L-8900 Instruction Manual For Software

INSTRUCTION MANUAL
FOR
L-8900 HIGH SPEED AMINO ACID ANALYZER
(EZChrom Elite for Hitachi AAA Operation)
Please read through this manual

carefully and store it in a safe place.
Before using the instrument, read the
safety instructions and precautions
carefully.
Keep this manual in a safe place nearby so
it can be referred to whenever needed.
This manual pertains to the main unit of Model

L-8900 high speed amino acid analyzer.
For details of PC functions, refer to the
separate EZChrom Elite manuals.
Hitachi High-Technologies Corporation
Copyright Hitachi High-Technologies Corporation 2005. All rights reserved. Printed in Japan.
Part No. 852-9011-1 TG-TH (NH-LH)
Be sure to read through and understand the following points

with regard to this manual.
1. Information contained in this document is subject to
change without notice for improvement.
2. This manual is copyrighted by Hitachi High-Technologies
Corporation with all rights reserved.
No part of this manual may be reproduced, transmitted
or disclosed to a third party in any form or by any means
without the express written permission of Hitachi
High-Technologies Corporation.
3. Hitachi High-Technologies Corporation assumes no
liability for any direct, indirect, or consequential damages
arising from use not described in this manual.
Utmost care must be exercised when using the
instrument.
4. This document does not provide any warranty or
permission for industrial properties or any rights to grant
license lawfully and without infringement.
PREFACE
This instruction manual (EZChrom Elite operation) describes the
operating procedures for directly controlling the EZChrom Elite for
HITACHI Amino Acid Analyzer software without using the
AMINOOASYS (L-8900 simplified navigation software).
Before attempting operation of the Model L-8900 high-speed
amino acid analyzer, read carefully this instruction manual and the
main unit manual prepared separately to attain a clear
understanding of the instrument for its proper handling and use.
Note that physiologically or biologically active samples are not
applicable to this instrument because of possible infection with
bacteria or viruses.
This instrument is intended for use by persons having a basic
knowledge of chemical analysis procedures. Keep in mind
that improper use of analytical instruments, chemicals or
samples would result not only in wrong analytical data but
also in consequences adverse to safety.
For details of the EZChrom Elite, refer to the EZChrom Elite user
manual prepared separately.
NOTE: For navigation of the EZChrom Elite, use the mouse
and keyboard. Note that the EZChrom Elite does
not support touch-sensitive panel functions.
ABOUT THIS MANUAL

This instruction manual has been prepared for the user of the
Model L-8900 high speed amino acid analyzer (EZChrom Elite for
Hitachi AAA Operation). The operating procedures and
cautionary instructions regarding the instrument are contained in
this manual.
First of all, please read IMPORTANT and SAFETY
SUMMARY at the beginning of the organizer instruction
manual.
For details of each product designed for the Model L-8900, refer to
the instruction manual accompanying it.
IMPORTANT
Warranty on Product
This product is warranted to be free from defects in material or
workmanship under normal use within the product specifications
indicated in this manual and under conditions given below.
This warranty is void if the instrument is not used according to the
instruction manual.
The manufacturer makes no warranties, either express or implied,
except as provided herein, including without limitation thereof,
warranties as to marketability, merchantability, for a particular
purpose or use, or against infringement of any patent.
No oral or written information or advice given by the manufacturer,
its dealers, distributors, agents or employees shall create a
warranty or in any way increase the scope of this warranty.
(1)
Scope of Warranty
Any parts that prove to be defective in design or
workmanship during the warranty period will be repaired,
adjusted or replaced without charge. A substitute part may
be used for repair, or replacement with an equivalent product
may be made instead of repair. Such system components
as a personal computer and printer to be updated frequently
for improvement may not be available in original versions at
the time of replacement.
Note that this warranty does not apply to the instrument after
it is discarded, or if modified by the user or resold without
permission from the manufacturer, consumable parts, and
any failure of lifetime-expired parts.
The manufacturer assumes no liability for any damage to
data or application software due to any possible fault or
failure of this instrument.
(2)
Warranty Period
One year from the date of initial installation
(In case a separate warranty document has been issued, the
warranty period indicated in it takes precedence over the
above period.)
IMPORTANT - 1
(3)
Limitations and Exclusions on Warranty

Note that the following cases are excluded from the scope of
this warranty.
(a)
Failure due to operation at a place not meeting the

installation requirements specified by the manufacturer.
(b)
Failure due to power supply voltage/frequency other

than specified by the manufacturer or due to
abnormality in power supply.
(c)
Corrosion or deterioration of the piping due to

impurities contained in gas, compressed air or cooling
water supplied by the user.
(d)
Corrosion of the electric circuits or deterioration of the

optical elements due to highly corrosive atmospheric
gas.
(e)
Failure due to use of software, hardware or spare parts

not supplied by the manufacturer.
(f)
Failure due to use not described in the manual or

improper repair not approved by the manufacturer.
(g)
Failure due to maintenance or repair by other than

service personnel qualified by the manufacturer.
(h)
Failure due to relocation or transport conducted not

under the supervision of the manufacturer after the
initial installation of the instrument.
(i)
Failure due to disassembly, modification or relocation

not approved by the manufacturer.
(j)
Failure due to acts of God, including fire, earthquake,

storm, flood, lightning, social disturbance, riot, crime,
insurrection, terrorism, war (declared or undeclared),
radioactive pollution, contamination with harmful
substances, etc.
IMPORTANT - 2
(4)
(k)
Failure of the hardware, or damage to the system

software, application software or data due to computer
virus infection.
(l)
After disposal of this instrument, after its resale without

prior approval from the manufacturer, consumable
parts, and failure of any part that have reached the end
of its service life.
Disclaimer of Warranty
THE MANUFACTURER MAKES NO WARRANTIES,
EITHER EXPRESS OR IMPLIED, EXCEPT AS PROVIDED
HEREIN, INCLUDING WITHOUT LIMITATION THEREOF,
WARRANTIES AS TO MARKETABILITY,
MERCHANTABILITY, FOR A PARTICULAR PURPOSE OR
USE, OR AGAINST INFRINGEMENT OF ANY PATENT.
IN NO EVENT SHALL THE MANUFACTURER BE LIABLE
FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL
DAMAGES OF ANY NATURE, OR LOSSES OR
EXPENSES RESULTING FROM ANY DEFECTIVE
PRODUCT OR THE USE OF ANY PRODUCT.
NO ORAL OR WRITTEN INFORMATION OR ADVICE
GIVEN BY THE MANUFACTURER, ITS DEALERS,
DISTRIBUTORS, AGENTS OR EMPLOYEES SHALL
CREATE A WARRANTY OR IN ANY WAY INCREASE THE
SCOPE OF THIS WARRANTY.
IMPORTANT - 3
Installation, Relocation and After-Sale Technical Service

(1)
(2)
Installation and Relocation

(a)
Installation at delivery shall not be carried out by the

user. It shall be carried out by our sales
representative or the engineers who have been trained
and qualified for this purpose by us in order to use the
instrument safely and accurately.
(b)
Before installation, the user shall make preparations for

satisfying the installation requirements in accordance
with this instruction manual.
(c)
If relocation becomes necessary after initial installation

(delivery), please contact the dealer from whom you
purchased the instrument or our sales representative.
After-sales Service
(a)
For after-sales service, contact our sales

representative or service office of our sales
representative.
(b)
For service after the warranty period, consult us with

regard to a maintenance and inspection service
contract.
Technical Seminars and Training Courses for Users

We offer technical seminars and training courses at either our or
users facilities to ensure proper and safe operation of the
analytical instrument to its full performance. For further
information, contact our sales representative. (Applicants will be
charged.)
IMPORTANT - 4
Precautions on Disposal of Instrument

(1)
Although at present this instrument does not use materials

that would directly cause environment disruption, the
environmental protection laws and regulations may be
revised or amended. When disposing of this instrument,
check the latest issues of the relevant laws and regulations
or consult your local Hitachi High-Technologies Corporation
sales representative or service office of Hitachi HighTechnologies Corporation sales representative.
(2)
For disposal of waste solution, refer to the instruction manual

for L-8900 high speed amino acid analyzer (main unit).
(1)
The user is responsible for following relevant legal standards

and regulations in the handling, storage and discarding of
chemicals to be used for cleaning this instrument.
(2)
Chemicals shall be handled, stored and discarded as

instructed by respective suppliers.
(3)
Physiologically or biologically active samples are not

applicable to this instrument because of possible infection
with bacteria or viruses.
Other Precautions
Handling of Chemicals
IMPORTANT - 5
SAFETY SUMMARY
General Safety Guidelines
Before using the Model L-8900 high speed amino acid analyzer,
be sure to read the following safety instructions carefully.
The hazard warnings which appear on the warning labels on the
instrument or in the manual have one of the following alert
headings consisting of an alert symbol and signal word DANGER,
WARNING, or CAUTION.
: The alert symbol shown at left precedes
every signal word for hazard warnings,
and appears in safety-related
descriptions in the manual.
To prevent possible hazards or injury, be
sure to follow the safety precautions
preceded by this symbol.
DANGER : Indicates an imminently hazardous
situation which, if not avoided, will result
in death or serious injury.
WARNING : Indicates a potentially hazardous
situation which, if not avoided, will or can
result in death or serious injury.
CAUTION : Indicates a hazardous situation which, if
not avoided, will or can result in minor or
moderate injury, or serious damage to
the product.
CAUTION : Indicates a potentially hazardous
situation which, if not avoided, will or can
result in damage to the product or
property.
In addition to the above, the following signal word is used to
indicate instructions for ensuring proper use of the product.
NOTE: Indicates an instruction for ensuring connect use of
the product and accurate analysis therewith.
SAFETY - 1
SAFETY SUMMARY
Common Safety Precautions
Prior to Use
Before using the instrument, be sure to read this instruction
manual carefully to attain a full understanding of its operations.
Keep the instruction manual handy nearby so it can be referred
to whenever needed.
Be sure to observe the procedures specified in the manual.
Be sure to understand and follow all the safety instructions
given in the manual.
Be sure to observe all the hazard warnings attached to the
instrument or provided in the manual. Failure to do so could
result in personal injury or damage to the instrument.
Be sure to follow all the methods of use instructed in the
manual for proper application of the product.
Absolutely avoid modifying the product, using non-specified
parts, or removing safety devices as it could be hazardous.
Do not perform any operation or action other than described in
the manual.
On occurrence of any trouble in the instrument, notify the
nearest sales representative or service office of Hitachi HighTechnologies Corporation.
When using chemicals for the instrument, be sure to provide
proper ventilation of the room. Inadequate ventilation could
endanger human health.
Keep in mind that the hazard warnings in the manuals or on the
product cannot cover every possible case, as it is impossible to
predict and evaluate all circumstances beforehand. Always be
alert and use your common sense.
SAFETY - 2
SAFETY SUMMARY
Common Safety Precautions (Continued)
In Use
If an abnormality such as unusual noise, odor, fuming or gas
leakage occurs during operation of the instrument, immediately
shut down power supply, close the main gas valve, and take
proper safety measures as required. Then, notify the nearest
Hitachi High-Technologies Corporation sales representative or
service office of Hitachi High-Technologies Corporation sales
representative.
Installation, Maintenance, and Relocation

At the time of delivery, installation of the instrument shall be
carried out by or under supervision of qualified service
personnel of the manufacturer or its authorized service agent
for ensuring safety and high accuracy in operation of the
instrument. It is not permitted for the user to carry out
installation.
After completion of installation, check that all the standard parts
are equipped. If the instrument is made active with any one of
the standard parts not equipped, a failure could occur to result
in a hazardous condition.
If any item is missing or damaged or if you have any question,
notify the installation personnel at site or the nearest Hitachi
High-Technologies Corporation sales representative or service
office of Hitachi High-Technologies Corporation.
The maintenance and checkup procedures to be taken by the
user are only those described in the manual. When taking the
maintenance and checkup procedures described in the manual,
attain a clear understanding of them.
Do not perform other maintenance and checkup procedures to
avoid jeopardizing safety and causing troubles in the instrument.
SAFETY - 3
SAFETY SUMMARY
Common Safety Precautions (Continued)
After installation, do not relocate the instrument. If the
instrument is relocated, vibration or impact to be applied during
relocation could cause a malfunction in the optical components
that have been adjusted precisely.
If any warning/caution label has become illegible due to
deterioration with age or it has been damaged due to any
cause, notify the nearest Hitachi High-Technologies
Corporation sales representative or service office of Hitachi
High-Technologies Corporation for replacement with a new one.
SAFETY - 4
SAFETY SUMMARY
Safety Instructions in This Manual
Shown below are the safety instructions contained in this manual
and their relevant sections on it.
DANGER Indications
The indication
DANGER does not apply to this instrument.
WARNING Indications
The indication
WARNING does not apply to this instrument.
CAUTION Indications
Fatigue due to Long-Hour Operation
If you keep working with the display monitor and keyboard for long
hours, your eyes and body will be fatigued to jeopardize your
health. To prevent this, take a break for about 10 to 15 minutes
per hour during operation.
(Section 2)
SAFETY - 5
SAFETY SUMMARY
NOTICE
Accuracy/Precision of Measured Values
For ensuring reliability of analytical data, measure standard
samples before the start of daily operation and make sure that the
instrument operates normally. In particular, be sure to perform
strict control of a peak size (area/height) and retention time of
each component. If a significant delay occurs in retention time of
any component to make its identification impossible, check the
instrument for liquid leakage.
Data Backup
On occurrence of a system failure or wrong operation, data stored
in the hard disk may be damaged or destroyed. It is therefore
advisable to periodically save acquired data from the hard disk to
external storage media such as floppy disks or magneto-optical
disks. In case of accidental damage to the hard disk, make
backup copies of hard disk files periodically.
Power Interruption
On occurrence of momentary power voltage drop due to power
interruption or lightning, the personal computer may become faulty
or the system software, application software or data may be
damaged.
Turning On/Off Personal Computer
Do not turn off power to the personal computer while the hard disk
or floppy disk drive is active. If power to the personal computer is
turned off while the hard disk or floppy disk is being accessed, the
personal computer may become faulty or data/software stored in it
may be damaged.
Before turning off power to the personal computer, be sure to quit
the AMINOOASYS, EZChrom Elite for Hitachi AAA, and
environment setup software. Then, take the shut-down
procedure using the system software.
Do not install a commercial program onto the personal computer.
Avoid changing the presettings on the personal computer.
SAFETY - 6
SAFETY SUMMARY
NOTICE (Continued)
Protection against Computer Viruses
If any program/data is damaged suddenly or an unexpected
operation/screen is encountered, the personal computer is
suspected of being infected by a computer virus.
Computer viruses are malicious programs that sneak into personal
computers to cause misbehavior or damage to data, and a
program designed to offer protection against computer viruses is
called a vaccine program.
Possible causes of virus infection are:
Downloading a virus-laden program through communication.
Using a floppy disk or other storage medium infected by a
virus.
Note also that once any personal computer is infected by a virus, it
may spread to other computers via communication or storage
medium.
Never use a program or storage medium that is suspected of
containing a virus.
If there is a possibility of virus infection, check for a virus using a
vaccine program. Note, however, that some kinds of vaccine
programs cannot eradicate particular viruses. So, be sure to
make a backup of hard disk files beforehand.
The user is requested to prepare a vaccine program and carry out
virus removal on users own responsibility.
SAFETY - 7
CONTENTS
PREFACE
ABOUT THIS MANUAL
IMPORTANT............................................................................................... IMPORTANT-1
Warranty on Product ................................... IMPORTANT-1
Installation, Relocation and After-Sale
Technical Service........................................ IMPORTANT-4
Technical Seminars and Training Courses
for Users ..................................................... IMPORTANT-4
Precautions on Disposal of Instrument........ IMPORTANT-5
Other Precautions ....................................... IMPORTANT-5
SAFETY SUMMARY....................................................................................SAFETY-1
General Safety Guidelines .............................SAFETY-1
Common Safety Precautions .........................SAFETY-2
Safety Instructions in This Manual .................SAFETY-5
DANGER Indications ...............................SAFETY-5
WARNING Indications .............................SAFETY-5
CAUTION Indications ..............................SAFETY-5
Fatigue due to Long-Hour Operation .......SAFETY-5
NOTICE .........................................................SAFETY-6
1.
FUNCTION............................................................................................................... 1-1
1.1 Construction of L-8900............................................. 1-1
1.2 Setup of L-8900......................................................... 1-5
1.3 Setup of Rack Parameters ....................................... 1-7
1.4 Setup of Column Information .................................. 1-9
1.5 Setup of L-8900 Parameters .................................... 1-15
1.5.1
Column ......................................................... 1-15
1.5.2
Detector ........................................................ 1-16
1.5.3
Reactor Heater ............................................. 1-16
1.5.4
Method Type ................................................. 1-17
1.5.5
Autosampler.................................................. 1-18
1.5.6
Pump ............................................................ 1-19
1.5.7
Auto Generation Button ................................ 1-19
1.5.8
Time Program Table ..................................... 1-19
1.5.9
Calculate Reagent Consumption Screen ...... 1-22
-i-
1.5.10
1.6
1.7
1.8
1.9
1.10
1.11
1.12
1.13
1.14
1.15
1.16
2.
Cautions on Calculation of Reagent

Consumption................................................. 1-23
1.5.11 Auto Generation Screen of RG Process ....... 1-24
1.5.12 Setup of Aux (Auxiliary) Traces..................... 1-28
1.5.13 Setup of Baseline Check............................... 1-29
1.5.14 Setup of Trigger ............................................ 1-30
Instrument Status Screen (L-8900 tab) ................... 1-31
1.6.1
The State of Status Information Display........ 1-32
1.6.2
System Buttons............................................. 1-34
1.6.3
Manual Buttons ............................................. 1-35
1.6.4
Manual Operation Buttons ............................ 1-35
1.6.5
Maintenance Buttons .................................... 1-36
1.6.6
Flow Diagram Icon ........................................ 1-37
1.6.7
Module Information Dialog Box ..................... 1-38
1.6.8
Pump1 Setup Dialog Box .............................. 1-39
1.6.9
Pump2 Setup Dialog Box .............................. 1-40
1.6.10 Autosampler Dialog Box................................ 1-41
1.6.11 Column Oven Dialog Box.............................. 1-42
1.6.12 Reactor Heater Dialog Box ........................... 1-43
1.6.13 W Lamp Dialog Box ...................................... 1-43
1.6.14 Pump Pressure 0 Adjust Dialog Box ............. 1-44
1.6.15 Maintenance Information Dialog Box............. 1-45
1.6.16 Column Information Dialog Box..................... 1-48
Instrument Status Screen (Option tab) ................... 1-51
1.7.1
Status Information Display ............................ 1-51
1.7.2
Pump Maintenance ....................................... 1-52
1.7.3
N2 Gas Valve................................................. 1-56
Pump ON/OFF Dialog Box ....................................... 1-58
Error Message List ................................................... 1-60
Status Transition ...................................................... 1-71
Warming-up Process................................................ 1-72
RG Process ............................................................... 1-75
Washing Process...................................................... 1-76
Automatic-Stop Processing by Module
Operation .................................................................. 1-77
Status Display of Autosampler Tray........................ 1-78
Ingredient Weight Calculation Function ................. 1-79
OPERATION ............................................................................................................ 2-1

