International Journal of Pharmacological Research

ISSN: 2277-3312

www.ssjournals.com
Journal DOI:10.7439/ijpr

Anti hyperlipidemic activity of Tephrosia purpurea plant extracts in

poloxomer 407 induced hyperlipidemic rats
Pankti P. Dalwadi* and Pragnesh V. Patani
Department of Pharmacology, Arihant School of Pharmacy & Bio-Research Institute, Gujarat, India

Corresponding author*
Dalwadi Pankti P. M. Pharm,
Research Scholar,
Department of Pharmacology,
Arihant School of Pharmacy & Bio-Research Institute,
Uvarsad Cross Road, Sarkhej-Gandhinagar Highway, Adalaj. Gujarat, India
E-mail: dalwadipankti2034@gmail.com

Abstract
Aim & Objective: Evaluation Of Antihyperlipidemic Activity of Tephrosia purpurea Plant Extracts.
Material & Method: Screening of antihyperlipidemic was done by using Tephrosia purpurea Leaves, Stem and Whole
plant (except Leaves) extracts. Part extracts used for antihyperlipidemic activity were stem (500 mg/kg), leaves
(400mg/kg) and whole plant extracts except leaves at the dose of (300mg/kg, 600mg/kg).Hyperlipidemia was
administered in experimental animals using Poloxomer 407 by intraperitoneal administration at the dose of 1g/kg. Blood
was withdrawn through retro orbital puncture at 15& 24 hrs intervals. Antihyperlipidemic activity of Tephrosia purpurea
plant extracts were evaluated by estimation of lipid profiles.
Results: Estimation of lipid profile shows that Tephrosia purpurea stem extract (500mg/kg), Tephrosia purpurea leaves
extract (400mg/kg), Tephrosia purpurea whole plant extract (300mg/kg) shows the less significant antihyperlipidemic
activity. While Tephrosia purpurea whole plant extract at the dose of 600mg/kg shows potent antihyperlipidemic activity.
It decreases TC, TG, LDL, VLDL and increases HDL levels. Tephrosia purpurea whole plant extract 600 mg/kg shows
significant antihyperlipidemic activity as standard drug Atorvastatin.
Conclusion: Hydromethanolic extracts of Tephrosia purpurea whole plant inhibits cholesterol and triglycerides
synthesis. Increasing in stimulation of lipolysis by lipoprotein lipase is responsible for plasma triglycerides hydrolysis.
The elevation of serum cholesterol levels following i.p. injection of Poloxomer 407 solution to rats was due to
stimulation of 3-hydroxy-3-rmethylglutaryl-Co-enzyme A (HMG-Co A) reductase activity in the liver by the Poloxomer.
Hydromethanolic extract of Tephrosia purpurea Showed lowering of TG levels by increasing the lipoprotein lipase
activities. The cholesterol lowering effect of Tephrosia purpurea extract were observed in rats treated with the
Poloxomer 407 by the inhibition of HMG Co A reductase inhibition.
Keywords: Hyperlipidemia, Coronary Heart Disease, Poloxomer 407, Triglycerides, Tephrosia purpurea, Atorvastatin

