Beruflich Dokumente
Kultur Dokumente
PARASITES
UNICELLULAR
PARASITOLOGY REVIEW
PHYLUM
SUBPHYLUM
CHARACTERISTICS
Sarcomastigophora Sarcodina
Single celled
(amoeba)
Move by pseudopodia
T and C stages
Asexual reproduction
Mastigophora Same as Sarcodina
(flagellates)
But move by flagella
Some have T stage only
Blood and tissue
flagellates (APET)
MEDICALLY IMPORTANT
PARASITES
AMOEBA
UNICELLULAR
PHYLUM
Ciliaphora (ciliates)
CHARACTERISTICS
Single celled
Target tissue and blood cells
T and C stages
Asexual reproduction
Apicomplexa (sporozoa)
Entameoba histolytica
CYST
1-4 nuclei
4 nuclei in mature cyst
Cental karyosome/bulls eye
karyosome
Cigar-shaped chromatoidal
bodies
TROPHOZOITE
ONE nucleus (all trophozoite
uncer Sarcodina)
Fine, peripheral chromatin
Cental karyosome/bulls eye
karyosome
Ingested RBC (none for E.
disparr)
Pseudopodia
Progressive, directional
motility
Pathogenesis of E. histolytica
NONPATHOGENIC AMOEBAE
Entamoeba hartmanni
Entameoba coli
CYST
CB have splintered ends/
whisk-broom appearance
TROPHOZOITE
Largest among the amoeba
Eccentric karyosome
Eccentric karyosome
NONPATHOGENIC AMOEBAE
Endolimax nana
Iodamoeba butschlii
NONPATHOGENIC AMOEBAE
Entamoeba gingivalis
Oral cavity
With ingested WBC
No cyst form
Opportunistic Amoebae
Naegleria fowleri
Primary amoebic encephalitis (PAM)-purulent
spinal fluid without bacteria
Swimming
3 stages:
Opportunistic Amoebae
Acanthamoeba sp.
Granulomatous amoebic encephalopathy
Associated with amoebic keratitis
With spine-like projections
Acanthopodia (locomotion)
Amoebic trophozoite
Flagellate trophozoite
cyst
FLAGELLATES
Giardia lamblia
Travellers diarrhea/ Leningrads curse
Trophozoite
Pear-shaped
Falling-leaf motility
Old man with eyeglasses
4 pairs of flagella
Cyst
Retracted cytoplasm
Axonemes appear as four pairs of curved
bristles
Giardia lamblia
Giardiasis
Carpet the intestinal mucosa---duodenum and
jejunum
Steatorrhea and Jaundice
Laboratory Diagnosis:
String test
Duodenal aspirate-specimen of choice
DFS
Chiomastix mesnili
Trophozoite
Pear-shaped trophozoite
Assymetric
Spiral groove at the posterior end
Cytosome (hour-glass appearance)
Exihibits boring/cork-screw motility
Cyst
Lemon-shaped
Cytostome present
Single nucleus
Trichomonas spp.
Species:
Trichomonas vaginalis - GUT
Trichomonas tenax - oral cavity
Trichomona hominis intestines
Dientamoeba fragilis
Irregular shape with
leaf-like pseudopodia
1-2 nuclei
Karyosome is
tetracoccic/fragmented
Progressive motility
Co-infection with
Enterobius vermicularis
HEMOFLAGELLATES
Leishmania donovani
Trypanosoma gambiense
Trypanosoma rhodesiense
Trypanosoma cruzi
HUMAN
(Leishmania sp. and T. cruzi)
(Trypanosoma sp.)