2.1 Operational Flow ...................................................... 2-1
2.2 Preoperational Procedure up to Power-On ............ 2-2
2.2.1
Installation of Buffer Solutions and
Ninhydrin Reagent (when installing newly).... 2-2
2.2.2
Installation of Autosampler Washing
Solution......................................................... 2-2
- ii -
2.3
2.4
2.5
2.6
2.7
2.8
Startup of L-8900 ...................................................... 2-3

2.3.1
Power-On of Analyzer ................................... 2-3
2.3.2
Adjustment and Check of N2 Gas
Pressure ....................................................... 2-3
2.3.3
Replacement of Buffer Solution and
Ninhydrin Reagent ........................................ 2-3
2.3.4
Substitution of Ninhydrin Reagent with
N2 Gas .......................................................... 2-4
2.3.5
Substitution of Liquid in Flow Path and
Pump ............................................................ 2-4
Startup of EZChrom Elite for HITACHI .................... 2-5
2.4.1
Startup of Windows XP ............................... 2-5
2.4.2
Startup of EZChrom Elite for Hitachi ............. 2-6
Instrument Configuration......................................... 2-7
2.5.1
Creation of New Instrument .......................... 2-7
2.5.2
Instrument Configuration............................... 2-9
Preoperational Procedure prior to Analysis ........... 2-13
2.6.1
Startup of Instrument Icon............................. 2-13
2.6.2
Setup of Pump1 Flow Rate (purging of
buffer solution) .............................................. 2-14
2.6.3
Attachment of Column .................................. 2-15
2.6.4
Setup of Pump2 Flow Rate (purging of
reaction solution)........................................... 2-16
2.6.5
Setup of Autosampler (deaeration of
syringe) ......................................................... 2-16
2.6.6
Zeroing of Pump Pressure ............................ 2-17
2.6.7
Setting of Standard Sample and Unknown
Sample.......................................................... 2-18
2.6.8
Check Points before Start (routine check
items)............................................................ 2-19
Creation of Method................................................... 2-20
2.7.1
Setup of Instrument Conditions..................... 2-20
2.7.2
Calculation of Reagent Consumption............ 2-22
2.7.3
Saving of Method .......................................... 2-23
2.7.4
Creation and Saving of RG Process
Method.......................................................... 2-23
2.7.5
Editing and Saving of Warm-up Process....... 2-25
Sequence Run (preliminary analysis) ..................... 2-27
2.8.1
Creation and Saving of Sequence Table....... 2-27
2.8.2
Execution of Warm-up and Baseline
Check ........................................................... 2-33
2.8.3
Execution of Sequence Run.......................... 2-35
- iii -
2.9
2.10
2.11
2.12
2.13
2.14
2.15
Waveform Processing and Peak Identification ...... 2-37

2.9.1
Waveform Processing while Viewing
Chromatogram.............................................. 2-37
2.9.2
Peak Identification......................................... 2-43
Generation of Report................................................ 2-50
2.10.1 Report Setup................................................. 2-50
Sequence Run (execution of continuous
analysis) .................................................................... 2-52
2.11.1 Creation and Saving of Sequence Table....... 2-52
2.11.2 Execution of Warm-up .................................. 2-58
2.11.3 Execution of Sequence Run (addition of
Run Queue) .................................................. 2-59
Sequence Processing .............................................. 2-61
2.12.1 Check of Working Curve............................... 2-61
2.12.2 Sequence Processing ................................... 2-61
2.12.3 Creation of Sequence Table for
Reprocessing................................................ 2-62
2.12.4 Execution of Sequence Run.......................... 2-66
Termination of EZChrom Elite for HITACHI ............ 2-68
2.13.1 Termination of EZChrom Elite....................... 2-68
2.13.2 Termination of Windows XP ........................ 2-69

2.13.3 Forced Termination of Program at
Occurrence of Trouble .................................. 2-70
Shutdown of L-8900 ................................................. 2-70
Precautions on Measurements................................ 2-71
- iv -
1.
FUNCTION
1.1
Construction of L-8900 .......................................................................................... 1-1
1.2
Setup of L-8900 ..................................................................................................... 1-5
1.3
Setup of Rack Parameters..................................................................................... 1-7
1.4
Setup of Column Information ................................................................................. 1-9
1.5
Setup of L-8900 Parameters.................................................................................. 1-15

1.5.1
Column .................................................................................................... 1-15
1.5.2
Detector ................................................................................................... 1-16
1.5.3
Reactor Heater......................................................................................... 1-16
1.5.4
Method Type ............................................................................................ 1-17
1.5.5
Autosampler............................................................................................. 1-18
1.5.6
Pump ....................................................................................................... 1-19
1.5.7
Auto Generation Button............................................................................ 1-19
1.5.8
Time Program Table ................................................................................ 1-19
1.5.9
Calculate Reagent Consumption Screen.................................................. 1-22
1.5.10 Cautions on Calculation of Reagent Consumption ................................... 1-23
1.5.11 Auto Generation Screen of RG Process................................................... 1-24
1.5.12 Setup of Aux (Auxiliary) Traces ................................................................ 1-28
1.5.13 Setup of Baseline Check.......................................................................... 1-29
1.5.14 Setup of Trigger ....................................................................................... 1-30
1.6
Instrument Status Screen (L-8900 tab) .................................................................. 1-31

1.6.1
The State of Status Information Display ................................................... 1-32
1.6.2
System Buttons........................................................................................ 1-34
1.6.3
Manual Buttons ........................................................................................ 1-35
1.6.4
Manual Operation Buttons........................................................................ 1-35
1.6.5
Maintenance Buttons ............................................................................... 1-36
1.6.6
Flow Diagram Icon ................................................................................... 1-37
1.6.7
Module Information Dialog Box ................................................................ 1-38
1.6.8
Pump1 Setup Dialog Box ......................................................................... 1-39
1.6.9
Pump2 Setup Dialog Box ......................................................................... 1-40
1.6.10
1.6.11
1.6.12
1.6.13
1.6.14
1.6.15
1.6.16
Autosampler Dialog Box........................................................................... 1-41

Column Oven Dialog Box ......................................................................... 1-42
Reactor Heater Dialog Box....................................................................... 1-43
W Lamp Dialog Box ................................................................................. 1-43
Pump Pressure 0 Adjust Dialog Box ........................................................ 1-44
Maintenance Information Dialog Box........................................................ 1-45
Column Information Dialog Box................................................................ 1-48
1.7
Instrument Status Screen (Option tab)................................................................... 1-51

1.7.1
Status Information Display ....................................................................... 1-51
1.7.2
Pump Maintenance .................................................................................. 1-52
1.7.3
N2 Gas Valve ............................................................................................ 1-56
1.8
Pump ON/OFF Dialog Box..................................................................................... 1-58
1.9
Error Message List................................................................................................. 1-60
1.10 Status Transition.................................................................................................... 1-71

1.11 Warming-up Process ............................................................................................. 1-72
1.12 RG Process ........................................................................................................... 1-75
1.13 Washing Process................................................................................................... 1-76
1.14 Automatic-Stop Processing by Module Operation.................................................. 1-77
1.15 Status Display of Autosampler Tray....................................................................... 1-78
1.16 Ingredient Weight Calculation Function ................................................................. 1-79
1.1
1.
1.1
FUNCTION
Construction of L-8900
The hardware (Hitachi L-8900 type high-speed amino acid
analyzer) of EZChrom Elite for Hitachi AAA is called Instrument
in this instruction manual.
With the following procedures, the instrument that uses L-8900
module is set up.
(1)
Select File/New/Instrument in the main menu.

A new instrument icon is created.
(2)
An instrument can be renamed:

Right-click on the instrument icon, select Rename and input
a new name.
1-1
1.1
(3)
Right-click on the instrument icon.

Select Configure/Instrument.
The dialog box of Instrument Configuration is displayed.
(4)
Click the drop-down list of Instrument type.

Select Hitachi L-8900 Series AAA.
The following message is displayed:
Change of the instrument type deletes the setup of all
devices
Click the OK button.
(5)
Click Configure.
A dialog box of Hitachi L-8900 Series AAA is opened.
The icon of L-8900 is displayed in the Available modules
box.
1-2
1.1
(6)
Move L-8900 icon to Configured modules from the

Available modules:
Select L-8900 icon.
Click the green arrow [] button (or double-click the L-8900
icon).
To delete a module from Configured modules:
Select the module icon and click the red arrow [] button.
NOTE: The ? mark is attached to the L-8900 icon

immediately after moving to the Configured
modules box. This indicates that the module is not
set up. Complete a setup according to the following
procedures.
(7)
Click the Auto Configuration button.
(8)
1-3
1.1
(9)
Right-click the L-8900 icon in the Configured modules box.

Select Open (or double-click the icon).
A Hitachi L-8900 setup dialog is displayed.
(10) Click the Options button.

The use of the option software of System Suitability and
Baseline Check are settable.
System Suitability
This is an option for calculating the parameters related to
column performance, such as theoretical plate number and
symmetry coefficient.
This is checked automatically when L-8900 module is set as
Configured modules.
Baseline Check
This is an option for checking the baseline noise and drift.
It is an effective function for stability check of the system.
This is checked automatically when L-8900 module is set as
Configured modules.
1-4
1.2
1.2
Setup of L-8900
Right-click the L-8900 icon.
Select Open.
A Hitachi L-8900 Configuration dialog box is displayed.
Serial Number
Set up Serial No. (serial number) of L-8900 to be connected.
Pressure Unit
Select the pressure unit to be used.
Available units : bar, psi, MPa, kgf/cm2
Default unit
: MPa
Rack Type
Usually, Single is chosen.
Select Multi when using a micro plate rack.
Rack A, B, C
Select the rack name to be used.
Selection can be made from 16 kinds of racks: STD(default value),
Cool100, Rack3 to 10, MP96-A, MP96-B, MP96-C, MP384-A,
MP384-B, MP384-C.
If a Multi-Rack is chosen, a maximum of three micro plates can
be set.
A rack is chosen for each of back (A), center (B) and front (C) of
the instrument.
Click the Racks Parameter button (
) and a Rack Parameter
dialog box is opened. Input the parameter of the selected rack.
(Racks of B and C are displayed when Multi is chosen as a rack
type.)
1-5
1.2
Setup of L-8900
Cooling Rack
This check box is automatically set to ON when the cooling rack is
connected to the autosampler.
Monitoring (temperature monitoring) of a cooling rack is performed.
Cooling Rack temperature is set as either 4 C or 10 C.
(This item is masked when the Cooling Rack is not connected.)
Column Maintenance
The Column Maintenance dialog box that permits correcting
column information (Guard column, Main column, AmmoniaFilter
column, Reaction column) is opened.
Option
This check box is automatically set to ON when the optional pump
purge valve or load sensor is connected. (This item is masked
when neither pump purge valve nor load sensor is connected.)
1-6
1.3
1.3
Setup of Rack Parameters

Click the Rack Parameter button.
The Rack Parameter dialog box is displayed.
Input the rack parameters that are set in the autosampler.
Rack Name
The Rack Name selected in the Autosampler Setup dialog box is
displayed.
X Vials (Nx)
Input the number of vials available in each row of the sample rack.
The input range is from 1 to 40.
Y Vials (Ny)
Input the number of vials available in each column of the sample
rack. The input range is from 1 to 40.
First X Position (mm)
Input the distance (mm) in the X-axis direction from the home
point to the center of the closest vial in the rack.
The input range is from 14.5 to 139.0.
First Y Position (mm)
Input the distance (mm) in the Y-axis direction from the home
point to the center of the closest vial in the rack.
1-7
1.3
Setup of Rack Parameters
Last X Position (mm)

Input the distance (mm) in the X-axis direction from the home
point to the center of the farthest vial in the rack.
Last Y Position (mm)
Input the distance (mm) in the Y-axis direction from the home
point to the center of the farthest vial in the rack.
Depth (mm)
Input the distance (mm) by which the needle descends from its
home point prior to beginning to aspirate a sample.
The range is from 0.0 to 57.0.
Pattern
Select the order and direction of sampling, Row by Row 1 to 4, or
Column by Column 1 to 4.
Update
Press this button to save the changes to the existing parameters or
to create a new rack. The Rack Parameter dialog will close.
Cancel
Press this button to close the dialog without saving the changed
parameters.
1-8
1.4
1.4
Setup of Column Information

The Column Maintenance (Guard column, Main column,
AmmoniaFilter column and Reaction column) dialog box is
displayed by clicking the Column Maintenance button on Hitachi
L-8900 Configuration dialog box.
Guard Column list

Displays the registered information of a Guard column. One line
currently displayed is selectable by single click (selection of two or
more lines is impossible).
Guard Column Add
Displays the Column Registration dialog box that registers a
Guard column newly.
Guard Column Change
Displays the Column Registration dialog box that updates Guard
column information currently selected.
Guard Column Delete
Deletes the Guard column information currently selected.
1-9
1.4
Main Column list

Displays the Main column registered information.
One line currently displayed is selectable by single click (selection
of two or more lines is impossible).
Main Column Add
Main column newly.
Main Column Change
Displays the Column Registration dialog box that updates Main
column information currently selected.
Main Column Delete
Deletes the Main column information currently selected.
AmmoniaFilter Column list
Displays the AmmoniaFilter column registered information.
AmmoniaFilter Column Add
Displays the Column Registration dialog box that registers an
AmmoniaFilter column newly.
AmmoniaFilter Column Change
Displays the Column Registration dialog box that updates
AmmoniaFilter column information currently selected.
AmmoniaFilter Column Delete
Deletes the AmmoniaFilter column information currently selected.
Reaction Column list
Displays the Reaction column registered information.
Reaction Column Add
Reaction column newly.
1 - 10
1.4
Reaction Column Change

Displays the Column Registration dialog box that updates
Reaction column information currently selected.
Reaction Column Delete
Deletes the Reaction column information currently selected.
OK
Saves the settings and closes the dialog box.
Cancel
Closes the dialog box without saving the changes.
1 - 11
1.4

A Column Registration dialog box is displayed by the following
procedures:
Click the Add button in the Column Maintenance dialog box.
Or, click the Change button after selecting one line in any list box.
Mode
Displays the type of column that is currently selected.
(Guard column, Main column, AmmoniaFilter column or Reaction
column)
Model Name
Set the type name of the column.
1 - 12
1.4
ID Number
Set the ID Number of the column. A unique ID is set for every
kind of column. A multiple setup of registered ID numbers cannot
be performed. (Setting possible when adding, only indication
provided when updating.)
Count
Set the number of times of column use (number of analyses).
Input 0 (zero) and reset the number of times of column use when
a column is changed to a new one (Guard column, Main column
and AmmoniaFilter column only).
Input range: 0 to 9999
Changed Date
Set the exchange date of a Reaction column (Reaction column
only).
Std Replacement Injs
Set the standard number of times of column use (number of
analyses) (Guard column, Main column, AmmoniaFilter column).
Number of standard uses (times):
Guard column
: 200
Main column
: 500
AmmoniaFilter column : 5000
Std Time (y)
Set the standard lifetime (years) of a Reaction column.
Standard lifetime: 2 years.
OK
Saves the settings and closes the dialog box.
Cancel
Closes the dialog box without saving the changes.
1 - 13
1.4
Column Information Delete

Select one line of any list box in the Column Maintenance dialog
box. And click the Delete button. A Column Delete dialog box
is displayed.
OK
Deletes the selected column information and closes the dialog box.
Cancel
Closes the dialog box without deleting the selected column
information.
1 - 14
1.5.1
1.5
Setup of L-8900 Parameters

After the setup of L-8900 configuration, Methods and Sequences
are created and an analysis can be started. Refer to EZChrom
Elite User Guide for details of the sample analyses and the
creation of Methods or Sequences.
The setting method of the Method Parameter of L-8900 is
indicated in the following. Display the instrument condition
screen. Open the instrument icon from EZChrom. And select
Create/Change of Method from the Instrument Wizard screen.
NOTE: Be sure to select File/Method/Save As (or Save)
after the end of Method editing. And the Method
will be saved.
1.5.1 Column
Guard Column
Select the Guard Column (option) to be used.
Guard column wont be used.
Main Column
Select the Main Column to be used.
AmmoniaFilter Column
Select the AmmoniaFilter Column to be used.
Reaction Column
Select the Reaction Column to be used.
1 - 15
Select blank if a
1.5
1.5.2 Detector
Ch1 Stop Time (min)

Set the data acquisition time of the Ch1 Detector.
Input range: 1.0 to 600.0 min
Ch2 Stop Time (min)
Set the data acquisition time of the Ch2 Detector.
Input range: 1.0 to 600.0 min
Sampling Period (ms)
Set the data acquisition interval in milliseconds.
(Common to Ch1 and Ch2)
As for acquisition time (Ch1, Ch2) of the detector, the input range
changes with setup of Sampling Period. The upper and lower
limits of input are as follows.
Sampling Period (ms)
100
200
400
800
1600
3200
Acquistion Time (min) (Stop Time)

Lower Limit
Upper Limit
1.0
1.0
1.0
1.0
1.0
1.0
120.0
240.0
480.0
600.0
600.0
600.0
Auto Zero Before Injection

Select the Auto-Zero setup of a detector signal value when
starting data acquisition (common to Ch1 and Ch2).
1.5.3 Reactor Heater
Temp (C)
Set the temperature of the Reactor Heater.
Input range: 50 to 140 C
NOTES: 1.
2.
Use the Method with which the setup

temperature of the Reactor Heater is the same
when the Multi-Method is used.
The contents of the method can be set to the
instrument unit when the Download Method is
performed.
Note that even if the Reactor Heater is set at
OFF manually, it will be turned ON upon
executing the Download Method.
1 - 16
1.5.4
1.5.4 Method Type
Select the Method Type.

