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(gene expression)
RNA
Transcription
Protein
Translation
Producing RNA
involves
chromatin
dynamics
DNA methylation
Methyl groups added to
certain DNA bases repress
gene activity.
Histone modification
A combination of different
molecules can attach to the
tails of proteins called
histones. These alter the
activity of the DNA wrapped
around them.
Compact
chromatin
Goals and timing of DNA and RNA synthesis are very different
RNA synthesis is very dynamically regulated
Making many copies is a way to amplify the information present in DNA
RNA molecules
Transfer RNA
Ribosomal RNA
Messenger RNA
Methyl
group
RNA
Ribonucleic acid
A molecule also made up
of nucleotides linked
together by covalent
bonds
DNA is transcribed into
RNA using the SAME
RULES of nucleotide
base pairing used by
DNA, except that Uracil
is used
H!
H!
H!
An RNA Nucleotide
Ribose, a monosaccharide
A phosphate group (PO4)
A nitrogenous base (either adenine,
guanine, cytosine, or uracil)
RIBONUCLEOTIDES,
NOT DEOXYRIBO
NUCLEOTIDES
RNA Transcription
Definition: the synthesis of RNA, using DNA
as a template
Occurs during interphase within the nucleus
Enzymes cause the DNA double-helix to uncoil
and straighten out
The weak hydrogen bonds between the base
pairs break apart (the molecule unzips )
One of the two strands of nucleotides is used as
the DNA coding strand; the nitrogenous
bases of this strand bond with new RNA bases
within the nucleus to form a strand of RNA
RNA polymerase II
transcription-initiation complex
Initiation by Pol II requires general
transcription factors, which position Pol II at
initiation sites and are required for
transcription of most genes transcribed by this
polymerase
General transcription factors are multimeric
Proteins comprising the Pol II transcriptioninitiation complex assemble in a specific order
in vitro
DNA
Control of transcription
Transcription start site usually a TATA
box (not always)
TBP (TATA-binding protein) binds,
changing DNA structure
Recruits transcription factor II proteins
(TFIIA, B, ) then RNA Pol II
Collectively known as the pre-initiation
complex or the transcription initiation
complex
RNA synthesized
Release of
transcription factors
The basic
mechanism of
transcribing DNA
into RNA!
Processing of Transcripts
Capping of 5 end of RNA
Poly-adenylate 3 end (add Poly A tail):
Poly A polymerase adds 100 s of
adenosines (A s) to end of transcript;
length of poly A tail influences half-life of
RNA (degradation rate)
Splice out intervening sequences
(introns), leaving expressed sequences
(exons)
Unique linkage!
recognized by special factors!
not susceptible to nucleases!
Poly A tail
RNA transcripts contain AAUAAA site at
the 3 end
RNA is cleaved 10-36 nucleotides
downstream of the AAUAA site
Poly(A) polymerase adds a tail of 100-200
adenosines at the 3 end of the cleaved
RNA strand, just passed the AAUAA site
Poly A tail used to protect mRNA from
degradation
RNA splicing
Introns are removed by spliceosomes
Branch site
(Leader)
(Trailer)
Gene Regions
TRANSCRIPTION
INITIATION
PROMOTER
ATG
START
CODON
GENE
TRANSCRIPTION
TERMINATION
TAA
TAG
TGA
TERMINATOR
STOP
CODONS
Promoter Region!
Enhancer
Promoter-proximal region
Promoter
TATA
Box
(-30 bp)
Start
point
Template
DNA strand
ENHANCERS
Enhanceosome
TATA and CAAT boxes are located at about the same positions in most
promoters. The GC and octamer boxes may or may not be present; when
present, they occur at different location, either singly or in multiple copies.
Goals and timing of DNA and RNA synthesis are very different
RNA synthesis is very dynamically regulated
Making many copies is a way to amplify the information present in DNA