1. Introduction
Hyperlipidemia is a major cause of atherosclerosis and the atherosclerosis-associated conditions, such as coronary
heart disease, ischemic cerebrovascular disease and peripheral vascular disease. Although the incidence of the
atherosclerosis related events has declined in the united states, these condition still accounts for the majority of morbidit y
and mortality among middle aged and older adults, the incidence and absolute number of annual events will increase over
the next decade because of epidemic of obesity and ageing of the U.S population. 1,2
Dyslipidemia, including hyperlipidemia and hypercholesterolemia and low level of high density of lipoproteins
cholesterol HDL are major cause of increased atherogenic risk; both genetic disorders and lifestyle diet high in calories,
saturated fat, and cholesterol contribute to dyslipidemia seen in developed countries around the world. 3 Severe
hypertriglyceridemia (i.e. Triglyceride level of >1000mg/dl) requires therapy to prevent pancreatitis. 4 Moderately elevated
triglyceride level 150 to 400mg/dl also are concern because they often occur as part of the metabolic syndrome, which
includes insulin resistance, obesity, hypertension, low HDL level and substantially increased CHD risk.5,6 Medicinal plant
based drug has now advantageous over modern drugs. As such are long history of use and better patient tolerance as well
as public acceptance, renewable source cultivation and processing environmental friendly, local availability, plant may
major source of lead generation. Several recent break through are gugulipid, taxol, artimesinin. 7Medicinal plant contains
so many chemical compounds which are the major source of therapeutic agents to cure human disease.8
Tephrosia purpurea is commonly known in English as wild indigo and in Gujarati Ghodakan. Tephrosia
purpurea is belonging to family leguminoceae.9 Tephrosia purpurea is a self generating erect or spreading perennial herb
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found throughout India. It can be found as an ingredient in traditional herbal formulations. Whole plant may be used for its
rich flavonoids and polyphenol content.10 Tephrosia purpurea traditionally used to cure several types of external wounds
and gastro-duodenal disorders.11 medicinal plant is used in cough, tightness of chest. Decoction of root is useful in
enlargement and obstruction of liver, spleen and kidney12, also used for dyspepsia and chronic diarrhea. 13 Gargle of
Tephrosia purpurea is used to wash out mouth. Root is also used in inflammation, skin disorders, elephantitis, flatulence,
hemorrhoids, asthma, bronchitis, anaemia, dysmenorrhoea, chronic fever, boils, pimples, gingivitis. 14 Whole plant has been
used to cure tumors, ulcers, leprosy, allergic and inflammatory condition such as rheumatism asthma and bronchitis an
extract of pods is effective as analgesic, anti-inflammatory, and their decoction is used in vomiting like symptoms. 15,16
Reported activities of Tephrosia purpurea leaves posses spasmolytics,17 anti hyperglycemic and antilipidperoxidative,18
antioxidant,19 antipyretic20, anticancer21 Antihyperlipidemic, 22 anthelmintic.23 Whole part of Tephrosia purpurea posses
antilesishmania,24 anti epilepsy,25 anxiolytic,26 diuretic,27 anti diarrheal 28activities. Tephrosia purpurea contains Tephrosine
A, Tephrosine B,29,30 Purpurine, Rotenone,31 Spinasterol, -sitosterol, Karanjin, Pongaglabol, Pongamol, 32 Semiglabrin,
isolonchocarpin33.