Normal
The method for usual analysis
For Standby
The method for warming-up (standby) processes
For RG
The method for RG (regeneration) processes
RG Data Sampling
Specify whether or not to execute data acquisition when method
type is RG.
The acquisition time of the detector is compared with the last step
time of the time program table, and the larger one is the Cycle
Time.
Data acquisition in analysis is the setup value of the acquisition
time of the detector.
However, in the Standby method and RG method (without data
acquisition), data acquisition is not performed irrespective of the
setting.
NOTE: If the Auto Generation button is clicked after
Normal Method creation, RG Method will be created
automatically. In the usual analysis, these Methods
(Normal Method and RG Method) are used in a pair.
Therefore, save by using a file name with which it is
easy to identify these methods as a pair.
[e.g.] Normal method
RG method
1 - 17
L-8900-PH
L-8900-PH (RG)
1.5
1.5.5 Autosampler
Syringe Volume (L)

Set the syringe volume to be used.
Input range: 100 (option), 500 L
Syringe Speed
Set the syringe speed.
Input range: 1 (low speed) to 5 (high speed)
Needle Down Speed
Set needle descending speed.
Input range: Low speed, High speed
Wash Port Wash Strokes
Set the number of washings for the syringe.
Wash Port Wash Speed
Set the washing speed for the syringe.
Input range: 1 (low speed) to 5 (high speed)
Needle Wash Time (s)
Set the washing time for the outer wall of the needle.
Input range: 0 to 30 s.
Time Injection With Pump (PASS)
Specify whether sample injection timing will be synchronized with
the pump.
Sense Missing Vial
Set whether to detect the existence of a vial at the time of sample
injection.
Plunger Wash Volume (L)
Set liquid volume for washing the pump plunger after the end of
measurement.
Input range: 0 to 5000 L
1 - 18
1.5.6
1.5.6 Pump
Pressure Limit Pump1 (Min, Max)

Set the pressure limiter (minimum and maximum pressures) of
pump1.
Input range:
Minimum value : 0.0 - 29.9 (MPa), 0 - 4338 (psi),
2
0 - 305 (kgf/cm ), 0 - 299 (bar)
Maximum value : 0.1 - 30.0 (MPa), 14 - 4352 (psi),
2
1 - 306 (kgf/cm ), 1 - 300(bar)
Pressure Limit Pump2 (Min, Max)
Set the pressure limiter (minimum and maximum pressures) of
pump2.
Input range:
Minimum value : 0.0 - 29.9 (MPa), 0 - 4338 (psi),
2
0 - 305 (kgf/cm ), 0 - 299 (bar)
Maximum value : 0.1 - 30.0 (MPa), 14 - 4352 (psi),
2
1 - 306 (kgf/cm ), 1 - 300(bar)
Pump1 (B1, B2, B3, B4, B5, B6)
Set the names of solvents (buffer solutions) B1, B2, B3, B4, B5, B6.
Input range: 30 alphanumeric characters
Pump2 (R1, R2, R3)
Set the names of solvents (reagents) R1, R2, R3.
Input range: 30 alphanumeric characters
)
Calculate Reagent Consumption button (
Displays Calculate Reagent Consumption dialog box.
1.5.7 Auto Generation

Button
Displays the auto-generation dialog box of RG process from the

current Time Program Table.
1.5.8 Time Program

Table
Time (min)
Input the time for change of the following items:
Mixture ratio of Eluent (Buffer Solution)/Mixture ratio of Reaction
liquid/Flow rate/Column oven temperature.
The maximum input steps are 92 lines.
Input range: 0.0 to 600.0 min.
1 - 19
1.5
Mixing Ratio of Eluent (B1, B2, B3, B4, B5, B6)

Input the Eluent (Buffer Solution) mixture ratio of Pump1.
Input so that the sum total of 6 liquids may become 100%.
A numerical input is unnecessary for B1 cell. After inputting B2 to
B6, the value of B1 is automatically set so that the sum total of
6 liquids may become 100%.
Input range: 0 to 100%
Flow Rate (Pump1)
Input a flow rate of Pump1.
Input range: 0.000 to 0.999 mL/min.
Column Oven Temperature
Input the temperature of column oven.
Input range: 20 to 85 C
Mixing Ratio of Reaction Liquid (R1, R2, R3)
Input the reaction liquid mixture ratio of Pump2.
Input so that the sum total of 3 liquids may become 100%.
A numerical input is unnecessary for R1 cell. After inputting R2
and R3, the value of R1 is automatically set so that the sum total
of 3 liquids may become 100%.
Input range: 0 to 100%
Flow Rate (Pump2)
Input a flow rate of Pump2.
Input range:
0.000 to 0.999 mL/min.
NOTE: Although the flow rate of pump2 can be set at
0 mL/min, generally do not set a value of 0 mL/min.
When not flowing R1 and R2, set the flow rate of
pump2,and set R3 as 100%.
When creating or modifying the Time Program Table, the operable

menu items in the pop-up menu displayed by right clicking the
mouse are as follows.
Parameters
Time
Mixture ratio
Column oven
temperature
Flow rate
OK:
Cut
Copy
Paste
Delete
OK
OK
NG
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
OK
Operation is possible
1 - 20
NG:
Operation is impossible
1.5.8
In addition, Copy and Paste are available only between the same
kinds of data. For example, it cannot be pasted to the Column
Oven Temperature after mixture ratio of Eluent is copied.
Operation of cell and handling of data are as follows.
Parameters
Operation
Time
Delete
Mixture ratio
of Eluent
Cut
Copy
Paste
Delete
Column
oven
temperature
Cut
Copy
Paste
Delete
Flow rate
Cut
Copy
Paste
Delete
1 - 21
Data Handling
All parameters of the
line containing the
cell concerned.
All data of the eluent
of the line containing
the cell concerned.
the cell concerned.
the cell concerned.
Only the cell
concerned
Only the cell
concerned
Only the cell
concerned
Only the cell
concerned
Only the cell
concerned
Only the cell
concerned
Only the cell
concerned
Only the cell
concerned
Only the cell
concerned
Note
The first line can
not be deleted.
1.5
1.5.9 Calculate
Reagent
Consumption
Screen
Click the Calculate Reagent Consumption button (

) in the
Instrument Setup screen. A Calculate Reagent Consumption
dialog box appears.
This is a function to estimate, after creating and saving the
analysis method, the amount of reagent to be consumed in
analysis using that method.
Injection Counts
Specify the injection count.
Pump1 (B1, B2, B3, B4, B5, B6)
Displays the result of consumption amount calculation for each
eluent (B1, B2, B3, B4, B5, B6).
Pump2 (R1, R2, R3)
Displays the result of consumption amount calculation for each
reaction liquid (R1, R2, R3).
Effluent
Displays the calculation result of the total amount of waste fluid
(B1 to B6, R1 to R3).
Calculation
Performs reagent consumption calculation.
Close
Closes the Calculate Reagent Consumption dialog box.
1 - 22
1.5.10
1.5.10 Cautions on
Calculation of
Reagent
Consumption
(1)
When the amount of reagent consumption is smaller than 1,

revalue the number below a decimal point, regard it as 1, and
display in the unit of 1 mL.
(2)
The amount of reagent consumption calculated does not

include the amount of consumption required by the RG
process. Display RG method, use the Amount Calculation
button of reagent consumption, calculate the amount of
consumption that is required for RG process, and add.
(3)

include the amount of consumption required by the warmingup process.
Display Standby method, use the Amount Calculation
button of reagent consumption, calculate the amount of
consumption that is required for warming-up process, and
add.
(4)

include the amount of consumption required by the baseline
check. When you perform a baseline check, calculate
separately in consideration of operation time.
(5)

include the amount of consumption of washing liquid (C1).
Add: Number of sample injections 1 mL
(6)
The total amount of waste fluid calculated does not include

the amount of waste fluid from washing liquid (C1).
Add: Number of sample injections 1 mL
(7)
The maximum amount of reagent consumption that can be

calculated and displayed is 9999 mL(s).
Since the display will be 9999 mL even if the calculation
result exceeds 10000 mL, calculate separately in such case.
1 - 23
1.5
1.5.11 Auto
Generation
Screen of RG
Process
Click the Auto Generation button in the Instrument Setup screen.

The Auto RG dialog box will be displayed.
Auto Generation
According to the auto generation method of the below-mentioned
RG process, RG process is generated automatically.
Manual Generation
The automatic generation method of the below-mentioned RG
process is unused.
If arbitrary time is set and the process passes the set time, it will
be generated automatically as an RG process.
RG Data Sampling
Specify whether RG data will be acquired and saved during
execution of RG process.
OK
Generates RG process automatically and closes the dialog box.
1 - 24
1.5.11
NOTE: After clicking the Auto Generation button, if the OK

button is clicked in the Auto RG dialog box, the
current editing method will be replaced with the
method of Auto RG process. That is, the original
method will be deleted.
Therefore, before clicking the Auto Generation
button when editing of a method is completed, select
[File/Method/Overwrite or Save As] and be sure to
save the method.
After clicking the Auto Generation button, select
[File/Method/Save As] and save the RG process
method generated automatically under a file name
different from the original method.
The original method is deleted by execution of
[File/Method/Overwrite].
Cancel
Closes the dialog box without generating RG process
automatically.
Automatic Generation Method of RG Process
The RG process is automatically generated by clicking the Auto
Generation button in the Instrument Setup screen according to
the following rules.
(1)
Extracts the step (step of Time: 0 min) of the 1st line from the
time program table under editing, and sets it to the 1st line of
the RG Process Time Program table.
(2)
Searches a step with the following priority from the Time

Program Table under editing, extracts the steps from an
appropriate one to the final one, and sets them from the 2nd
line onward of the RG Process Time Program table.
(a) Count from the last step and extract the step that is the
second one with which the %B5 mixture ratio is one or
more.
(b) When (a) does not exist, count from the last step and
extract the step that is the second one with which
the %B4 mixture ratio is one or more.
(c) When (a) and (b) do not exist, count from the last step
and extract the step that is the second one with which
the %B3 mixture ratio is one or more.
(d) RG process will stop searching when (a), (b) and (c) do
not exist.
1 - 25
1.5
(3)
Changes the time value of the 2nd line to 0.1 min, and if Flow
rate (Pump1, Pump2), Column Oven Temperature, %R1,
%R2 and %R3 are blank, copies from the last step and sets
each one.
(4)
Corrects the time from the 3rd line onward using the same
value as used in the 2nd line.
(5)
Sets the acquisition time(Ch1, Ch2) of the detector as the

time of the last step of RG process time program table
generated automatically. When the RG Data Sampling is
specified in the Auto RG screen, it is set as [the last step
Time - (minus) 1 min].
(6)
Changes Method type to RG Method type.
1 - 26
1.5.11
Time Program Table under Editing

Time
(min)
%B1
%B2
%B3
%B4
%B5
%B6
0.0
100
3.0
100
3.1
100
7.0
100
Pump1
Flow Rate Column
(mL/min) Temp.
0.400
57
60
%R1
%R2
50
50
%R3
0
Pump2
Flow Rate
(mL/min)
0.350
7.1
100
0.200
14.8
100
0.120
14.9
100
29.0
100
29.1
100
32.0
50
50
32.1
100
100
50
50
50
50
33.0
100
33.1
100
34.0
100
34.1
100
70
37.0
37.1
53.0
0.400
100
57
0.350
RG Process Time Generated Automatically

Pump1
Flow Rate Column
(mL/min) Temp.
Time
(min)
%B1
%B2
%B3
%B4
%B5
%B6
0.0
100
0.400
0.200
%R2
57
50
50
0.350
60
50
50
0.120
17.2
50
50
17.3
100
100
50
50
50
50
0.1
100
14.2
100
14.3
100
18.2
100
18.3
100
19.2
100
19.3
100
70
22.2
22.3
38.2
0.400
100
%R3
Pump2
Flow Rate
(mL/min)
%R1
1 - 27
57
0.350
1.5
1.5.12 Setup of Aux

(Auxiliary)
Traces
Select the Aux Traces tab in the Instrument Setup screen.

Select ON or OFF of the pressure monitor of Pump1 and Pump2,
and the temperature monitor of the Column Oven.
By putting check marks at the items to be monitored, the pressure

and temperature can be monitored during the measurement.
The monitored profile can be saved and printed out together with
the chromatographic results after the measurement.
1 - 28
1.5.13
1.5.13 Setup of
Baseline
Check
Select the Baseline Check tab in the Instrument Setup screen.

And select whether or not to perform baseline check.
To pass, the baseline must meet the test criteria for

Set a time to perform baseline check.
Stop checking baseline if conditions are not met after
Set a continuous time of baseline check, if the result of baseline
check was not within the allowable range.
Noise test method
Specify the calculation method used in noise test.
rms
: Calculation method of U.S. Pharmacopoeia

(USP)
ASTM : Calculation method of German
Pharmacopoeia (DAB) and Japanese
Pharmacopoeia (JP)
The allowable range of noise test and drift test is input using the
unit of count.
Conversion value : 1 V = 4 counts
[e.g.]
: 15 V = 60 counts
NOTE: If the baseline check setup is turned ON, a check will
be automatically performed before the start of
analysis. If the threshold value is exceeded and the
result is NG, operation is determined by setup of the
sequence table action Processing at the time of
NG.
1 - 29
1.5
1.5.14 Setup of Trigger
Select the Trigger tab from the Instrument Setup screen.

The trigger tab sets up the start method of instrument.
Select External always for the L-8900.

Available settings: None/Manual/External
NOTE: When starting the instrument without performing
injection: Do not set None. Select External and
set the injection value of the sequence table as 0.
1 - 30
1.6
1.6
Instrument Status Screen (L-8900 tab)

Select Control/Instrument Status from the main menu.
The Instrument Status Screen is displayed.
Monitoring and manual operation can be performed referring to
status information.
1 - 31
1.6
1.6.1 The State of

Status
Information
Display
System Status
Displays the state of instrument.
Display
Uninitialized
Initialized
Idle
Busy
Error
Preview Run
Running (Stand-by)
Running (Baseline)
Running (RG)
Running (Wash)
Running (Injectin Wait)
Running (Setting)
Running (Acquisition
Data)
Running (Device Wait)
Set Parameters
Instrument (control) State

Unable to download (OFF-Line)
Downloading
Idle Standby (ON-Line)
Instrument state is changing
Instrument has incurred error (including
communication error)
Preview running
Warming-up
Baseline test running
RG process running
Wash process running
Autosampler injection waiting
Setting test parameters
Test running
Waiting to finish
Parameter transmission under way
Run Time
The progress time of the current analysis method (Warming-up
process, RG process, and Washing process are included) is
displayed in the unit of 0.1 min.
Cycle Time
Displays the end time of the analysis method currently executing.
(Warming-up process, RG process, and Washing process are
included).
Pump1 (B1, B2, B3, B4, B5, B6)
Displays the mixture ratio of solvents of Pump1.
Pump1 Mode
Displays the mode (control state) of Pump1.
(State: OFF, Constant Flow, Purge, Constant Press, C1 Flow)
Pump1 Flow
Displays the flow rate of Pump1.
1 - 32
1.6.1
Pump1 Pressure
Displays the pressure of Pump1.
Pump2 (R1, R2, R3)
Displays the mixture ratio of reaction liquids of Pump2.
Pump2 Mode
Display the mode (control state) of Pump2.
(State: OFF, Constant Flow, Purge)
Pump2 Flow
Displays the flow rate of Pump2.
Pump2 Pressure
Displays the pressure of Pump2.
Column Temp
Displays the present temperature of the Column Oven.
Reactor Temp
Displays the present temperature of the Reactor.
Autosampler Vial No.
Displays the number of vial under measurement.
Autosampler Inj Volume
Displays the amount of sample injection under measurement.
Autosampler Temp
Displays the present temperature of the Cooling Unit of the
Autosampler.
(This is not displayed when there is no cooling unit.)
Detector Channel1 Data
Displays the signal value of detector Ch1.
The signal value is indicated in counts.
Conversion value:
1 V =
4 counts
[e.g.]
: 15000 V = 60000 counts
Detector Channel2 Data
Displays the signal value of detector Ch2.
The signal value is indicated in counts.
Conversion value :
1 V =
4 counts
[e.g.]
: 15000 V = 60000 counts
1 - 33
1.6
1.6.2 System Buttons
Connect button
Download is performed and PC and L-8900 are changed to the
connection state (On-Line state). This is operable when the
instrument is in the Off-Line state only. It becomes unusable
after connection.
Disconnect button
Click the Disconnect button in the Instrument Status screen.
The Disconnect Dialog box will be displayed. When the PC and
the L-8900 are connected (On-Line state), it is operable only if
Pump1 and Pump2 have stopped together.
If the OK button is clicked, connection of the PC and L-8900 will

be canceled and it will change to the disconnected state (Off-Line
state).
NOTE: Prior to closing the Instrument Status screen or the
EZChrom Elite for Hitachi program, be sure to click
the Disconnect button and set up the Off-Line state.
Closing cannot be done in the On-Line state.
Module Information button

Displays the Module Information dialog box with which the
connection state of each module can be checked.
Washing Stop button
Click the Washing Stop button in the Instrument Status screen,
and the Washing Stop Dialog box will be displayed. It is operable
only during the washing process.
Click the OK button, and washing process will be forced to

terminate.
1 - 34
1.6.3
1.6.3 Manual Buttons
Manual ON/OFF Pump1 SW button

Controls ON/OFF of pump1 manually.
Manual ON/OFF Pump2 SW button
Controls ON/OFF of pump2 manually.
1.6.4 Manual
Operation
Buttons
Manual Operation Pump1 button

Displays the Pump1 setup dialog box that configures the flow rate,
the pressure and the mixture ratio of Pump1.
Manual Operation Pump2 button

Displays the Pump2 setup dialog box that configures the flow rate,
the pressure and the mixture ratio of Pump2.
Manual Operation Autosampler button
Displays the Autosampler dialog box that configures the syringe
washing and the pump washing, etc.
Manual Operation Column Oven button
Displays the Column Oven dialog box that configures the ON/OFF
setup and the temperature setup of the column oven.
Manual Operation Reactor Heater button
Displays the Reactor dialog box that configures the ON/OFF setup
of the reactor heater.
Manual Operation W Lamp button
Displays the Lamp dialog box that configures the ON/OFF setup of
the lamp.
1 - 35
1.6
1.6.5 Maintenance
Buttons
Maintenance Pump Pressure 0 Adjust button

Displays the Pump Pressure 0 Adjustment dialog box that resets
the pressure of Pump 1 and 2 to 0.
Maintenance Information Reset button
Displays the Maintenance Information Reset dialog box that
checks the maintenance information (periodic maintenance parts)
memorized by instrument.
Maintenance Column Information button
Displays the Maintenance Column Information dialog box that
checks the maintenance information (column) memorized by
instrument.
1 - 36
1.6.6
1.6.6 Flow Diagram

Icon
The operation state of each module can be checked in the flow

diagram on the right-hand side of the Instrument Status screen.
The modules for which the state check can be performed and the
details are as follows.
Pump1, 2
Background color is : Gray : the Pump is in OFF state.

