2+

Ralstonia eutropha

D-lactate

duck

at neutral or alkaline pH ATP behaves as a

weak competitive inhibitor, potent inhibitor at
acid pH values <12>

dead-end inhibitor, competitive inhibitor

versus L-lactate <42>

dihydroxyacetone phosphate Lactobacillus casei

DTNB

Homarus americanus

fructose 1,6-diphosphate

Lactuca sativa

fructose 1,6-diphosphate

Thermus caldophilus

glucose 6-phosphate

Lactuca sativa

Gly

Lactuca sativa

GTP

Lactobacillus casei

GTP

Solanum tuberosum

GTP

Staphylococcus
epidermidis

HEPES buffer

Lactobacillus helveticus

Hg2+

Acinetobacter
calcoaceticus

HgCl2 <21>

Hg2+

Lactobacillus casei

HgCl2 <1,5>

Hg2+

Molinema dessetae

Hg2+

Pediococcus

activation at low concentrations, inhibition at

high concentrations <14>

HgCl2 <1>

pentosaceus
Hg2+

Ralstonia eutropha

iodoacetamide

Lactobacillus acidophilus slight <1>

iodoacetamide

Lactobacillus casei

iodoacetate

Lactobacillus acidophilus Lactobacillus curvatus: no effect <24>

iodoacetate

Lactobacillus casei

Lactobacillus curvatus: no effect <24>

iodoacetate

Molinema dessetae

Lactobacillus curvatus: no effect <24>

isocitrate

Lactuca sativa

L-lactate

duck

lactate analogs

Bacillus subtilis

MES buffer

Lactobacillus helveticus

Mn2+

Lactobacillus casei

at low pH-values <19>

more

Plasmodium falciparum

lack of substrate inhibition <29>

NAD+

Ipomoea batatas

competitive with respect to NADH, reduction

of pyruvate <16>

NAD+

Lactobacillus casei

NAD+

Pediococcus
pentosaceus

NAD+

Ralstonia eutropha

NAD+

Selenomonas
ruminantium

NAD+

Solanum tuberosum

product inhibition <12>

NADH

Solanum tuberosum

competitive with respect to NAD+ <12>

NADPH

Thermoanaerobacter
ethanolicus

Ni2+

Ralstonia eutropha

partial <11>

nicotinic acid adenine

dinucleotide

duck

competitive versus NAD+ and noncompetitive

versus L-lactate <42>

oxalate

Acinetobacter
calcoaceticus

oxalate

Lactuca sativa

oxalate

Molinema dessetae

oxalate

Pediococcus
pentosaceus

oxalate

Ralstonia eutropha

oxalate

Solanum tuberosum

oxaloacetate

Bacillus subtilis

oxaloacetate

Ralstonia eutropha

oxamate

duck

oxamate

Lactobacillus acidophilus

oxamate

Lactobacillus plantarum

oxamate

Molinema dessetae

oxamate

Pediococcus
pentosaceus

oxamate

Ralstonia eutropha

oxamate

Staphylococcus
epidermidis

slight <1>

substrate inhibition is uncompetitive <42>

noncompetitive with pyruvate, competitive

with lactate <30>

dead-end inhibitor, competitive inhibitor

versus pyruvate <42>

competitive with pyruvate, noncompetitive

with lactate <30>

oxamate

Streptococcus cremonis

oxamate

Streptococcus mutans

p-chloromercuribenzoate

Bacillus subtilis

p-chloromercuribenzoate

Molinema dessetae

p-chloromercuribenzoate

Ralstonia eutropha

p-chloromercuribenzoate

Streptococcus cremonis

p-hydroxymercuribenzoate

Homarus americanus

p-hydroxymercuribenzoate

Lactobacillus acidophilus slight <24>

p-hydroxymercuribenzoate

Lactobacillus casei

p-hydroxymercuribenzoate

Lactobacillus helveticus

p-hydroxymercuribenzoate

Pediococcus
pentosaceus

phenylglyoxal

Fundulus heteroclitus

phosphate

Lactococcus lactis

phosphate

Thermus caldophilus

phosphoenolpyruvate

Ipomoea batatas

phosphoenolpyruvate

Ralstonia eutropha

phosphoenolpyruvate

Solanum tuberosum

pyruvate

duck

substrate inhibition is uncompetitive <42>

pyruvate

Homo sapiens

above 0.3 mM <40>

pyruvate

Lactococcus lactis

above 20 mM <25>

pyruvate

Plasmodium falciparum

substrate inhibition in wild type enzyme,

lower substrate inhibition in mutant S163L
<29>

pyruvate

Saduria entomon

at high concentrations <32>

pyruvate

Thermus caldophilus

strong substrate inhibition at high

concentrations, fructose 1,6-diphosphate
activated enzyme <14>

Ser

Lactuca sativa

sodium phosphate

Lactobacillus casei

tartronate

duck

dead-end inhibitor, competitive inhibitor

versus L-lactate <42>

thionicotinamide adenine
dinucleotide

duck

competitive versus NAD+ and noncompetitive

versus L-lactate <42>

Tris-HCl

Lactobacillus helveticus

Tris/maleate buffer

Lactobacillus casei

Zn2+

Ralstonia eutropha

slight activation of non-activated enzyme,

inhibition of fructose 1,6-diphosphate
activated enzyme <14>

at pH 5.4 <5>
-

Nama : Fendy
NIM : 10504011