2. Material & Method

2.1 Drugs & chemical
Poloxomer 407 was procured from Emcure Pharmaceutical Ltd Ahmedabad, estimation of Total cholesterol;
Triglycerides, HDL, LDL and VLDL were done using span diagnostic kit. Atorvastatin was procured from Emcure
Pharmaceutical Ltd.
2.2 Plant material
Fresh whole plant of Tephrosia purpurea was procured from Jawaharlal Nehru Government Medicinal botanical
garden Gandhinagar. Tephrosia purpurea whole plant was authenticated by department of genetics & plant breeding, B.A
college of agriculture, Anand Agriculture University with authentication no BACA/GPB/651/14 also the leaves and stem
were collected. The collected parts of the plant were washed properly and dried in shed and powdered with mechanical
grinder to make coarse powder. The resulting powder was used for extraction
2.3 Extraction procedure of Tephrosia purpurea
Fresh leaves, stem and whole plant except leaves were collected, shed dried for 7 days and coarsely powdered
with mechanical grinder passed through sieve no 40. 100g of powder packed into column and was extracted with
Methanol: Water (60:40) at 60 c using Soxhlet apparatus for hydromethanolic mixture of Tephrosia purpurea plant
extracts Hydromethanolic mixture was concentrated using rotary evaporator under reduced pressure, concentrated thick
extract of leaves, stem and whole plant except leaves were obtained. 34
2.4 Selection of doses for the study
Dose of Tephrosia purpurea was selected from the acute toxicity study of Tephrosia purpurea. LD50 is the dose
which is lethal to 50% of a population. LD50 of Hydromethanolic extract of Tephrosia purpurea was already reported at
2000 mg/kg.35 For current study the dose was selected based on LD50 of whole plant except leaves extract. So the dose of
leaf extract was 400 mg/kg, stem extract was 500 mg/kg and doses for whole plant were 300 mg/kg and 600 mg/kg.
2.5 Experimental Animals
The complete experiment was carried out using 42 wistar rats of albino species of weighing between 180-200.
Institutional animal ethical committee (IAEC) approved the experimental protocol number ASP&BRI/AH/2014/02 Rats
were use as it accumulates cholesterol in liver and blood as faster than mouse. They were housed under standard laboratory
condition at room temperature 372C along with relative humidity 45- 55%, 12/12 dark and light cycle in an animal
house approved by committee for the purpose of control and supervision of experiments on animals (CPCSEA).they were
treated with standard pelleted diet and ad libitum and housed in propylene cages. After seven days of acclimatization
period, they were allowed to use for experimental work.
2.6 Anti hyperlipidemic study
Antihyperlipidemic studies were carried out and total cholesterol, triglycerides, HDL, LDL and VLDL level in the
blood were checked.
2.7 Induction of hyperlipidemia
Poloxomer 407 is non ionic hydrophobic surfactant. A colloidal solution of Poloxomer 407 was prepared by
dissolving 3.5 g in 26 ml of cold normal saline (0.13 g/ml), which was stored at regular freeze temperature 4 C. Each six
animals of seven groups treated with Poloxomer 407 intraperitoneally to the animals very carefully at the dose of 1 g/kg
Tephrosia purpurea extracts were administered orally at various doses to evaluate anti hyperlipidemic activity of the test
extracts. The results were compared with the standard drug Atorvastatin 10 mg/kg was administered orally. 36,37
2.8 Collection of blood samples & Experimental set up
Blood samples (0.2 ml) was collected serially through retro orbital puncture at 15 & 24 hrs. 38 After leaving the
blood to clot for 30 min at room temperature, serum will separate by centrifugation. Lipid profile of sample was calculated
using diagnostic kits. Test doses of various parts of plants were evaluated for antihyperlipidemic activity. The rats were
divided into 7 groups having 6 animals in each group as follows:

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Group I
Group II
Group III
Group IV
Group V
Group VI

:Normal Control (saline solution, p.o)

:Hyperlipidemic control (1 g/kg of Poloxomer 407 as per b.w, Once day, i.p.)
:Positive control, (10 mg/kg of Atorvastatin as as per b.w, once day, p.o)39
:Hydromethanolic extract of Tephrosia purpurea leaves (400 mg/kg as per b.w, once day, p.o)
:Hydromethanolic extract of Tephrosia purpurea stem (500 mg/kg as per b.w, once day, p.o)
:Hydromethanolic extract of Tephrosia purpurea whole plant except leaves (300 mg/kg as per b.w, once day,
p.o)
Group
:Hydromethanolic extract of Tephrosia purpurea whole plant except leaves (600 mg/kg as per b.w, once day,
VII
p.o)
2.9 Estimation of blood cholesterol and lipid profile:
Total cholesterol estimation was done by using the span total cholesterol diagnostic kit. Serum triglycerides and
HDL were estimated by span triglycerides diagnostic kit. Cholesterol, triglycerides and HDL profile were estimated using
standard monograph.
LDL cholesterol was calculated using formula
LDL = Total Cholesterol - HDL - Triglycerides/5
VLDL was calculated using the formula
VLDL = Triglycerides/5
2.10 Statistical Analysis
The results were expressed as Mean SEM ,The statistical analysis involving all seven groups were carried out
by analysis of variance(ANOVA) followed by Tukeys test ,where P value < 0.05 is considered as statistically significant.
Data were processed with graph pad prism version 6.00 software