: Yellow : the Pump is in ON state.
Column Oven
Background color is : Gray : the Column oven is in OFF state.

: Yellow : the Column oven is in ON state.
Reactor Heater
Background color is : Gray
: the Reactor heater is in OFF

state.
: Yellow : the Reactor heater is in ON
state.
Detector Lamp
Background color is : Gray
: the Detector lamp is in OFF

state.
: Yellow : the Detector lamp is in ON state.
1 - 37
1.6
1.6.7 Module
Information
Dialog Box
Click the Module Information button in the Instrument Status

screen.
The Module Information dialog box is displayed.
Module
Displays the connection state of each module.
When the options such as Cooling Rack, Load Sensor, Pump
Purge Valve, etc. are not provided, the background of the text box
will be displayed in gray.
Close
Closes the Module Information dialog box.
1 - 38
1.6.8
1.6.8 Pump1 Setup

Dialog Box
Click the Manual Operation Pump1 button in the Instrument

Status screen. The Pump1 Setup dialog box is displayed.
Flow Rate
Controls Pump1 by the Flow rate.
Input the maximum and minimum values of the pressure limiter
range, then the flow rate.
Input range (Pressure):
Minimum value : 0.0 - 29.9 (MPa), 0 - 4338 (psi)
2
0 - 305 (kgf/cm ), 0 - 299 (bar)
Maximum value : 0.1 - 30.0 (MPa), 14 - 4352 (psi)
2
1 - 306 (kgf/cm ), 1 - 300 (bar)
Input range (Flow rate)
: 0.000 - 0.999 (mL/min)
Solvent
Sets up the Solvent mixing ratio of Pump1.
Input range: 0 - 100%
OK
Controls Pump1 according to the set-up conditions.
Cancel
Closes the dialog box without saving the set-up conditions.
1 - 39
1.6
1.6.9 Pump2 Setup

Dialog Box
Click the Manual Operation Pump2 button in the Instrument

Status screen. The Pump2 Setup dialog box is displayed.
Flow Rate
Controls Pump2 by the Flow rate.
Input the maximum and minimum values of the pressure limiter
range, then the flow rate.
Input range (Pressure):
Minimum value : 0.0 - 29.9 (MPa), 0 - 4338 (psi)
2
0 - 305 (kgf/cm ), 0 - 299 (bar)
Maximum value : 0.1 - 30.0 (MPa), 14 - 4352 (psi)
2
1 - 306 (kgf/cm ), 1 - 300 (bar)
Input range (Flow rate)
: 0.000 - 0.999 (mL/min)
Reagent
Sets up the Reagent mixing ratio of Pump2.
Input range: 0 - 100%
OK
Controls Pump2 according to the set-up conditions.
Cancel
1 - 40
1.6.10
1.6.10 Autosampler
Dialog Box
Click the Manual Operation Autosampler button in the

Instrument Status screen. The Autosampler dialog box is
displayed.
Sampler Wash
Washes the washing port and the injection port of the
Autosampler.
Pump Wash
Washes the pump plunger of Pump1 and Pump2.
Home
Resets the Autosampler to the idling state.
Purge Position
The needle of the Autosampler is moved to the position of the
Washing port. (Used at the time of maintenance such as purging
the inside of the Autosampler.)
Needle Change Position
The needle of the Autosampler is moved to the Needle change
position. (Used at the time of maintenance such as needle
change.)
Syringe Change Position
The syringe of the Autosampler is moved to the Syringe change
position. (Used at the time of maintenance such as syringe
change.)
OK
Controls the Autosampler according to the set-up conditions.
Cancel
1 - 41
1.6
1.6.11 Column Oven

Dialog Box
Click the Manual Operation Column Oven button in the

Instrument Status screen. The Column Oven dialog box is
displayed.
Check ON or OFF of temperature control and enter the

temperature of the Column Oven.
Input range: 20 - 85 C
OK
Controls the Column Oven according to the set-up conditions.
Cancel
1 - 42
1.6.12
1.6.12 Reactor Heater

Dialog Box
Click the Manual Operation Reactor Heater button in the

Instrument Status screen. The Reactor Heater dialog box is
displayed.
Check ON or OFF of temperature control of the Reactor Heater

and enter the temperature.
Input range: 50 - 140 C
OK
Controls the Reactor Heater according to the set-up conditions.
Cancel
1.6.13 W Lamp Dialog

Box
Click the Manual Operation Lamp button in the Instrument Status

screen. The W Lamp dialog box is displayed.
Check the ON or OFF of the Detector Lamp.

OK
Controls the Detector Lamp according to the set-up conditions.
Cancel
1 - 43
1.6
1.6.14 Pump
Pressure 0
Adjust Dialog
Box
Click the Maintenance Pump Pressure 0 Adjustment button in

the Instrument Status screen. The Pump Pressure 0 Adjust
dialog box is displayed.
This is used to perform the Pressure 0 Adjustment of Pump1 or

Pump2. Select the pump to be adjusted, Pump1 or Pump2.
OK
Controls the Pump Pressure 0 Adjustment according to the set-up
conditions.
Cancel
NOTE: Perform the Pump Pressure 0 Adjustment after
stopping the pump operation and opening the drain
valve.
1 - 44
1.6.15
1.6.15 Maintenance
Information
Dialog Box
Click the Maintenance Information button in the Instrument

Status screen. The Maintenance Information dialog box is
displayed.
Pump1 Pump Total Flow

Displays the total flow rate, the last reset date and the standard
life of pump1 (Pump seal).
Pump2 Pump Total Flow
Displays the total flow rate, the last reset date and the standard
life of pump2 (Pump seal).
Pump1 Purge Valve Seal
Pump2 Purge Valve Seal
Autosampler Injection Port Seal
Displays the number of injections, the last reset date and the
standard life of injection port seal.
Autosampler Injection Valve Seal
standard life of injection valve seal.
1 - 45
1.6
Autosampler Syringe Seal

standard life of syringe seal.
Autosampler Syringe Valve Seal
standard life of syringe valve seal.
Lamp Used Time
Displays the used hours, the last reset date and the standard life
of lamp.
Lamp ON/OFF Times
Displays the number of lighting operations of the lamp.
Reset button
Click the Reset button in the Maintenance Information dialog box.
The Reset Dialog box is displayed. (The following is an example
of display when the Reset button of Pump1 Pump Total Flow is
clicked.)
When the OK button is clicked the Using value of the displayed

item is reset to 0 and the beginning-of-use date is updated.
Save to Log Dialog button
Click the Save to Log Dialog button in the Maintenance
Information dialog box. The Maintenance Information Save
Close button
Closes the Maintenance Information dialog box.
NOTE: The Standard Life is not a guaranteed value.
It indicates the general lifetime of the part.
These values may change considerably according to
conditions.
1 - 46
1.6.15
Maintenance Information Save dialog box

Click the Save to Log Dialog button in the Maintenance
Information dialog box. The Maintenance Information Save
The information (times of use, exchange date) of each item
currently displayed can be output to a log.
The check box of an item to be output to the log is set at ON.

Check All button
Sets ON the check box of all items.
Clear All button
Sets OFF the check box of all items.
Save to Log button
Outputs the information of the selected item to the log.
(The button can be operated when one or more items are
selected.)
Information is stored in the activity log by the following format.
Maintenance Information Save:
Item Name: <item name> and Use: <A value >< unit> Date: <Date>
Close button
Closes the Maintenance Information Save dialog box without
outputting the information of items to the log.
1 - 47
1.6
1.6.16 Column
Information
Dialog Box
Click the Maintenance Column Information button in the

Instrument Status screen. The Column Information dialog box is
displayed.
Guard Column list

Displays the registered information of the Guard Column.
Main Column list
Displays the registered information of the Main Column.
AmmoniaFilter Column list
Displays the registered information of the Ammonia Filter Column.
Reaction Column list
Displays the registered information of the Reaction Column.
Save to Log Dialog button
Click the Save to Log Dialog button in the Column Information
dialog box. The Column Information Save dialog box is displayed.
1 - 48
1.6.16
Close button
Closes the Column Information dialog box.
NOTE: The Standard Replacement Injections (number of
injections) and the Standard Time (years) are not
guaranteed values. They indicate the general
lifetime of the columns. These values may change
considerably according to conditions.
Column Information Save Dialog box

Click the Save to Log Dialog button in the Column Information
dialog box. The Column Information Save Dialog box is
displayed. This is used to output the currently displayed
information of each column to a log.
Set the check box of the column to be output to the log at ON.
Check All button
Sets the check box of all the columns in the list box at ON.
1 - 49
1.6
Clear All button

Sets the check box of all the columns in the list box at OFF.
Save to Log button
Outputs the information of selected column to a log.
is operable when one or more items are selected.)
(This button
Information is stored in an activity log in the following format.

When Guard/Main/AmmoniaFilter column is selected:
Column Information Save:
Column Name: <column name>, Model Name: <Model>, ID: <ID>,
Count: <Count>, Std Replacement Injs: <Std>
When Reaction column is selected:

Column Information Save:
Column Name: Reaction Column, Model Name: <Model>, ID: <ID>,
Changed Date: <Date>, Std Time (y): <Std>
Close button
Closes the Column Information Save dialog box without writing the
column information to the log.
1 - 50
1.7.1
1.7
Instrument Status Screen (Option tab)

Select Control/Instrument Status from the main menu.
The Instrument Status (Option tab) screen is displayed.
Monitoring and manual operation can be performed referring to
status information.
1.7.1 Status
Information
Display
System Status
Displays the state of instrument (control).
The contents of the display are common to the state display of the
Instrument Status (L-8900 tab) screen (refer to section 1.6.1).
Lamp Energy
Displays the energy value of the detector lamp.
1 - 51
1.7
1.7.2 Pump
Maintenance
Click the Purge button of the Pump1 side.

The Pump1 Purge (maximum flow rate: 0.999 mL/min) operation
Buffer
Delivers buffer solution.
Input the mixture ratio of B1 - B4.
AUX/RG
Delivers auxiliary solution/regenerating solution.
mixture ratio of B5 and B6.
Input the
C1
Delivers washing liquid.
Purge Valve
Start button
Performs purge (maximum flow rate: 0.999 mL/min) of pump1.
NOTE: Open the drain valve manually and then press Start
button.
1 - 52
1.7.2
Cancel button
Cancels the setting and closes the dialog box.
Click the C1 Flow button of the Pump1 side.
The C1 Flow dialog box is displayed.
Set a flow rate and a pressure limit.

(C1) into the Pump1 side flow path.
And deliver washing liquid
Start button
Starts washing liquid (C1) delivery from Pump1.
Cancel button
NOTE: When you deliver washing liquid (C1), set a flow rate
while monitoring the pressure display of Pump1 side.
Since pump pressure rises higher when delivering
washing liquid than when delivering buffer solution,
be careful about breakage or degradation of a
column.
1 - 53
1.7
Click the Constant Pressure Flow button of the Pump1 side.

The Constant Pressure Flow dialog box is displayed.
Liquid is delivered while varying flow rate automatically so as to

attain the set pressure, (This function is used at column packing,
etc.)
Start button
Starts liquid delivery from Pump1 in the Constant Pressure mode.
Cancel button
NOTE: Eluent usable in Constant-Pressure Delivery mode is
fixed at B1. To deliver an eluent other than B1,
reset the target eluent into the position of B1.
1 - 54
1.7.2
Click the Purge button of the Pump2 side.

The Pump2 Purge (maximum flow rate: 0.999 mL/min) dialog box
is displayed.
Mixing Ratio
Delivers Reaction Liquid.
Input the mixture ratio of R1 - R3.
Purge Valve
Start button
Performs purge (maximum flow rate: 0.999 mL/min) of pump2.
NOTE: Open the drain valve manually and then press Start
button.
Cancel button
1 - 55
1.7
1.7.3 N2 Gas Valve
Click the Bubbling button.

The Bubbling dialog box is displayed
Bubbling Time
Input an execution time for bubbling.
Start button
N2 gas valve is automatically changed to the BUBBLING side, and
bubbling is started. When the Bubbling Time has passed, N2 gas
valve is returned to ANALYSIS and the bubbling is ended.
Cancel button
Click the Change Valve Position button of the N2 Gas Valve side.
The Valve Position dialog box is displayed.
Allows changing over the N2 gas valve manually.

ANALYSIS : Position for usual analysis
OPEN
: Position for opening N2 gas
BUBBLING : Position for bubbling
1 - 56
1.7.3
Execute button
Changes the N2 gas valve to the specified position.
Cancel button
1 - 57
1.8
1.8
Pump ON/OFF Dialog Box
Pump ON/OFF Dialog Box

To prevent operational errors or failure of instrument in manual
ON/OFF of Pump1/Pump2, the following message is displayed for
confirmation.
(1)
When Pump1 is ON
Press the OK button.

Pump1 will be turned ON.
(2)
When Pump1 and Pump2 are both ON, and Pump1 is turned
OFF independently.
NOTE: Press the OK button.

Pump1 will be turned OFF.
Independent operation of pump2 may cause
blockage of the flowpath with ninhydrin or backflow
of ninhydrin to the main column, etc., and therefore
should be avoided. There is no problem when
Pump2 is set for R3 (5% EtOH) 100%.
1 - 58
1.8
(3)
When Pump1 is OFF, and Pump2 is turned ON

independently.
NOTE: Press the OK button.

Pump2 will be turned ON.
Independent operation of pump2 may cause
blockage of the flowpath with ninhydrin or backflow
of ninhydrin to the main column, etc., and therefore
should be avoided. There is no problem when
Pump2 is set for R3 (5% EtOH) 100%.
1 - 59
1.9
Error Message List
1.9
Error Message List

System errors and each screen of EZChrom Elite for Hitachi AAA,
and errors generated when setting in dialog boxes are shown
below. Take an appropriate action with reference to the error
message.
<Common Errors>
No
Code
Display
20
ROM
21
RAM
22
EEPROM
23
EEPROM-Log
24
EEPROM-Inf
26
BUFFER
OVER-RUN
27
28
NUMBER OF
TIME
PROGRAM
STEPS
TIME
PROGRAM
RECEPTION
10
11
Error Message
"ROM" error occurred in ***.
Turn off the instrument and
perform re-start.
"RAM" error occurred in ***.
perform re-start.
"EEPROM (parameter)" error
occurred in ***. Turn off the
instrument and perform re-start.
"EEPROM (LOG information
part)" error occurred in ***.
perform re-start.
"EEPROM (information)" error
"BUFFER OVER-RUN" error
"ABNORMALITY IN TIME
PROGRAM" error occurred in
***.
"TIME PROGRAM RECEIVING
TIMEOUT" error occurred in ***.
"COMMUNICATION
CONNECTION ERROR"
Turn on power supply of the
instrument again, after checking
for correct connection of the
USB communication cable.
"DEACTIVE ERROR" occurred
in ***.
*** is unconnectable.
1 - 60
Cause of Error/Action to Take

Failure of ROM domain
operation.
Inform service station.
Failure of RAM domain
operation.
Failure of analysis parameter
storage area operation.
Failure of Log information
Failure of instrument parameter
Data transfer to IFB is
impossible during data
acquisition.
The data buffer overflowed.
The number of time program
steps overflowed (91 or more
steps received).
In time program setting mode,
there is no response for
30 seconds or more from CDS.
Abnormality in communication
connection.
NAK0 reception.
1.9
<Column Oven and Reactor Errors>

No
Code
Display
Error Message

Oven control function
suspended by Temperature
MAX LIMIT error.
Contact the service station.
suspended by Temperature MIN
error.
30
TEMPERATURE
MAXIMUM
LIMITER
"TEMPERATURE
MAXIMUM LIMITER" error
occurred in ***.
31
TEMPERATURE
MINIMUM VALUEOvn
32
TEMPERATURE
MAXIMUM VALUE
-Ovn
33
TEMPERATURE
MINIMUM VALUE
-Plt
34
TEMPERATURE
MAXIMUM VALUE
-Plt
38
External Error IN
3E
TEMPERATURE
SPECIFICATION
3F
LAMP
"ABNORMALITY of
TEMPERATURE
MINIMUM VALUE (the
OVEN side)" error
occurred in ***.
"ABNORMALITY of
TEMPERATURE
MAXIMUM VALUE (the
OVEN side)" error
occurred in ***.
"ABNORMALITY of
TEMPERATURE
MINIMUM VALUE (the
PELTIER side)" error
occurred in ***.
"ABNORMALITY of
TEMPERATURE
MAXIMUM VALUE (the
PELTIER side)" error
occurred in ***.
"TEMPERATURE
CONTROL OFF-BY
EXTERNAL ERROR"
occurred in ***.
"TEMPERATURE
SPECIFICATION (time
program)" error occurred in
***.
"LAMP" error occurred.
40
TEMPERATURE
MAXIMUM VALUE
-Ovn
10
41
TEMPERATURE
MAXIMUM VALUE
-Plt
"ABNORMALITY of
TEMPERATURE
MAXIMUM VALUE (the
OVEN side)" error
occurred in ***.
"ABNORMALITY of
TEMPERATURE
MAXIMUM VALUE
(PELTIER side)" error
occurred in ***.
1 - 61

MAX error.
suspended by Temperature MIN
error.
MAX error.
By external signal input, oven
control is suspended and lamp
is switched off.
Failure in lighting or putting out

lamp.
Error occurred in the heater side
temperature maximum value of
the reactor.
Error occurred in the peltier side
temperature maximum value of
the reactor.
1.9
Error Message List
<Autosampler Errors>
No
Code
Display
Error Message
30
RACK LOADER
"RACK LOADER
OPERATION: X MOTOR"
error occurred in ***.
"ARM OPERATION: Y
MOTOR" error occurred in
***.
"NEEDLE OPERATION: Z
MOTOR" error occurred in
***.
"SYRINGE OPERATION:
SYRINGE MOTOR" error
occurred in ***.
"SYRINGE VALVE
OPERATION: SYRINGE
VALVE MOTOR" error
occurred in ***.
"INJECTION VALVE
OPERATION: INJECTION
VALVE MOTOR" error
occurred in ***.
"VIAL SENSOR" error
occurred in ***.
31
ARM
32
NEEDLE
33
SYRINGE
34
SYRINGE VALVE
35
INJECTION
VALVE
36
VIAL SENSOR
37
38
RACK
PARAMETER
SETUP
VIAL NO. SETUP
"RACK PARAMETER
SETUP" error occurred in
***.
"VIAL NO. SETUP
PARAMETER" error
occurred in ***.
10
39
INJECTION
VOLUME SETUP
11
3A
SYRINGE
SPEED SETUP
12
3B
START STEP
SETUP
"INJECTION VOLUME
SETUP PARAMETER" error
occurred in ***.
"SYRINGE SPEED SETUP
PARAMETER" error
occurred in ***.
ST
"1 . STEP SETUP
PARAMETER" error
occurred in ***.
13
3C
WASH SPEED
SETUP
14
3D
EXTERNAL
CONNECTION
MODULE BUSY
"WASH SPEED SETUP

PARAMETER" error
occurred in ***.
"EXTERNAL CONNECTION
MODULE BUSY" error
occurred in ***.
1 - 62

Error of rack loader (X-axis)
operation.
Error of arm (Y-axis) operation.
Error of needle (Z-axis)

operation.
Error of syringe motor
operation.
Error of syringe valve motor
operation.
Error of injection valve motor

operation.
Failure of vial detection.

