9th International Symposium On Aphids (Abstract Book)

9ISA 2013
9thInternationalSymposiumonAphids
Beijing,China,26June2013
ProgramandAbstracts
InstituteofZoology,ChineseAcademyofSciences
InstituteofZoology,ChineseAcademyofSciences,Beijing,2013.06

Organizer
InstituteofZoology,
ChineseAcademyofSciences
OrganizingCommittee
GexiaQiao,President,InstituteofZoology,ChineseAcademyofSciences
XiaoleiHuang,InstituteofZoology,ChineseAcademyofSciences
LiyunJiang,InstituteofZoology,ChineseAcademyofSciences
ColinFavret,UniversityofMontreal,Canada
HongbinLiang,InstituteofZoology,ChineseAcademyofSciences
HotelLobby(1stfloor)
MeetingRooms(7thfloor)
MapGuideAroundtheHotel
1.InstituteofZoology,ChineseAcademyofSciences
2.NationalZoologicalMuseumofChina
3.BeijingOlympicParkandForestPark
4.ChinaScienceandTechnologyMuseum
5.XinaoShoppingCenter(intheOlympicPark)
YaAoHotel
BeijingSubway(OlympicGreenstationonLine8)
9thInternationalSymposiumonAphids,Beijing(China),26June2013
Program
Saturday, 1 June 2013; Hotel Lobby
8:0020:00
OnsiteRegistration(hotellobby)
18:0020:00
Dinner(1stfloor)
Sunday, 2 June 2013; 7th Floor Ballroom
8:0018:00
OnsiteRegistration(7thfloorballroom)
9:009:30
OpeningAddresses
Coordinator:XiaoleiHuang
GexiaQiaoPresidentofthe9thInternationalSymposiumonAphids
Le Kang Director of Institute of Zoology, Chinese Academy of Sciences & Director of
BeijingInstitutesofLifeScience,ChineseAcademyofSciences
ZhenyuWangDirectorofDivisionofInternationalOrganizations,BureauofInternational
Cooperation,ChineseAcademyofSciences
Session1AphidDiversityandSystematics
Chairs:ColinFavret,SamiranChakrabarti,MasakazuSano
9:30
PlenaryTalk
JeanChristopheSIMON,NOUHAUDP,JAQUIERYJ,PECCOUDJEvolutionarygenetics
and mechanisms of plant specialization and adaptive divergence in the pea aphid
complex
10:2010:30
GroupPhoto
10:3010:50
TeaBreak
10:50 QingsongZHOU,XIY,YUF,ZHANGX,LIX,LIUC,QIAOG,ZHANGYDNAbarcodingof
Hymenopteraparasitoidsrearedfromsoybeanaphid(Aphisglycines)inChina
11:10 Rui CHEN, JIANG L, LIU L, LIU Q, WEN J, ZHANG R, LI X, WANG Y, LEI F, QIAO G
ScreeninganddevelopmentofDNAbarcodinginaphids
11:30 ColinFAVRETCybertaxonomytoaccelerateaphidsystematicsandcollaboration
12:0013:40
Lunch(1stfloor)
13:40 SessionKeynoteTalk
i
DorisM.LAGOS,VOEGTLIND,COEURDACIERA,GIORDANORContributiontothe
systematicsofthegenusAphis(Hemiptera:Aphididae)
14:10 Xingyi LI, QIAO G Molecular phylogeny of the Pemphigini (Hemiptera: Aphididae:
Eriosomatinae)andevolutionofthegallmorphology
14:30 WIECZOREK K, ukasz JUNKIERT, KAJTOCH Siphini Mordvilko, 1928 (Hemiptera:
Aphidoidea:Chaitophorinae)acomprehensivereview
14:50 Jing CHEN, JIANG L, QIAO G A totalevidence phylogenetic analysis of
Hormaphidinae(Hemiptera:Aphididae)
15:1015:30
TeaBreak
BarbaraOSIADACZ,HAAJRWhyareaphidsinxerothermichabitatssounique?
16:00 Samiran CHAKRABARTI Diversity and distribution of Eriosomatinae (Aphididae:
Hemiptera) from Indian region with descriptions of two new Prociphilus Koch from
northwestHimalaya
16:20 YanFANG,CHENY,MEIL,GUOJ,LIKTheeffectofurbanizationonaphiddiversityin
differentareasofShanghai,China
16:40 Shalva BARJADZE, ASANIDZE Z, GRATIASHVILI N Morphometric investigation of the
grapevine and willow root feeding aphid, Pemphigus saliciradicis (Brner, 1950)
(Hemiptera:Aphididae:Eriosomatinae)
17:0018:00
PosterSession(7thFloorMeetingRoomC)
18:0020:00
Dinner(1stfloor)
Monday, 3 June 2013; 7th Floor Ballroom
Session2AphidsasResearchModels
Chairs:ChuncheChang,MarylnePoiri
8:30
PlenaryTalk
Christoph VORBURGER The evolutionary ecology of symbiontconferred resistance
toparasitoidsinaphids
9:20
SCHMITZ A, VILLALBA J, SIMON JC, GATTI JL, Marylne POIRI The cellular
immunity of the pea aphid model Acyrthosiphon pisum and its interactions with
symbionts
9:40
Lin LIU, Li X, Huang X, Qiao G Evolutionary relationships between Pemphigini

(Hemiptera: Aphididae: Eriosomatinae) and their primary endosymbionts Buchenra
aphidicola
ii
10:0010:20
TeaBreak
10:20 Caroline DE CLERCK, TSUCHIDA T, MASSART S, LEPOIVRE P, FRANCIS F, JIJAKLI H

Identification of bacterial symbionts in the banana aphid (Pentalonia nigronervosa)
couplinggenomicsandproteomics
10:40 ZheWANG,SUX,WENJ,JIANGL,CHENR,QIAOGWolbachiadiversityandinfection
patternsinChineseaphidpopulations
11:00 Piyaratne MKDK, ZHAO H Catastrophic behavior and aphid ecology: an analysis of
swallowtailmodelontheirpopulationdynamics
11:20 AKYILDIRIM H, zhan ENOL, GRR G, DEMRTA E The determination of the
genetic variations in Cinara cedri (Hemiptera: Aphidoidea) populations collected on
Cedrusspp.fromdifferentlocationsinTurkey
12:0013:40
Lunch(1stfloor)
Chunche CHANG, LIN Gw, LU Hl, HSIAO Ym, CHUNG Cy, WANG Sc, LIN Md
GermlinegenesandgermlinedevelopmentinthepeaaphidAcyrthosiphonpisum
14:10 SophieVANDERMOTEN,HAUBRUGEE,FRANCISFRecentadvancesonaphidalarm
pheromonebiosyntheticpathway
14:30 JosphinePIFFARETTI,VANLERBERGHEMASUTTIF,JOUSSELINEPhylogeography,life
cycleevolutionanddifferentiationofsiblingspecieswithintheleafcurlingplumaphid,
Brachycaudushelichrysi(Hemiptera:Aphididae)
14:50 YuanWANG,HUANGX,QIAOGComparativeanalysisofmitochondrialgenomesof
fiveaphidspecies(Hemiptera:Aphididae)andphylogeneticimplications
15:1015:30
TeaBreak
W.FreddyTJALLINGII,SALVADORRECATALVPhloempuncturesbyaphidstylets
16:00 Tony PRFER, THIEME T, TJALLINGII WF AutoEPG analysis of the electrically
monitoredfeedingbehaviourofaphids
16:20 JingjiangZHOUAphidOlfactionandTheirpotentialinPestmanagement
16:40 Christian C. FIGUEROA, SILVA AX, FUENTESCONTRERAS E, CARDENAS L Insecticide
resistanceoftheaphidMyzuspersicae(Sulzer)inChile
17:0018:00
18:0020:00
Dinner(1stfloor)
iii
Tuesday, 4 June 2013
LocalTour
Contact:LiyunJiang
Thisisonlyforcolleagueswhohaveregisteredthelocaltour.
Wednesday, 5 June 2013; 7th Floor Ballroom
Session3AphidBiologyandEcology
Chairs:ChrisShortall,FengGe
8:30
PlenaryTalk
LUY,KongmingWU,DESNEUXNBtcottonadoptionpromotesbiocontrolservicesin
agriculturallandscape
9:20
AbidALI,DESNEUXN,LUY,WUKEffectsofdifferentnaturalenemiesonpopulation
dynamicsofwheataphids
9:40
AMEIXAOMCC,MESSELINKGJ,PavelKINDLMANNNonlinearitiesleadtoqualitative
differencesinpopulationdynamicsofpredatoraphidsystems
10:0010:20
TeaBreak
10:20 ChengQU,LIUA,XUEMTheinterspecificcompetitionofBemisiatabaciBbiotype
andMyzuspersicaemediatedbycabbage
10:40 Claudio C. RAMREZ, ORTZ S, LAVANDERO B Defensive behavior of aphids against
parasitoidsexhibitinghostfidelity
11:00 ukasz DEPA, MRZ E Polyphagism of Stomaphis wojciechowskii Depa (Aphididae,
Lachninae)ecologicalimportanceandsignificanceforspeciesrecognition
11:20 HuanhuanGAO,ZHAOH,DUC,DENGM,DUE,HUZ,HUXLifetableevaluationof
survival and reproduction of the aphid, Sitobion avenae (Hemiptera: Aphididae),
exposedtocadmium
12:0013:40
Lunch(1stfloor)
Maurice HULL Myzus ascalonicus in the subAntarctic: a story of a biological
invasion
14:10 MohsenMEHRPARVAR,WEISSERWWMetacommunityecologyofspecializedtansy
aphids
14:30 RimantasRAKAUSKAS,ZAREMBAAPlum(Prunusspp.)aphidguildstructureinthe
EasternBalticregionofEurope
iv
14:50 Xiangshun HU, LIU X, HU Z, ZHAO H, LIU T Correlations among growth and
reproductionparametersforthreecerealaphidsontenwheatlines
15:1015:30
TeaBreak
Yucheng SUN, GUO H, GE F Pea aphid promotes amino acid metabolism both in
Medicago truncatula and bacteriocytes to favor aphid population growth under
elevatedCO2
16:00 Liping BAN, SUN Y, ZHANG S Immunolocalization of odorantbinding proteins on
antennalchemosensillaofthepeachaphidMyzuspersicae(Sulzer)
16:20 ZuqingHU,ZHAOH,THIEMETTheeffectsofenhancedUltravioletBradiationonthe
biologyofgreenandbrownmorphsofSitobionavenae(Hemiptera:Aphididae)
16:40 JieYANG,ZHANGL,GAOH,ZHOUD,ZHAOHTheeffectsofincreasedUVBradiation
onsurvival,development,andgeneexpressioninSitobionavenae
17:0018:00
18:0020:00
Dinner(1stfloor)
Thursday,6June2013;7thFloorBallroom
Session4AphidsinAgriculture,HorticultureandForestry
Chairs:TongxianLiu,JingjiangZhou
8:30
PlenaryTalk
Karam B. SINGH, KAMPHUIS LG, ZULAK K, GUO S, LICHTENSVEIG J, EDWARDS O,
ANDERSONJ,GAOLResistancetoaphidsinamodelplant,MedicagoTruncatula
9:20
YUX,FANJ,ZHANGM,WANGH,HEY,WANGG,MAY,CHENJ,ZHANGY,LanqinXIA
Generationofanoveltypeoftransgenicwheatemittingaphidalarmpheromonefor
aphidcontrol
9:40
Sophie THOMAS, VANLERBERGHEMASUTTI F, BOISSOT N Effects of genetic

resistance combinations in melon on density and genetic diversity of Aphis gossypii
populationsinfields
10:0010:20
TeaBreak
10:20 HuiyanZHAO,THIEMET,HEIMBACHU,HUZ,HUX,LUOK,WANGCThestudieson
thecropresistancetoaphid
10:40 Arnaud AMELINE, POINTEAU S, JAGUENET E, RAMBAUD C, COUTY A Resistance of
the bioenergy crop miscanthus towards the corn leaf aphid, Rhopalosiphum maidis
v
(Fitch)(Hemiptera:Aphididae)
11:00 Deguang LIU, Huang X Lifehistory trait differentiation of Sitobion avenae
populationsfrombothsidesoftheQinlingMountains
11:20 SuxiaGAO,HUANGX,LIUDComparisonofSitobionavenaelifehistoriesonoatand
barley
12:0013:40
Lunch(1stfloor)
MarilyneUZEST,GARGANID,THILLIERM,PIROLLESE,BLANCSCharacterisationof
the Acrostyle, an organ present at the tip of aphid maxillary stylets involved in
noncirculativevirustransmission
14:10 Sbastien BOQUEL, ALDAOUD F, ZHANG J, FAGERIA M, GIGUERE MA, CLARK C,
PELLETIERYNewinsightsinmineraloileffectsonAphidPotatoPVYpathosystem
14:30 ChrisSHORTALL,BELLJ,FOSTERS,WILLIAMSONM,BRUCET,HARRINGTONRGoing
againstthegrainaphid
14:50 Zhaozhi LU, ZALUCKI MP, PERKINS LE, WANG P, GAO G The spatialtemporal
dynamics of Aphis gossypii at a regional scale in Northwestern China: developing
forecastsformanagementofthispest
15:1015:30
TeaBreak
15:30 RegistrationAwardWinnerTalk
Mitzy PORRAS, RAJOTTE E, CACIAGLI P, TOOKER J, ROSA C, RUSSO J, GUGINO B,
GILDOWFEThedevelopmentofasmartphonesupportplatformformonitoringand
managementofaphidsbarleyyellowdwarfvirusonsmallgrainsinPennsylvania
16:00 RolandSIGVALDForecastingpotatovirusesinseedpotatoesusingsuctiontraps
16:20 HongbinLIANG,QINQ,QIAOGSuctiontrapsformonitoringaphidsinChina
16:40 Mingzhen PAN, LIU T Adaptation and fitness of Aphidius gifuensis (Hymenoptera:
Braconidae)ondifferentaphidspecies
17:0018:00
ClosingSession
Coordinator:ColinFavret
ZhiyiLiDeputyDirectorofInstituteofZoology,ChineseAcademyofSciences
18:0020:00
vi
FarewellParty(9thFloorSunnyHall)
ListofPosters
S1.1
Shalva BARJADZE, BLACKMAN RL, BARBAGALLO S Silenefeeding aphids of the

MacrosiphumhartigigroupinsouthernEurope
S1.2
RuiCHEN,QIAOGAnewmethodofDNAbarcodinginaphids
S1.3
ENOL , AKYILDIRIM H, Emin DEMRTA, GRR G Determination of the

morphometric variations in Hyalopterus pruni (Hemiptera: Aphidoidea) populations
associatedwithdifferenthostplantsandlocalities
S1.4
WIECZOREKK,KAJTOCH,ukaszJUNKIERTChaitophorinaeversusDrepanosiphinae
(Hemiptera:Aphidoidea)preliminarydataofmolecularanalysis
S1.5
QinghuaLIU,JIANGL,QIAOGDNAbarcodingofGreenideinae(Hemiptera:Aphididae)
withresolvingtaxonomyproblems
S1.6
EwaMRZ,TRELAJ,DEPAMolecularandmorphologicaldifferentiationofLachnus
pallipes(Hrtig)andL.roboris(L.)(Aphididae,Lachninae)
S1.7
AKYILDIRIMH,OLCABEYG,zhanENOL,GRRGThedeterminationofthegenetic
diversity of Pemphigus spyrothecae (Hemiptera: Aphidoidea) distributed in Istanbul,
NideandKayseriProvincesofTurkey
S1.8
Xiaomin SU, WANG Z, JIANG L, QIAO G The population genetic structure of Myzus
persicaeinChina
S1.9
JurgaTURCINAVICIENE,RAKAUSKASROnthemolecularsystematicsofgenerawithin
tribeAphidini(Hemiptera,Aphididae)
S1.10
JuanWEN,CHENR,JIANGL,QIAOGRapididentificationofaphidsonflowerplantsof
RosaceaeinBeijingbasedonDNAbarcoding
S2.1
Lingling GAO, KAMPHUIS LG, EDWARDS O, SINGH KB Multiple mechanisms may be

involvedintheresistanceofMedicagotruncatulatoaphidTherioaphistrifolii
S2.2
ANSELME C, COLINET D, DELEURY E, MANCINI D, TARES S, POULAIN J, BELGHAZI M,

PENNACCHIOF,POIRIM,JeanLucGATTIVenomtranscriptomicsandproteomicsof
Aphidiuservi,parasitoidoftheaphidmodelAcyrthosiphonpisum
S2.3
YiminHSIAO,CHANGCcDppsignalinginthedevelopmentoftheasexualpeaaphid
S2.4
Barbara OSIADACZ, HAAJ R, WRZESISKA I Bioindication value of Pemphigus

spyrothecaePasserini,1856(Hemiptera,Aphidoidea,Eriosomatidae)
vii
S3.1
Jingjiang ZHOU, VIEIRA FG, HE X, SMADJA C, LIU R, ROZAS J, FIELD LM The

odorantbindingproteingenefamilyinthepeaaphid,Acyrthosiphonpisum
S3.2
BOSQUE E, Sophie VANDERMOTEN, YU W, YIN R, LIU Y, CHEN J, FRANCIS F

Identification of lectins as virus competitors in aphid vectors: from fundamental to
appliedapproaches
S3.3
JiaFAN,CHENJ,YUANH,CHENGD,SUNJ,LIUYIdentificationofintraspeciesalarm
pheromone and two conserved odorant binding proteins associated with
EFarneseneperceptioninRhopalosiphumpadi(Linnaeus)
S3.4
XiangdongLIU,WUW,LIANGX,ZHAOH,XUTHostplantdeterminesthehostrange
ofcottonmelonaphidandthetacticsoftheaphidtoquicklyadaptanovelhost
S3.5
ZhaohuanXU,WANGJ,LUOM,YUMPopulationdynamicsofMyzuspersicaeandits
naturalenemiesinthetobaccofieldsofJiangxi,China
S3.6
Wenjuan YU, XU Z, CHEN J, BRAGARD C, FRANCIS F, LIU Y, CHENG D Variation in

transmission of Barley yellow dwarf virusPAV by different populations of Sitobion
avenaeinChina
S3.7
XiaoningZHANG,CHENJ,CHENGD,SUNJAphicidalactivityofmajoralkaloidsfrom
Macleayacordataextractagainstwheataphids
S3.8
XingyaWANG,ZHOUL,XINGX,XUB,XUGPopulationdynamicsanddemographyof
thesoybeanaphid(Aphisglycines)andphysiologicalresponsesofthesoybeanplants
S4.1
DEBBABIM,ATTOUMBRJ,BALTORAS,ArnaudAMELINEDirectandindirecteffects
ofglycoalcaloidsonaphids
S4.2
PHILIPPI J, Edgar SCHLIEPHAKE, JRGENS HU, JANSEN G, ORDON F, KAUFMANN K