TRYPOMASTIGOTE
AMASTIGOTE
INSECT
PROMASTIGOTE
Pyriform shape
Undulating membrane (1/2 of its length)
Jerky-tumbling motility
Found in urine
Trichomoniasis
Trichomonas vaginalis
EPIMASTIGOTE
Leishmania donovani
Trypanosoma gambiense
Trypanosoma rhodesiense
TRYPANOSOMES
Trypanosoma gambiense
Mid-and West African sleeping sickness
Trypanosoma rhodesiense
East african sleeping sickness
Trypanosoma cruzi
Chagas disease
Sleeping Sickness
Parasite invades the CNS
Dx:
Blood (fever), BMA, LN juice, CSF
Trypanosoma cruzi
Metacyclic trypanosomes-infective stage to
man
Reduviid bugs (Panstrongylus, Triatoma, Rhodnius)
Stages of development:
APET-man
AET-insect
Reduviid/kissing bugs
Chagas disease
Trypanosoma cruzi
Diagnosis
Romanas sign-edema of face
Demo of Amastigote in tissues
Demo of trypomastigote in blood-bulging
kinetoplast, spindle or C-shaped
Xenodiagnosis
CILIATES
Balantidium coli
Largest protozoa pathogenic to man
cecum
Trophozoite :
Cilia and cytostome
Large kidney-shaped macronucleus
Small spherical micronucleus
Cyst
Thick cyst wall, hardly stained
2 nuclei similar to trophozoite
COCCIDIA
Undergo both sexual and asexual
reproduction
Cryptosporidium parvum
Isospora belli
Sarcocystis hominis
Plasmodium falciparum
Toxoplasma gondi
Plasmodium malariae
Plasmodium ovale
Plasmodium vivax
Life Cycle
SPOROGONY
SCHIZOGONY
Gametes
combine
Trophozoite
Zygote
Schizont
Merozoite
Gametocytes
Oocyst
(with sporoblas)
Sporocyst
(with sporozoites)
Sporocyst
(into mature oocyst)
Cryptosporidium parvum
Animal parasite
One of the major opportunistic parasite in AIDS patient
(Cyclospora cayetanensis-recent)
Infects the intestine (brush border)
Oocyst has 4 sporozoites BUT NO SPOROCYST
Schizont produces 8 falciform merozoites
Diagnosis
Toxoplasma gondii
Toxoplasmosis
In adult and newborn
Resembles infectious mononucleosis, lymphadenopathies,
malaise, muscle pain, low-grade fever
Hypersensitivity
Mother to fetus transmission (tachyzoites) (transplacental
transfer and blood transfusion)
Morphology
Trophozoite pyriform of crescent shaped troph.
Tachyzoites 9acute) and bradyzoites (chronic)
Isospora belli
Human coccidiasis
Asymptomatic and self-limiting
Mature oocyst-infective stage to man
Diagnosis:
DFS
Immature oocyst in stool
Leave at room temperature
Toxoplasma gondi
Diagnosis:
Complement fixation test
Sandwich ELISA
Sabin-Feldman dye test
Frenkel skin test
PCR
Malarial Parasites
Plasmodium falciparum
Most common in the Philippines
Plasmodium vivax
Second most common the Philippines
Plasmodium malariae
Occasionally seen in the Philippines
Plasmodium ovale
Endemic in Africa, West coast, Ethiopia
G-6-PD deficiency
Sickle cell anemia trait
PABA deficiency
Recrudescence
Signa and symptoms are due to surviving parasites
in RBC
P.falciparum and P.malariae
Number of
Merozoites
in Schizont
Other
Characteristic
Normal size
Multiple
infection
Maurers dot
Small, delicate
Double chromatin
dots
Applique forms
Dark pigments
Multiple parasites
12-36
Cresent-shaped
gametocyte
Ring and
gametocyte stages
only peripheral
blood
P.malariae
72 hours
Quartan LC
Normal size
Ziemanns dots
(rare)
Compact,
May assume band
form, or rosette
6-12
Wide range of
stages in peripheral
blood
P. Vivax
48 hours
Tertian life
cycle
Enlarged
Schuffners
dots
Amoeboid
Large vacuoles
12-24
Wide range of
stages in peripheral
blood, Infects
reticulocytes
P. Ovale
48 hours
Tertian life
cycle
Enlarged
Schuffners
dots
Compact
Golden-brown
(P.vivax), double
chromatin dots
6-16
Wide range of
stages in peripheral
blood
PATHOGENESIS
The infection mainly causes tissue hypoxia,
which is due to several pathway secondary to
the presence of the parasites, mainly inside
RBC.
The ERYTHROCYTIC MEROZOITES are the
pathogenic stage of the parasite.
Trophozoite
P. falciparum
36-48 hours
Tertian life
cycle
Malarial Parasites
Resistance to malarial infection:
Red Blood
Cell
Morphology
Manner of Transmission
Babesia microti
Infects RBC
Transmitted by ticks (Ixodes spp.)