Failure of rack parameter setup
range.
1) Setting of number of vials
has exceeded the maximum
number in rack parameters
specified in sequential
program.
2) The program has a setting
that fulfills the condition of
"start vial No. end vial
No.".
Setting of the injection volume
has exceeded syringe capacity.
Syringe speed has exceeded
upper limit value of syringe.
A step number that does not
exist in sequence program
control method is set as Start
Step No.
WASH Syringe speed has
exceeded upper limit of syringe.
A connection module is Busy at
the time of start command
reception.
1.9
(contd)
No
Code
Display
Error Message
15
3F
EXTERNAL ERROR
IN
16
40
COOLING RACK
ROM
17
41
COOLING RACK
RAM
18
42
COOLING RACK
EEPROM
19
43
COOLING RACK IS
OFF (UNDER
INITIALIZATION)
20
44
COOLING RACK IS
OFF
21
45
COOLING RACK
COMMUNICATION
IS TIMEOUT
22
46
ABNORMALITY OF
COOLING RACK
TEMPERATURE
"SUSPENDED BY
EXTERNAL ERROR "
occurred in ***.
"COOLING UNIT ROM"
Turn off the instrument
and perform re-start.
"COOLING UNIT RAM"
"COOLING UNIT
EEPROM(parameter)"
"COOLING UNIT
POWER SOURCE IS
OFF (under initialization)"
"COOLING UNIT
POWER SOURCE IS
OFF (after initialization
end)" error occurred in
***.
"COOLING UNIT
COMMUNICATION IS
TIMEOUT" error
occurred in ***.
"ABNORMALITY of
COOLING UNIT
TEMPERATURE" error
occurred in ***.

Failure of operation of ROM

range on rack side.
Failure of operation of RAM

range on rack side.
Failure of operation of EEPROM

range on rack side.
Power supply of cooling unit

was turned off during
initialization.
Power supply of cooling unit

was turned off after initialization
end.
Failure of communication with
the cooling unit.
Error of temperature of the
cooling unit.
<Pump1/Pump2 Errors>
No
Code
30
31
3
4
32
33
Display
PRESSURE
MAXIMUM
LIMITER
PRESSURE
MINIMUM
LIMITER
MOTOR
EXTERNAL
ERROR IN
Error Message
"PRESSURE MAXIMUM
LIMITER" error occurred in ***.
Check for clogging of flow path

system.
"PRESSURE MINIMUM
LIMITER" error occurred in ***.
Check for leakage of flow path

system.
"MOTOR" error occurred in ***.

"SUSPENDED PUMP BY
EXTERNAL ERROR " occurred
in ***.

1 - 63
1.9
Error Message List
<Detector1/Detector2 Errors>
No
Code
Display
12
ADC DATA
READ
30
ADC
CALIBRATION
Error Message
"ADC DATA READ" error
instrument and perform restart.
"ADC CALIBRATION" error
instrument and perform restart.

Unable to read ADC data.
Zero span adjustment

(0.5[V],1[V])
<Extender Board Errors>

No
Code
Display
Error Message
30
N2 GAS CONNECTION
31
WEIGHT SENSOR
CONNECTION
32
PURGE VALVE
CONNECTION
33
PURGE VALVE 1
"N2 GAS BOARD

CONNECTION" error
occurred in ***. Turn off
the instrument and
perform re-start.
" WEIGHT SENSOR
BOARD CONNECTION"
" PURGE VALVE
BOARD CONNECTION"
" PURGE VALVE 1" error
occurred in ***.
34
PURGE VALVE 2
" PURGE VALVE 2" error

occurred in ***.
35
WEIGHT SENSOR
ZERO ADJUSTMENT
36
N2
ELECTROMAGNETIC
VALVE
38
EXTERNAL ERROR IN
39
10
3A
LIQUID LEAK
(AROUND THE
PUMP1)
LIQUID LEAK
(AROUND THE
PUMP2)
" WEIGHT SENSOR

ZERO ADJUSTMENT"
"N2
ELECTROMAGNETIC
VALVE POSITION " error
occurred in ***.
"EXTERNAL ERROR "
occurred in ***.
"LIQUID LEAK
(PUMP1) " error occurred
in ***.
"LIQUID LEAK
(PUMP2) " error occurred
in ***.
1 - 64
Cause of Error/Action to
Take
N2 gas board is
unconnected.
Weight sensor board is

unconnected.
Purge valve board is

unconnected.
Disagreement of specified
position and feedback
position.
Disagreement of a specified
position and feedback
position.
Zero adjustment is out of
range.
After the specified time,
electromagnetic valve
position is still OPEN.
Liquid leak is detected at
pump1.
pump2.
1.9
(contd)
No
Code
Display
Error Message
Cause of Error/Action to
Take
11
3B
3C
"LIQUID LEAK
(AUTOSAMPLER) " error
occurred in ***.
"LIQUID LEAK
(DETECTOR) " error
occurred in ***.

A/S.
12
13
3D
LIQUID LEAK
(AROUND THE
AUTOSAMPLER)
LIQUID LEAK
(AROUND THE
DETECTOR / FLOW
CELL)
LIQUID LEAK
(AROUND THE OVEN /
REACTOR)
"LIQUID LEAK (OVEN) "


oven.

detector.
<Common Errors>
No
Error Message
%1 is invalid. Input %2 - %3.
" " is invalid. Input %2 - %3.

Value that exceeded the input range was
input.
Blank that exceeded the input range was
input.
<Configuration Screen Errors>

No
Error Message
Unable to read Rack Information file

(HL8900Racks.xml). Displays default value.
The rack name %1 was not found.

Choose another rack.
Unable to read Column Information file
(HL8900Columns.xml).
Displays default value.
Unable to write Rack Information file

(HL8900Racks.xml).
User can cancel the operation.
5
6
The Rack Name (%1) is already registered.

Rack Name field is blank.
1 - 65
Read error of HL8900Racks.xml.

Occurs when relevant file cannot be
accessed in following folders.
<Enterprise Path>_OEMAmin, <EZChrom
Path>, <EZChrom Path>Japanese
The rack name received from EZChrom does
not exist in the list.
Read error of HL8900Columns.xml
Write error of Rack Information File.
Occurs when relevant file cannot be updated
in following folders.
Path>RacksWrite, C:Documents and
SettingsAll UsersApplication
DataChromatographySystemRecovery
Data
Existing rack name specified again.
Rack Name isn't specified.
1.9
Error Message List
(contd)
No
Error Message
Unable to write Columns Information file.

(HL8900Columns.xml)
User can cancel the operation.
8
9
Unable to register more than 256 columns.

Delete an unnecessary columns.
The date format is not correct. Format is ***.
10
11
12
Model Name field is blank.

ID Number field is blank.
The ID Number (%1) is already registered.
Write error of Column Information File.

Occurs when relevant file cannot be updated
in following folders.
Path>ColumnWrite, C:Documents and
SettingsAll UsersApplication
DataChromatographySystemRecovery
Data
Registration of 257th column was attempted.
Start-of-use date format is different from date
format of System.
Have not specified Model Name.
Have not specified the ID No.
Specified ID Number already exists.
<Setup Screen Errors>

No
Error Message
Unable to read Column Information file.

(HL8900Columns.xml)
Cannot change a column.
The value of (%1) must be equal to or less

than (%3) value, based on the Sampling
Period.
The value of (%1) must be equal to or

greater than (%3) value, based on the
Sampling Period.
The syringe speed must not be greater than

(%1), based on the Syringe Volume.
The injection port wash speed must not be
greater than (%1), based on the Syringe
Volume.
Make a maximum value greater than a
minimum value.
Input 0.0 into the "time" setup.
6
7
8
9
10
The same time is already registered.

Change the setting.
Input of the mixture ratio is required.
Make the sum total of the mixture ratio of
each pump 100%.
1 - 66
Read Error of HL8900Columns.xml.

Value outside of range (maximum) was
specified in setup of acquisition period and
sampling period.
%1: Ch1 Acquisition period/Ch2 Sampling
period
The outside of the range (minimum) was
specified in setup of acquiring period and
sampling period.
%1: Ch1 Acquiring period/Ch2 Sampling
period
The syringe speed exceeds the allowable
setting range.
The injection port wash speed exceeds the
allowable setting range.
The pressure maximum/minimum of the
pump is Maximum Minimum
Value other than 0.0 was input at "time" of the
1st line.
The same time as already input was input
again.
Either %B1 - %B6 or %R1 - %R3 was blank.
It is not an error if all are blank.
The sum total of the mixture ratio is not
100%.
1.9
(contd)
No
Error Message
11
12
13
The value for %1 must not be empty.

There must not be more than 92 rows.
The selection area contains first row. The
value for first row cannot be Delete/Cut.
Invalid values in first row.
Reset to default values.
Cannot Copy/Paste Time values.
Invalid Paste operation
Gradient Program specifies B3 and B5 in the
same step.
Gradient Program specifies B2 and B6 in the
same step.
The reagent consumption cannot be
calculated. Two or more lines are required in
the timetable.
Input an integer of one or more (maximum:
999) for the injection times.
14
15
16
17
18
19
20
21
22
23
A timetable of two or more lines is required

for automatic generation of RG process.
RG start time does not exist in a timetable.
"RG" process is not searched for. In order to
generate RG process automatically, two
numerical values of one or higher are
required for %B5, %B4 or %B3. They usually
exist above "%B5 = 100%" (early time).
Correct the timetable or perform automatic
generation of RG process by time
specification.
The value for %1 is empty.

The number of rows exceeds 92.
It was attempted to Delete/Cut the 1st row.
It was attempted to paste invalid cell
information to first row.
It was attempted to copy the Time.
Pasting was made between different data.
B3 and B5 were specified at the same step.
B2 and B6 were specified at the same step.
The first line of the timetable is empty.
[Calculation] button was pushed in a state

where a value other than an integer of 1 to
999 was input for number of injections on the
reagent consumption calculation screen.
Timetable contains only one line of values.
The time set as RG start time does not exist
in the actual timetable.
A step that agrees with the automatic
generation rule of RG does not exist.
<Setup Screen Errors> [WARNING]

No
1
Error Message
[Pump 2 flow rate 0 mL/min] is set in the time

program. Continue or not ?
(After setting flow rate, liquid delivery at R3
100% is recommended.)
"0" was set as pump2 flow rate in the time

program.
OK: Processing is continued. 0 check not
performed for 2nd and subsequent pump2
flow rates.
Cancel: Processing is stopped. The focus
is moved to an applicable cell.
1 - 67
1.9
Error Message List
<Status Screen Errors>

No
1
2
3
Error Message
Make a maximum value greater than a

minimum value.
Make the sum total of the mixture ratio of
each pump 100%.
Unable to read Column Information file
(HL8900Columns.xml).
Create in the column maintenance screen.
Pressure maximum/minimum of the pump is

Maximum Minimum
The sum total of the mixture ratio is not
100%.
Read error of HL8900Columns.xml (when
column information button is clicked)
Appears when relevant file cannot be
accessed in following holders.
<Enterprise Path>_OEMAmin,
<EZChrom Path>, <EZChrom
Path>Japanese
<System Errors>
No
Error Message
Unconnectable with the unit.
Unconnected with the unit. Please connect.
Connected with the unit, now. Unable to

close.
% 1 is the %2 mode.
Change into Constant Flow mode.
N2 gas valve is the %1 mode.

Change into ANALYSIS mode.
%1 purge valve is open.

Close the valve.
% 1 is under operation.
Disconnecting process cannot be performed.
N2 gas is under bubbling.

Disconnecting process cannot be performed.
No sufficient HD availability in the folder.
100MB or more of availability is required.
10
The number of X vial (Nx) of the rack

parameter is error.
Shut down the instrument icon of EZChrom
and check the setup of rack parameter.
1 - 68
The unit was undetectable by connection

processing.
A process accompanying transfer to the unit
such as single run, sequence run, baseline
check, etc. was requested in the
unconnected state.
Shutdown of instrument was attempted
during connection.
Sequence run etc. was attempted while
Pump1/Pump2 was operating at
[Purge/Constant Press/C1 Flow].
%1: Pump1/Pump2
%2: Purge/Constant Press/C1 Flow
Sequence run etc. was attempted while N2
gas valve was set at [BUBBLING/OPEN].
%1: BUBBLING/OPEN
Sequence run etc. was attempted while
purge valve of Pump1/Pump2 was set at
OPEN.
%1: Pump1/Pump2
The connection release button was pushed
during pump operation.
%1: Pump1/Pump2/Pump1, Pump2
The connection release button was pushed
during N2 gas bubbling.
With less than about 100MB of HD free
space, a preview run, single run, or sequence
run was attempted.
The setting of the rack parameter differs from
the actual value.
1.9
(contd)
No
Error Message
11
The number of Y vial (Ny) of the rack

parameter is error.
The number of X1 (mm) of the rack
parameter is error.
The number of Y1 (mm) of the rack
parameter is error.
The number of X2 (mm) of the rack
parameter is error.
The number of Y2 (mm) of the rack
parameter is error.
The number of Z (mm) of the rack parameter
is error.
The pattern of the rack parameter is error.
The cooling unit is set in the Hitachi L-8900
setup screen.
and release the setup of cooling unit in the
setting screen of L-8900.
The cooling unit is not set in the Hitachi
L-8900 setup screen.
and set up the cooling unit in the setting
screen of L-8900.
The pump purge valve is set in the Hitachi
and release the setup of pump purge valve in
the setting screen of L-8900.
The pump purge valve is not set in the
Hitachi L-8900 setup screen.
and set up the pump purge valve in the
The liquid volume sensor is set in the Hitachi
and release the setup of liquid volume sensor
in the setting screen of L-8900.

the actual value.
12
13
14
15
16
17
18
19
20
21
22
1 - 69

the actual value.

the actual value.

the actual value.

the actual value.

the actual value.

the actual value.
For the cooling unit:
the configuration information differs from the
unit information.
For the cooling unit:

unit information.
For the pump purge valve:

unit information.
For the pump purge valve:

unit information.
For the liquid volume sensor:

unit information.
1.9
Error Message List
(contd)
No
23
Error Message
25
26
The liquid volume sensor is not set in the

Hitachi L-8900 setup screen.
and set up the liquid volume sensor in the
Since this method does not have a data
sampling, a preview run cannot be carried
out.
The invalid vial number.
The pump has stopped.
27
28
The lamp of the detector is off.

The column oven has stopped.
29
Reactor has stopped
24
1 - 70
For the liquid volume sensor:

unit information.
Preview run of a method without data

acquisition (standby method or RG method
without data sampling) was attempted.
A vial No. set for Inj is invalid.
The pump had stopped at the time of run
start.
The lamp was off at the time of run start.
The column oven had stopped at the time of
run start.
The reactor had stopped at the time of run
start.
1.10
1.10
Status Transition
When each state is completed normally, the state changes as
shown below. (The suspended state is also included).
Current State
State Transition
Baseline Check
Preview Run
Single Run
Sequence Run
Idle
Idle
Idle
Washing Process
When an error occurs during operation (any of the runs), transition

occurs automatically to the state shown below, according to the
contents of error or the present state of instrument.
Module
Name
IFM
Pump1
Process and Transition Destination at

Error Occurrence
Error Content Error Analysis (including preview run,

Level
baseline test, warming-up
process)
IFM overall
Pressure
Limiter
Motor Initialize
Leak sensor
Pump2
Pressure Limit
Motor Initialize
Leak sensor
Detector
Auto Zero
Lamp
Leak sensor
Autosampler Injection valve
Syringe
Syringe valve
Arm X
Arm Y
Needle Z
Empty Cup
Detection
Leak sensor
Column
Temperature
Oven
Sensor
Control
Reactor
Temperature
Leak sensor
Cooling
Temperature
Rack
USB
Washing
Shutdown
0
0
Shutdown
Shutdown
Shutdown Display only

Shutdown
-
0
0
2
2
0
2
2
0
1
1
1
1
1
1
2
Shutdown
Shutdown
Washing Process
Washing Process
Shutdown
Washing Process
Washing Process
Shutdown
Washing Process (without P-Wash)
Washing Process
Shutdown
Shutdown
Shutdown
Shutdown
Shutdown
Shutdown
Shutdown
-
0
0
0
0
2
0
2
Shutdown
Shutdown
Shutdown
Shutdown
Washing Process
Shutdown
Washing Process
Shutdown
Shutdown
Shutdown
Shutdown
Shutdown
Shutdown
Display only
Display only
Display only
Display only
Display only
Shutdown Display only
1 - 71
1.11
Warming-up Process
Error Level
0: Shutdown
1: Washing Process without Plunger-Washing
2: Washing process after washing the plunger
NOTE: When an error that doesnt allow shift to the washing

process occurs, perform washing of the flow path
system by manual operation.
1.11
Warming-up Process
After turning on power to the main unit of L-8900, allow a
warming-up to stabilize the instrument fully before starting analysis
(stabilization of pump flow rate, temperature of column oven and
reactor, etc.).
The warming-up of instrument is automated by execution of the
following procedure.
<Steps of Warming-up Process>
(1)
Turn ON Pump1and Pump2.
(2)
Turn ON the Reactor.
(3)
The following Warming-up Process Time Program (standby

method) is performed.
1 - 72
1.11
Warming-up Process: PH 4.660-2622 ;Standby method

Pressure Limiter
Minimum (MPa)
Pump1
Pump2
0.0
0.0
Pump1
%B6 Flow Rate
(mL/min)
Time
(min)
%B1
0.0
100
1.0
100
1.1
2.0
2.1
3.0
3.1
4.0
4.1
5.0
5.1
100
7.5
100
9.0
10.5
12.0
13.5
15.0
100
20.0
(*2)
100
%B2
%B3
%B4
%B5
0.10
Column
Temperature
(C)
%R1
Maximum (MPa)
%R2
%R3
Pump2
Flow Rate
(mL/min)
100
0.10
57
(*1)
0.10
0.10
0.25
0.20
0.40
(*1)
*1: Set up the parameters of the 1st line of an analysis method.