RelationsbetweenaphiddevelopmentandalkaloidcontentofLupinusangustifolius
S4.3
YongZHANG,CHENJ,FANJ,CHENGD,SUNJcDNAcloningofC002,asalivarygland
proteiningreenbug,Schizaphisgraminum
S4.4
Alexandra SCHOENY, GOGNALONS P, BOISSOT N, MISTRAL P, CHAREYRON V,

WIPFSCHEIBEL C, LECOQ H Combination of genetic resistance and management of
fieldborders:towardsaneffectiveandsustainablecontrolofAphisgossypiioutbreaks
andvirusepidemicsincantaloupemeloncrops
S4.5
RonglingYIN,FRANCISF,BRAGARDC,LIUY,CHENJStudyontransmissionefficiency
ofCMVbyMyzuspersicaefromdifferentplaces
S4.6
FLETCHERJ,MelanieDAVIDSON,HORROCKSA,SKILLSYDVstrainsinautumnsown
wheatinCanterbury,NewZealand
S4.7
Melanie DAVIDSON, LOGAN D, HOWLETT B, SKILL S, READ S, HILL G Landscape

ecologyofaphidsandgeneralistpredatorsinanarableandforagecropsenvironment
viii
S4.8
JunheLIU,YUM,ZHAOZDensityestimationofgrounddwellingpredatorsinwheat
fieldsinNorthwestChina
S4.9
Zihua ZHAO, HUI C, ZHAO Y, AN R, HE D Effects of withinfield position and

surrounding habitats on the community of the natural enemies of cereal aphids in
wheatfield
S4.10
Hanglian GONG, GONG C, LI M Rice aphid diversity, occurrence and prevention in

Pingxiang
S4.11
Fangmei ZHANG, LI X, ZHANG Y, CHENG D Studies on the external morphology

comparisonsbetweenwingedandwinglessofSitobionavenae(Fabricius)(Hemiptera:
Aphididae)
S4.12
SbastienBOQUEL,GIGUEREMA,PELLETIERY,PRIVEJPMineraloilpenetrationand
translocationinpotatoplant
S4.13
CongWEI,WANGH,HEHMutualisticinteractionbetweenantsandaphidsthrough
thehoneydew:bacterialcompositionanddiversityinthehoneydewofCinaratujafilina
(delGuercio)
S4.14
Qilian QIN, LIANG H, MIAO L, ZHANG H, QIAO G Development and application of

suctiontrap,aninstrumenttomonitortheoccurrenceofaphidsandothermigratory
insects
ix
OralAbstracts
PlenaryTalk
Evolutionary genetics and mechanisms of plant specialization and

adaptive divergence in the pea aphid complex
JeanChristopheSIMON,PierreNOUHAUD,JulieJAQUIERY,JeanPECCOUD
INRA,UMR1349,InstitutdeGntique,EnvironnementetProtectiondesPlantes,DomainedelaMotte35653Le
RheuCedex,France.(jeanchristophe.simon@rennes.inra.fr)
Theroleofecologicalfactorsandbiologicaladaptationsonspeciesformationhasgainedstrongsupportin
thelastdecade.However,theprecisestepsinvolvedintheaccomplishmentofecologicalspecializationand
reproductive isolation are not fully understood, neither is the evolutionary time needed for such an
accomplishment.Inaddition,thegenomicarchitectureunderlyingadaptivetraitsispoorlyknown,especially
inthecaseofreplicatedeventsofadaptivedivergence.Here,Iwillpresenttherecentprogresswemadeon
these issues, taking advantage of the pea aphid species/biotype complex, which conveniently shows a
continuum of genetic divergence from sympatric host races to incipient species. By deeply exploring the
evolutionary genetics of plant adaptation and ecological speciation using a multidisciplinary approach, we
showed in particular that the pea aphid complex underwent a recent and rapid ecological diversification
throughmultiplehostshifts.Wealsoidentifiedgenomicregionsputativelyinvolvedinhostplantadaptation
by performing a genome scan analysis with high throughput genotyping technologies. Interestingly, these
genomic regions include chemosensory or salivary genes, both of which being possible gene candidates
responsibleforplantspecializationinthepeaaphidcomplex.
Keywords:ecologicalspeciation;candidategenes;adaptiveradiation.
DNA barcoding of Hymenoptera parasitoids reared from soybean

aphid (Aphis glycines) in China
QingsongZHOU1,2,YuqiangXI1,FangYU1,XuZHANG1,XuejunLI3,ChunlaiLIU4,GexiaQIAO1,
YanzhouZHANG1
1
Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences,
ChaoyangDistrict,Beijing100101,China;(zhangyz@ioz.ac.cn)
2
SchoolofLifeScience,AnhuiUniversity,Hefei230039,China;
3
CollegeofChemistryandLifeSciences,ShenyangNormalUniversity,Shenyang110034,China;
4
PlantProtectionInstitute,HeilongjiangAcademyofAgriculturalSciences,Harbin150086,China.
DNA barcoding is a useful method to help species identification and has been used to study biodiversity,
cryptic species and food webs, especially for insects, the most diverse groups of animals on the planet.
Amongthem,afewcasestudiesaboutparasiticwaspsindicatedDNAbarcodeshasapotentialidentification
power for the parasitoids of economic important insect pests. To verify whether DNA barcoding is an
effective means for soybean aphid parasitoid identification, 69 specimens (including primary and
hyperparasitoids) reared from soybeanaphid, collected from Henan, Heilongjiang, Liaoning, Anhui, Yunnan
provinces and Beijing and Chongqing during 20092012, were selected to represent each species and
barcoded.Twogenes(28SD2andCOI)wereamplifiedandsequencedasbarcodesofthespecimens.DNA
barcodinganalysisbaseonintrainterspeciesdistancesandphylogeneticanalysisusingNJtreeindicatethat
thereare15species(6primaryand7hyperparasitoids),including6primaryparasitoids:Lysiphlebusfabarum,
L. orientalis, Binodoxys communis, Aphidius uzbekistanicus, Pseudopraon sp. (Aphidiidae); Aphelinus
albipodus (Aphelinidae), and 9 hyperparasitoids: Syrphophagus aphidivorus, Syrphophagus sp. (Encyrtidae);
3
Pachyneuronaphidis,Asaphessuspensus,Anisopteromalussp.(Pteromalidae);MariettapictaandAphelinus
sp. (Aphelinidae); Alloxysta chinensis and Alloxysta sp. (Figitidae). The present study demonstracted that
soybean aphid parasitoids can be efficiently identified through the use of DNA barcoding, even for the
species complex of closely related species, and that the present 28SD2 and COI library can be used for
subsequentapplicationsinpestbiologymanagement.
Keywords:DNAbarcode;taxonomy;COI;28S.
Screening and development of DNA barcoding in aphids
RuiCHEN1,2,LiyunJIANG1,LinLIU1,2,QinghuaLIU1,2,JuanWEN1,2,RuilingZHANG1,2,XingyiLI1,2,
YuanWANG1,2,FuminLEI1,GexiaQIAO1
KeyLaboratoryofZoologicalSystematicsandEvolution,InstituteofZoology,ChineseAcademyofSciences,No.1
BeichenWestRoad,ChaoyangDistrict,Beijing100101,China;(chrui11@live.cn)
2
UniversityofChineseAcademyofSciences,No.19,YuquanRoad,ShijingshanDistrict,Beijing100049,China.
DNAbarcodingusesastandardDNAsequencetofacilitatespeciesidentification.AlthoughtheCOIgenehas
beenadoptedasthestandard,COIaloneisimperfectduetoshortcomings.ToevaluatetheutilityofCOIasa
DNA barcode and other barcode genes, and to complement drawbacks of the COI locus, the results of
experimentalresearchbasedontaxonomywereusedtostudyDNAbarcodinginaphids.Wefirstchoseover
80speciesoftheaphidsubfamilyLachninae(Hemiptera:Aphididae)asthefocusofourstudyandcompared
theresults obtained usingCOI withtwoother mitochondrial genes, COII and Cytb. The COI and COII genes
demonstrated a greater PCR amplification efficiency than Cytb. Species identification using COII sequences
hadahigherfrequencyofsuccess(96.9%inbestmatchand90.8%inbestclosematch)andyieldedlower
intra and higher interspecific genetic divergence values than the other two markers. The Cytb gene is an
effectivemarkerforthestudyofaphidpopulationgeneticsduetoitshighsequencediversity.ACOIIbased
identificationsystemshouldbemoreeffectiveinidentifyinglachninespeciesthanCOIorCytb.Inaddition,
Buchnera,theprimaryendosymbiontofaphids,hasahigherevolutionaryrateandinterspecificdivergence
thanitscodivergingaphidhosts,makingitapotentialtoolforresolvingtheambiguitiesinaphidtaxonomy.
WecomparedtheeffectivenessofemployingtwodifferentDNAregions,BuchneragndandmitochondrialCOI,
forthediscriminationofover100speciesofaphids.Themeaninterspecificdivergenceofthegndregionwas
significantly higher than the mean intraspecific variation; there were nearly nonoverlapping distributions
between the intra and interspecific samples. In contrast, COI showed a lower interspecific divergence,
whichledtodifficultiesinidentifyingcloselyrelatedspecies.Ourresultsshowthatgndcanidentifyspeciesin
the Aphididae, which suggests that the gnd region of Buchnera is a potentially effective barcode for aphid
speciesidentification.Wealsorecommendthe2locuscombinationofgnd+COIastheaphidbarcode.This
2locus barcode will provide a universal framework for the routine use of DNA sequence data to identify
specimensandcontributetowardthediscoveryofoverlookedspeciesofaphids.
Keywords:COI;Lachninae;COII;Buchnera;gnd.
Cybertaxonomy to accelerate aphid systematics and collaboration

ColinFAVRET
University of Montreal, Biodiversity Centre, 4101 rue Sherbrooke est, Montreal, Quebec H1X 2B2, Canada.
(ColinFavret@AphidNet.org)
Almostallaphidtaxonomicworkhasbeenrestrictedtoparticularregionsoftheworld.Comprehensiveglobal
revisions generally treat only small groups and larger groups tend to accumulate new taxa without being
treatedasawhole.Therearealmost5,000validaphidspeciesandover500validaphidgenera.Onefifthof
thespeciesbelongtooneofthreegenera(Aphis,Cinara,Uroleucon)andhalfofthegenerabelongtoonly
4
onesubfamily(Aphidinae,thevastmajorityinMacrosiphini).Aglobalrevisionofanyoftheselargestoftaxa
has been practically unfeasible. Until now! I will present novel systematics tools and methods that will
facilitate a level of collaboration never before possible: 1) high resolution virtual specimens to examine,
measure, and identify online; 2) specimen databases linking taxonomic, locality, and host data from
collections around the world; 3) database modules to autogenerate significant portions of manuscripts,
autopopulateonlineinteractiveidentificationkeys,andexportdatainanunlimitedvarietyofconfigurations.
Iwillpresentsmallerpilotprojectsatornearingcompletionandproposeambitiouscollaborativeendeavors.
Keywords:virtualspecimen;virtualcollection;database;interactiveidentificationkey;microscopy.
Session Keynote Talk
Contribution to the systematics of the genus Aphis (Hemiptera:

Aphididae)
DorisM.LAGOS1,DavidVOEGTLIN2,ArmelleCOEURDACIER3,RosannaGIORDANO2
Department of Entomology, University of Illinois at UrbanaChampaign, Illinois, Urbana 61801, USA;

(dlagos@illinois.edu)
2
IllinoisNaturalHistorySurvey,PrairieResearchInstitute,Illinois,Champaign61820,USA;
3
Centre de Biologie pour la Gestion des Populations, Campus International de Baillarguet CS 30016, 34988
MontferriersurLezcedex,France.
AphylogenyofthegenusAphisLinnaeus,1758wasbuiltprimarilyfromspecimenscollectedintheMidwest
of the USA. A topology using UPGMA was constructed with 73 taxa and 41 morphological characters with
characterstatesofalateandapterousviviparousfemales.Weidentifiedfourmainspeciesgroups:gossypii,
asclepiadis, middletonii, and fabae. Dendrogram topologies of analyses performed using Maximum
ParsimonyandBayesiananalysisofcytochromeoxidaseI(COI),nuclearelongationfactor(EF1)andprimary
endosymbiontBuchnera16S(Buch16S)sequenceswerenotcongruent.Bayesiananalysisstronglysupported
most of the terminal nodes of the phylogenetic trees. The phylogeny was strongly supported by detailed
morphological characterization, EF1 and combined sequences of COI and EF1 with morphological
characters. It was not supported by single analysis of COI or Buch 16S. Our results showed that Toxoptera
Koch, 1856 should be treated as a subgenus of Aphis. The current subgenus Bursaphis Baker, 1934 is a
synonymofAphiss.str.ThephylogenydemonstratedtheNorthAmericanspeciesofthemiddletoniigroup
belongtoXerobionNevsky,1928.ThecurrentgenusXerobionisaseniorsynonymofProtaphisBrner,1952.
ThegenusIowanaHottes,1954isajuniorsynonymofAphiss.str.
Keywords:aphid;hostplant;morphology;phylogeneticrelationship;sequencedivergences;synonym.
Molecular phylogeny of the Pemphigini (Hemiptera: Aphididae:

Eriosomatinae) and evolution of the gall morphology
XingyiLI1,2,GexiaQIAO1
1
ChaoyangDistrict,Beijing100101,China;(idean1982@hotmail.com)
2
UniversityofChineseAcademyofSciences,ShijingshanDistrict,Beijing100049,China.
Theevolutionofgallsisaseriesofadaptationsbetweenthehostplantsandtheinducinginsectsinahistorical
context.AphidsofthetribePemphiginitypicallyinducegallsontheirprimaryhosts,andthegallmorphology
differsamongspecies.PreviousstudieshavediscussedtheevolutionofgallpositionsinPemphiginibasedon
molecular phylogenetic evidence from a single gene dataset, but few species were sampled and the
phylogeneticresolutionwasweak.WesampledadditionalspeciesinPemphiginirepresentingmosttypesof
5
galls, and analyzed the molecular phylogeny with parsimony, maximum likelihood and Bayesian methods,
combiningsequencesfromthemitochondrialgenesCOIandCOIIandthenucleargenesEF1andLWO.The
ancestralstatesofgallmorphology,includingposition,sizeandshape,werereconstructedonthephylogeny
afterwards. Results show that Pemphigini is monophyletic but the genus Pemphigus is not. The gall
morphologyshowsamajorevolutionaryroutefrompseudogalltosubstantialgall,fromtheleafbladetothe
petioleandthetwig,andfromglobulartocomplicatedshapeswiththesizetendingtoincrease.Themajor
route is roughly consistent with previous studies, but the overall pattern is not as linear because of the
presenceofbranchingevents.Besides,thepreferenceofsmallglobulargallslocatingonthejointoftheleaf
andpetioleisindicatedinPemphigus.Thepreferenceofthegallmorphologyandtheevolutionarytendency
couldbeexplainedbyatradeoffofhabitatselectionoftheaphidsandthenutritionhypothesis.
Keywords:gallshape;gallposition;habitatselection;monophyly;Pemphigus;thenutritionhypothesis.
Siphini Mordvilko, 1928 (Hemiptera: Aphidoidea: Chaitophorinae) a

comprehensive review
KarinaWIECZOREK1,ukaszJUNKIERT1,ukaszKAJTOCH2
UniversityofSilesia,DepartmentofZoology,Bankowa9,40007Katowice,Poland;(lukasz.junkiert@wp.pl)
InstituteofSystematicsandEvolutionofAnimals,PolishAcademyofScienceDepartmentofExperimentalZoology,
Sawkowska17St.,31016Krakow,Poland.
TheaimofthestudywastogenerateacomprehensivephylogenyfortheSiphiniMordvilko,1928(Hemiptera:
Aphidoidea: Chaitophorinae) on the basis of a diversity of methods and data sets. Reconstructions were
basedonmorphologicalandecologicalcharactersaswellasmoleculardatanucleargeneselongationfactor
1 (EF1) and 18S rDNA, and mitochondrial genes cytochrome oxidase I and II (COI and COII) and
nicotinamide adenine dinucleotide dehydrogenase 1 (ND1). Moreover, the structure of the reproductive
system of adult males of selected species of Siphini has been studied under light and transmission and
scanningelectronmicroscopy.Allavailabledataconcerningchorology,ecologyandbionomyofthespecies
belonging to this tribe was summarized and the origin of this group of aphids was proposed. Aphids
belongingtoSiphinirepresentagroupofinsectsassociatedwithgrasses(Poaceae)andsedges(Cyperacea)
andareregardedastheyoungestbranchofChaitophorinae.Chaitophorini,thesecondtribeofthissubfamily,
are connected with deciduous trees the genera Chaitophorus Koch, Chaitogenophorus Zhang, Qiao and
Chen, Lambersaphis Narzikulov, and Pseudopterocomma MacGillivray with Salicaceae whereas the genera
PeriphyllusvanderHoeven,TrichaitophorusTakahashiandYamatochaitophorusHiguchiwithAceraceae.In
phylogenetictrees,reconstructedonthebasisofmorphologicalandmoleculardatasetsthemonophylyof
Siphiniwasconfirmed.However,preliminarydatashowthattheChaitophorinidonotformamonophyletic
clade.ThegenusPeriphyllusformsanindependentlineagefromsistercladesChaitophorusandSiphini.
2
Keywords:aphid;distribution;hostplants;moleculardata;morphology;phylogeneticrelationship.
ThisresearchwassupportedbytheMinistryofScienceandHigherEducation,Poland,grantnoNN303392636.
A total-evidence phylogenetic analysis of Hormaphidinae (Hemiptera:

Aphididae)
JingCHEN1,2,LiyunJIANG1,GexiaQIAO1
ChaoyangDistrict,Beijing100101,China;(chenjing@ioz.ac.cn)
2
CollegeofLifeSciences,UniversityofChineseAcademyofSciences,ShijingshanDistrict,Beijing100049,China.
AphylogeneticanalysisofHormaphidinaeispresentedbasedonatotalevidenceapproach.Fourgenes(two
6
mitochondrial, COI and CytB, and two nuclear, EF1 and LWO) are combined with 65 morphological and
sevenbiologicalcharacters.Sixtythreehormaphidinespeciesrepresentingthreetribesand36generaaswell
asnineoutgroupsareincluded.Parsimonyandmodelbasedapproachesareused,andseveralsupportvalues
andimpliedweightingschemesareexploredtoassesscladestability.ThemonophylyofHormaphidinaeand
Nipponaphidiniissupported,butCerataphidiniandHormaphidiniarenotrecoveredasmonophyletic.Based
on the parsimony hypothesis from the totalevidence analysis, the phylogenetic relationships within
Hormaphidinae are discussed. Cerataphidini is redelimited to exclude Doraphis and Tsugaphis, and
HormaphidiniisredefinedtoincludeDoraphis.CeratocallisQiao&Zhangisestablishedasajuniorsynonymof
CeratoglyphinavanderGoot,syn.nov.LithoaphisquercisuctaQiao,Guo&Zhangistransferredtothegenus
NeohormaphisNoordamasNeohormaphisquercisucta(Qiao,Guo&Zhang)comb.nov.
Keywords:aphid;hormaphidine;phylogeny;combinedanalysis.
Why are aphids in xerothermic habitats so unique?
BarbaraOSIADACZ1,RomanHAAJ2
DepartmentofEntomologyandEnvironmentalProtection,PoznaUniversityofLifeSciences,Dbrowskiego159,
60594Pozna,Poland;(barbara.osiadacz@gmail.com)
2
TheUpperSilesianNatureSociety,Huberta35,40543Katowice,Poland.
Xerothermichabitatsarecharacterisedbywarmanddryconditions,ahighplantdiversity,andconsequently
an abundant invertebrate fauna, particularly insects. Although in southern Europe they are still quite
abundant,xerothermichabitatsaredeclininginotherregions.Withaviewtopreservingearthsbiodiversity,
xerothermichabitatswerespecificallymentionedintheCouncilDirective92/43/EECof21May1992onthe
conservation of natural habitats and of wild fauna and flora (the habitat directive in EU law), which
constitutesabasisforaEuropeanconservationprogrammecalledNature2000.Outof1,500aphidspecies
recordedinEurope30%canbefoundinxerothermichabitats,definedbroadly.ThemajorityareAphididae
species(e.g.Aphis,Brachycaudus,Dysaphis,Macrosiphoniella,Protaphis,Uroleucon),fewerofthembelong
to the Drepanosiphidae (especially Siphini), Eriosomatidae (Fordini) and the fewest to Anoeciidae. Some
species occur frequently both in xerothermic and replacement, i.e. anthropogenic habitats (e.g. Aphis
euphorbiae, Macrosiphoniella millefolii, Uroleucon jaceae), and there are others whose occurrence is
restrictedexclusivelytonaturalxerothemichabitats.Severalyearsofstudieswithstandardisedqualityand
quantity methods helped to recognise such species (e.g. Aphis thesii, A. teucrii, Brachycaudus salicinae,
Uroleuconensifoliae)andrecordsomespeciespreviouslyunknown(Acaudinumsp.,Anoeciasp.).Therarity
of some species may be a result of their biology, endangered ecosystem (subject to humandevelopment
pressure) or other significant criteria. Determining the most valuable regions is of utmost importance to
protectearthsbiodiversity.
Keywords: Aphidoidea; biodiversity; nature conservation; rare and endangered species; taxonomy;
xerothermalgrasslands.
Diversity and distribution of Eriosomatinae (Aphididae: Hemiptera)

from Indian region with descriptions of two new Prociphilus Koch from
northwest Himalaya
SamiranCHAKRABARTI
Biosystematics Research Unit, Department of Zoology, University of Kalyni, Kalyani 741235, India.
(chakrabarti32b@gmail.com)
Sofar,64speciesoferiosomatineaphidsareknownfromtheIndianregion(Ghosh,1984;Chakrabarti,2006,
2009). However, during extensive surveys, three species of Pemphigus Hartig (i.e. P. indicus Keifer, P.
nainitalensis Chlodkovsky and P. spirothecae Passerini) described/reported from northwest Himalaya and
CeratopemphigelladelhiensisMenonandPowerdescribedfromDelhi(Indusvalley)couldnotbeencollected
further.Subsequentreferencestothesespeciesfromtheregionrefertothefirstreportofthesespeciesonly.
Threespecies,i.e.GharesiapoluniniStroyan,ProciphilusghaniiStroyanandTetraneurapolychaetaHilleRis
Lambers,arestillrestrictedtotheHimalayanpartinPakistanwhileCeratopemphiguszehntneriSchouteden
andKaltenbachiellajaponica(Matsumura)havebeenreportedonlyfromSriLanka.Threetaxaarereported
uptogenericlevelsonly.ReportsoffewProciphilusspeciesfromthisareaseemtobedoubtful.Manyspecies
arereportedonlyontheirsecondaryhosts.Inthispaperthestatusoferisomatineaphidspeciesreportedso
farfromtheIndianregionisdiscussed.TwonewspeciesofProciphilusKochareaddedtothislist,described
and their relationships with other species of the genus are also discussed. Morphology of galls induced by
severalspeciesisdescribed.Besides,distributionandhostassociationofthesespeciesarediscussed.
Keywords:eriosomatineaphids;hostassociation;gallmorphology;newspecies.
The effect of urbanization on aphid diversity in different areas of

Shanghai, China
YanFANG1,2,YingCHEN1,2,LuMEI1,JiangliGUO1,KaiLI1,2
1
2
SchoolofLifeScience,EastChinaNormalUniversity,Shanghai200062,China;(yfang@bio.ecnu.edu.cn)
ShanghaiKeyLaboratoryforEcologyofUrbanizationandEcologicalRestoration,Shanghai200062,China.
Urbanization refers to the process by which rural areas become urbanized as a result of economic
developmentandindustrialization.Thisprocessmayhaveaseriousimpactonthelivingthings,especiallythe
largest such group insects. Their diversity may show a conspicuous variety among areas with different
levelsofurbanization.Aphidsaregoodcandidatestostudytheeffectofurbanization.Anaphidinvestigation
wasconductedinthreedifferenturbanizedhabitats,i.e.urbancenter,suburbanandruralarea,duringApril,
May, and June from 2010 to 2011 in Shanghai. A total of 350 specimens were collected, belonging to 61
species,38generaand6families.Theresultsshowedthatthenumberofspecieswashighestinthesuburban
area (43 species from 152 specimens) and lowest in the urban center (29 species from 85 specimens).
Diversityindexeswereusedtoanalyzethespeciesandcommunitystructureofaphidsinthesethreeregions.
Itwasshowedthatthediversityindexofsuburbanareawasthehighest(ShannonWeinnerdiversityindex:
3.270, Margalef species abundance index: 8.367), while the urban center area was the lowest
(ShannonWeinnerdiversityindex:3.000,Margalefspeciesabundanceindex:6.306).Alongthecityhabitat
gradient,fromurbancentertoruralareaswithhumaninterferencehighertolower,aphiddiversityfollowed
thetrendoffirstincreasing,thendeclining.Similarityanalysesshowedthatthesimilaritycoefficientofthe
suburban and rural areas was the highest (0.625), while the similarity coefficient of the urban center and
ruralareawasthelowest(0.515).Differentlevelsofurbanizationhavesignificanteffectonaphiddiversity;
aphiddiversitycanbeusedasareferenceindexofShanghaiurbanization.
Keywords:insectdiversity;diversityindex;urbanecology;humaninterference.
Morphometric investigation of the grapevine and willow root feeding

aphid, Pemphigus saliciradicis (Brner, 1950) (Hemiptera: Aphididae:
Eriosomatinae)
ShalvaBARJADZE1,ZezvaASANIDZE2,NanaGRATIASHVILI3
EntomologyandBiocontrolResearchCentre,AgriculturalUniversityofGeorgia,13thkmofDavidAghmashenebeli
Alley,Tbilisi0131,Georgia;(shalva.barjadze@yahoo.com)
8
2
3
InstituteofEcology,IliaStateUniversity,CholokashviliAve.3/5,Tbilisi0162,Georgia;
InstituteofZoology,IliaStateUniversity,GiorgiBrtskinvalestr.5,Tbilisi0162,Georgia.
Pemphigus saliciradicis (Hemiptera: Aphididae: Eriosomatinae) lives on roots of Salix spp. (Salicaceae) and
Vitis vinifera (Vitaceae) in the Holarctic region. Morphometric data for eight morphological characters,
measured from 10 field collected samples (50 specimens) were analysed by principal component analysis
(PCA)andcanonicaldiscriminantanalysis(CDA).Morphometricanalysesprovidedaclearseparationofthe
aphids originating from willow and those collected on grapevine. This indicates that within the Pemphigus
saliciradicisspeciestherearetwohostracesorsubspecies.
Keywords:hostrace;Vitisvinifera;morphometricvariation.
PlenaryTalk
The evolutionary ecology of symbiont-conferred resistance to

parasitoids in aphids
ChristophVORBURGER1,2
1
2
InstituteofIntegrativeBiology,ETHZurich,8092Zrich,Switzerland;(christoph.vorburger@eawag.ch)
Eawag,SwissFederalInstituteofAquaticScience&Technology,8600Dbendorf,Switzerland.
Aphidsmayharbourawidevarietyoffacultativebacterialendosymbionts.Thesesymbiontsaretransmitted
maternallywithhighfidelity,andtheyhaveroutesforhorizontaltransmissionaswell,albeitatratestoolow
to enable infectious spread. Thus, such symbionts need to provide a net fitness benefit to their hosts to
persistandspread.Somesymbiontsachievethisbyincreasingtheirhosts'resistancetoparasitoids.Drawing
on models and empirical work on the black bean aphid, Aphis fabae, its defensive symbiont Hamiltonella
defensa, and the parasitoid Lysiphlebus fabarum, I explore the evolutionary ecology of symbiontconferred
resistance to parasitoids in order to understand how symbiont frequencies are maintained at the
intermediatelevelsobservedinthefield.Ishowthatprotectionagainstparasitoidsdoesnotcomeforfree.
Hamiltonella defensa shortens the lifespan of its hosts, resulting in a significant reduction of lifetime
reproduction. Interestingly, the magnitude of this cost is determined by genotypebygenotype interactions
between host and symbiont: different aphid genotypes are unequally affected by different isolates of the
symbiont. In contrast, the benefit of harbouring H. defensa depends more on the genotypes of attacking
parasitoids. Significant interactions between H. defensa isolates and parasitoid genotypes on rates of
parasitism demonstrate a high specificity of symbiontconferred resistance, and this genetic specificity is
surprisinglyrobusttoenvironmentalperturbations.Finally,Ishowthatparasitoidsarecapableofrapidand
specific counteradaptations to overcome the resistance conferred by their hosts' symbionts. Incorporating
thesefindingsinanevolutionarymodelrevealsthatdefensivesymbiontsmayalterthereciprocalselection
between hosts and parasitoids, leading to coevolutionary dynamics that are not observed in their absence
and that may help explain the coexistence of aphids with and without protective symbionts in natural
populations.
Keywords:aphids;coevolution;costsofresistance;Hamiltonelladefensa;parasitoids;symbiosis.
The cellular immunity of the pea aphid model Acyrthosiphon pisum

and its interactions with symbionts
Antonin SCHMITZ1,2,3, Joris VILLALBA1,2,3, JeanChristophe SIMON4, JeanLuc GATTI1,2,3,

9
MarylnePOIRI1,2,3
Institut National de la Recherche Agronomique (INRA), UMR 1355 "Institut Sophia Agrobiotech" (ISA), Sophia
Antipolis,France;(marylene.poirie@sophia.inra.fr)
2
CentreNationaldelaRechercheScientifique(CNRS),UMR7254,SophiaAntipolis,France;
3
UniversitNiceSophiaAntipolis,Nice,France;
4
Institut National de la Recherche Agronomique (INRA), UMR 1349, Institut de Gntique, Environnement et
ProtectiondesPlantes,LeRheu,France.
The link between immunity and symbiosis has only recently begun to be addressed, notably in a few
invertebrate models. As aphids live in symbiosis with one obligatory and several facultative bacteria, and
different lines of identical genetic background can be produced, each one harboring a single secondary
symbiont,theyareverygoodmodelstoaddressthisquestion.Havingidentifiedandthoroughlycharacterized
the immune cells and their function in the aphid model A. pisum, we first demonstrated that primary or
secondary symbionts (SS) can be phagocytized by granulocytes and plasmatocytes, but that the observed
outcome differ between the SS. We then showed that the immune components level (hemocyte number,
phenoloxidase activity) show little variation among different A. pisum genetic backgrounds, and does not
seemtodependonwhethertheoriginoftheSSinfectionisnaturalorartificial.Strikingly,weobservedthat
the level of immune components is strongly decreased in presence of some SS, but depending on the
symbiontspecies,andevenonthestrainofthesymbiontspecies.Differenthypotheseswillbediscussedto
explainthiseffect.Altogether,ourresultsshowthatthesymbiosisofA.pisumwithsomeSSorSSstrainsmay
stronglyinfluenceitsimmunity,thuspossiblyaffectingitsimmunedefensesagainstpathogensorparasitoids.
Keywords:immunecomponents;hemocytes;phenoloxidase;secondarysymbionts;geneticbackground.
Evolutionary relationships between Pemphigini (Hemiptera: Aphididae:

Eriosomatinae) and their primary endosymbionts Buchenra aphidicola
LinLIU1,2,XingyiLI1,2,XiaoleiHUANG1,GexiaQIAO1
BeichenWestRoad,ChaoyangDistrict,Beijing100101,China;(niluil@126.com)
2
Aphids are plant sapfeeding insects that harbor primary endosymbionts called Buchnera aphidicola, in
specializedcellswithinthebodycavity.Thisancientassociationhasreceivedmuchattentionfromresearchers
interested in mechanisms involved in the symbiotic processes. The aphid tribe Pemphigini (Aphididae:
Eriosomatinae) can induce pseudogalls or galls on their primary host plants. To understand whether
relatively isolated habitats have effect on the evolutionary relationships between aphids and Buchnera, we
chose gallforming Pemphigini species on different tissues of poplar and investigated their phylogenetic
relationshipswithBuchnera.ThemolecularphylogenieswereinferredbasedonthreePemphiginigenes(COI,
COII and EF1) and two Buchnera genes (gnd, 16S rDNA), respectively, and then compared. The results
indicatedsignificantlyphylogeneticcongruenceandrevealedevidenceforparallelevolutionbetweenaphids
andBuchneraatgenericaswellasinterspecificlevels.Interestingly,theobtainedphylogeniesfrombothaphid
and Buchnera supported the sampled species into three main clades, which correspond to their feeding
locations, namely the leaf, the joint of the leaf blade and the petiole, and the branch of the host plant,
respectively.TheevolutionofgallsinPemphiginiisalsodiscussed.
Keywords:parallelevolution;molecularphylogeny;gall.
Identification of bacterial symbionts in the banana aphid (Pentalonia

nigronervosa)couplinggenomicsandproteomics
10
CarolineDECLERCK1,TsutomuTSUCHIDA3,SbastienMASSART1,PhilippeLEPOIVRE1,Frdric
FRANCIS2,HaissamJIJAKLI1
Plant Pathology Department Liege University Gembloux AgroBio Tech, Gembloux, Belgium;
(Caroline.declerck@ulg.ac.be)
2
Functional and Evolutionary Entomology Department Liege University Gembloux AgroBio Tech, Gembloux,
Belgium;
3
FrontierResearchCoreforLifeSciences,UniversityofToyama,3190Gofuku,Toyama,Toyama9308555,Japan.
TheBananaBunchyTopVirus(BBTV),transmittedbytheaphidPentalonianigronervosa,isoneofthemost
harmful viral diseases of banana and plantains. It is difficult to detect andmanage and leads to significant
productiondecline.Theexistingcontrolmethods(mainlyconsistinginthevectorchemicalcontrol)arenot
efficient enough, making this virus a serious threat for the bananaproduction worldwide. Previous studies
haveyetconsideredthepossibilityofinsectssymbiontimplicationinthetransmissionofsomeplantviruses.
But studies have been mostly related to Luteoviridae and the case of the BBTV (Nanoviridae) was never
investigated in spite of its economic importance. As a first step in the characterization of a potential
interaction between the virus and the vector, this work aimed to determine the symbiotic population of
several strains of P. nigronervosa using complementary approaches. Five aphids strains were studied: two
werecomingfromBurundi,twofromMadagascarandonefromGabon.Aphidswerecollectedinfieldsand
reared in the laboratory from a single female to obtain cloned populations. For the genomic approach,
bacterias 16S DNA regions were amplified using universal primers then sequenced. Results obtained were
confirmedusingspecificprimerstargetingtheidentifiedbacteria.Fortheproteomicapproach,2DDIGGEwas
usedinordertocheckfordifferencesinproteincontentbetweenatotalP.nigronervosaproteinextraction,a
symbiontproteinextractionfromthesameaphidandatotalproteinextractionfromanAcyrthosiphonpisum
aphidcontainingonlyB.aphidicola.Thetwoapproachesledtothesameresultsandtothedetectionoftwo
bacterial symbionts in P. nigronervosa. Phylogenetic analyses were made to replace those among other
insectsandaphidssymbionts.
Keywords:phylogeneticanalyses;phytopathology;BananaBunchyTopVirus.
Wolbachia diversity and

populations
infection
patterns
in
Chinese
aphid
ZheWANG1,XiaominSU1,2,JuanWEN1,2,LiyunJIANG1,RuiCHEN1,2,GexiaQIAO1
KeyLaboratoryofZoologicalSystematicsandEvolution,InstituteofZoology,ChineseAcademyofSciences,Beijing
100101,China;(wzhe1226@gmail.com)
2
UniversityofChineseAcademyofSciences,Beijing100049,China.
Wolbachiaisanintracellularsymbiontthatinfectsawiderangeofarthropodsandfilarialnematodes.Aphids
areengagedinhighlyinterestingandcomplexrelationshipswiththeirendosymbionts.Foursupergroups(A,B,
M,andN)ofWolbachiawerepreviously detectedinaphids,andsupergroupsMandNwereonlyfoundin
aphids. In this study, we used data from China to investigate the diversity of Wolbachia and analyze its
infection patterns in aphid species. Three supergroups (A, B, and M) were found in the examined aphid
species. Supergroup M was preponderant, whereas supergroups A and B were only detected in certain
species.SupergroupNwasnotfoundinthisstudy,andphylogeneticanalysisindicatedthatthesequencesof
supergroupNmaybelongtosupergroupB.TherewerefourinfectionpatternsofWolbachiainaphids,namely,
infectionwithsupergroupMalone,coinfectionwithsupergroupsAandM,coinfectionwithsupergroupsB
andM,andcoinfectionwithsupergroupsA,B,andM.ThepatternofinfectiononlywithsupergroupMwas
universalandwasfoundinalmostallofthesubfamiliesthatwereevaluated.Onlytwosubfamilies,Aphidinae
andLachninae,manifestedallfourinfectionpatterns.ThreepatternswereobservedinCalaphidinae(M,A&M,
B&M)andEriosomatinae(M,B&M,A&B&M).TwopatternswereobservedintheAnoeciinae(M,A&M)and
Greenideinae (M, B&M) subfamilies, and only one pattern was observed in the remaining families and/or
subfamiliesofAphidoidea.
11
Keywords:Wolbachia;aphid;diversity;infectionpattern.
Catastrophic behavior and aphid ecology: an analysis of swallowtail

model on their population dynamics
PiyaratneMKDK1,2,HuiyanZHAO1
1
StateKeyLaboratoryofCropStressBiologyinAridAreas,CollegeofPlantProtection,NorthwestA&FUniversity,
Yangling,Shaanxi,712100,China;(piyaratne.ruh@gmail.com)
2
ComputerUnit,FacultyofAgriculture,UniversityofRuhuna,Mapalana,Kamburupitiya,SriLanka.
Catastrophe movements (catastrophe phenomena) are frequent in insect ecology, especially in aphid
populations. Complexity of this phenomenon urges different modeling frameworks other than traditional
methodologies to understand the trajectories of their behavior. A few numbers of experiments have been
conductedtodevelopcatastrophemodelsinaphidecology,andmostofthemarebasedonCuspcatastrophe
theory.Populationdynamicsofaphidsoftencharacterizedthisbehaviorbuttheresearchesoncatastrophe
theory application are less documented or development has been slower. Though different literatures
suggest different reasons for this, the highlighted factors are the complexity of selecting control variables,
difficulty of understanding catastrophe regions and unclearness of explaining biological meanings. In this
paper we present a summary of existing catastrophe models in aphid ecology, examine numerous ways in
which the catastrophe can influence the efficiency of controlling aphids and present results of one of the
latestdevelopmentsofcatastropheapplicationinaphidecology.Herewefoundthatcatastrophetheorycan
be used to analyze catastrophes in aphid ecology. Some models have proved that increasing of aphid
populationisbasicallyacatastrophicbehaviorandsuddenjumpsmayoccurfromonestatetoanothereven
thoughthecontrolfactorschangesmoothly.Understandingofcatastropheregionsisalsopossiblewithfield
data,butempiricalverificationswillstillneedtobeundertakentoprovethis.Furtherwesuggestthataphid
management program developers should consider this phenomenon when they design the management
strategiesforaphidcontrolling.
Keywords:aphidcontrol;populationdynamics;catastrophetheory;cuspmodel;swallowtailmodel.
The determination of the genetic variations in Cinara cedri (Hemiptera:

Aphidoidea) populations collected on Cedrus spp. from different
locations in Turkey
HayalAKYILDIRIM1,zhanENOL2,GaziGRR2,EminDEMRTA2
1
2
ArtvinCoruhUniversity,ForestryFaculty,BotanyDepartment,Artvin/Turkey;
NigdeUniversity,ScienceandArtFaculty,DepartmentofBiology,51000,Nide.(shenol_euzhan@hotmail.com)
CinaracedriisanimportantandwidelydistributedpestspeciesfeedingonCedrusspeciesinTurkey.Tostudy
the genetic variabilities among C. cedri populations, we collected 28 populations from three different
locations (12, 4 and 12, respectively), and used random amplified polymoprhic DNA (RAPD) technique to
investigate the patterns of genetic diversity. Fifteen RAPD primers provided sufficient data. Fiftynine
polymorphic bands were identified and the overall heterozygosity was about 89.39%, which can be
consideredasimportantportionofvariability.Amongthethreepopulations,Afyonkarahisarhasthehighest
amountofpolymorphicbandwith74.24%,thishigherheterozygositymightbeexplainedwiththerelatively
diverse geography and climatic conditions. Neis genetic variation (H) for Ktahya population was 0.24, for
Uak 0.21 and Afyonkarahisar 0.27. Genetic differentiation (GST) among these populations was very low
(0.13),whilehighamountofgeneflow(Nm=3.39)wasobserved.Theamountofgeneflowamongdifferent
localitiesvaried.Forexample,Nmvaluewas7.70betweenAfyonkarahisarandKtahyapopulations,andwas
2.78betweenKtahyaandUakpopulations.Thismightbeassertedwithgeographicandclimaticvariabilities
12
in each provinces. Our findings indicate that environmental heterogeneity has nonignorable amount of
influenceongeneticvariability.
Keywords:Cinaracedri;geneticvariation;Turkey.
AuthorsthanktotheTurkishScientificCouncilforfinancialysupportingourproject(111T866).
Germline genes and germline development in the pea aphid

Acyrthosiphon pisum
Chunche CHANG1,2,3, Geeway LIN1,2, Hsiaoling LU1,2, Yimin HSIAO1,2, Chenyo CHUNG1,2,
SzuchiehWANG4,MingderLIN4
LaboratoryforGeneticsandDevelopment,DepartmentofEntomology/InstituteofBiotechnology,NationalTaiwan
University.No.27,Lane113,RooseveltRoad,Sec.4,Taipei106,Taiwan;(chunche@ntu.edu.tw)
2
ResearchCenterforDevelopmentalBiologyandRegenerativeMedicine,NationalTaiwanUniversity;
3
GenomeandSystemsBiologyDegreeProgram,NationalTaiwanUniversity;
4
DepartmentofMolecularBiologyandHumanGenetics,TzuChiUniversity,Hualien,Taiwan.
ThepeaaphidAcyrthosiphonpisum,withasequencedgenomeandaccessibletoolsforgenomicanalysis,isa
risinginsectmodelforgenomicanddevelopmentalstudies.Incontrasttotheexistinginsectmodels,thepea
aphid shows an extraordinary expansion of gene families. To date, more than 2000 gene families with
extensivegeneduplicationhavebeendiscovered,includingthegermlinetoolkitgenespiwi(Appiwi18),ago3
(Apago3a, Apago3b), vasa (Apvas14), and nanos (Apnos14). Our in situ hybridization results show that in
asexualembryosAppiwi2,Appiwi6,andApago3aarespecializedingermcellsbuttheirmostcloselyrelated
copies,Appiwi5,Appiwi3,andApago3brespectively,arenotgermlinespecific.Insexualembryos,although
expressionofAppiwi2remainsgermlinespecific,Appiwi6andApago3ahavelostthegermlineidentitythat
weobserveduringtheasexualphase.Thissuggeststhatthepeaaphidexertsdistinctrepertoiresofpiwi/ago3
expression to sustain germline development in both asexual and sexual reproduction cycles. Similarly, we
foundthatintheasexualpeaaphidexpressionsofApvas1andApnos12arerestrictedtogermcellswhilst
distributionofApvas24andApnos34mRNAsisnotgermlinespecific.Whetherthegermlinespecificidentity
ofApvas1andApnos12willbechangedinsexualembryosandhowduplicatedgermlinegenesfunctionin
asexualandsexualphasesrequiresfurtherinvestigation.
Keywords:specification;germcells;developmentalplasticity;parthenogenetic;cellfate.
Recent advances on aphid alarm pheromone biosynthetic pathway

SophieVANDERMOTEN,EricHAUBRUGE,FrdricFRANCIS
Laboratory of Functional and Evolutionary Entomology, University of Liege Gembloux AgroBio Tech, Belgium.
(Sophie.Vandermoten@ulg.ac.be)
Aphids are among the most abundant and destructive insect pests of agriculture, particularly in temperate
regions, causing direct damage to arable and horticultural crops as well as serving as vectors for many
important plant diseases. As with most other insect interactions, the signals that mediate conspecific
interactions among aphids are primarily chemical; and the most prominent and wellstudied interactions
among aphids are those mediated by alarm pheromones. The aphid alarm pheromone of which the
sesquiterpene (E)farnesene is the key (or only) component in most species plays important roles in
mediatinginteractionsamongindividualsaswellasmultitrophicinteractionsamongplants,aphids,andaphid
natural enemies. The alarm pheromones of several aphid species have been isolated and identified and,
though many important questions remain to be answered, a large body of research has addressed various
13
aspects of the biology, physiology, and ecology of aphid alarm pheromones (as recently reviewed by
Vandermoten et al. in Insect Biochemistry and Molecular Biology 42 (2012) 155163). Especially, we
contributed to recent advances in the understanding of the aphid alarm pheromone production by the
characterization of key enzymes of the biosynthetic pathway. The information gained from a deeper
understandingofthebiosynthesisandregulationofaphidalarmpheromoneaswellasthechemicalecology
ofaphidnaturalenemyinteractionwillenhanceourunderstandingofthebiologyandecologyofaphidsand
mayfacilitatethedesignofnovelcontrolstrategies.
Keywords:aphid;alarmpheromone;(E)farnesene;pheromonebiosynthesis.
Phylogeography, life cycle evolution and differentiation of sibling

species within the leaf-curling plum aphid, Brachycaudus helichrysi
(Hemiptera: Aphididae)
JosphinePIFFARETTI,FlavieVANLERBERGHEMASUTTI,EmmanuelleJOUSSELIN
INRA UMR 1062 CBGP (INRA, IRD, CIRAD, Montpellier SupAgro), Centre de Biologie pour la Gestion des
Populations, Campus International de Baillarguet CS 30 016, F34 988 MontferriersurLez, France.
(josephine.piffaretti@gmail.com)
Brachycaudus helichrysi is a worldwide polyphagous aphid pest that seriously damages its primary hosts
(Prunusspp.)andthevariouscultivatedplantsamongitssecondaryhosts(e.g.sunflower,Chrysantemum).In
a recent phylogenetic study based on worldwide sampling, we have shown that this species is actually an
amalgamation of two sibling taxa, B. helichrysi H1 and B. helichrysi H2. This study based on mitochondrial,
nuclear and Buchnera aphidicola DNA markers revealed that these two taxa display levels of genetic
divergence as great as those generally found betweensister species in the Brachycaudus genus, suggesting
that they actually correspond to two distinct species. Further investigations, based on microsatellites data
combined with ecological information suggest that these two species have two very different evolutionary
histories. B. helichrysi H1 exhibits a typical signature of a heteroecious life cycle on plum trees, and we
demonstrate the existence of distinct geographic genetic clusters within this species in the North
hemisphere.BycontrastB.helichrysiH2gathertwotypesoflineages.First,wedemonstratetheexistenceof
several persistent clonal lineages, distributed worldwide and highly polyphagous. Second, we found a
heteroeciouslineagethatusespeachtreesasprimaryhostsandhassofaronlybeenfoundinfoundinIndia.
All B. helichrysi lineages share the same secondary host range and can be found in mixed colonies on
herbaceoushostsallaroundtheworld.Wediscusshowlifecycleevolution,leadingtoreproductivebarriers,
couldexplaintheongoingspeciationintheB.helichrysispeciescomplex.
Keywords:superclones;reproductivemode;hostplantassociation;populationgenetics;speciation.
Comparative analysis of mitochondrial genomes of five aphid species

(Hemiptera: Aphididae) and phylogenetic implications
YuanWANG1,2,XiaoleiHUANG1,GexiaQIAO1
BeichenWestRoad,ChaoyangDistrict,Beijing100101,China;(wangyuan0330@163.com)
2
College of Life Sciences, University of Chinese Academy of Sciences, No. 19, Yuquan Road, Shijingshan District,
Beijing100049,China.
Insect mitochondrial genomes (mitogenomes) are of great interest in exploring molecular evolution,
phylogenetics and population genetics. Only two mitogenomes have been previously released in the insect
groupAphididae,whichconsistsofmorethan4,700knownspeciesincludingsomeagricultural,forestryand
horticultural pests. Here we report the complete 16,317 bp mitogenome of Cavariella salicicola and two
14
nearly complete mitogenomes of Aphis glycines and Pterocomma pilosum. We also present a first
comparative analysis of mitochondrial genomes of aphids. Results showed that aphid mitogenomes share
conserved genomic organization, nucleotide and amino acid composition, and codon usage features. All 37
genes usually present in animal mitogenomes were sequenced and annotated. A unique repeat region
exclusivelyinaphidmitogenomes,whichincludedvariablenumbersoftandemrepeatsinalineagespecific
manner,washighlightedforthefirsttime.Thisregionmayhaveafunctionasanotheroriginofreplication.
Phylogeneticreconstructionsbasedonproteincodinggenesandthestemloopstructuresofcontrolregions
confirmed a sister relationship between Cavariella and pterocommatines. Current evidence suggests that
pterocommatines could be formally transferred into Macrosiphini. Our research also offers methodological
instructionsforobtainingotherAphididaemitochondrialgenomes.
Keywords:aphids;Cavariellasalicicola;mitogenome;repeatregion;phylogeneticrelationship.
Phloem punctures by aphid stylets

W.FreddyTJALLINGII1,VicentaSALVADORRECATAL2
1
2
EPGsystems,Wageningen,Netherlands;(fred.tjall@epgsystems.eu)
DepartmentofMolecularPlantBiology,UniversityofLausanne,1015Lausanne,Switzerland.
Aphidstyletspenetrateplanttissuesinordertoreachaphloemsieveelements(SE)andinitiatesapfeeding.
These probing activities can be monitored by using the electrical penetration graph (EPG) technique and
therearetwoSEactivitiesreflectedasEPGwaveformsE1andE2.Fromaphidtransmissionexperimentsof
plantvirusesandaninvitrostudywithaphidsaliva(Willetal.,2007)ithasbeenderivedthatthealwaysfirst
occurringwaveformE1reflectssalivainjectionintothesieveelementtoavoidplantproteincloggingandthat
waveform E2 (following E1 or not) reflects subsequent passive sap ingestion with concurrent salivation to
keep the stylet food canal open. However, electron microscopy evidence has indicated that more SE
punctures often occur than the recorded number of E1/E2 periods (Tjallingii & Hogen Esch, 1993). This
showedthatthestyletsmaypunctureaSEwithoutanyE1/E2activities.Mostlikely,thesepunctureswere
recordedasbrief(about8s)waveformpdperiods(potentialdrop;Tjallingii,1987,Tjallingiietal.,2010)very
similar to such brief punctures in all other types of plant cells. We have not been able to discriminate
betweenbriefpuncturesintoSEsandintoothercelltypessofar.Recently,wefoundthatsomeaphidspecies
show'smallpd'waveformswithslightlydifferentfeatures.Thesesmallpdsshowalowervoltage,i.e.alower
membranepotentialofthepuncturedcellthanmostotherpds.Thesesmallpdsmayrepresentthepunctures
intosieveelementswithoutthesubsequentE1/E2phloemactivities,atleastinthreespecificaphidspecies
usedinonestudy.Inasubsequentstudyweinvestigatedwhetherotheraphidspecies,alsoshowsuchsmall
pds and what features are crucial to discriminate between small and ordinary pds. In addition to deriving
useful information about the insect activity, we recently used aphid EPGs to record electrical SE signals in
plantsrelatedtoplantwoundingbyotherinsectherbivores.MembranepotentialdepolarizationsinleafSEs
were found to depend on the magnitude of the damage and the vascular architectural relation to the
damagedleaf.Thismethodandtheresults(Salvadoretal.,PlantPhysiol;submitted)willbediscussed.
Keywords: plant penetration; phloem; sieve elements; membrane potentials; intracellular punctures; EPG
recording;electricalplantsignals.
AutoEPG analysis of the electrically monitored feeding behaviour of

aphids
TonyPRFER1,ThomasTHIEME1,W.FreddyTJALLINGII2
15
1
BTL BioTest Labor GmbH Sagerheide, RG PhytoEntomology, D18190 Gross Lsewitz, Germany;
(tt@biotestlab.de)
2
EPGSystems,NL6703CJWageningen,TheNetherlands.
AutoEPG is a new software program developed to acquire and automatically analyze the electrical
penetration graph (EPG) signals recorded when monitoring aphids probing and feeding on plants.
Characteristically EPG signals include: 1) a stylet pathway phase, waveforms A, B, C (lumped as C), which
reflectintercellularstyletpenetrationandstyletsheathsalivation(A,B,andC),andbriefcellpunctures(pd);
2)aphloemphase,waveformsE1andE2,whichreflectsalivationintophloemsieveelementsandpassive
ingestion of sieve element sap with concurrent salivation, respectively; 3) waveform F, reflecting derailed
stylet mechanics (penetration difficulties); and 4) a xylem phase, active xylem sap ingestion. Recently,
software has been developed by several researchers working in this field for distinguishing 70100 EPG
parametersfromthevisuallyanalyzedEPGsignals,whichprovidesmoredetailedinformationontheeffects
of 1) plants on plant virus transmission, 2) plant protection substances on pests and 3) the effects of the
locationofaphidresistancein(host)plants.Thesuccessoftheelectricalmonitoringtechniquearisesfrom
the fact that the EPG waveforms and the details of the patterns within these waveforms recorded for
differentaphidspeciesareverysimilar.However,thevisualanalysisofEPGwaveformsinrecordedsignalsis
verytimeconsumingandacrucialhurdletothewiderapplicationofEPG.Toovercomethiswedeveloped
AutoEPG software, which we have tested using EPG recordings of Myzus persicae, Sitobion avenae,
Nasonoviaribisnigri,andBrevicorynebrassicae.TheresultsoftheanalysisobtainedusingAutoEPGsoftware
areverycomparabletothoseobtainedusingStylet+software.AutomaticanalysesusingAutoEPGfollowed
byamanualcheckusingthefriendlycorrectionoptionresultsinaveryconsiderablesavingoftime.
Keywords:aphid;feedingbehaviour;EPG;waveforms;automaticallyanalyses.
Aphid olfaction and their potential in pest management