Simultaneous transmission with B. burgdorferi
Mistaken for Plasmodia
Anemia
Malaise, fever, chills
MEDICALLY IMPORTANT
PARASITES
MEDICALLY IMPORTANT
PARASITES
MULTICELLULAR
PHYLUM
Aschelminthes
SUBPHYLUM
Nematoda
(roundworms)
Platyhelminthes
Trematoda
(flukes)
CHARACTERISTICS
Adult of both sexes
Eggs, larva, adult
With of w/o intermediate
host
PHYLUM
SUBPHYLUM
Cestoda
(tapeworms)
Whip worm
Cecum
Ingestion of embryonated eggs
Adult male
Tail end is coiled 360 degrees
Female adult
Tail end is more or less straight
Egg
Foot-ball or barrel shaped with
protruding bipolar mucus plugs
CHARACTERISTICS
Ribbon-like body
Hermaphroditic
Egg,larva,adult
Mammals and insects are
intermediate hosts
Bilaterally symetric
Most are hermaphroditic
Egg,miracidium,cercaria,a
dult (EMCA)
Intermediate host (fish,
snails,crabs)
Trichuris trichiura
MULTICELLULAR
Trichinella spiralis
Trichina worm
pigs and rats are source of infection
Encysted larva-infective stage
Male and Female adult are thin and
threadlike
Male
Pair of conspicuous conical papillae
Female
Larger than male adult
Vulva about from anterior end
Encysted larva
Tightly coiled larvae between muscle
fibers enclosed by a thick capsule
C.
philippinen
sis
T. spiralis
S.
stercoralis
Hookworm
Ova
Rhabditiform Larva
Filariform Larva
Worm
Rhabditiform larva
Hookworm
S. stercoralis
Filariform larva
Sheathed
With pointed tail
With short
esophagus
Unsheathed
With notched/bifid
tail end
With longer
esophagus
N. americanus
A. duodenale
A. caninum
A. braziliense
Male N. americanus
Male A. duodenale
PARASITE
UNSHEATHED
MICROFILARIA
O.
volvulus
Nuclei do not
extend to the tip
of tail
M. ozzardi
Nuclei extend to
the tip of tail
M.
perstans
Nuclei do not
extend to the tip
of tail
MICROFILARIAE
PARASITE
SHEATHED
MICROFILARIA
W.
bancrofti
B. malayi
L. loa
Morphology of Ova
1.
2.
3.
4.
5.
P. westermani
C. sinensis
O. felineus
H. heterophyes
M. yokogawai
F. hepatica, F.
buski and E.
ilocanum
Adult Schistosoma
P. westermani
F. hepatica
F. buski
E. ilocanum
S. Japonicum
S. Mansoni
S. haematobium
C. sinensis
O. felineus
H. heterophyes
M. yokogawai
OPERCULATED EGGS
Dipyllidium caninum
1. SCOLEX/ HEAD
1.1 spatulate /almond shaped/spoon shaped and with 2
false suckers BOTHRIA
* Diphyllobothrium latum
1.2 Globular with 4 true suckers sometimes with
rostellum and hooklets
WITH HOOKS:
* T. solium
* D. caninum
* H. nana
* E. granulosus
WITHOUT HOOKS
* T. saginata
* H. diminuta
2. SEGMENTS/PROGLOTTIDS
2.1 immature
2.2 mature
* T. solium with accessory ovarian lobe
* D. caninum with bilateral genital pores
2.3 gravid/ripe
* T. solium
elongated uterus with lateral
branches ( 8-15 )
* T. saginata 15-30 branches
* D. latum
rosette shaped uterus
* E. granulosus
loosely twisted uterus
* D. caninum uterus disintegrated into Packets
* H. nana
saccular uterus
* H. diminuta Saccular uterus
1.
2.
3.
4.
5.
1.
2.
3.
4.
5.
REFRIGERATION
5% Formalin
10% Formalin
Polyvinyl Alcohol
Merthiolate Iodine
Formaldehyde
Sodium Acetate Formaldehyde
1.
2.
3.
4.
5.
6.
7.
SEDIMENTATIO
N
FLOTATION
Brines Flotation
Sheathers Flotation
1.
1.
3.
6.
7.
8.
1.
2.
3.
4.
Blood collection for Microfilaria detection should take into consideration the
PERIODICITY of the suspected filarial specie.
5.
THICK SMEAR for malaria detection is used for RAPID DIAGNOSIS and needs
to be DEHEMOGLOBINIZE, while THIN SMEAR is used for SPECIE
IDENTIFICATION and should be FIXED with alcohol
Toxoplasma gondii
Casonis test
Hydatid disease
/Echinococcus granulosus
Entamoeba histolytica
1.
2.
3.
4.
5.