*2: Input one of the following generally.
20 min.:
When RG start will be performed after the end of the warming-up process.
40 min.:
Other than the above.
1 - 73
100
50
50
0.35
(*1)
1.11
Warming-up Process
The standby method can be changed according to the purpose or

use.
NOTE: Be sure to perform the standby method by a single
run.
Pumps1&2 and the reactor start automatically only
when executing a single run.
NOTE: Execution of mild-start
The temperature of the column oven and the reactor
is not yet suitable for analysis immediately after
starting the L-8900.
If pump1 or pump2 is operated in this state, there is
a possibility that the main column and the reaction
column may be damaged.
Be sure to perform the warming-up process (mildstart) after starting the instrument.
1 - 74
1.12
1.12
RG Process
The RG process is a combination of the regeneration and
equilibration processes, which are included in the analysis
methods time program table.
By executing the RG process before analysis, we can get high
quality data even from the first round of measurement.
The RG process can be automatically generated based on a time
program table currently under editing (refer to 1.5.11).
The automatically generated RG process is changeable
depending on its purpose and usage.
The created RG process can be added as an analysis method to
the whole program sequence.
RG Process
UNK Sample
STD Sample
1 - 75
1.13
1.13
Washing Process
Washing Process
After analysis is completed, it shifts to the washing process
automatically. The flow path is flushed out, and then it stops.
Also if an error occurs and an abnormality appears in the
instrument, shift is made to the washing process automatically.
<Steps of Washing Process>
(1)
The heater of reactor is turned OFF.
(2)
Pump washing is performed.
(3)
The following Washing Process Time Program is performed.
Washing Process
Pressure Limiter
Mininum (MPa)
Maximum (MPa)
Pump1
Pump2
0.0
0.0
20.0
5.0
Time
(min)
%B1
%B2
%B3
%B4
%B5
0.0
100
0.1
100
%B6
Pump1
Flow Rate
(mL/min)
0.20
Column
Temperature
(C)
%R1
50
%R2
%R3
Pump2
Flow Rate
(mL/min)
100
0.18
70
15.0
100
100
15.1
100
100
100
100
100
20.0
20.1
59.0
60.0
50
100
(4)
Pump1 and 2 are turned OFF.
(5)
The lamp of detector is turned OFF.
Since the above-mentioned program is stored in the instrument,

the process cannot be changed.
1 - 76
1.14
NOTE: After analysis is completed, shift is made to the

washing process automatically. The flow path is
flushed out, and then it stops.
When shutting off the power supply of instrument
without waiting for this process, flush out the flow
path system by one of the following methods.
1. Remove the sample vials from the autosampler.
Execute a single run with the analysis program
currently used.
(The washing process is executed and
automatically stops 1 hour later.)
2. Stop pump2 by manual operation.
Set the reactor into OFF state.
Deliver solvent for 30 min. at a flow rate of
0.2 mL/min with pumps1 and 2 (B1: 100 %/
R3: 100%).
And then stop the pumps.
1.14
Automatic-Stop Processing by Module Operation

If the instrument is left in the state of module operation for a long
time, automatic-stop processing will be performed in order to
prevent waste of reagent.
Uncontrolled Time (h)
Action
Pump2 is stopped
Reactor Heater is stopped
Pump1 is stopped
4.0
4.5
When module operation is re-performed during the count of the

uncontrolled time: The operation is performed and the
uncontrolled time is reset (count is re-started at 0 minute).
Automatic-stop process is performed only for the uncontrolled
state in the module operation. After the end of the sequence, it
shifts to the washing process without being dependent on the
uncontrolled time during the sequence run.
1 - 77
1.15
1.15
Status Display of Autosampler Tray
Status Display of Autosampler Tray

Select the Control/Show Autosampler Tray in the main menu.
The state of the autosampler rack currently used with L-8900 can
be shown by a graphic illustration.
A color display shows the state of each vial.

Color
Analysis State
Black
Green
Red
Under Analysis
Completed Analysis
Failed Analysis
1 - 78
1.16
1.16
Ingredient Weight Calculation Function

EZChrom Elite for HITACHI Amino Acid Analyzer has a function of
calculating ingredient weight, which quantifies each ingredient on
the basis of its concentration. The ingredient weight can be
worked out by the below formula.
Ingredient Weight (ng) = MOLConcentration (nmol) Molecular Weight
In Molecular Weight table, the ingredient names and molecular

weights should be set in advance. Ingredients can be added to
and modified in the table.
The Molecular Weight table (M_Weight.csv) file is a read-only type
for the purpose of data protection. Addition or change should
thus be made after releasing the RO check box of the Properties
attribute.
The ingredient name entered in analysis method should be
identical to that in Molecular Weight table. Otherwise, its
molecular weight cannot be obtained. (It is case sensitive.)
<File Location> EZChrom Folder Hitachi AAA Calc
<File Name>
M_Weight.csv
How to set Method:
Select Menu bar/Method/Method option to open the Custom
Parameters window, and set the following parameters.
Parameter name : CalcMol
Type
: Per-peak
Return value
: Number
Data source file : EZChrom folder HitachiAAACalc
HL8900CalcMol_ESTD.dll
1 - 79
1.16
Ingredient Weight Calculation Function
If an error occurs in ingredient weight calculation, the following

numerical values are output to the display column of the ingredient
weight calculation result.
This means that ingredient weight calculation could not be
performed correctly.
Calculation
Result Display
State
-9999.0
Ingredient name identical to that in Molecular

Weight table file does not exist.
Unable to locate Molecular Weight table file.
Molecular Weight value, in which row the
ingredient name is identical to that in
Molecular Weight table file, is set incorrectly.
-8888.0
-7777.0
1 - 80
2.
OPERATION
2.1
Operational Flow.................................................................................................... 2-1
2.2
Preoperational Procedure up to Power-On ............................................................ 2-2

2.2.1
Installation of Buffer Solutions and Ninhydrin Reagent
(when installing newly) ............................................................................. 2-2
2.2.2
Installation of Autosampler Washing Solution .......................................... 2-2
2.3
Startup of L-8900................................................................................................... 2-3

2.3.1
Power-On of Analyzer .............................................................................. 2-3
2.3.2
Adjustment and Check of N2 Gas Pressure.............................................. 2-3
2.3.3
Replacement of Buffer Solution and Ninhydrin Reagent .......................... 2-3
2.3.4
Substitution of Ninhydrin Reagent with N2 Gas ........................................ 2-4
2.3.5
Substitution of Liquid in Flow Path and Pump .......................................... 2-4
2.4
Startup of EZChrom Elite for HITACHI................................................................... 2-5
2.4.1
Startup of Windows XP .......................................................................... 2-5
2.4.2
Startup of EZChrom Elite for Hitachi ........................................................ 2-6
2.5
Instrument Configuration ....................................................................................... 2-7

2.5.1
Creation of New Instrument...................................................................... 2-7
2.5.2
Instrument Configuration.......................................................................... 2-9
2.6
Preoperational Procedure prior to Analysis ............................................................ 2-13

2.6.1
Startup of Instrument Icon........................................................................ 2-13
2.6.2
Setup of Pump1 Flow Rate (purging of buffer solution)............................ 2-14
2.6.3
Attachment of Column.............................................................................. 2-15
2.6.4
Setup of Pump2 Flow Rate (purging of reaction solution) ........................ 2-16
2.6.5
Setup of Autosampler (deaeration of syringe) .......................................... 2-16
2.6.6
Zeroing of Pump Pressure ....................................................................... 2-17
2.6.7
Setting of Standard Sample and Unknown Sample.................................. 2-18
2.6.8
Check Points before Start (routine check items) ...................................... 2-19
2.7
Creation of Method ................................................................................................ 2-20

2.7.1
Setup of Instrument Conditions ................................................................ 2-20
2.7.2
Calculation of Reagent Consumption ....................................................... 2-22
2.7.3
2.7.4
2.7.5
Saving of Method ..................................................................................... 2-23

Creation and Saving of RG Process Method............................................ 2-23
Editing and Saving of Warm-up Process.................................................. 2-25
2.8
Sequence Run (preliminary analysis)..................................................................... 2-27

2.8.1
Creation and Saving of Sequence Table.................................................. 2-27
2.8.2
Execution of Warm-up and Baseline Check ............................................. 2-33
2.8.3
Execution of Sequence Run..................................................................... 2-35
2.9
Waveform Processing and Peak Identification....................................................... 2-37

2.9.1
Waveform Processing while Viewing Chromatogram ............................... 2-37
2.9.2
Peak Identification.................................................................................... 2-43
2.10 Generation of Report ............................................................................................. 2-50

2.10.1 Report Setup............................................................................................ 2-50
2.11 Sequence Run (execution of continuous analysis)................................................. 2-52
2.11.1 Creation and Saving of Sequence Table.................................................. 2-52
2.11.2 Execution of Warm-up ............................................................................. 2-58
2.11.3 Execution of Sequence Run (addition of Run Queue) .............................. 2-59
2.12 Sequence Processing............................................................................................ 2-61
2.12.1 Check of Working Curve .......................................................................... 2-61
2.12.2 Sequence Processing .............................................................................. 2-61
2.12.3 Creation of Sequence Table for Reprocessing......................................... 2-62
2.12.4 Execution of Sequence Run..................................................................... 2-66
2.13 Termination of EZChrom Elite for HITACHI ........................................................... 2-68
2.13.1 Termination of EZChrom Elite .................................................................. 2-68
2.13.2 Termination of Windows XP ................................................................... 2-69

2.13.3 Forced Termination of Program at Occurrence of Trouble ....................... 2-70
2.14 Shutdown of L-8900............................................................................................... 2-70
2.15 Precautions on Measurements .............................................................................. 2-71
2.1
2.
Operational Flow
This section describes by way of an operational flow the procedure

for creating a working curve with a standard sample and
measuring the concentration of an unknown sample.
As an example, the protein hydrolyzate method (the standard
analyzing method) is used.
Operational Procedure
(Item No.)
Preoperational Procedure up to Power-On
Startup of L-8900
Already
set
Startup of EZChrom Elite
Instrument Configuration
Preoperational Procedure prior to Analysis

Analytical method already created
2.1
OPERATION
Creation of Method
Sequence Run
2.2
Check of N2 gas pressure and setting of buffer

solutions and ninhydrin reagent
Power on of L-8900
2.3
2.4
Power-on of PC and startup of Windows XP

and EZChrom Elite for HITACHI
2.5
Setup of instrument configuration (with/without

sample cooler, rack parameters, column used,
etc.)
2.6
Check of remaining volume of buffer

solution/ninhydrin reagent, setting of standard
sample and unknown sample, etc.
2.7
2.8
Setup of analytical conditions (pump flow rate,

column temperature, etc.) for each module
Execution of single run with preliminary sample
for continuous analysis (review of analytical
program)
Waveform processing and peak identification
from data obtained through sequence run.
Waveform Processing and

Peak Identification
2.9
Setup of Report
Setup of layout for automatic report printing at

2.10 continuous analysis (processing)
Sequence Run
2.11
Continuous analysis
(standard sample/unknown sample)
Sequence Processing/Report Print
Reprocessing by use of existing data (creation

2.12 of working curve/report printing)
Termination of EZChrom Elite
Termination of Windows XP and EZChrom

2.13 Elite for HITACHI and power-off of PC
Shutdown of L-8900
Power-off of L-8900
2.14
2-1
2.2
2.2
2.2.1 Installation of
Buffer Solutions
and Ninhydrin
Reagent (when
installing newly)
The procedure in the case of newly installing buffer solutions and

ninhydrin reagent is indicated here.
Refer to section 2.3.3 for exchanging buffer solution or ninhydrin
reagent that is already installed.
(1)
Set the bottles filled with buffer solutions B1 to B6 at their

positions. Secure the bottle caps (but don't overtighten
them).
(2)
Connect the R1 tube to the ninhydrin reagent bottle and

secure the bottle cap.
(3)
Connect the R2 tube to the ninhydrin buffer solution bottle

and secure the bottle cap.
(4)
Fill the R3 bottle with about 1 L of [5% ethanol], connect the

R3 tube and secure the bottle cap.
(5)
Attach a protective cover to each of the R1 and R2 bottles.
(6)
Set an empty waste solution tank (10 L) in place.
NOTICE: 1. Before setting or replacing the buffer

solution/ninhydrin reagent bottle, be sure to
set the N2 gas pressure to 0 kPa (set the N2
gas selector switch to OPEN and wait for
3 minutes).
2. Handle the R1 and R2 bottles with care.
Also be sure to attach a protective cover to
each of them for safety.
2.2.2 Installation of
Autosampler
Washing
Solution
Fill the furnished rinsing solution bottle (C-1, 1 L) with distilled

water, set it at the specified position and secure its cap.
NOTICE: Check the liquid level often, and replenish it
when necessary (about 1 mL/sample is
consumed).
2-2
2.3.1
2.3
Startup of L-8900
2.3.1 Power-On of
Analyzer
2.3.2 Adjustment and

Check of N2 Gas
Pressure
(1)
Turn on the POWER switch on the right side of the analyzer.

A self-check of each unit is carried out.
(2)
The self-check result for each unit is displayed after starting

up EZChrom Elite and pressing the Connect button in the
Instrument Status (L-8900 tab) screen (only if an error
occurs).
(1)
Open the main valve of the N2 gas cylinder and adjust the
reading on the pressure reducing valve to 50 to 100 kPa (0.5
to 1.0 kgf/cm2).
(2)
Turn clockwise gently the N2 gas regulator on the front of the

analyzer so that the pressure gauge reads 34 to 40 kPa (0.35
to 0.40 kgf/cm2).
(3)
When the N2 gas regulator on the front of the analyzer is

already adjusted, check the pressure gauge reading and
make fine adjustment so that the pressure gauge reads
34 to 40 kPa (0.35 to 0.40 kgf/cm2).
NOTICE: 1. Absolutely avoid increasing the N2 gas

supply pressure to the analyzer above
100 kPa (1.0 kgf/cm2).
2. Absolutely avoid increasing the pressure in
the analyzer above 50 kPa (0.5 kgf/cm2).
3. The pressure rises slowly. So allow 1 to
2 minutes for adjustment.
2.3.3 Replacement of
Buffer Solution
and Ninhydrin
Reagent
Given below is the procedure for replacement of the buffer solution

and ninhydrin reagent. For newly setting them, refer to 2.2.1.
(1)
Make sure that pumps1 and 2 are OFF, turn to OPEN the
selector button on the N2 gas unit panel at the front of the
analyzer and wait until the pressure drops to 0 kPa (about
3 minutes).
(2)
Open the cap of the buffer solution or ninhydrin reagent bottle

to be replaced, make the replacement and then secure the
cap again.
2-3
2.3
Startup of L-8900
(3)
Set to ANAL the selector button of the N2 gas unit and make
sure that the pressure gauge reads 34 to 40 kPa (0.35 to
2
0.40 kgf/cm ).
NOTICE: 1. Before setting or replacing buffer

solution/ninhydrin reagent bottle, be sure to
set the N2 gas pressure to 0 kPa (set the
N2 gas selector button to OPEN and wait for
3 minutes).
2. Handle the R1 and R2 bottles with care.
Also be sure to attach a protective cover to
each of them for safety.
2.3.4 Substitution of
Ninhydrin
Reagent with N2
Gas
This procedure is required for newly setting the ninhydrin reagent.

Start the EZChrom Elite for Hitachi. And click the Bubbling
button in the Instrument Status (Option tab) screen (bubbling time:
30 min).
N2 gas is introduced through the bubbling tube of the R1 and R2
reagent bottles and is discharged, whereby air is automatically
eliminated from the bottles. It ends automatically after 30 minutes.
NOTICE: Carry out this operation every time the ninhydrin
reagent is replaced.
2.3.5 Substitution of
Liquid in Flow
Path and Pump
(1)
Start the EZChrom Elite for Hitachi. And click the Purge
button of pump1 (pump2, when reaction liquid is exchanged)
in the Instrument Status (Option tab) screen.
(2)
Set the mixture ratio of the replaced solution to 100%.
(3)
Open the drain valve. Click the Start button and turn ON
the pump. (In the case of the full-specifications version, the
purge valve opens automatically.)
(4)
Feed the solution for about 2 minutes.

again and the purge is ended.
(5)
Close the drain valve. (In the case of the full-specifications

version, the purge valve closes automatically.)
2-4
Click the Start button
2.4.1
2.4
Startup of EZChrom Elite for HITACHI
2.4.1 Startup of
Windows XP
(1)
Upon powering on the PC, Windows XP starts automatically.
(2)
According to the following message, press the Ctrl, Alt and

Delete keys simultaneously.
Press Ctrl + Alt + Delete to logon.
(3)
The logon dialog box appears.