JingjiangZHOU
Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, Herts. AL5 2JQ,
UnitedKingdom.(jingjiang.zhou@rothamsted.ac.uk)
The sapsucking aphids are destructivepests of manyeconomically important crops throughout the world.
Like other insects, aphids have sensitive olfactory systems to detect airborne chemicals and use chemical
molecules such as speciesspecific pheromones and plant volatiles to interact with each other, host plants
andtoreacttochangesintheirenvironmentandtofindpreferredhosts,matesandovipositionsites.Mature
sexualfemalesofmanyaphidspeciesreleaseamixtureoftwoiridoidsnepetalactoneandnepetalactolwhich
act as sex pheromones to attract conspecific males. Another semiochemical which is widely used by most
aphid species is the alarm pheromone (E)farnesene which warns neighbouring aphids of attacks and
overcrowding.(E)farneseneisalsousedasaforagingcueformanyoftheaphidsnaturalenemies.Aphids
usetwoclassesofsmallsolubleproteins:odorantbindingproteins(OBPs)andchemosensoryproteins(CSPs)
tocaptureandtransportsemochemicalstoaphidolfactoryreceptors.Iwillreviewrecentresearchonaphid
semiochemicalsandolfactoryproteins,anddiscussedtheiruseinpestcontrolstrategy.
Insecticide resistance of the aphid Myzus persicae (Sulzer) in Chile
ChristianC.FIGUEROA1,2,AndreaX.SILVA1,EduardoFUENTESCONTRERAS3,LeylaCARDENAS1
InstitutodeCienciasAmbientalesyEvolutivas,UniversidadAustraldeChile,Valdivia,Chile;(alfigueroa@utalca.cl)
InstitutodeBiologaVegetalyBiotecnologa,UniversidaddeTalca,Talca,Chile;
3
FacultaddeCienciasAgrarias,UniversidaddeTalca,Talca,Chile.
Aphids have developed multiple resistance mechanisms to most insecticides. If one is able to know (1)the
2
16
genes underpinning resistance, (2) the genes being selected in resistant pest populations and, (3) the
dynamics of resistant genes over time and between crops, then better strategies to delay its spread and
prevalenceinthefieldcanbeplanned.Weinvestigatedthemolecularmechanismsofinsecticideresistanceof
M.persicae,andthedistribution,prevalenceandtemporaldynamicsofresistancegenesandaphidgenotypes
among different crops in Central Chile. First, we studied the main insecticide insensitivity mechanisms
describedtodate,verifyingthatM.persicaecarrieskdr,superkdrandMACEmutationsonpeachandpepper
cropsandtheirassociatedweeds,findinghighlevelsofresistancetoseveralinsecticidescurrentlyusedforits
control (pyrethroids and dimethyl carbamates). In addition, it was determined that carrying different
insensitivity mutations involves costs for reproduction and survival of aphids. Second, through a wide
transcriptomic survey we found a number of novel genes putatively involved in resistance, which included
genescodingforenergymetabolism,detoxification,proteintransport,extracellularpeptidasesandcuticular
proteins.Third,wefoundhighlevelsofgenotypicandgeneticdiversityinM.persicaepopulations,supporting
thepredominanceofholocycliclineagescapableofgeneratingenoughvariationtoexplaintheexpansionof
genotypes at the early season in Chile. Furthermore, we found differences in the frequency of insecticide
insensitivitymutationsaccordingtothecrop.ToexplaintheprevalenceofinsensitivitymutationsinCentral
Chile,apopulationmodelthatfavorstherapidevolutionofaphidpopulationsbypromotingthemaintenance
of insecticide resistance is proposed, which is currently being tested through a temporal study along
successiveseasons.
Keywords:aphid;insecticideresistance;populationgenetics;transcriptomics;Myzuspersicae.
FundedbyFONDECYTgrants1090378toCCF,EFCandLC.
PlenaryTalk
Bt cotton adoption promotes biocontrol services in agricultural

landscape
YanhuiLU1,KongmingWU1,NicolasDESNEUX2
1
StateKeyLaboratoryforBiologyofPlantDiseasesandInsectPests,InstituteofPlantProtection,ChineseAcademy
ofAgriculturalSciences(CAAS),China;(kmwu@ippcaas.cn)
2
FrenchNationalInstituteforAgriculturalResearch(INRA),UMR1355ISA,France.
In China, widespread adoption of Bt cotton has effectively managed cotton bollworm (CBW), Helicoverpa
armigera(Hbner)andreducedinsecticideuseonthispest.Becausebroadspectruminsecticidesnegatively
impact arthropod natural enemies, less insecticide use associated with Bt crops could have enhanced
biocontrolservicesagainstvariouspests.Ourrecentarticle(Luetal.,Nature,2012,487:362365)testedthis
hypothesis in the context of longterm landscapelevel impacts. We focused on an important pest in the
cottonagroecosystem,thecottonaphidAphisgossypii(Glover).Sincethe1980s,summeraphiddamageto
cottonincreasedinseverityandfrequencyasaresultofinsecticidespraysagainstCBW(theseinsecticides
negatively affect natural enemies). During the first years of Bt cotton adoption, we hypothesized that less
insecticideuseinBtcottonwouldfavornaturalenemieswhichsuppresstheoccurrenceofsomeinsectpests,
includingsummercottonaphidpopulations.
During19992000,fieldplotexperimentsatLangfangexperimentalstation(CAAS)indicatedthatlower
densities of predators (owing to insecticide use) allowed aphid outbreaks in insecticidetreated plots vs. in
insecticidefree plots. From 2001 to 2011, no significant difference was found for predator and aphid
abundancesbetweenBtcottonandnonBtcottonwithoutinsecticidesprays;however,predatorabundance
was significantly lower and aphid abundance was significantly higher in plots treated with insecticides for
CBWmanagementincomparisonwithinsecticidefree plots.TheseresultssuggestthatBtcottondoesnot
17
itselfaffectpredatorandaphidpopulationlevels,butBtcottonplantingefficientlypreventedcottonaphid
resurgencebydecreasinginsecticideuse.
Field surveys in 36 locations across North China during 19902010 indicated that predator population
levelsgraduallyincreasedandaphidpopulationleveldecreasedacrosstheagriculturallandscape.Regression
analysesshowedthatlessinsecticideuseagainstCBWwasadrivingfactorforenhancingpredatorpopulation
levelincotton;moreover,increasinggeneralistpredatorpopulationswerecorrelatedwithdecreasingaphid
abundance.ThesecombinedresultsindicatethatthewidespreadadoptionofBtcottonultimatelypromotes
biocontrolservicesinthecottonagroecosystembecausedecreasedinsecticideuseleadstoanincreasein
predatorpopulations.
Fieldplot trials from 2001 to 2011 indicated that there was a positive relationship between predator
abundanceincottonandsoybean,aswellasbetweencottonandpeanut.Asimilartrendwasobservedin
maizeandtheincreasedpredatorabundanceinmaizewaslinkedtoadecreaseinaphidpestabundancein
thatparticularcrop.
ThewidespreadadoptionofBtcotton,asasustainablemeasuretoreduceinsecticideuseagainstCBW,
has indirectly promoted generalist predator abundance in Bt cotton fields but also to a smaller extent in
three common adjacent crops. Our study suggests that the use of Bt crops may support conservation
biologicalcontrolatthelandscapelevel.
Keywords:aphid;generalistpredators;insecticideuse;multicropagroecosystem.
Effects of different natural enemies on population dynamics of wheat

aphids
AbidALI1,NicolasDESNEUX2,YanhuiLU1,KongmingWU1
1
ofAgriculturalSciences,Beijing100193,China;(abid_ento74@yahoo.com;kmwu@ippcaas.cn)
2
FrenchNationalInstituteforAgriculturalResearch(INRA),400RoutedesChappes,06903SophiaAntipolis,France.
The management of aphids in wheat fields relies primarily on broad spectrum chemical insecticides. These
insecticides have negative side effects on natural enemies and their extensive use often leads to the
developmentofaphidresistantpopulations.Therefore,itiscrucialtoidentifykeynaturalenemiesofaphid
pestsonwheatforoptimizingintegratedpestmanagement(IPM)packages.Afieldsurveyofthewheataphid,
Sitobion avenae and its natural enemies was conducted during the two growing seasons 20102011 and
20112012. Sitobion avenae colonized wheat in November and December but severe winter weather
preventeddamagetothecrop.Duringthespring,aphidpopulationsincreasedslowlyandpeakedat~2300
(May 23rd 2011) and ~5000 aphids (May 17th 2012) per 100 wheat tillers. The aphid population declined
during2012whilein2011itreachedasecondminorpeakinthefirstweekofJune.Themainnaturalenemies
recordedwereaphidparasitoidswithAphidiusgifuensisandScelionidaespp.dominantin2011,whilein2012
Diaeretiella rapae and A. gifuensis were dominant. By contrast, we observed few predatory coccinellids.
Sitobion avenae populations were two to three times higher in smallmesh cages (exclusion of all natural
enemies) than populations in largemesh cages (exclusion of large predators e.g. coccinellids) and seven to
tentimeshigherthanonuncagedplants.Theseresultsconfirmedthataphidparasitoids,mainlyA.gifuensis,
arethekeynaturalenemiesofS.avenaeonwheatinNorthernChina.Furtherstudieswillaimatassessing
side effects of pesticides on the key aphid parasitoid A. gifuensis to enable integrating biocontrol services
providedbythisparasitoidinIPMstrategiesinwheat.
Keywords:parasitoid;integratedpestmanagement;naturalenemyexclosureexperiment;Sitobionavenae.
Nonlinearities lead to qualitative differences in population dynamics of

predator-aphid systems
OlgaM.C.C.AMEIXA1,GerbenJ.MESSELINK2,PavelKINDLMANN1,3
18
1
Department of Biodiversity Research, Global Change Research Centre, esk Budjovice, Czech Republic;
(pavel.kindlmann@centrum.cz)
2
WageningenURGreenhouseHorticulture,Bleiswijk,TheNetherlands;
3
InstituteforEnvironmentalStudies,CharlesUniversity,Prague,CzechRepublic.
Sincetypicallytherearemanypredatorsfeedingonmostherbivoresinnaturalcommunities,understanding
multiplepredatoreffectsiscriticalforbothcommunityandappliedecology.Experimentsofmultiplepredator
effectsonpreypopulationsareextremelydemanding,asthenumberoftreatmentsandtheamountoflabour
associatedwiththeseexperimentsincreasesexponentiallywiththenumberofspeciesinquestion.Therefore,
researchers tend to vary only presence/absence of the species and use only one (supposedly realistic)
combination of their numbers in experiments. However, nonlinearities in density dependence, functional
responses,interactionsbetweennaturalenemiesetc.aretypicalforsuchsystems,andnonlinearmodelsof
populationdynamicsgenerallypredictqualitativelydifferentresults,ifinitialabsolutedensitiesofthespecies
studied differ, even if their relative densities are maintained. Therefore, testing combinations of natural
enemieswithoutvaryingtheirdensitiesmaynotbesufficient.Herewetestthispredictionexperimentally.We
show that the population dynamics of a system consisting of 2 natural enemies (aphid predator Adalia
bipunctata(L.),andaphidparasitoid,AphidiuscolemaniViereck)andtheirsharedprey(peachaphid,Myzus
persicaeSulzer)arestronglyaffectedbytheabsoluteinitialdensitiesofthespeciesinquestion.Eveniftheir
relativedensitiesarekeptconstant,thenaturalenemyspeciesorcombinationthereofthatmosteffectively
suppresses the prey may depend on the absolute initial densities used in the experiment. Future empirical
studiesofmultiplepredatoronepreyinteractionsshouldthereforeuseatwodimensionalarrayofinitial
densitiesofthestudiedspecies.Varyingonlycombinationsofnaturalenemieswithoutvaryingtheirdensities
isnotsufficientandcanleadtomisleadingresults.
The interspecific competition of Bemisia tabaci B biotype and Myzus

persicae mediated by cabbage
ChengQU,AihongLIU,MingXUE
College of Plant Protection, Shandong Agricultural University, Taian 271018, China. (abcdef98765432@sina.com;
xueming@sdau.edu.cn)
ThewhiteflyBemisiatabaciBbiotypeisaworldwideinvasivepest.Fieldstudiesfoundthatcabbageplants
preinfested by whiteflies had fewer Myzus persicae than those without whiteflies. Pot experiment and
clipcage methods were used to determine the interaction between whiteflies and aphids on cabbage.
PresenceofwhiteflieshadasignificantinhibitoryeffectonpopulationofM.persicae.Thepopulationnumber
ofM.persicaeinthesecondgenerationdecreasedby74.65%andsurvivalrateofM.persicaedecreasedby
19.48% on the second day; Leaf position had a significant effect: the inhibitory effects on systemic leaves
were significant, whilst inhibition on the interior leaves and local damaged leaves were not. Cabbage
preinfested by M. persicae had no adverse effects on the survival rate, development and fecundity of
whiteflies.ThecabbagedefenseresponseinducedbyB.tabaciwasspecifictoM.persicae,whichplayedan
importantroleininterspecificcompetitionbetweenB.tabaciandM.persicae.
Keywords:aphid;development;interspecificcompetition;inducibledefense;whitefly.
ThisworkwasfinanciallysupportedbythenaturesciencefoundationofChina(30971906).
Defensive behavior of aphids against parasitoids exhibiting host

fidelity
ClaudioC.RAMREZ,SebastinORTZ,BlasLAVANDERO
LaboratoriodeInteraccionesInsectoPlanta,InstitutodeBiologaVegetalyBiotecnologa,UniversidaddeTalca,2
19
Norte685,Talca,Chile.(clramrez@utalca.cl)
Thedisplayofdefensivebehaviorsbyaphidsdependsondifferentinternalandexternalfactors.Amongthe
externalfactors,theeffectofhostfidelityoftheattackingparasitoidsisnotwellunderstood.Inthisstudy,the
parasitoid Aphidius ervi was reared for many generations on three different aphid species (Acyrthosiphon
pisum, Sitobion avenae and Rhopalosiphum padi) and the parasitization behaviors of parasitoids and the
defensivebehaviorsofeachaphidspeciesconfrontingnatal(rearedonthesameaphidspecies)andnonnatal
(different to the reared aphid species) parasitoids were recorded. Whilst in all the species the parasitoids
performed more efficient attacks on natal aphids than nonnatal ones, the aphid defensive response in
generalwasnotrelatedtotheparasitoidnatalhost.However,foronecloneofthepeaaphid(A.pisumalfalfa
clone)therewasahigher frequencyof walkingawayandkickingwhenconfrontedwithanA.pisumalfalfa
clone originatedparasitoid. Therefore, although host fidelity of parasitoids confers greater efficiency when
handlingandforagingontheirnatalaphid,withtheexceptionoftheabovementionedA.pisumclone,thisis
notassociatedwithagreaterdefensiveresponseofaphidsagainsttheparasitoidsattack.
Keywords:aphid;parasitoid;defensivebehavior;hostfidelity;parasitization;evolution.
Polyphagism of Stomaphis wojciechowskii Depa (Aphididae,

Lachninae) ecological importance and significance for species
recognition
ukaszDEPA,EwaMRZ
UniversityofSilesia,Bankowa9,40007Katowice,Poland.(lukasz.depa@us.edu.pl)
Species of the genus Stomaphis, remarkable for their large body size and extremely long rostrum, are
regardedasmonophagous,feedingontrunksofvariousspeciesoftrees,alwaysattendedbyants.Stomaphis
wojciechowskiiisarecentlydescribedspecies,whichhasnotpreviouslybeendistinguishedfromS.quercus.
However,detailedstudyofitsecology,includinganalysiswithmolecularmarkersCOIandCOII,revealedits
distinctionfromthelatter.Thespeciesdiffermorphologicallyandalsowithrespecttotheirmutualisticant
species,whichfurtherinfluencestheirecology.Stomaphisquercus,livingwithLasiusfuliginosus,isasurface
feederonQuercusspp.andBetulaspp.,whileS.wojciechowskiiisassociatedwithL.brunneus,whichlives
mainlyunderthebarkoftrees,areasonwhyS.wojciechowskiiremainedunrecordedforsolong.Extensive
fieldresearchrevealeditspresencenotonlyinmanyterritoriesofCentralEurope,butalsoonmanydifferent
host plants, such as Q. robur, Q. petrea, Q. cerris, Alnus glutinosa, Juglans regia and Tilia cordata, always
deep under the bark in chambers of L. brunneus. The host plant genera, except for Juglans comprise the
dominantspeciesofdeciduousforestsofEurope.Itisconcludedthatthepolyphagismofthisspeciesisan
adaptation to the habitat of deciduous forests and mutualistic relationship with L. brunneus. The issue of
Stomaphisspeciesrecognitiononbasisoftheirrelationshipwithhostplantspeciesisalsodiscussed.
Keywords:habitattype;hostspecificity;antmutualism.
Life table evaluation of survival and reproduction of the aphid,

Sitobion avenae (Hemiptera: Aphididae), exposed to cadmium
Huanhuan GAO, Huiyan ZHAO, Chao DU, Mingming DENG, Erxia DU, Zuqing HU, Xiangshun
HU
Institute of Plant Protection, College of Plant Protection, Northwest Agriculture and Forest University, State Key
LaboratoryofCropStressBiologyinAridAreas,Yangling,Shaanxi712100,China.(gaohuanhuan368@126.com)
Theeffectsofcadmiumexposure(Cd)onthedevelopment,fecundity,andreproductionofthegrainaphid,
20
Sitobionavenae(Fabricius)wereestimatedbyconstructingalifetable.TheconcentrationsofCdinthesoil
were:0,10,20,40,80,and160mg/kg.CadmiuminwheatincreasedwiththeincreaseofCdconcentrations
insoil.ThelatterpartofthereproductionperiodwassignificantlyaffectedbyCd,accordingtothecurveof
thetotalsurvivalrate(lx).Thenetreproductiverate(R0),innatecapacityofincrease(r),andfiniterateof
increase()ofS.avenaealldecreasedunderthestressofCd,andwerelowestataCdconcentrationof20
mg/kg. Cd also negatively affected fecundity and mx (the number of offspring produced by an individual
female).At20mg/kg,thedeclineofthemwasmostobvious.Inconclusion,survivalandreproductionofS.
avenaewereinhibitedunderthetreatmentoftheheavymetalCd.SitobionavenaewasmoresensitivetoCd
at concentration of 20 mg/kg compared to the other concentrations. This concentration can be used to
examinethemechanismsbehindpopulationgeneticsandbiologicalmutationofS.avenaewhenexposedto
heavymetal.
Keywords:heavymetal;wheat;fecundity.
Myzus ascalonicus in the sub-Antarctic: a story of a biological