3. Results
3.1 Phyto chemical screening of Tephrosia purpurea extracts:
Photochemical screening of Tephrosia purpurea plant extracts shows the presence of glycosides, flavanoids
alkaloids, saponins, phenolic and carbohydrates.
3.2 Effect of Tephrosia purpurea extracts on total cholesterol & lipid profile
Poloxomer 407 administration caused acute hyperlipidemia in rats where it significantly increased total
cholesterol, TG and decreased in HDL in rats treated with Poloxomer 407 group as compared to normal control group
(p<0.001).
Table 1: Effect of Hydromethanolic extracts of Tephrosia purpurea on serum lipid profile at 15 hrs after Poloxomer
407 induced hyperlipidemia
TC
TG
HDL
LDL
VLDL
Group
(mg/dl)
(mg/dl)
(mg/dl)
(mg/dl)
(mg/dl)
Control
77.654.01
86.945.23
37.722.39
22.555.23
17.391.04
Hyperlipidemic control 171.22.8$$$
168.47.01$$$ 28.622.81
108.95.48$$$
33.681.40$$$
TPSE
150.83.27*
117.64.15*
29.762.43*
97.524.73
23..520.85*
TPLE
145.93.20*
116.45.51*
31.822.93** 90.8 4.51
23.281.10*
TPWPE1
135.43.90**
111.23.39*
32.852.56*
80.314.47*
22.240.67*
TPWPE2
99.463.76***
87.603.20*
37.262.78** 44.685.12*** 25.440.78*
Atorvastatin
91.623.29**** 84.604.30** 38.22.40*** 45.284.82**
16.920.86**
TPSE: Tephrosia purpurea stem extract (400mg/kg); TPLE: Tephrosia purpurea leaves extract (500mg/kg); TPWPE1: Tephrosia
purpurea whole plant extract 1(300mg/kg); TPWPE1: Tephrosia purpurea whole plant extract 2(600mg/kg)

Table 2: Effect of Hydromethanolic extracts of Tephrosia purpurea on serum lipid profile at 24 hrs after Poloxomer
407 induced hyperlipidemia
TC
TG
HDL
LDL
VLDL
Group
(mg/dl)
(mg/dl)
(mg/dl)
(mg/dl)
(mg/dl)
Control
78.884.10
83.184.47
37.922.29
24.325.01
16.640.89
Hyperlipidemic control 177.63.98$$$
179.49.07$$$ 25.72.59
116 6.4$$$
35.881.94$$$
TPSE
156.43.39*
141.62.78*
33.01.34*
95.065.14
28.320.55*
TPLE
154.23.26*
139.43.95
35.714.39
90.56.20
27.80.79
TPWPE1
146.14.20**
146.14.20
38.094.91
82.578.12
25.440.78
TPWPE2
113.92.73***
119.42.87*
41.73.08*
48.285.44*** 17.520.64*
Atorvastatin
94.353.26**** 1153.11**
41.12.23*** 49.864.21*** 23 0.62**
Note: Values are expressed as MEAN SEM (n=6) one way ANOVA followed by Tukeys test
*p<0.05: significant when compared to disease control group; **p<0.01: significant when compared to disease control group;
***p<0.001: highly significant when compared to disease control group; ****p<0.0001: highly significant when compared to disease
control group; $$$p<0.001: highly significant when compared to control group

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Graph 1: Effect of hydromethanolic extracts of Tephrosia purpurea on Total cholesterol (mg/dl)

200

$$$

c o n tro l

$$$

150

D is e a s e c o n t r o l

**

**
****
***

****
***

100

TPSE
TPLE
TPW PE1
TPW PE2

50

S ta n d a rd

se

co
nt
c o ro l
nt
ro
TP l
SE
T
TP PL
W E
TP PE
W 1
St
P
an E2
da
rd

co
nt
c o ro l
nt
ro
TP l
SE
T
TP PL
W E
TP PE
W 1
St
P
an E2
da
rd

ea
is
D

is

ea

se

T O T A L C H O L E S T E R O L (m g /d L )

P O L O X O M E R 4 0 7 IN D U C E D H Y P E R L IP ID E M IA

15 H R S

24 H R S

T IM E IN T E R V A L ( H O U R S )

