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Thixotropyandrheopexyofmusclefibersprobedusingsinusoidaloscillatio...:EBSCOhost

UNIVERSIDADANTONIO
NARINO

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Thixotropyandrheopexyofmusclefibersprobedusing
sinusoidaloscillations.
Authors: AltmanDDepartmentofPhysics,WillametteUniversity,Salem,Oregon,United
StatesofAmerica.
MinozzoFCDepartmentofKinesiologyandPhysicalEducation,McGillUniversity,
Montreal,Quebec,Canada.
RassierDEDepartmentsofKinesiologyandPhysicalEducation,Physiology,and
Physics,McGillUniversity,Montreal,Quebec,Canada.
Source: PlosOne[PLoSOne]2015Apr16Vol.10(4),pp.e0121726.DateofElectronic
Publication:20150416(PrintPublication:2015).
PublicationType: JournalArticleResearchSupport,NonU.S.Gov't
Language: English
JournalInfo: Publisher:PublicLibraryofScienceCountryofPublication:UnitedStatesNLMID:
101285081PublicationModel:eCollectionCitedMedium:InternetISSN:19326203
(Electronic)LinkingISSN:19326203NLMISOAbbreviation:PLoSONESubsets:
MEDLINE
ImprintName(s): OriginalPublication:SanFrancisco,CA:PublicLibraryofScience
MeSHTerms: Muscle,Skeletal/*physiology
AnimalsRabbits
Abstract: Lengthchangesofmusclefibershavepreviouslybeenshowntoresultina
temporaryreductioninfiberstiffnessthatisreferredtoasthixotropy.
Understandingthemechanismofthisthixotropyisimportanttoourunderstanding
ofmusclefunctionsincetherearemanyinstancesinwhichmuscleissubjectedto
repeatedpatternsoflengtheningandshortening.Byapplyingsinusoidallength
changestooneendofsinglepermeabilizedmusclefibersandmeasuringtheforce
responseattheoppositeend,westudiedthehistorydependentstiffnessofboth
relaxedandactivatedmusclefibers.Forlengthchangeoscillationsgreaterthan1
Hz,weobservedthixotropicbehaviorofactivatedfibers.Treatmentofthesefibers
withEDTAandblebbistatin,whichinhibitsmyosinactininteractions,quashedthis
effect,suggestingthatthemechanismofmusclefiberthixotropyiscrossbridge
dependent.Wemodeledahalfsarcomereexperiencingsinusoidallengthchanges,
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Thixotropyandrheopexyofmusclefibersprobedusingsinusoidaloscillatio...:EBSCOhost

andoursimulationssuggestthatthixotropycouldarisefromforcedependent
crossbridgekinetics.Surprisingly,wealsoobservedthat,forlengthchange
oscillationslessthan1Hz,themusclefiberexhibitedrheopexy.Inotherwords,the
stiffnessofthefiberincreasedinresponsetothelengthchanges.Blebbistatinand
EDTAdidnotdisrupttherheopecticbehavior,suggestingthatanoncrossbridge
mechanismcontributestothisphenomenon.
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Molecular DryadJF35G
Sequence:
EntryDate(s): DateCreated:20150417DateCompleted:20160108LatestRevision:20150422
UpdateCode: 20160108
PubMedCentral PMC4400131
ID:
DOI: 10.1371/journal.pone.0121726
PMID: 25880774

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