Password.
Example: User name
Password
Enter a User name and
HITACHI
HITACHI
User name
HITACHI
Password
OK
Cancel
Click the OK button to log on the system.
2-5
Options
2.4
Startup of EZChrom Elite for HITACHI
2.4.2 Startup of
EZChrom Elite
for Hitachi
(1)
Click Start/All Programs/Chromatography/EZChrom Elite

on the Administrator screen.
(2)
The EZChrom Elite for HITACHI program starts.
2-6
2.5.1
2.5
Create a new instrument and make setting for each module
included in the system configuration in the procedure given here.
When the instrument configuration is already set, skip to 2.6.
2.5.1 Creation of New

Instrument
(1)
Start up EZChrom Elite for HITACHI, click the right button of

the mouse in the main screen and select New/Instrument.
(2)
When an Instrument icon is created, change the instrument

name.
2-7
2.5
(3)
Click in the Instrument icon, and select Rename.
(4)
Enter L-8900 for the instrument name.
2-8
2.5.2
2.5.2 Instrument
Configuration
(1)
Click the right button of the mouse on the Instrument icon

and select the Configure button. The Instrument
Configuration setup dialog box will open.
(2)
Select Hitachi L-8900 Series AAA in the Instrument type

field. The following message will appear. Click the OK
button.
(3)
Click the Configure button.

will open.
2-9
L-8900 Series AAA dialog box
2.5
(4)
Set the instrument configuration according to the system

configuration.
Select L-8900 in this dialog box.
To select a module to be used, click the module icon followed
by the right arrow button (green) or double-click the module
icon. The selected module enters the Configured modules
list. For a module selected by mistake, click the module icon
followed by the left arrow button (red).
As shown above, the ? mark is attached to the L-8900

module icon. This indicates that the instrument
configuration is not finished.
(5)
Click the Auto Configuration button. The contents of

configuration are acquired automatically.
(6)
Click the right button of the mouse on the L-8900 module

icon and select Open.
2 - 10
2.5.2
(a) Click the Options button.
(b) The Configuration Options dialog box will appear.

Turn on the respective check boxes of System Suitability
and Baseline Check (by putting a check mark there) and
then click the OK button.
2 - 11
2.5
System Suitability
Selected for use of the system suitability test option.
Selection of this option enables calculation of the
theoretical plate number (which indicates the column
performance), degree of separation, symmetry
coefficient and other parameters.
Baseline Check
Selected for use of the baseline check option.
Selection of this option enables check of the baseline
noise and drift. The option is useful for check of the
system stability.
(7)
After conducting the above operations, click the OK button.
(8)
The Instrument Configuration dialog box will reappear.

The instrument configuration has now been finished.
2 - 12
2.6.1
2.6
2.6.1 Startup of
Instrument Icon
(1)
For opening the measurement screen, select the instrument

icon L-8900 to be used for measurement, double-click or
click the right button of the mouse and select Open.
(2)
The instrument icon (measurement condition setup and

measurement screen) will start.
2 - 13
2.6
2.6.2 Setup of Pump1

Flow Rate
(purging of
buffer solution)
(1)
Select Control/Instrument Status from the main menu to

open the Instrument Status (L-8900 tab) screen.
(2)
Click the Connect button. On-line connection takes about 2

minutes. Make sure that Idle is indicated in the System
Status field.
(3)
Click the Pump1 Purge button in the Instrument Status

(option tab) screen.
2 - 14
2.6.3
(4)
Select Washing (C1).
Click the Start button.
(5)
Open the drain valve of pump1.
(6)
Click the Pump1 SW button in the Manual ON/OFF area and

operate the pump for about 2 minutes.
(7)
Change to auxiliary liquid/regenerating solution. Click the

Start button after setting the mixture ratio of B5 and B6 to
50% each.
(8)
The pump is operated for about 2 minutes.

Start button again and the purge is ended.
(9)
Change to buffer solution. Click the Start button after

setting the mixture ratio of B1 to B4 to 25% each.
Then click the
(10) After operating the pump for about 8 minutes, click the Start
button again. The purge is completed.
(11) Close the drain valve of pump1.
2.6.3 Attachment of
Column
(1)
in the Manual Operation

Click the Pump1 button
area. Set flow rate of 0.200 mL/min. Change the Mixing
Ratio of buffer solution B1 to 100% and click the OK button.
(2)
Click the Pump1 SW button in the Manual ON/OFF area to

operate pump1.
(3)
Make sure that the buffer solution drips from the end of tube
to be connected to the ammonia filter column inlet.
Then, connect an ammonia filter column to the inlet and
outlet tubes.
(4)
In the same way, make sure that the buffer solution drips
from the end of tube to be connected to the main column inlet,
then stop pump1 and connect a main column to the inlet and
outlet tubes.
(5)
Place the main column in the column oven.
2 - 15
2.6
(1)
2.6.4 Setup of Pump2
Flow Rate
(purging of
reaction solution)
2.6.5 Setup of
Autosampler
(deaeration of
syringe)
Click the Pump2 button

area.
in the Manual Operation
(2)
Change the Mixing Ratio of solvents R1 to R3 to 33, 33 and

34% and click the Start button.
(3)
Open the drain valve of pump2.
(4)
After operating the pump for about 6 minutes, click the Start
button again. The purge is completed.
(5)
Close the drain valve of pump2.
(1)
Click the Autosampler button

Operation area.
2 - 16
in the Manual
2.6.6
2.6.6 Zeroing of
Pump Pressure
(2)
Select Sampler Wash and click the OK button.

The needle and autosampler flow path are now washed.
For deaeration of the syringe, carry out Sampler Wash three
times. When using the instrument for the first time, repeat
Sampler Wash at least ten times and make sure that there is
no air bubble left in the syringe.
(3)
Click the Autosampler button

in the Manual
Operation area again.
Select Pump Wash and click the OK button.
The plungers of pumps1 and 2 are washed.
(1)
Make sure that pumps1 and 2 are stopped.
(2)
Open the drain valves of pumps1 and 2.
(3)
Click the Pump Pressure 0 Adjust button

Maintenance area.
(4)
Select Pump1 and click the OK button. The pressure of

pump1 is adjusted to 0. Make sure that 0 is indicated for
Pump1 - Pressure in the Instrument Status screen.
(5)
again in
Click the Pump Pressure 0 Adjust button
the Maintenance area. After that, select Pump2 and click
the OK button. The pressure of pump2 is adjusted to 0.
Make sure that 0 is indicated for Pump2 - Pressure in the
Instrument Status screen.
(6)
Close the drain valves of pumps1 and 2
2 - 17
in the
2.6
2.6.7 Setting of
(1)
Standard Sample
and Unknown
Sample
(2)
Fill the sample vials with standard sample and unknown

sample. And tighten the septums and caps.
Arrange the vials containing standard sample and unknown
sample into the sample rack and set it in the autosampler
NOTICE: The sample vial holds about 2000 L.

However, avoid pouring in sample in excess of
1500 L. Otherwise the sample injection
accuracy (reproducibility) may be adversely
affected.
2 - 18
2.6.8
2.6.8 Check Points

before Start
(routine check
items)
Before starting analysis, be sure to check the following items.

Unit
No.
Check Point
N2 gas system
N2 gas pressure indication normal?2

(34 to 40 kPa (0.35 to 0.40 kgf/cm )
N2 gas selector switch at ANAL
position?
Volume of buffer solution (B1 to B6)
sufficient?
Volume of ninhydrin reagent (R1 and
R2) sufficient?
Volume of 5%EtOH (R3) and distilled
water (C1) sufficient?
Waste solution tank empty?
Liquid volume in deaerator tube
sufficient?
No liquid leakage/bleeding on pump
unit, sampler unit or flow path
system?
Flow rate and pressure of pump1/2
normal?
Upper and lower pressure limits of
pump1/2 proper?
Sample vial position (number)
correct?
Specified septum mounted properly
on sample vial?
Sample volume sufficient?
Sample vial mounted securely?
Any air bubbles in syringe?
Temperature indication normal?
No liquid leakage/bleeding on any
column joint?
Temperature indication normal?
No liquid leakage/bleeding on any
reaction column joint or inlet/outlet
pipe? (For the sake of safety, check
this item before starting operation or
after cooling down the instrument.)
Instrument configuration selected
properly?
Contents of RG process file and
analytical file (method) proper?
Contents of sequence table proper?
Data processing parameters proper?
Printer setting proper?
Printing paper sufficient?
Baseline noise value normal?
2
Flow path
system
1
2
3
4
5
6
Pump
1
2
Autosampler
1
2
Column oven
3
4
5
1
2
Reaction unit
1
2
Data
processor
1
2
3
4
5
6
7
2 - 19
Check
Mark
2.7
2.7
Creation of Method
Creation of Method
2.7.1 Setup of
Instrument
Conditions
When an analytical method is already created, skip to 2.11

(execution of continuous analysis). Wavelength processing and
peak identification are possible in sequence processing after data
acquisition as well.
(1)
For creation of a new method, open the Instrument icon from

EZChrom Elite and select Create or modify a method on
the Instrument Wizard screen.
The Instrument Setup screen will start.
(2)
When a method is already created, click Open/Open Method

on the tool bar and select a file.
The Instrument Setup screen will start.
(3)
Click the L-8900 tab.

Make setting for each module.
2 - 20
2.7.1
(4)
Click the Aux Traces tab.

Set each item.
(5)
Click the Baseline Check tab.

Set each item.
NOTE: If the setup of baseline check is turned ON, it will

be checked automatically before the start of
analysis. Moreover, if the threshold is exceeded
and the result is NG, operation will be decided by
the setting of processing at the time of NG in the
sequence table.
2 - 21
2.7
Creation of Method
(6)
Click the Trigger tab.

Set each item.
Set External for Type. This setting starts data acquisition

after completion of injection with the autosampler.
2.7.2 Calculation of
Reagent
Consumption
(1)
After setup of the instrument conditions, click the Calculate

to open the
Reagent Consumption icon button
Calculate Reagent Consumption dialog box.
(2)
Enter the number of sample injections. Then, click the

Calculation button to find the reagent consumption.
NOTICE: Make sure that the respective volumes of buffer

solutions (B1 to B6) and reaction solutions (R1
to R3) are sufficient. Also start analysis after
emptying the waste solution tank.
2 - 22
2.7.3
2.7.3 Saving of
Method
Save the settings (instrument conditions) so far. For method

saving, select File/Method/Save As. (For any other method,
make setting after data acquisition.)
Let us save the method under the name of L-8900 Test as an

example.
Enter the file name and click the Save button.
2.7.4 Creation and

Saving of RG
Process Method
(1)
After setup of the instrument conditions, click the RG

process Auto Generation button.
(2) Specify RG Data Sampling ON/OFF and click the OK button.

An RG process method is automatically generated on the
basis of the currently indicated method.
2 - 23
2.7
Creation of Method
NOTICE: Upon clicking OK in the Auto RG dialog box, the

currently indicated method is substituted with the
automatically generated RG process method.
So, be sure to save the method before clicking
the OK button.
(3)
(4)
Save the automatically generated RG process method.

For method saving, select File/Method/Save As.
Save the RG process method under the name of L-8900

Test RG as an example.
Enter the file name and click the Save button.
2 - 24
2.7.5
2.7.5 Editing and

Saving of Warmup Process
(1)
Click Open/Open Method in the menu bar, select a warm-up

method (PH 4.6 60-2622(Stand-by).met)and click Open.
(2)
Edit the warm-up method (PH 4.6 60-2622(Stand-by).met)
2 - 25
2.7
Creation of Method
(3)
Change the Flow rate/Temp of Pump1 and the Flow rate of

Pump2 at 15.0 min in the time program, to conditions created
at section 2.7.1.
(4)
Save the warm-up method (PH 4.6 60-2622(Stand-by).met)

in overwrite mode by clicking File/Method/Save.
2 - 26
2.8.1
2.8
Sequence Run (preliminary analysis)
2.8.1 Creation and

Saving of
Sequence Table
Measure the standard sample as a preliminary sample and carry

out waveform processing and peak identification before continuous
analysis. This procedure allows working curve creation and
automatic report printing in parallel with execution of continuous
analysis.
(1)
Click the Instrument Wizard icon

Instrument Wizard.
(2)
Click the Create a sequence button.
(3)
The Sequence Wizard will start.
to start up the
Make sure that the method to be used for measurement is

set in the Method field. L-8900 Test created in 2.7.1 is
specified this time.
In the Data File Type area, select For acquisition.
Other parameters should be set up the same as those on the
following screen.
2 - 27
2.8
After completion of setup, click the Next button.

(4)
Make setting for an unknown sample.
2 - 28
2.8.1
Make setting as follows.

Sample ID
Enter a sample ID. Select <Increment Number> and
<Method Name> by clicking the blue right arrow button.
Data path
Enter a path name for data saving or select an existing path
name by clicking the Open File button.
Data file
Select a Sample ID by clicking the blue right arrow button.
Number of unknown runs in sequence :
Repetitions per run
:
1
1

above screen.
(5)
Click the Next button.
(6)
Make setting for the autosampler.
2 - 29
2.8

Unknown vials of sequence First vial : 1
(Check the standard sample position in the sample rack and
enter a Vial No.)
Autosampler injection volume
(7)
20 (L)
In this dialog box, Calibration ID and Calibration File are

automatically set up the same as in the dialog box for the
unknown sample.
Set 0 for the number of calibration levels.
For other parameters, retain their initial settings.
(Make sure that they are set up the same as those on the
above screen.)
(8)
Click the Finish button.

automatically.
2 - 30
The sequence edit screen will open
2.8.1
(9)
Select the 1st line in the sequence table, click the right button
of the mouse and select Insert Line to add one line to the
sequence table.
(10) Add an RG process method.

Level
: 0
Vial
: 1
Volume (L) : 0 (Be sure to set the sample injection volume
to 0 L.)
Method
: L-8900_Test_RG.met
2 - 31
2.8
NOTICE: 1. Make sure that the vial number is correct.

If an invalid vial number (invalid numerical
value or blank) is set, the run is stopped as
soon as the invalid value is recognized and
subsequent samples are not analyzed.
Valid vial numbers
Standard rack: 1 to 200
(Rack No. + vial No. is input at the time of
multi-rack use. Example of input: A200)
2. Make sure that the autosampler injection
volume is proper. If an invalid injection
volume is set, the run is stopped as soon as
the invalid value is recognized and
(11) Click File/Sequence/Save As.

the name of L-8900 Test.
2 - 32
Save the sequence under
2.8.2
2.8.2 Execution of
Warm-up and
Baseline Check
Make sure that the Connect button is clicked on the Instrument

Status screen (make sure that Idle is indicated in the System
Status field).
(1)
Click the Single Run

Acquisition dialog box.
(2)
button to open the Single Run
Method
: PH 4.6 60-2622(Stand-by).met
Vial
: 1
Injection volume : 0 L
Upon clicking the Start button, Single Run is executed.
Open the Instrument Status screen and make sure that the
warm-up process is started.
System Status
Pump1
Pump2
Reactor Heater
(3)
: Running (warm-up)
: ON
: ON
: ON
Upon completion of the warm-up process, make sure that

Idle is indicated in the System Status field and open the
analytical method (L-8900 Test.met).
2 - 33
2.8
and check the baseline stability.
(4)
Execute Preview Run
(5)
When the baseline becomes stable, click Stop Run

terminate Preview Run.
(6)
Click Control/Baseline Check.
(7)
Set up as follows and click Submit button.

Baseline check time
: 1 min
Baseline check finish time
(when inadequate)
: 1 min
Noise calculation method : ASTM
Threshold value (Noise) : VIS1 150, and VIS2 600
(8)
A baseline check is started.
2 - 34
to
2.8.3
(9)
A judgment result is indicated at the end of baseline check.
The baseline is good if Passed is indicated for Overall

Status.
If Failed is indicated, carry out baseline check again.
2.8.3 Execution of
Sequence Run
(1)
Click Sequence Run
at the upper part of the screen.
Make sure that L-8900 Test saved earlier is set in the

Sequence name field.
2 - 35
2.8
(2)
When it is desired to automatically print out reports after

measurement, turn on the check box of Print method reports
(by putting a check mark there). Reports on all the samples
contained in the sequence table are automatically printed out
in the template specified in Method Custom Report.
(Make sure that other parameters are set up the same as
those on the above screen.)
(3)
After completion of setup, click the Start button.

With the above operations, Sequence Run is executed.
First, the RG process method is executed.
System Status: Running (RG)
Next, the analytical method is executed.
System Status: Running (Acquisition Data)
Check the measurement results (separation, etc.).
If adjustment of the time table is necessary, complete the
analytical method by modifying Sequence Table with
reference to the separating conditions in the instruction
manual (main unit ver.).
Upon completion of measurement, the system transits to the
wash process automatically.
System Status: Running (Wash)
Upon completion of the wash process, the System Status
returns to Idle.
For turning ON pump1, pump2 and reactor again, operate the
relevant buttons in the Manual Operation area of the
Instrument Status screen. The photometer lamp is turned
ON automatically by executing any of Preview Run, Single
Run and Sequence Run.
When the wash process is finished, you can safely power off
the L-8900. Before turning off power, click the Disconnect
button and make sure that Uninitialized is indicated in the
System Status field.
2 - 36
2.9.1
2.9
Waveform Processing and Peak Identification
2.9.1 Waveform
Processing while
Viewing
Chromatogram
Default integration parameters are used for simple

chromatography processing. However, special integration is
required in some cases. As an example, add an event of
integration off to the method in the procedure given below.
For details of setup of other integration parameters, refer to the
user guide furnished with EZChrom Elite.
(1)
Click File/Data/Open to display the data to be subjected to

waveform processing.
* The data obtained from Sequence Run explained in the
previous section remains displayed after completion of
measurement. The data screen need not be opened again
unless it has been closed.
Select data L-8900 Test.DAT and click the Open button.
2 - 37
2.9
Data before Waveform Processing
(2)
Select 1: VIS 1 on the toolbar to carry out wavelength

processing on channel 1 side.
(3)
Click the Analyze button

and display the baseline.
to integrate the chromatogram
Data before Integration Off
2 - 38
2.9.1
(4)
For adding the Integration Off event, click the right button of
the mouse on the chromatogram and select Graphical
programming/Integration Off. According to the guidance
in the status bar at the bottom of the window, click the mouse
at the start point of the area for integration off on the
chromatogram. (Select a part containing peaks.)
Then, click the mouse again at the desired restart point of
integration. The dialog box shown below will appear.
The clicked points are indicated as Start Time and Stop

Time. Processing is not made between these two points.
No numerical value is required for this event. So 0 is set
for Value. For adding the event to the method and
reprocessing the chromatogram, click the Analyze Now
button. If the Add to Table button is selected, the event is
added to the integration table, but processing is not carried
out.
When the button of Insert into Integration Events table is
selected, the event is added to the integration table of the
method and used for all the chromatograms analyzed by the
method.
When the button of Insert into Manual Integration Fixes
table is selected, the event is added to the manual
integration table for the current chromatogram and applied to
it alone.
2 - 39
2.9
Data after Integration Off
(5)
The integration event is input in the integration table.