Estimation of serum total cholesterol was done at 15 & 24 hrs after induction of hyperlipidemia by Poloxomer
407. At 15 hrs normal control rats had Mean SEM of serum cholesterol level was 77.65 4.01 and at 24 hrs 78.88
4.10.Where the total cholesterol levels of rats treated with Poloxomer 407 had total cholesterol at 15 & 24 hrs were 171.2
2.81, 177.6 3.98. It Showed the significantly increases in total cholesterol levels in disease control. On 24 hrs total
cholesterol level of rats treated with TPWPE at the dose of 600mg/kg was 113.9 2.73 Showed significantly decrease in
total cholesterol levels as compared to disease control group. While in case of rats treated with of standard drug
Atorvastatin Mean SEM was found to be 94.33.26 at 24 hrs. TPWPE at the dose of 600 mg/kg was Showed potent
antihyperlipidemic activity as compared to other extracts.
Graph 2: Effect of hydromethanolic extracts of Tephrosia purpurea on Triglycerides (mg/dl)
P O L O X O M E R 4 0 7 I N D U C E D H Y P E R L I P I D E M IA

T rig ly c e rid e s (m g /d L )

200

$$$

C o n tro l

$$$

D is e a s e c o n t r o l
*

150

TPSE
*

100

*
*

**

TPLE
TPW PE1

**

TPW PE2
50

S ta n d a rd

on
t
c o ro l
nt
ro
TP l
SE
T
TP PL
W E
TP PE
1
W
St PE
an
2
da
rd

ea
is
15 H R S

is

ea

se

se

on
t
c o ro l
nt
ro
TP l
SE
T
TP PL
W E
TP PE
1
W
St PE
an
2
da
rd

24 H R S

T IM E IN T E R V A L ( H O U R S )

Triglyceride level is increased in rats treated with Poloxomer 407. At 15 hrs normal control rats had Mean SEM
of was 86.94 5.23and at 24 hrs 83.18 4.47.where the triglyceride levels of the rats treated with Poloxomer 407 at 15 &
24 hrs were 168.4 7.01,179.4 9.07 Showed the significantly increases in triglyceride levels in disease control. On 24
hrs triglyceride level of TPWPE at the dose of 600 mg/kg were 119.4 2.87 Showed significantly decrease in triglyceride
levels as compared to rats treated with Poloxomer 407 control group. While in case of rats treated with standard drug
Atorvastatin Mean SEM were found to be 115.3.11 at 24 hrs.

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Graph 3: Effect of hydromethanolic extracts of Tephrosia purpurea on HDL (mg/dl)

P O L O X O M E R 4 0 7 I N D U C E D H Y P E R L IP I D E M IA
50
* ***
** ***

H D L (m g /d L )

40
*

**

C o n tro l
D is e a s e c o n tr o l

TPSE

30

TPLE
TPW PE1

20

TPW PE2
S ta n d a r d

10

Di

Di

se

se

as

as

Co
e c n tr
on ol
tr
ol
TP
SE
T
TP PL
W E
TP PE
1
W
St PE
2
an
da
rd

Co
nt
co r ol
nt
ro
TP l
SE
T
TP PL
W E
TP PE
1
W
P
St
an E2
da
rd

15 H R S

24 H R S

T IM E IN T E R V A L ( H O U R S )

Serum HDL level at 15 hrs normal control rats had Mean SEM of was 37.7 2.39 and at 24 hrs 37.92 2.29
where the HDL levels of the rats treated with Poloxomer 407 at 15 & 24 hrs were 28.62 2.81, 25.7 2.59, Showed the
significantly decreases in HDL levels in disease control. On 24 hrs HDL level of the rat treated with Tephrosia purpurea
whole plant extract at the dose of 600mg/kg were 41.73.08 ,Showed significantly increase in HDL levels as compared to
disease control group. While in case of rats treated with standard drug Atorvastatin Mean SEM was found to be
41.12.35at 24 hrs.
Graph 4: Effect of hydromethanolic extracts of Tephrosia purpurea on LDL (mg/dl)
P O L O X O M E R 4 0 7 I N D U C E D H Y P E R L I P I D E M IA
150