invasion
MauriceHULL
InstituteofGenetics,EnvironmentandPlantProtection(IGEPP),NationalInstituteofAgronomicResearch(INRA),
BP35327,35653LeRheu,France.(Maurice.hulle@rennes.inra.fr)
The subAntarctic islands form a loose ring within the latitudinal belt 4554S. They are among the most
isolated islands from continental landmasses and contain a limited number of habitats. There are very few
native species of flora and invertebrates, with a relatively high number of decomposers and very few
herbivoresorpredators.Exoticplantsandinvertebrateshavebeenintroduced(especiallysincemanhasset
up permanent bases), drastically modifying ecosystem function. 18 aphid species have been recorded on
these islands(from 0 to 9per island, depending on mean annual temperature).With the exception of two
endemic Pentamyzus species on the Falklands, all other species are cosmopolitan and have been recently
introduced.Thesespeciesarepolyphagousandreproduceasexually.Sixaphidspecieshavebeenintroduced
onKerguelenIslands.Threeofthemarenaturalizedandone,Myzusascalonicus,isinvasive.Thisspecieshas
beenrecordedinalmostalllittoralhabitatsonKerguelenon26differenthostplantsbelongingto14families
andincludingsixnativeplants.Measuresofperformance(multiplicationrate,developmentaltime,survival,
etc.) are higher on native plants than on exotic plants. This result is discussed in light of the possibility of
ineffective or absent defense mechanisms in native plants which have evolved without sapfeeding insects.
Under current temperature conditions (mean annual temperature is 4.8 C), M. ascalonicus colonies are
mostly found at short distance from the seashore within the first 150 m above sea level. With increasing
temperature, the species could expand its range and therefore take advantage of global warming. The
downside of this invasive success is that M. ascalonicus may not be healthy: 90% of alates have defective
wingssuggestingdevelopmentalproblems.Withonlyonecloneandwithoutanygeneticinflow,thequestion
ofitsadaptabilitytolocalconditionsisasked.
Keywords:aphid;biologicalinvasion;plantinsectinteractions;KerguelenIslands.
Metacommunity ecology of specialized tansy aphids
MohsenMEHRPARVAR,WolfgangW.WEISSER
TerrestrialEcology,DepartmentofEcologyandEcosystemManagement,TechnischeUniversittMnchen,D85354
Freising,Germany.(mohsen.mehrparvar@tum.de;mehrparvar@aphidology.com)
21
Ametacommunityisdefinedasasetoflocalcommunitiesthatarelinkedbydispersalofmultiple,potentially
interacting, species i.e. a multispecies metapopulation. Dispersal, colonization and extinction are the core
processes that shape and structure metacommunities. In a set of experiments, we used three specialized
tansy aphid species, Macrosiphoniella tanacetaria, Metopeurum fuscoviride and Uroleucon tanaceti, to
investigate the effects of biological interactions and environmental factors on the metacommunity core
processes. Seasonal timing, mother morph, crowding and predators were found to have effects on the
production of winged dispersal morphs of M. tanacetaria; but for the anttended M. fuscoviride seasonal
timing and not crowding or predator presence affected wing production. We found that the presence of
mutualisticantsgreatlyalteredcompetitivehierarchiesamongtheaphidspeciesandtheeffectofpredators
onthepersistencetimeofaphidcolonies.Thethreetansyaphidspeciescolonizedparticularplantsmostlyto
avoid futurecompetitiveinteractions.Selectionofplantsbywingedaphidswasinfluencedbythepresence
andpreviousoccupationofotheraphidsonthehostplantsothatthisbehaviourmayleadtodifferentcolony
compositions in the field. Tansyaphid metacommunity dynamics can be described mostly by the
speciessortingperspectiveinwhichdifferentaphidspeciessortintodifferenthabitatsthatcreateseparate
spatialnichesthroughhabitatheterogeneity.
Keywords:aphid;colonization;dispersal;extinction;metapopulation.
Plum (Prunus spp.) aphid guild structure in the Eastern Baltic region
of Europe
RimantasRAKAUSKAS,AudriusZAREMBA
Department of Zoology, Vilnius University, M. K. iurlionio 21/27, LT03101, Vilnius, Lithuania.
(rimantas.rakauskas@gf.vu.lt)
Eight species of the aphid family Aphididae feed on plums (Prunus spp.) in the Eastern Baltic region:
Brachycaudus divaricatae, B. cardui, B. helichrysi, Hyalopterus pruni, Phorodon humuli, Rhopalosiphum
nymphaeae,R.padiandAphisspiraecola.Theaphidguildstructureofcherryplum(P.cerasifera)isdifferent
from that of domestic plum (P. domestica). The principle aphid species inhabiting cherry plum was B.
divaricatae(81%ofallinfestedplants,93%ofheavilyinfestedplants),whilstthatondomesticplumwasH.
pruni(72%ofallinfestedplants,96%ofheavilyinfestedplants).Brachycaudusdivaricataewasmonoecious
on cherry plum. Hyalopterus pruni was capable of feeding on both species of plum. Seven species were
capable of coexisting with the dominant species B. divaricatae on cherry plum, whilst the aphid guild of
domesticplumconsistedoffourspeciesonly(H.pruni,B.cardui,B.helichrysiandP.humuli).Coexistenceof
aphidspeciesonplumsintheEasternBalticregionispossiblycontrolledbyresourcepartitioning(different
aphid species prefer specific positions on the host plant) and different life cycle strategies (heteroecious
speciesB.cardui,B.helichrysi,P.humuli,R.nymphaeaeandR.padileavefortheirsummerhostsearlyinthe
season).Despitetheavailabilityofthesummerhost(commonreedPhragmitesaustralis)H.pruniwasableto
thriveonplumsthroughouttheseasonthusshowingfacultativeheteroecy. Hyalopteruspruniwastheonly
species capable of forming mixed colonies with B. divaricatae, although in most cases the former species
prefersplumfoliage,whilstthelatterprefersyoungshootsandrootsuckers.
Keywords:aphid;hostassociation;guildstructure;resourcepartitioning;specialization.
Correlations among growth and reproduction parameters for three

cereal aphids on ten wheat lines
XiangshunHU,XiaofengLIU,ZuqingHU,HuiyanZHAO,TongxianLIU
KeyLaboratoryofCropPestManagementontheNorthwestLoessPlateauofMinistryofAgriculture,andStateKey
Laboratory for Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100, China.
(huxiangshun@163.com)
22
Selected paired correlations between five growth parameters: i) Development Time (DT), ii) Weight Gain
(WG),iii)Fecundity(F),iv)IntrinsicRateofIncrease(rm)andv)MeanRelativeGrowthRate(MRGR)ofthree
cerealaphidspecies:Sitobionavenae,RhopalosiphumpadiandSchizaphisgraminumontenwheatlineswere
evaluated.ThecorrelationsintheresponsesofS.avenaeweresignificantbetweenbothMRGR,WGandboth
F and rm, and not significant between DT and other three parameters across the ten wheat lines; and
significantlybetweenrmandbothMRGRorWGwithinalltenwheatlines.Thecorrelationsintheresponses
ofR.padiweresignificantbetweenDTandbothrm,F,WGandFacrossthetenwheatlines,andbetweenrm
and both MRGR, DT within all ten lines. The correlations in the responses of S. graminum were significant
betweenalmostalltheparametersacross/withintenwheatlines.Theseresultssuggestthattheresponsesof
S.graminumtoresistanceofwheatlinesareuniversal,butthatdifferentmechanismsarebasedonoperating
accordingtothewheatlineonwhichS.avenaeandR.padiareplaced.S.avenaeandR.padihaveahigher
degree of phenotypic plasticity and can adapt to different wheat lines through the tradeoff between life
historycharacters.
Keywords:hostplantresistance;developmenttime;weightgain;fecundity;intrinsicrateofincrease.
Pea aphid promotes amino acid metabolism both in Medicago

truncatula and bacteriocytes to favor aphid population growth under
elevated CO2
YuchengSUN1,HuijuanGUO1,2,FengGE1
State Key Laboratory of Integrated Management of Pest and Rodents, Institute of Zoology, Chinese Academy of
Sciences,Beijing100101,China;(sunyc@ioz.ac.cn)
2
GraduateSchool,ChineseAcademyofSciences,Beijing100039,China.
RisingatmosphericCO2levelscandilutethenitrogen(N)resourceinplanttissue,whichisdisadvantageousto
many herbivorous insects. Aphids appear to be an exception that warrants further study. The effects of
elevatedCO2(750ppmvs.390ppm)wereevaluatedonNassimilationandtransaminationbytwoMedicago
truncatula genotypes, a Nfixingdeficient mutant (dnf1) and its wildtype control (Jemalong), with and
without pea aphid (Acyrthosiphon pisum) infestation. Elevated CO2 increased population abundance and
feedingefficiencyofaphidsfedonJemalongbutreducedthoseondnf1.Withoutaphidinfestation,elevated
CO2 increased photosynthetic rate, chlorophyll content, nodule number, biomass and pod number for
Jemalong, but only increased pod number and chlorophyll content for dnf1. Furthermore, aphid infested
Jemalong plants had enhanced activities of N assimilationrelated enzymes (GS, GOGAT) and
transaminationrelatedenzymes(GOT,GPT),whichpresumablyincreasedaminoacidconcentrationinleaves
and phloem sap under elevated CO2. In contrast, aphid infested dnf1 plants had decreased activities of N
assimilationrelated enzymes and transminationrelated enzymes and amino acid concentrations under
elevated CO2. Furthermore, elevated CO2 upregulated expression of genes relevant to amino acid
metabolisminbacteriocytesofaphidsassociatedwithJemalong,butdownregulatedthoseassociatedwith
dnf1. Our results suggest that pea aphids are able to manipulate their interactions with their hosts and
promote amino acid metabolism in both the host plant and in its bacteriocytes to favor the population
growthoftheaphidunderelevatedCO2.
Keywords:Acyrthosiphonpisum;bacteriocyte;biologicalnitrogenfixation;elevatedCO2.
Immunolocalization of odorant-binding proteins on

chemosensilla of the peach aphid Myzus persicae (Sulzer)
antennal
LipingBAN1,YinpengSUN2,ShanganZHANG3
23
1
College of Animal Science & Technology, China Agricultural University, Beijing 100193, China;
(lipingban@cau.edu.cn)
2
CollegeofAgricultureandBiotechnology,ChinaAgriculturalUniversity,Beijing100193,China;
3
InstituteofZoology,ChineseAcademyofSciences,Beijing100101,China.
The antennal chemosensilla of Myzus persicae were mapped using scanning and transmission electron
microscopy. Primary rhinaria were found on the 5th and 6th segments, and two types of trichoid sensilla
were present all through the length of the antenna. Secondary rhinaria were present on the 3rd antennal
segment of alate aphids. The ultrastructure of placoid sensilla revealed the presence of 24 groups of
neuronsandmultipleporesonthesurface.Coeloconicsensillahouse2dendritesextendingtothepeg,but
donotpresentanyporeatthetip.TrichoidsensillaoftypeIareinnervatedbysingleneurons,endingina
tubular body beneath the hair; those of type II are innervated by two to four neurons, some reaching the
distalendofthehair.Immunocytochemicallocalizationofodorantbindingproteins(OBPs)wasperformed
onultrathinsectionsofantennalchemosensilla.TheantiserumagainstOBP7intensivelylabeledallplacoid
sensillafrombothprimaryandsecondaryrhinaria,withgoldgranulesconcentratedinthelymphsurrounding
thedendrite.OBP6andOBP3couldalsobedetectedinplacoidsensilla,butlessstronglythanOBP7.Barely
significantreactionornoreactionwasobservedwithantibodiesagainstOBP8.
Keywords:chemosensilla;Myzuspersicae;odorantbindingprotein;ultrastructure;immunocytochemistry.
The effects of enhanced Ultraviolet-B radiation on the biology of green

and brown morphs of Sitobion avenae (Hemiptera: Aphididae)
ZuqingHU1,HuiyanZHAO1,ThomasTHIEME2
StateKeyLaboratoryofCropStressBiologyinAridAreas,NorthwestA&FUniversity,Yangling,712100,Shaanxi,
China;(zhaohy@nwsuaf.edu.cn)
2
BTLBioTestLaborGmbHSagerheide,Birkenallee19,18184sagerheide,Germany.
TheeffectsofenhancedUltravioletB(UVB)radiationonthebiologyofgreenandbrownmorphsofSitobion
avenae(Fabricius)weretestedunderlaboratoryconditions.ThetwoS.avenaemorphsweredirectlyexposed
todifferentdoses[0,216,432,and864kJ/m2]ofartificialUVB.UnderlowdosesofUVB(216kJ/m2),the
nymphaldevelopmentperiodwassignificantlyshorter,whilemeanrelativegrowth,totalfecundity,andgross
reproductive rate were significantly higher for both aphid morphs compared with those under control
treatments (0 kJ/m2). Under high doses of UVB (432 kJ/m2 and/or 864 kJ/m2), the nymphal development
periodwassignificantlylonger,whilethereproductiveperiod,postreproductiveperiod,differenceinweight,
meanrelativegrowth,andlifetableparametersweresignificantlyreducedforbothaphidmorphs.Moreover,
the nymphal development period was significantly shorter and the differences in weight, mean relative
growth,totalfecundity,andlifetableparametersweresignificantlyhigherforthebrownmorphunderhigh
doses of UVB compared to the green morph. The results show that enhanced UVB negatively affects the
performance of the green and brown S. avenae morphs. The brown morph exhibited stronger adaptability
thanthegreenmorphathighdosesofUVB.
Keywords:Sitobionavenae;artificialUVBradiation;colormorph;development;reproduction.
The effects of increased UV-B radiation on survival, development, and

gene expression in Sitobion avenae
JieYANG,LiZHANG,HuanhuanGAO,DongZHOU,HuiyanZHAO
StateKeyLaboratoryofCropStressBiologyforAridAreas,CollegeofPlantProtection,NorthwestA&FUniversity,
Yangling,Shaanxi712100,China.(casc007@163.com)
24
Enhanced level of UVB radiation due to anthropogenic activities is deteriorating the environmental
conditionsnecessaryforgrowthanddevelopmentofplantsandherbivores.ToexaminetheeffectsofUVB
radiationonthesurvivalanddevelopmentofgrainaphid,Sitobionavenae(Fabricius),animportantpestof
cereals, three UVB intensities including elevated (0.75W/m2), normal environmental (0.20W/m2) and UVB
filtered were used. The results indicated that the effects of three UVB intensities on fecundity, net
reproductiverate,intrinsicrateofincreaseandfiniterateofincreasewerenotsignificantlydifferent.However,
when exposed to normal ambient UVB dose, mean generation time significantly shortened, and nymph
survival rate was higher than other UVB doses; also development of the second and fourth instar nymphs
wassignificantlyshorterthanthatexposedtoelevatedradiatedcohorts.Togainmoreinsightintomolecular
mechanisms underpinning the UVB radiation response, we performed qPCR reactions on four UV stress
associated genes, Mre11, Chitinase3, Hsp70b2 and Hsc70b. Expression of Mre11 was strongly induced by
UVBradiation,andtheexpressionlevelsofChitinase3andHsp70b2wereUVBdosedependent.However,
theexpressionofHsc70bwasnotaffectedbyUVBintensities.Inconclusion,highintensityUVBaffectsthe
development and gene expression of the grain aphid. However, aphid growth was promoted under low
intensityUVB.
Keywords:ultraviolet;heatshockproteins;MRNcomplex;heatshockcognateprotein;lifetable.
PlenaryTalk
Resistance to aphids in a model plant, Medicago Truncatula

Karam B. SINGH1,2, Lars G. KAMPHUIS1,2, Katherine ZULAK1, Sumin GUO1, Judith
LICHTENSVEIG1,OwainEDWARDS3,JonathanANDERSON1,LinglingGAO1
1
CSIROPlantIndustry,Floreat,WesternAustralia,Australia;(karam.singh@csiro.au)
UniversityofWesternAustralia,Crawley,Australia;
3
CSIROEcosystemScience,Floreat,WesternAustralia,Australia.
We are interested in two plant defence to aphids and related phloemfeeding insects, which cause severe
plant damage, through feeding activities and as vectors of plant viruses. A focus has been on resistance to
bluegreen aphid (BGA) where we have fine mapped a dominant gene conditioning resistance called AKR
(Acyrthosiphon kondoi resistance). Targeted approaches to identify signaling pathways and genomic
approachesinvolvingtranscriptomicsandmetabolomicsarehelpingunderstandhowthisgeneconfersaphid
resistance, through the identification of signaling pathways, transcription factors and key metabolites
important for AKR mediated resistance. Progress is also being made in identifying other aphid resistance
genesinM.truncatulaincludingsingledominantresistantgenesagainstspottedalfalfaaphid(SAA)andpea
aphid(PA).WehavealsocharacterisedmoderateformsofresistancetoBGA,PA,SAAandcowpeaaphidthat
aregeneticallymorecomplexandmediatedthroughmultipleQTLs.
2
Generation of a novel type of transgenic wheat emitting aphid alarm

pheromone for aphid control
Xiudao YU1, Jia FAN2, Min ZHANG1, Hui WANG1, Yi HE1, Genping WANG1, Youzhi MA1, Julian
CHEN2,YongjunZHANG2,LanqinXIA1
1
Institute of Crop Science/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese
Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing 100081, China;
(xialanqin@caas.cn)
25
2
InstituteofCropProtections,ChineseAcademyofAgriculturalSciences(CAAS),Beijing100193,China.
Aphidsaremajoragriculturalpestswhichcausesignificantyieldlossesofwheateachyear.Asthemainand
generallyonlycomponentoftheaphidalarmpheromones,(E)farnesene(EF)isinvolvedinthechemical
communication between aphids and particularly in the avoidance of their enemies. In this study, an EF
synthasegenefromAsianpeppermintwereisolatedanddesignatedasMaFS1.Functionalstudyofthisgene
intobaccoindicatedthattransgenictobaccoplantsoverexpressingthisgenecouldemitEFandcausethe
repellencebehaviorofaphid(Myzuspersicae).TransgenicwheatexpressingMaFS1underthecontrolofa
greentissue specific rice Rubisco small subunit promoter (rbcS) was generated through
Agrobacteriummediatedtransformation.ResultsofPCRandSouthernblotanalysesindicatedthatMaFS1
was stably integrated into the genome of the transgenic wheat and could be inherited in the following
generations.QRTPCRanalysisdemonstratedthattheexpressionofMaFS1waslimitedtothegreentissues
of transgenic plants, with less or even no detectable expression in immature and mature seeds.
Overexpression of MaFS1 in wheat plants resulted in the emission of EF ranging from 2.09 to 4.65
ng/day/gfreshtissues,thusconferredrepellenceofSitobionavenaeinthetransgenicwheatlinescompared
with the nontransgenic control. These results suggested that EF synthase manipulation was a valuable
alternativestrategyforminimizingaphidinfestationinwheatplantsinanenvironmentallybenignway.
Keywords:wheat;aphid;EFsynthasegene;RbcSpromoter;alarmpheromone.
Effects of genetic resistance combinations in melon on density and

genetic diversity of Aphis gossypii populations in fields
SophieTHOMAS1,FlavieVANLERBERGHEMASUTTI1,NathalieBOISSOT2
1
INRA, UMR1062 CBGP, Campus international de Baillarguet, CS 30016, F34988 MontferriersurLez, France;
(sophie.thomas@supagro.inra.fr)
2
InstitutNationaldelaRechercheAgronomique(INRA),UR1052,UnitdeGntiqueetdAmliorationdesFruitset
Lgumes,DomaineStMauriceAlledeschnes,CS60094,F84143Montfavetcedex,France.
Since the deployment of genetic resistant cultivars, several cases of breakdown have occurred rising the
question of durability. Quantitative resistances are considered broadspectrum and more durable than
qualitativeresistancesbutthishasbeenpoorlytested.Westudiedselectionpressuresexertedbyqualitative
andquantitativeplantresistancesinfieldsintheaimtounderstandhowlocaladaptationofpestpopulations
couldoccurinagrosystemsandtodevelopstrategiesforthemanagementofresistances.Fieldtrialswereset
up in three areas with different melon resistance combinations in a homogeneous genetic background,
associatingamajorgene(theVatgene)andquantitativetraitloci(QTLs)knowntoaffectplantacceptancein
twoclonesofAphisgossypii.EffectsoftheVatgeneandtheQTLsonthedensityandthegeneticdiversityofA.
gossypii field populations were analyzed. Among the 138 multilocus genotypes (MLGs) identified in 2772
aphidscollected,only11developedcolonies.TheVatgenereduceddensityofaphidpopulationsonmelon
cropsinallareasandresultedinchangesoftheclonaldiversityandthegeneticstructureofaphidpopulations;
theclonalrichnesswasreducedto6MLGsincoloniescollectedonVatplants.Conversely,theQTLsdidnot
affect aphid density and slightly affected genetic diversity. Along with selection, competition and local
extinctionprobablyconditionemergenceofcertainMLGsonVatplants.Becauseoftheirnarrowspectrum,
the QTLs tested did not reduce the risk of emergence of MLGs adapted to the Vat gene when these MLGs
wereassociatedtotheVatgene.
Keywords:melonaphid;populationgenetics;QTL;resistancedurability;Vatgene.
The studies on the crop resistance to aphid