For displaying this table, click the Integration Events button
shown below will appear.
The integration table
* Width and Threshold are default waveform processing

parameters. Waveform processing is impossible unless
these parameters are set.
2 - 40
2.9.1
Peak Width
Used to integrate data points before application of the integration
algorithm or to calculate a smoothing value. Integration yields
good results when the number of data points for one peak is 20.
In case of oversampling (sampling frequency too high), this
parameter is used to obtain an average value of data so that the
integration algorithm recognizes 20 data points in the peak.
For graphical setting of the [Width] value, make setting for the
narrowest peak in the chromatogram. Note, however, that a new
[Width] event should be input each time the peak width is doubled.
Threshold (Detection sensitivity)
First-order derivative. Used to discriminate the peak start and
end points from the baseline noise and drift in the integration
algorithm. For graphical setting of the [Threshold] value, select a
part of the baseline. The recommended value for [Threshold] is
determined according to the maximum value of 1st-order derivative
obtained from the selected part of baseline.
The following figure exemplifies the influence of the [Width] and

[Threshold] values of peak on the baseline.
Peak width
Peak width too small
Optimum value
Peak width too large
Detection
sensitivity
Detection sensitivity
value too small
Optimum value
Detection sensitivity
value too large
If both of [Width] and [Threshold] values are improper (too large or

too small), the peak may not be detected.
2 - 41
2.9
(6)
Select 2: VIS 2 on the toolbar to carry out waveform

processing on channel 2 side.
(7)
Repeat steps (3) to (5) to create an integration table on

channel 2 side the same as on channel 1 side.
2 - 42
2.9.2
2.9.2 Peak
Identification
(1)
Display the data to be subjected to peak identification and

click the Analyze
processing.
(2)
button to carry out waveform
Click the right button of the mouse and select Graphical

Programming/Define Single Peak.
2 - 43
2.9
The retention time of the initially detected peak is indicated.

For adding this peak to the peak table, make setting for the
peak. To avoid adding this peak to the peak table, click the
Next button. For shift to a specific peak in the
chromatogram, click the mouse on the peak.
The retention time indicated in the dialog box is changed to
that of the selected peak.
(3)
Identify the peak whose retention time is 4.9 minutes.

This is a peak of Asp. Enter Asp in the Peak name field.
For other items, retain their initial settings.
(4)
After completion of setup, proceed to identification of the next

peak. Click the Next button.
(5)
Repeat steps (3) and (4) until the last peak Arg is set up.
(6)
After input of all peaks, click the Done button.
(7)
at the upper part of

Click the Peak/Group Tables icon
the screen.
Make sure that the information set in steps (1) to (7) is input.
2 - 44
2.9.2
(8)
Drag the scroll box rightward. Then, enter nmol for Units
and select Calib for RT Update.
2 - 45
2.9
(9)
Further drag the scroll box rightward and select VIS 1 for
Analysis Channel. However, select VIS 2 only for Pro.
Select Linear for Fit Type (working curve type) and turn on
the check box of Zero (through-zero working curve) by
putting a check mark there.
(10) Enter 2 nmol (concentration of standard sample) for

Level 1.
It is possible to customize the peak table so that only the

parameters necessary for the specified calibration are
displayed. Refer to the user guide attached with EZChrom
Elite for the method of customizing the peak table, and the
details of each sequence.
2 - 46
2.9.2
(11) To save these settings, click File/Method/Save.

* For displaying peak information on the chromatogram, take
the following procedure.
(a) Click the right button of the mouse on the chromatogram
and select Annotations.
2 - 47
2.9
(b) From the Available Annotations list, select (highlight) an

item to be displayed and click the right arrow (green) or
double-click the item.
Select Name and Retention Time.
Other parameters should be set the same as those on
this screen.
(c) Select an item to be displayed and click the OK button.
2 - 48
2.9.2
Chromatogram with Peak Information (channel 1)
(d) Repeat steps (a) to (c) to make setting on channel 2 side

the same as on channel 1 side.
After selecting items to be displayed, click the OK button.
Chromatogram with Peak Information (channel 2)
2 - 49
2.10
2.10
Generation of Report
Generation of Report
2.10.1 Report Setup
EZChrom Elite is furnished with the standard report templates

usable for generating a report. Generate an external standard
method report using the template.
(1)
Click Method/Custom Report.
(2)
at the upper part of the screen and

Click the Open icon
select Open Report Template.
2 - 50
2.10.1
(3)
Select PH (small).srp as an example and click the Open

button.
(4)
In the external standard method report template, the

chromatogram and analytical report table can be
displayed/printed out as a report. When displaying the
chromatogram on this screen, it is unnecessary to specify the
data source and trace.
(5)
For displaying the contents of the analytical report table, click

the right button of the mouse on the layout and select Show
Data at Design Time.
For printing the generated report, click the Print icon
the custom report screen.
on
* When necessary, it is possible to modify the standard

report template or create a new report by use of the custom
report function. For details of custom report generation,
refer to the user guide furnished with EZChrom Elite.
(6)
Save the method in overwrite mode.

Click File/Method/Save.
(7)
Close all of the opened screens.

2 - 51
2.11
2.11
Sequence Run (execution of continuous analysis)

Described below is the procedure for creating a sequence table to
continuously measure one standard sample and four unknown
samples.
2.11.1 Creation and

Saving of
Sequence Table
(1)

Instrument Wizard.
(2)
Click the Create a sequence button.
(3)
to start up the
Make sure that the method to be used for measurement is

set in the Method field. L-8900 Test created in 2.7.1 to
2.7.4 is specified this time.
In the Data File Type area, select For acquisition.
following screen.

2 - 52
2.11.1
(4)
Make setting for each unknown sample.

Sample ID
Enter a sample ID. Select <Increment Number> and
<Method Name> by clicking the blue right arrow button.
Data path
Enter a path name for data saving or select an existing path
name by clicking the Open File button.
Data file
Select a Sample ID by clicking the blue right arrow button.
Number of unknown runs in sequence :
Repetitions per run
:
4
1

above screen.
(5)
2 - 53
2.11
(6)
Make setting for the Autosampler.

Unknown vials of sequence (First vial) : 2
Calibration vials of sequence (First vial) : 1
(Check the standard sample position in the sample rack and
enter a Vial No.)
Autosampler injection volume
: 20 L
(7)
2 - 54
2.11.1
In this dialog box, Calibration ID and Calibration File are

automatically set up the same as in the dialog box for the
unknown sample.
Set 1 for the number of calibration levels.
For other parameters, retain their initial settings.
(Make sure that they are set up the same as those on the
above screen.)
(8)

The sequence edit screen will open automatically.
(9)
Select the 1st line in the sequence table, click the right button
of the mouse and select Insert Line to add one line to the
sequence table.
2 - 55
2.11
(10) Add an RG process method.

Level
: 0
Vial
: 1
Volume (uL) : 0 (This must be set as 0 L.)
Method
: L-8900_Test_RG.met
NOTICE: 1. Make sure that the vial number is correct.
If an invalid vial number (invalid numerical
value or blank) is set, the run is stopped as
soon as the invalid value is recognized and
Valid vial numbers
Standard rack
: 1 to 200
Input in multi-rack use : Rack No. +
Vial No.
(example: A200)
2. Make sure that the autosampler injection
volume is proper. If an invalid injection
volume is set, the run is stopped as soon as
the invalid value is recognized and
2 - 56
2.11.1
(11) For performing a baseline check at the start of an analysis,

set Baseline Check as the Run Type.
Choose Continue as Action as in the case of NG.
(12) Click File/Sequence/Save As.

the name of L-8900 Test.
2 - 57
Save the sequence under
2.11
2.11.2 Execution of
Warm-up
Make sure that the Connect button is clicked on the Instrument

Status screen (L-8900 tab).
(Make sure that Idle is indicated in the System Status field.)
(1)
Click the Single Run

Acquisition dialog box.
(2)
button to open the Single Run
Method
: PH 4.6 60-2622(Stand-by).met
Vial
: 10
Injection volume : 0 L
Upon clicking the Start button, a single run is executed.
Open the Instrument Status screen and make sure that the
warming-up process is started.
System Status : Running (under warm-up)
Pump1
: ON
Pump2
: ON
Reactor Heater : ON
2 - 58
2.11.3
2.11.3 Execution of
Sequence Run
(addition of Run
Queue)
(1)
During execution of the warm-up process, click Sequence

Run
Make sure that L-8900 Test saved earlier is set in the

Sequence name field.
(2)
When it is desired to automatically print out reports after

measurement, turn on the check box of Print method reports
(by putting a check mark there). Reports on all the samples
contained in the sequence table are automatically printed out
in the template specified in Method Custom Report.
(Make sure that other parameters are set up the same as
those on the above screen.)
(3)
After completion of setup, click the Submit button.
(4)
2 - 59
2.11
(5)
Click Display Run Queue

screen.
at the upper part of the
Make sure that the sequence is added to Run Queue.

With the above operations, the sequence is added to Run
Queue and Sequence Run is executed after the warming-up
process.
First, the RG process method is executed.
System Status: Running (RG)
Next, the analytical method is executed.
System Status: Running (Acquisition Data)
Upon completion of measurement, the system transits to the
wash process automatically.
System Status: Running (Wash)
Upon completion of the wash process, the System Status
indication returns to Idle.
For turning ON the pump1, pump2 and reactor again; operate
the relevant buttons in the Manual Operation area of the
Instrument Status screen (L-8900 tab). The photometer
lamp is turned ON automatically by executing any of Preview
Run, Single Run and Sequence Run.
When the wash process is finished, you can safely power off
the L-8900. Before turning off power, click the Disconnect
button and make sure that Initialized is indicated in the
System Status field.
2 - 60
2.12.1
2.12
Sequence Processing
2.12.1 Check of
Working
Curve
After completion of measurement, check the created working

curve. For check, click Method/Review Calibration.
The calibrated peak is displayed in the peak list at the upper right
of the window. The displayed working curve is that of the
highlighted peak. By highlighting another peak, its working curve
can be displayed.
For detailed explanation of the working curve, refer to the user
guide furnished with EZChrom Elite.
2.12.2 Sequence
Processing
The sequence table is usable not only for continuous analysis, but
also for reprocessing of acquired data as a sequence of data.
Described below is the procedure for creating a sequence table for
analysis by use of acquired data (1 standard sample and 4
unknown samples) and carrying out reprocessing.
2 - 61
2.12
Sequence Processing
2.12.3 Creation of
Sequence
Table for
Reprocessing
Create a sequence table for reprocessing by use of the Sequence

Wizard.
(1)

Instrument Wizard.
(2)
Click the Create a sequence.
(3)
to start up the
Make sure that L-8900 Test is selected for the method.

For creating a sequence table for processing by use of
existing data, select From existing data files as the type of
data file.
2 - 62
2.12.3
(4)
Click the Open File button and select data to be included in

the sequence.
Given below is the procedure for selecting data of one
standard sample and four unknown samples measured in
2.11 and creating a sequence table.
2 - 63
2.12
Sequence Processing
(5)
For data file selection, select (highlight) the file name and
click the Add button. When the file list is completed, click
the Open button to return to the Sequence Wizard.
(6)

automatically.
(7)
For creating a working curve, enter the number of calibration

levels for the sample whose data has already been acquired.
Enter 1 for Level here. The Run Type is then changed
from Unknown to Calibration.
2 - 64
The sequence edit window will start
2.12.3
* Setting Procedure
in the Run Type field for the
(a) Click the arrow button
first sample in the calibration set.
(b) The Sample Run Type(s) dialog box for the sample will
appear.
(c) Select Clear All Calibration and click the OK button.
(d) Make sure that CAL CCA is indicated in Run Type.

For details of sample run type setting, refer to the user
guide furnished with EZChrom Elite.
2 - 65
2.12
Sequence Processing
(8)
Save the sequence.
Click File/Sequence/Save As.
Enter L-8900 TEST Recalc in the File name field and click
the Save button.
2.12.4 Execution of
Sequence Run
(1)
at the upper part of

Click the Sequence Process icon
the screen. The following screen will appear.
2 - 66
2.12.4
(2)
Make sure that L-8900 TEST Recalc saved earlier is set in

the Sequence name field. For creation of a working curve or
determination of the unknown sample concentration, retain
the initial settings for other items. For check of processing
results or report printing, select the following option as
occasion demands.
Review
Selected for check of processing results.
Results review (pause after each run):
Selected for check of processing results for each data.
Calibration review (pause after each calibration set):
Selected for processing of calibration (working curve) data.
Printing
Selected for report printing.
Print method reports:
Selected for automatic printing of custom reports according to
the template specified in the method after completion of
processing.
Print sequence reports:
Selected for automatic printing of sequence reports selected
for the run type after processing.
(3)
After completion of setup, click the Start button.

With the above operations, sequence processing is executed.
2 - 67
2.13
2.13
Termination of EZChrom Elite for HITACHI
Termination of EZChrom Elite for HITACHI
2.13.1 Termination of
EZChrom Elite
(1)
When quitting EZChrom Elite, click File/Exit or the Close

icon
at the upper right of the screen.
(2)
To close the EZChrom Elite main screen, click File/Exit or

at the upper right of the screen.
the Close icon
2 - 68
2.13.2
(3)
2.13.2 Termination of
Windows XP
The confirmation dialog box will appear.

button to exit EZChrom Elite.
Click the Yes
For quitting Windows XP and powering off the PC, take the
(1)
Click the Start button and then Shut Down.
2 - 69
2.14
Shutdown of L-8900
(2)
2.13.3 Forced
Termination of
Program at
Occurrence of
Trouble
Upon selecting Shut Down and clicking OK, the termination

procedure is started.
If a communication error occurs or a certain screen cannot be

exited during execution of the EZChrom Elite program or another
program, do not power off the PC but terminate the program in the
(1)
Press the Ctrl, Alt and Delete keys simultaneously.

The Windows XP Security screen will appear. For forced
termination, click the Task Manager button.
Upon clicking the Applications tab, a list of programs under
execution appears.
(2)
2.14
Select an application to be terminated, and click the End

Task button.
Shutdown of L-8900
Turn off the POWER switch on the right side of the analyzer.
When the wash process is finished, you can safely power off the
L-8900. If EZChrom Elite for HITACHI is started, click the
Disconnect button, and make sure that Uninitialized is indicated
in the System Status field before turning off power.
2 - 70
2.15
2.15
Precautions on Measurements
(1)
Sequence Table Setting

For ensuring safety in stopping the instrument, the washing
process is automatically carried out on completion of the
sequence run, and thereafter, the shutdown sequence is
performed.
Therefore, even if Shutdown is specified at the Run Type
item of the Sequence Table, the shutdown function intrinsic
to the EZChrom Elite becomes ineffective.
(2)
Extension of Data Acquisition Time

To extend a data acquisition time during measurement, click
Control/Extend Run on the menu bar to open the Extend
Run dialog box. In this dialog box, enter a desired data
acquisition time.
Although the data acquisition time can thus be extended
according to the input in the Extend Run dialog box, the
Cycle Time and Run Time indications on the Instrument
Status screen remain unchanged. On the chromatogram
display, check the actual cycle time and run time concerned.
Note that if the data acquisition time is extended beyond the
cycle time, an adverse effect is given to data accuracy.
In common practice, therefore, specify a data acquisition time
so that it will not exceed the cycle time.
(3)
Addition of Run Queue

Upon completion of the sequence run, the washing process is
automatically called into execution and then the shutdown
sequence is performed. Therefore, even if a run queue is
added to the sequence, the next queue execution is not
performed. (Since the pump1, pump2, and reactor heater
are turned off due to execution of the washing process, an
error is issued at the start of the next sequence.)
To add a sequence or modify any existing sequence, edit the
sequence in execution without adding a run queue.
Note that carrying out a single run (Stand-by Method)
between sequences makes the next sequence measurement.
(The pump 1, pump 2, and reactor heater can be turned on
only in a case where the Stand-by Method is carried out by
means of a single run.)
2 - 71

L-8900 Instruction Manual For Software

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L-8900 Instruction Manual For Software

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INSTRUCTION MANUAL

Please read through this manual

This manual pertains to the main unit of Model

Hitachi High-Technologies Corporation

Part No. 852-9011-1 TG-TH (NH-LH)

Be sure to read through and understand the following points

ABOUT THIS MANUAL

Limitations and Exclusions on Warranty

Failure due to operation at a place not meeting the

Failure due to power supply voltage/frequency other

Corrosion or deterioration of the piping due to

Corrosion of the electric circuits or deterioration of the

Failure due to use of software, hardware or spare parts

Failure due to use not described in the manual or

Failure due to maintenance or repair by other than

Failure due to relocation or transport conducted not

Failure due to disassembly, modification or relocation

Failure due to acts of God, including fire, earthquake,

Failure of the hardware, or damage to the system

After disposal of this instrument, after its resale without

Installation, Relocation and After-Sale Technical Service

Installation and Relocation

Installation at delivery shall not be carried out by the

Before installation, the user shall make preparations for

If relocation becomes necessary after initial installation

For after-sales service, contact our sales

For service after the warranty period, consult us with

Technical Seminars and Training Courses for Users

Precautions on Disposal of Instrument

Although at present this instrument does not use materials

For disposal of waste solution, refer to the instruction manual

The user is responsible for following relevant legal standards

Chemicals shall be handled, stored and discarded as

Physiologically or biologically active samples are not

Installation, Maintenance, and Relocation

DANGER does not apply to this instrument.

WARNING does not apply to this instrument.

Cautions on Calculation of Reagent

OPERATION ............................................................................................................ 2-1

Startup of L-8900 ...................................................... 2-3

Waveform Processing and Peak Identification ...... 2-37

2.13.2 Termination of Windows XP ........................ 2-69

Construction of L-8900 .......................................................................................... 1-1

Setup of L-8900 ..................................................................................................... 1-5

Setup of Rack Parameters..................................................................................... 1-7

Setup of Column Information ................................................................................. 1-9

Setup of L-8900 Parameters.................................................................................. 1-15

Instrument Status Screen (L-8900 tab) .................................................................. 1-31

Autosampler Dialog Box........................................................................... 1-41

Instrument Status Screen (Option tab)................................................................... 1-51

Pump ON/OFF Dialog Box..................................................................................... 1-58

Error Message List................................................................................................. 1-60

1.10 Status Transition.................................................................................................... 1-71

Select File/New/Instrument in the main menu.

An instrument can be renamed:

Right-click on the instrument icon.

Click the drop-down list of Instrument type.

Move L-8900 icon to Configured modules from the

NOTE: The ? mark is attached to the L-8900 icon

Click the Auto Configuration button.

Click the OK button.

Right-click the L-8900 icon in the Configured modules box.

(10) Click the Options button.