c o n tro l
$$$

L D L m g /d L

$$$

D is e a s e c o n t r o l

100

TPSE
TPLE
TPW PE1
***

50

**

***

***

TPW PE2
S ta n d a rd

co
nt
c o ro l
nt
ro
TP l
SE
T
TP PL
W E
TP PE
1
W
St
P
an E2
da
rd
e

se
15 H R S

Di

se
Di

as

co

as

co

nt
ro
nt l
ro
TP l
SE
T
TP PL
W E
TP PE
1
W
St
P
an E2
da
rd

24 H R S

T IM E IN T E R V A L ( H O U R S )

LDL level at 15 hrs normal control rats had Mean SEM of was 25.5 5.23 and at 24 hrs 24.32 5.01.where
the LDL levels at 15 & 24 hrs were 108.9 5.48,116 6.4Showed the significantly increases in LDL levels in disease
control. On 24 hrs LDL level of the rats treated with Tephrosia purpurea whole plant extract at the dose of 600mg/kg were
48.25.4,it Showed significantly decrease in LDL levels as compared to disease control group. While in case of rats
treated with standard drug Atorvastatin Mean SEM was found to be 49.864.21 at 24hrs

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Graph 5: Effect of hydromethanolic extracts of Tephrosia purpurea on VLDL (mg/dl)

P O L O X O M E R 4 0 7 IN D U C E D H Y P E R L IP ID E M IA
40

$$$

c o n tro l

V L D L m g /d L

$$$

D is e a s e c o n t r o l

30
*

20

*
*
*

TPSE
**

TPLE
TPW PE1

**

TPW PE2
10

S ta n d a rd

co
nt
c o ro l
nt
ro
TP l
SE
T
TP PL
W E
TP PE
W 1
St PE
an
2
da
rd
ea
is

is

ea

se

se

co
nt
c o ro l
nt
ro
TP l
S
T E
TP PL
E
W
TP PE
1
W
St PE
an
2
da
rd

15 H R S

24 H R S

T IM E IN T E R V A L ( H O U R S )

VLDL level at 15 hrs normal control rats had Mean SEM of was17.39 1.04 and at 24 hrs 16.6 0.89.where the
VLDL levels of the rats treated with Poloxomer 407 at 15 & 24 hrs were 33.68 1.40,35.88 1.94, it Showed the
significantly increases in VLDL levels in disease control. On 24 hrs VLDL level of rats treated with TPWPE at the dose of
600mg/kg were 17.520.60 Showed significantly decreased in VLDL levels as compared to disease control group. While
in case of rats treated with standard drug Atorvastatin Mean SEM was found to be 23.50.62 at 24 hrs.
All the results were shows statistical significant (p<0.05) and in comparison to control and disease control group.
So from among study shows Tephrosia purpurea whole plant extract at 600 mg/kg shows significantly potent
antihyperlipidemic activity. While Tephrosia purpurea leaves, stem and whole plant at 300 mg/kg shows less potent
antihyperlipidemic activity.