Huiyan ZHAO1, Thomas THIEME2, Udo HEIMBACH3, Zuqing HU1, Xiangshun HU1, Kun LUO1,
ChunpingWANG1
26
1
StateKeyLaboratoryofCropStressBiologyinAridAreas,CollegeofPlantProtection,NorthwestA&FUniversity,
Yangling,Shaanxi,712100,China;(Zhaohy@nwsuaf.edu.cn)
2
BTLBioTestLaborGmbHSagerheide,D18184Sagerheide,Germany;
3
JuliusKhnInstitu(JKI),D38104Braunschweig,Germany.
Toscreenresistancevarietiesofcropsandinsurecropyieldandquality,byusingmethodssuchasMRGR,Life
Table,RTPCR,thewheatgermplasmswasscreened;screeningresistancegenesinnearisogenicpool,EPGto
analysisphysicalresistanceloci.Theresultsshowedthatthe2340wheatgermplasmmaterialswerescreened.
23resistancevarietieswerefound,wherein,resistanceonaphidofXZseriesvarietiesandanti30,anti35is
significant. WDV stimulate Sitobion avenae feeding and BYDV inhibitory effects. The 4 genes of resistance
aphidinwheat7DLchromosome,theirgeneticdistanceare6.3cM,15.1cM,19.1cMand34.1cM.Resistant
geneislocatedonchromosome7DLinAnti35,namedSa1.Thedifferentialexpressionsofwheatresistance
geneticonS.avenaeweredividedintofivemodels.EPGresultsshowedthatresistancesitesonS.aveanaein
Ww2730 locate in the epidermis, Xiaoyan22 to Rhopalosiphum padi resistance loci locate in cell wall. Our
studyindicatesthattheresistancevarietiesandresistantlocationsandmechanismsvary.S.avenaehasbetter
adaptabilityonwheatinfectedbyWDVinsteadofBYDV.
Keywords:wheat;aphids;resistance;MRGR;EPG;genelocation.
Resistance of the bioenergy crop miscanthus towards the corn leaf

aphid, Rhopalosiphum maidis (Fitch) (Hemiptera: Aphididae)
ArnaudAMELINE1,SophiePOINTEAU1,EurielJAGUENET1,CarolineRAMBAUD2,AudeCOUTY1
1
FRE 3498 EDYSAN (Ecologie et Dynamique des Systmes Anthropiss), Laboratoire de BioEcologie des Insectes
PhytophagesetEntomophages,UniversitdePicardieJulesVerne,33rueStLeu,F80039AmiensCedex,France;
(arnaud.ameline@upicardie.fr)
2
UMRINRA1281,Stressabiotiquesetdiffrenciationdesvgtauxcultivs,UniversitLilleNorddeFrance,Lille1,
BtimentSN2,F59650VilleneuvedAscqCedex,France.
TheperennialC4grassesofthegenusMiscanthusoriginatefromEasternAsiaandcompriseaboutadozen
grassspeciesamongwhichMiscanthussinensis,M.sacchariflorusandtheirtriploidsterilehybridMiscanthus
xgiganteushaveattractedmuchattentionaspotentialbiomasscropsduringthe1990s.Theintroductionof
suchanexoticbiofuelcropmayhaveimportantconsequencesontheequilibriumoflocalagrosystems.One
nonnegligibleriskisthatbioenergycropsmayactasreservoirsofseriouspestsanddiseasesforothercrops.
Thus, plant resistance to insect pests is one important component of integrated control. As colonies of the
aphid Rhopalosiphum maidis have been observed on miscanthus in the USA, it is essential to study its
colonizationprocessonthisbioenergyculturewhichhasalsobeenrecentlygrowninPicardie(France).This
study has investigated the type of Miscanthus resistance (antibiosis or antixenosis) by assessing R. maidis
physiology(survival,developmentandreproduction),andstyletpenetrationbehavior(electricalpenetration
graph) on three Miscanthus species (M. x giganteus, M siniensis and M sacchariflorus). Results brought to
lightahigherreproductionabilityofR.maidisonM.xgiganteuscomparedtothatonM.sacchariflorus;M.
sinensisnotbeingdifferentfromthe2otherplantspecies.M.sinensisexhibitedaresistanceofantixenosis
type towards R. maidis whereas M. sacchariflorus exhibited both types of resistance, i.e. antibiotic and
antixenotic. As a conclusion, this work reveals different insect resistance potentialities in the genus
Miscanthus,whichcouldbeusedaspartoftheplantvarietalselectionprocessforcropimprovement.
Keywords:Miscanthus;bioenergyculture;Rhopalosiphummaidis;resistance;expansion.
Life-history trait differentiation of Sitobion avenae populations from

both sides of the Qinling Mountains
DeguangLIU,XianliangHUANG
27
College of Plant Protection, Northwest A&F University, Yangling, Shaanxi Province 712100, China.
(dgliu@nwsuaf.edu.cn)
Sitobion avenae is a cosmopolitan cereal pest. Geographic barriers like the eastwest trending Qinling
Mountains in central China may isolate its populations and cause significant differentiation. We sampled S.
avenae populations from both north and south sides of the Qinling Mountains, and investigated their
lifehistorytraitsincommongardenexperiments.Theresultsshowedthatnorthernpopulationshadshorter
developmentaltimeforthefirstinstarthansouthernones,butlongerforthirdandfourthinstars.Thepost
reproductive time and total lifespan of adults from southern populations were longer than those from
northern populations, but no significant differences were observed for reproductive time. Southern
populationsshowedhigherlifetimeanddailyfecunditiesthannorthernones.Principalcomponentanalyses
showedthatabouttwothirdsofindividualsfromsouthernpopulationsclusteredtogethertoformagroup,
andthemajorityofindividualsfromnorthernpopulationstogetherwithsomesouthernindividualsformed
anothergroup.Althoughsignificantdifferentiationswerefoundbetweennorthernandsouthernpopulations,
southern individuals may be able to disperse northward across the Qinling Mountains. We will discuss the
roleoftheQinlingMountainsindifferentiation,localadaptationandevolutionofS.avenaepopulations.
Keywords:geographicbarrier;localadaptation;populationdivergence;wheataphid.
Comparison of Sitobion avenae life-histories on oat and barley

SuxiaGAO,XianliangHUANG,DeguangLIU
College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
(gaosx2011@nwsuaf.edu.cn)
Oat and barley are important crops worldwide, and they can also become weeds in wheat production. In
order to determine the impacts of the two hosts on population differentiation of Sitobion avenae,
lifehistoriesofdifferentclonescollectedfromwildoatandbarleywerecompared.Resultsshowedthatoat
cloneshadshorterdevelopmentaltimes,higherfecunditiesandpopulationgrowthratescomparedtobarley
clonesonoriginalhosts.Aftertransferredtobarley,theperformanceofoatclonesintermsoftotalanddaily
fecundity was decreased, but there were no changes in the developmental time of nymph. The adult
longevity of barley clones was reduced after transferred to oat while that of oat clones was increased on
barley.Agespecificsurvivalratesofoatclonestendedtobehigherthanbarleyclonesinearlieradultlifeon
originalhosts,butlowerafterthat.Thesurvivaldynamicsforbarleycloneswassimilartothatofoatclonesin
host transfer experiments. Agespecific fecundity of barley clones on barley remained low and fluctuated
significantly during their lifetime, whereas it was high for oat clones in earlier adult life, and decreased
graduallyafterthatonoat.TheresultsindicatethatthefitnessofS.avenaeclonesonoatishigherthanthat
onbarley,andoatclonesaremorespecializedtotheirhostplantthanbarleyclones.
Keywords:Sitobionavenae;hostplant;lifetable;populationdifferentiation.
Characterisation of the Acrostyle, an organ present at the tip of aphid

maxillary stylets involved in non-circulative virus transmission
MarilyneUZEST,DanielGARGANI,MalleTHILLIER,ElodiePIROLLES,StphaneBLANC
UMR BGPI, INRACIRADSUPAGRO, CIRAD TAA54/K, Campus International de Baillarguet, 34398 Montpellier
Cedex05,France.(uzest@supagro.inra.fr)
28
Countless plant viruses with major agricultural and economical impacts are transmitted by aphids in a
noncirculative manner. They are taken up in an infected plant, retained on receptors within the insect
maxillarystylets,andsubsequentlyreleasedforinoculationintonewhostplants.Thoughlargelyincomplete,
themostdetailedavailabledataontheinsectmoleculesinvolvedinnoncirculativevirusbindingarethose
obtained with Cauliflower mosaic virus (CaMV), a virus transmitted by numerous aphid species. CaMV
receptorisaproteinexclusivelyaccessibleinatinystructure,locatedinthecommonductatthetipofthe
maxillary stylets, designated the Acrostyle. The acrostyle is conserved in all aphid species tested so far
(even the nonCaMV vectors), and contains cuticular proteins with RR2 motif. Because the acrostyle
representstheonlydistinctivefeaturewithinthecommonduct,wehypothesizedthatitcouldpromotethe
spreadofmanyifnotallnoncirculativeplantviruses.Theintrinsicphysiologicalroleoftheacrostyleisstill
totally unknown. We propose that this organ could be involved in the retention and release of other
compounds, originating either from the plant (e.g. toxic compounds) or from the saliva (e.g. effectors
counteracting plant defense), and thereby play a role in aphid/plant compatibility. We are currently
developingvariousalternativeapproachestodefinepreciselytheproteincompositionandthephysiological
roleoftheacrostyle.Themostrecentadvancesonthesequestionswillbepresentedanddiscussed.
Keywords:arbovirus;insect;aphid;virus/insectinteractions;insect/plantinteractions;cuticularproteins.
New insights in mineral oil effects on Aphid Potato PVY

pathosystem
Sbastien BOQUEL1, Fadi ALDAOUD2, Jianhua ZHANG3, Manphool FAGERIA2, MarieAndre
GIGUERE3,CatherineCLARK3,YvanPELLETIER2
SIPREComitNord,RuedeschampsPotez,62217Achicourt,France;(boquel.s@gmail.com)
Agriculture&AgriFoodCanada,PotatoResearchCentre,850LincolnRd.,Fredericton,NewBrunswick,E3B4Z7,
Canada;
3
DepartmentofBiology,UniversityofNewBrunswick,Fredericton,NewBrunswick,E3B5A3,Canada.
Mineraloilhasbeenusedformorethanacenturytoreducethetransmissionofnonpersistentvirusessuch
asPotatovirusY(PVY)butitsmodeofactionisstillpoorlyunderstood.Weapproachtheproblemconsidering
theeffectofoilonthewholeAphidPotatoPVYpathosystem.Duringthe2012growingseason,wesprayed
fieldswithmineralat10Lha1(recommendeddose),5Lha1(recommendeddose)and0Lha1(Control).
The results showed that mineral oil was effective in protecting potato plants against PVY spread without
negativeeffectsonfoliagegrowth,canopyclosureandyield.AstrongeffectonPVYacquisitionbyaphidwas
highlightedandathresholdforanefficientreductionofPVYacquisitionwasdetermined.Furthermore,avery
smallamountofoiltranslocatedfromtheleaftootherplanttissues.Themineral oilstayedintheleafbut
decreased in concentration as the leaf grew, accumulation occurred in mature foliage after successive
applicationsandoilwasnotdetectedinfieldgrowntubers.Theeffectofmineraloilonthedevelopmentand
movementofPVYwasalsoevaluated.PreliminaryresultssuggestedthatthemovementofPVYisreducedin
tuberizingplantsiftheyweresprayedwithmineraloil,delayingtranslocationtothetubers.Also,mineraloil
treatedplantsreactedmorevigorouslyandfastertothepresenceofPVYbyactivatinggenesinvolvedinplant
defense.
2
Keywords:aphid;virus;cropprotection;plantdefense;translocation;phytochemicalanalysis.
Going against the grain aphid
Chris SHORTALL1, James BELL1, Steve FOSTER2, Martin WILLIAMSON2, Toby BRUCE2, Richard
HARRINGTON1
Department of AgroEcology,
(chris.shortall@rothamsted.ac.uk)
Rothamsted
Research,
Harpenden,
Herts.
AL5
2JQ,
UK;
29
2
Biochemistry&CropProtection,RothamstedResearch,Harpenden,Herts.AL52JQ,UK.
The grain aphid Sitobion avenae (Fabr.) is one of the major insect pests of cereals worldwide. Hitherto,
control using insecticides has been straightforward, though complicated to some extent by the range of
grassesthatcanactasareservoirforboththeaphidandthevirusesitcantransmit.Recentcontrolfailuresin
theUKhavebeenlinkedtoamutationconferringresistancetopyrethroids.Thecompromisingofthisclassof
insecticides,whichhasbeenthemainstayofcontrolfordecades,highlightstheimportanceofalternativeand
complementaryapproaches,twoofwhicharediscussedhere.Thefirstinvolvesadeepunderstandingofthe
mechanisms underlying population dynamics which can be applied, through modelling and through
availability of longterm data, to decision support aimed at rationalising insecticide usage. The second
involves a deep understanding of the chemical ecology of the aphid which can be applied, through
applicationofsemiochemicalsandgeneticmodificationofcropplants,todeteraphidsfromalightingonthe
crop.
Keywords: genetically modified crops; insecticide resistance; longterm data; population dynamics;
semiochemicals;Sitobionavenae.
The spatial-temporal dynamics of Aphis gossypii at a regional scale in

Northwestern China: developing forecasts for management of this
pest
ZhaozhiLU1,2,MyronP.ZALUCKI2,LyndaE.PERKINS2,PeilingWANG3,GuizhenGAO1
Key Laboratory of Biogeography and Bioresources in Arid Land, Xinjiang Institute of Ecology and Geography,
ChineseAcademyofSciences,Urumqi830011,China;(zhaozhi@ms.xjb.ac.cn)
2
SchoolofBiologicalScience,TheUniversityofQueensland,BrisbaneQLD4072,Australia;
3
CollegeofAgriculture,ShiheziUniversity,Shihezi832003,China.
The population dynamics of Aphis gossypii will influence control tactics in any cotton production system.
Limitedknowledgeofpopulationprocessaffectingcottonaphidsatalargerscalehasrestrictedtherational
control of this pest and development of better IPM. We monitored populations of this aphid in 8 counties
around the Junggar basin in northern Xinjiang, China. Aphid number was highly variable among counties
acrosstwoyears(20032004).Therewasonlyonepeakinaphidpopulations,whichoccurredinthemiddleof
growth season in northern Xinjinag. Aphid populations grew rapidly at the start of June to midJuly until
reachingapeak,andsubsequentlydeclineddramatically(acrash).Toourknowledge,thepositiverelationship
between aphid density at the peak and population growth rate (PGR) has only ever been reported in our
studyofXinjiangcottonareas.Furthermore,thepercentageofstickycottonandcurledcottonhadapositive
linearrelationshipwithaphiddensities.Theserelationshipscouldbeusedinaforecastingsystemandaspart
ofanactionthresholddecisionatacountylevelandforthelargercottonarea.
Keywords:cottonaphid;Aphisgossypii;populationdynamics;crash;stickycotton.
RegistrationAwardWinnerTalk
The development of a smart-phone support platform for monitoring