4. Discussion
Objective of the present study was to screen the antihyperlipidemic activity of Tephrosia purpurea plant extracts.
It has been well established that nutrition play an important role in etiology of hyperlipidemia In present study, Poloxomer
407 induced model shows significant increase in TC, TG, HDL levels in rats treated with Poloxomer 407 group as
compared to normal control group (p<0.001). when we compared rats treated with Poloxomer 407 group was compared
with all rats treated with the Tephrosia purpurea extracts groups. It shows that Tephrosia purpurea whole plant extract at
the dose of 600mg/kg significantly decreases the TC (P<0.05), TG (P<0.05), while increases the level of HDL (P<0.001)
as compared to group treated with the Poloxomer 407. When we compared the rats treated with standard drug Atorvastatin
(10mg/kg) it shows significant increases in the level of HDL (p<0.001) as compared to Poloxomer 407 treated group.
Where Tephrosia purpurea whole plant extract at the dose of 600 mg/kg decreased TC (P<0.05), TG (P<0.01) and shows
significantly increase in HDL (P<0.001). The result demonstrate that Poloxomer induced experimental model used to
investigate the anti hyperlipidemic activity of Tephrosia purpurea.
Treatment of hyperlipidemia induced by Poloxomer 407 shows beneficial treatment by Tephrosia purpurea.
Hyperlipidemia characterized by abnormally elevated serum triglyceride (TG), total cholesterol (TC), LDL and VLDL, is
an established risk factor for the development of coronary artery disease (CAD). In the present study hydromethanolic
extracts of Tephrosia purpurea evaluated followed by i.p. administration of Poloxomer 407. Poloxomer 407 has been
utilized in the hyperlipidemic model due to its convenience, reproducibility and the lack of undesirable underlying
pathological conditions. Increased serum total cholesterol (TC), triglycerides (TG) and low density lipoprotein cholesterol
(LDL) levels are important risk factors for atherosclerosis development. On the other hand, elevated levels of HDL exert
an anti-atherogenic effect by counteracting LDL oxidation and facilitating the translocation of cholesterol from peripheral
tissue such as arterial walls to the liver for catabolism. Oral administration of stem, leaves and whole plant extracts of
Tephrosia purpurea significantly reduces TC, TG, LDL and VLDL and significantly increases HDL level. The result shows
that hydromethanolic extracts of Tephrosia purpurea of the plant have ability to reduce the risk of hyperlipidemia.
Poloxomer 407 is a hydrophilic non ionic surfactant. Poloxomer 407 induced hyperlipidemia is associated with
alteration in the activities of enzyme responsible for cholesterol transport and metabolism. Poloxomer 407 cause
significant increases in triglycerides level by the inhibiting capillary lipoprotein lipase which is responsible for plasma
triglycerides hydrolysis. Thus the rats treated with Poloxomer 407 shows significant increases triglycerides level followed
by i.p. administration of Poloxomer 407.atorvastatin is a HMG Co A reductase inhibitor which is used as standard drug.
The elevation of serum cholesterol levels following i.p. Injection of Poloxomer 407 solution to rats was due to stimulation
of 3-hydroxy-3-rmethylglutaryl-Co-enzyme A (HMG-Co A) reductase activity in the liver by the Poloxomer.
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Hydromethanolic extract of Tephrosia purpurea shows lowering of TG levels by increasing the lipoprotein lipase
activities. The cholesterol lowering effect of Tephrosia purpurea extract were observed in rats treated with the Poloxomer
407 by the inhibition of HMG Co A reductase inhibition.
The result of phytochemical investigation have lead to the conclusion that hydromethanolic extracts of Tephrosia
purpurea plant contains alkaloids, flavonoids, glycosides, saponins, phenolic and carbohydrate content. From the literature
review of antihyperlipidemic activity of herbal plants suggested that saponins and flavonoids content may be responsible
for lowering of TC, TG, LDL and VLDL levels and reduces the cardiovascular disease by increasing HDL levels.
Tephrosia purpurea stem (500mg/kg), leaves (400mg/kg) and whole plant extract (300mg/kg) were shows less
potent antihyperlipidemic activity. Whole plant extract at the dose of 600 mg/kg shows potent antihyperlipidemic activity

5. Conclusion
Hydromethanolic extract of Tephrosia purpurea whole plant have ability to reduced triglycerides levels.
Tephrosia purpurea have ability to restore the TG. By stimulating lipolytic activity of plasma lipoprotein lipase and
reduction in oxidative stress may be responsible for the antihyperlipidemic activity. However from the literature review
hypothesized those medicinal plants containing flavonoids may responsible for antihyperlipidemic activity. From the
phytochemical screening of Tephrosia purpurea whole plant extract shows the presence of flavonoids, glycosides, phenolic
component, saponins and alkaloids and carbohydrate. Further screening of individual active component is requires to
determine exact component of whole plant extract of Tephrosia purpurea responsible for antihyperlipidemic activity.

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