and management of aphids-barley yellow dwarf virus on small grains
in Pennsylvania
Mitzy PORRAS1, Ed RAJOTTE1, Piero CACIAGLI2, John TOOKER1, Cristina ROSA1, Joe RUSSO3,
BethGUGINO4,FrederickE.GILDOW4
30
1
Department of Entomology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA;
(mfp145@psu.edu)
2
IVVInstituteofPlantVirology,NationalResearchCouncil,Turin10135,Italy;
3
ZedXInc,Bellefonte,Pennsylvania16823,USA;
4
Buckhout laboratory, Department of Plant Pathology, The Pennsylvania State University, University Park,
Pennsylvania16802,USA.
TheaphidsRhopalosiphumpadiL.,SitobionavenaeF.,andR.maidisarethemostcommonspeciesonsmall
grains in Pennsylvania. They are key vectors of barley yellow dwarf virus (BYDV) and can cause significant
losses in some small grain cultivars. The Pennsylvania State University is enhancing the adoption of
webbased pest prediction systems to improve the management of the virus and vectors. This is a
public/privatepartnershipcouplinguniversityresearchwiththeinformationtechnologiesofaninternational
company, ZedX. Inc. ZedX developed and maintains a wide variety of crop and pest models based on
highresolutionweatherpredictions.TheseincludealfalfaweevilHyperapostica(Gyllenhal),Europeancorn
borerOstrinanubialisHuber,cornrootwormDiabroticavirgiferavirgiferaLeConte,andFusariumsp,among
others.PennsylvaniaPestInformationPlatformforExtensionandEducation(PAPIPE)providesawebbased
interfacetoinformfarmersandcropadvisorsaboutcropandpeststatusatlessthana1kmresolution.We
areintegratingsmartphonetechnologytoimproveinformationdeliverytofarmersaswellasgatheringlocal
environmental data to improve the resolution to fieldlevel and theaccuracy of model results. The system
and its application require numerous disciplines including weather forecasting, information technology,
biologicalsciencesandsociology.Thesociologicalcomponentwillassessfarmerattitudes,acrucialfactorin
technology adoption. The development of this new technology is important to maximize productivity and
minimizeenvironmentalandeconomiccostofaphidpestmanagementinsmallgrainsandothersystems.
Keywords:BYDVmanagement;aphidpopulation;pestforecasting;mobiletechnology.
Forecasting potato viruses in seed potatoes using suction traps
RolandSIGVALD
Swedish University of Agricultural Sciences, Department of Ecology, Box 7044, SE75007 Uppsala, Sweden.
(roland.sigvald@slu.se)
PotatovirusY(PVY)seemstobemoreimportantinthenorthernregionsofEuropethanpotatoleafrollvirus
(PLRV),whichcanbeexplainedbydifferencesinvectorpopulations.InthenorthernregionsofSwedenthe
spreadofPVYisminimalbecausevectorsareuncommon.Bycontrast,insouthernregionsthespreadofPVY
has led to serious problems for seed potato growers during some years. Suction traps have been used in
aphid forecasting in Sweden as well as in many countries in Europe. Suction trap catches from southern,
centralandnorthernpartsofSwedenhasbeenanalysedinrelationtopostharvesttestingofsamplesfrom
seedpotatofields.Anumberoffactorswereconsidered,suchasproportionofvirussources,plantingdate,
number of aphids, proportion of PVY infected progeny tubers and date of haulm destruction. In Sweden
strong relationship was found between suction trap catches of aphids and proportion of PVY infected
progeny tubers (r2=0.47), but even better when taking into account mature plant resistance, number of
vectors and proportion of virus sources (r2=0.78). Rhopalosiphum padi seems to be the most important
vectorofPVYinSweden.ThusbyusingsuctiontrapstoforecastPVYincidence,farmerswouldbeabletoskip
postharvesttestingduringlowdiseaseyears,therebyreducingtheiroperationalcosts.
Suction traps for monitoring aphids in China
HongbinLIANG1,QilianQIN2,GexiaQIAO1
ChaoyangDistrict,Beijing100101,China;(lianghb@ioz.ac.cn)
31
2
StateKeyLaboratoryofIntegratedPestManagement,InstituteofZoology,ChineseAcademyofSciences,Beijing
100101,China.
SuctiontrapshavebeenusedtomonitoraerialpopulationsofsmallinsectsinEuropeandNorthAmericafor
several decades. But China did not set up suction trap until recently. In a longterm project of controlling
soybeanaphidandwheataphids(20082015),leadingbyInstituteofZoology,ChineseAcademyofSciences,
suctiontrapsweredesignedandmanufactured.Thefirstninesetsofsuctiontrapwereinstalledin2009.Up
to 2012, total 32 sets of suction trap installed, mainly in Heilongjiang, Jilin, Liaoning, Hebei, and Henan
provinces.Theheightofsuctiontrapis8.8m,withsamplingairof2000cubicmetersperhour.Theyoperated
generallyfromMarchtoOctober,groupingseasonofwheatandsoybean.Theaphidsofsuctiontrapswere
samplednormallyonceaweek.Parataxonomistsateachsiteofsuctiontrapweretrainedtosortsampling
aphids at laboratory. In wheat project area, more than 60 species of small insects were caught by suction
traps.ItisclearlythatnumberofwheataphidsisverylowinsuctiontrapbeforemiddleApril,aphidpeaks
oftenappearedinlateAprilorMay.ThedateofwheataphidpeakinHebeiprovincecouldbehalfmonthlater
thanthatinHenanprovince.InHenanprovince,itisnoticedpeakofwheataphidinnorthernareaislater
than southern area, implying that migrating direction of wheat aphids is from south to north. In soybean
project,soybeanaphidsarenormallycaughtbysuctiontrapsinlateMaytoearlyJune,7to10daysearlier
than by aphid appearance on the soybean plants. Date of soybean aphid appearance in Liaoning province
couldbeoneweekearlierthanHeilongjiangprovince.Theseresultsindicateaphidnumberscaughtbysuction
trap at one place could be an alarming signal of aphid infestation both for local field and for other places
nearby. However, operation of suction trap in China is still at its infant stage, the data generated from the
suctiontrapsareexpectedmoreproperinterpretation,andmeteorologicaldatashouldbeintegratedtotrace
thelongdistancemigrationofaphidsinChina.
Keywords:suctiontraps;aerialinsects;soybeanaphid;wheataphids;populationdynamics.
Adaptation and fitness of Aphidius

Braconidae) on different aphid species
gifuensis
(Hymenoptera:
MingzhenPAN,TongxianLIU
Key Laboratory of Applied Entomology, Northwest A & F University, Yangling, Shaanxi, 712100, China.
(panmingzh@yeah.net)
Oviposition and offspring fitness of Aphidius gifuensis (Ashmead) on three aphid species, Sitobion avenae
(Fabricius), Myzus persicae(Sulzer) and Aphis gossypii Glover were investigated according to no choice test
and observation of oviposition behavior. A. gifuensis produced more mummies and adult offspring on S.
avenae and M. persicae than on Aphis gossypii. Mummy production was influenced by stab rate and
percentageofsuperparasitism.F1generationfromS.avenaeandM.persicaewasmorefemalebiasedand
largerinbodysizethanthosefromAphisgossypii.Althoughthesignificantdifferencesindevelopmenttimeof
A. gifuensis in the three aphid species were observed, they were generally shorter than one day. Fewer
mummies were produced when A. gifuensis were transferred between S. avenae and M. persicae, but no
significant difference existed in emergence rate, percentage of female in offspring, and body size of new
female adults. The effects of host species on performance of female parasitoid and offspring fitness were
discussedinthispaper.
Keywords:Sitobionavenae;Myzuspersicae;Aphisgossypii;parasitoid;hostspecies.
32
PosterAbstracts
S1.1 Silene-feeding aphids of the Macrosiphum hartigi group in
southern Europe
ShalvaBARJADZE1,RogerL.BLACKMAN2,SebastianoBARBAGALLO3
1
EntomologyandBiocontrolResearchCentre,AgriculturalUniversityofGeorgia,13thkmofDavidAghmashenebeli
Alley,Tbilisi0131,Georgia;(shalva.barjadze@yahoo.com)
2
DepartmentofLifeSciences,TheNaturalHistoryMuseum,LondonSW75BD,UK;
3
Department of Agrifood and Environmental Systems Management, University of Catania, Via Santa Sofia 100,
Catania95123,Italy.
Macrosiphum hartigi H.R.L. is so far known to live in mountainous habitats on Silene vulgaris in southern
Europe.AcloselyrelatedspecieswascollectedintheSvanetiregionofwesternGeorgiaonSilenemultifida.
Thisnewtaxon,nowunderdescriptiononthebasisofapterousandalateviviparousfemalesandalatemales,
apparentlyhasanabbreviatedlifecycle,withmalesappearinginJuly.Althoughoviparousfemaleshavenot
yetbeencollected,theshortaphidlifecycle,ifproved,maybelinkedtoanadaptiontothehostplantbiology
and/or to the severe climatic conditions of the mountainous region where it lives. Furthermore, newly
available collections of M. hartigigroup aphids from Italy show some morphological differences when
compared to the type specimens. This suggests that there should be more extensive biological and
morphologicalinvestigationsinsidethissmallgroupofSilenefeedingMacrosiphum.
Keywords:newtaxon;intraspecificvariation.
S1.2 A new method of DNA barcoding in aphids

RuiCHEN1,2,GexiaQIAO1
1
BeichenWestRoad,ChaoyangDistrict,Beijing100101,China;(chrui11@live.cn)
2
DNAbarcodingisusefulforthetaxonomicidentificationofbiologicalmaterials.Toimprovetheefficiencyof
speciesidentificationusingDNAbarcoding,weproposeanewmethodcalledtagbarcodesanddevelopa
newsoftwarepackageaphicoding1.1.Theuseoftagbarcodesisanewapproachthatinvolvesattachinga
speciesspecifictagtothestandardDNAbarcode.Withthismethod,thebarcodingoverlapcanbenearly
eliminated.Asaresult,wewereabletoincreasetheidentificationsuccessratefrom83.9%to95.2%byusing
COIandthebestclosematchtechniqueinaphids.Theaphicoding1.1packageincludesthreeparts:Mysql,
aphicoding 1.1 and dnacompare 1.0. Itcanrealize the function oftag barcodes method and improve the
efficiencyofDNAbarcodingsystem.
Keywords:efficiency;tagbarcodes;aphicoding1.1;identificationsuccessrate.
S1.3 Determination of the morphometric variations in Hyalopterus

pruni (Hemiptera: Aphidoidea) populations associated with different
host plants and localities
zhanENOL1,HayalAKYILDIRIM2,EminDEMRTA1,GaziGRR1
35
1
Nigde University, Science and Arts Faculty, Department of Biology, 51100 Nigde, Turkey;
(emindmrts@hotmail.com)
2
ArtvinCoruhUniversity,ForestryFaculty,BotanyDepartment,Artvin,Turkey.
To investigate the effects of environmental conditions on morphological features of Hyalopterus pruni

populations, morphometric analyses of ten morphological characters were carried on populations of this
speciescollectedfromdifferenthostplantsandlocations.TwowayANOVA,canonicalvariatesanalysisand
clusteranalysiswereperformedtofindoutmrophologicaldifferentiationofH.prunipopulations.Statistical
analyses indicated that while host plant has significant influence on character variation, locality has no
significant effect. Multivariate analyses indicated that morphological features of specimens collected on
Prunus persicae were significantly different from that of populations on Phragmytes sp., which was
supportedbydiscriminantanalyses.Canonicaldiscriminantanalysesrevealedthatbothbodylengthandhind
tibialengthcontributedmostintheseparationofthepopulations.Inthefuturestudy,analysesofdatafrom
moremeasurementsaregoingtogiveaclearerpatternoftheseinteractions.
Keywords:Aphididae;Hyalopterus;morphometric;Turkey.
AuthorsthanktotheTurkishScientificCouncilforfinancialysupportingourproject(111T866).
S1.4 Chaitophorinae versus Drepanosiphinae

Aphidoidea) preliminary data of molecular analysis
(Hemiptera:
KarinaWIECZOREK1,ukaszKAJTOCH2,ukaszJUNKIERT1
UniversityofSilesia,DepartmentofZoology,Bankowa9,40007Katowice,Poland;(karina.wieczorek@us.edu.pl)
InstituteofSystematicsandEvolutionofAnimals,PolishAcademyofScience,Sawkowska17St.,31016Krakow,
Poland.
Aphids belonging to the subfamilies Chaitophorinae and Drepanosiphinae (Hemiptera: Aphidoidea) are
knowntobemonoeciousandholocyclic,distributedmainlyintheHolarcticregion.About170speciesand12
genera divided into 2 bionomic groups have been described so far in the Chaitophorinae. The tribe
Chaitophorini is connected with trees (Salicaceae, Aceraceae), whereas the tribe Siphini with grasses and
sedges (Poaceae, Cyperacea). The subfamily Drepanosiphinae comprises 36 species and 5 genera, all
associated with Aceraceae. The aim of the project is the reconstruction of the phylogenetic relationship
between subfamilies Chaitophorinae and Drepanosiphinae. On the basis of morphological characters,
supportedbymoleculardata,ananalysisoftherelationshipsbetweentheseaphidswillbedone.Scenariosof
theevolutionoftheseinsectswiththeirhostplants,inrelationwithbiogeographicaldataandbasedonthe
phylogeny of the taxa, willbe analyzed and discussed at the conclusion of the project. Preliminary data of
combined molecular analysis (nuclear 18SrDNA and EF1 gene, mitochondrial COIII genes) shows close
relationswithinstudiedrepresentativesofthegeneraPeriphyllusandTrichaitophorusontheonehandand
Chaitophorus and Siphini on the other hand (Chaitophorinae). Representatives of the genera Drepanaphis,
Drepanosiphum and Yamatocallis (Drepanosiphinae) form a sister clade, with close relations among the
generaDrepanaphisandDrepanosiphum.
2
Keywords:aphid;moleculardata;phylogeneticrelationship.
ThisresearchwassupportedbytheNationalScienceCentre,Poland,grantno.2011/01/B/NZ8/00157.
S1.5 DNA barcoding of Greenideinae (Hemiptera: Aphididae) with

resolving taxonomy problems
QinghuaLIU1,2,LiyunJIANG1,GexiaQIAO1,2
36
ChaoyangDistrict,Beijing100101,China;(liuqinghua@ioz.ac.cn)
2
UniversityofChineseAcademyofSciences,Beijing100049,China.
Species of the Greenideinae subfamily are distributed mainly throughout Southeast Asia and include some
important agricultural and horticultural pests. Accurate species circumscription and identification in this
subfamily is very difficult because similar morphological traits are shared among congeneric species. DNA
barcodingisausefultoolforspeciesidentificationinadiverserangeoftaxa.Here,totesttheefficiencyof
DNAbarcodingintheGreenideinaesubfamily,weanalyzed214samplescovering42speciesbelongingto9
generausingtwomitochondrialgenefragments(COIbarcodefragmentandCytbgenefregment).Theresults
showthatDNAbarcodingisausefulspeciesidentificationmethodinthissubfamily.Bothgenescancorrectly
identify most species using NJ tree analyses and distancebased analyses. Based on the molecular and
morphological evidence, we questioned the validity of two species, Mollitrichosiphum rhusae Ghosh and
Schoutedeniaemblica(Patel&Kulkarni).FurtheranalysisofthesetwogenefragmentsshowedthatGreenidea
psidiivanderGoot,aninvasivespeciesinHawaii,ispossiblyfromChina.ThisisapreliminaryDNAbarcoding
study in Greenideinae, and comprehensive sampling is needed to test and confirm the usefulness of DNA
barcodinginthissubfamily.
Keywords:COI;Cytb;invasivespecies.
S1.6 Molecular and morphological differentiation of Lachnus pallipes

(Hrtig) and L. roboris (L.) (Aphididae, Lachninae)
EwaMRZ,JoannaTRELA,ukaszDEPA
UniversityofSilesia,Bankowa9,40007Katowice,Poland.(ewa.mroz@us.edu.pl)
ThegenusLachnusBurmeisterincludesabout14palaearcticspecies,livingmainlyonvariousrepresentatives
ofFagaceae,feedingonbranchesandtrunks.Itisadifficultgenus,composedofmanyspecieswithuncertain
host specificity and dubious morphological characteristics. In Europe, a significant example of taxonomic
difficulties with appropriate species recognition is the identity of Quercus and Fagusfeeding species: L.
roboris(L.),L.pallipes(Hrtig),L.iliciphilus(DelGuercio)andL.longirostris(Mordvilko).Someauthorsregard
L.longirostrisahostraceofL.pallipeswhileothersregardL.iliciphilusasynonymorhostraceofL.pallipes.
MorphologicalanalysisofoviparousfemalesandmalesofQuercusandFagusfeedingspecimensofLachnus
is presented, supported by analysis with molecular markers COI and COII. All specimens were collected in
PolandandSlovakiaandrepresentedmorphologicallytypicalL.roborisonQuercus,L.longirostris/iliciphilus
specimensfeedingonQuercusandL.pallipesfeedingonFagus.Asaresult,theobtainedtreerevealedthe
presence of two clades: one composed of typical L. roboris, with winged males, feeding on Quercus; the
second composed of typical L. pallipes/iliciphilus feeding on Quercus and Fagus, with apterous males,
congruent with descriptions of L. iliciphilus. As a result we suggest L. iliciphilus (and probably also L.
longirostris)asasynonymofL.pallipes,whichisoligophagousonQuercusandFagus,separatefromL.roboris,
beingprobablyoligophagousonQuercusandCastanea.
Keywords:aphidmale;hostspecificity;speciesrecognition.
S1.7 The determination of the genetic diversity of Pemphigus

spyrothecae (Hemiptera: Aphidoidea) distributed in Istanbul, Nide
and Kayseri Provinces of Turkey
HayalAKYILDIRIM1,GlayOLCABEY2,zhanENOL2,GaziGRR2
Artvin Coruh University, Orman Faculty, Forest Engineering Department, Forest Botanic, Seyitler, Artvin, 08000,
Turkey;(h.akyildirim@artvin.edu.tr)
37
2
NigdeUniversity,ScienceandArtFaculy,BiologyDepartment,Nigde,51100,Turkey.
Pemphigus spyrothecae (Hemiptera: Aphidoidea) is a major pest of Populus and widely distributed in
Turkey. In the current study, random amplified polymorphic DNA (RAPD) markers were used to
investigate the genetic diversity of P. spyrothecae samples collected in stanbul, Nide and Kayseri
provinces.Forthe14RAPDprimersused,11weresufficienttoobtaindata.Atotalof58polymorphic
bands were identified. Analyses showed that genetic differentiation (GST) between the three
populations was 0.3399 and gene flow (Nm) was 0.9708. Neis genetic variation (H) for Nide, Kayseri
and stanbul populations was 0.2542, 0.2290 and 0.2445, respectively. Results also showed that there
were about 10% genetic differences between the Nigde and Kayseri populations because of mutation
andgeneticdrift.AlthoughgeographicconditionsofNideandKayserimayhaveplayedimportantroles
ingeneticvariation,theywerenotsignificantenoughtoleadtohighergeneticdifferencesbetweenthe
two populations. However, the stanbul population showed a higher amount of variability than the
KayseriandNidepopulations.ThisisthefirstreportofgeneticdiversityofTurkeyaphidpopulations.
Keywords:aphid;RAPD;geneticdifferentiation.
S1.8 The population genetic structure of Myzus persicae in China
XiaominSU1,2,ZheWANG1,LiyunJIANG1,GexiaQIAO1
ChaoyangDistrict,Beijing100101,China;(suxiaomin@ioz.ac.cn)
2
The green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae) is an important pest in agriculture
and forestry in the world. In this study, in order to estimate the population structure of Myzus persicae in
China,a656bpregionofthemitochondrialcytochromeoxidasesubunitI(COI)geneof95individualsof34
different geographic populations were sequenced and analyzed preliminarily. Among the 34 geographic
populations,wehaddefined20haplotypesforCOIgenes,inwhichtherewere9sharedhaplotypesand11
uniquehaplotypes.TheneighborjoiningtreebasedonCOIsequencesshowedsmallgeneticdistancesamong
haplotypes. There were significant genetic differences between the southwest population group and other
populationgroups,butitshowedlowlevelofnucleotidediversityamongallthepopulations.Thesefindings
suggestedthatthepopulationstructureofMyzuspersicaemaybetheresultfromthehighlevelofgeneflow
among different populations. As there was a limit of the number of samples, we have not found any
relationshipsbetweenthegeneticdiversityandhostplants,whichwasinconsistentwithpreviousresearches
bymicrosatelliteDNAanalysis.
Keywords:aphid;Myzuspersicae;moleculardiversity;geographicaldistribution;COI.
S1.9 On the molecular systematics of genera within tribe Aphidini

(Hemiptera, Aphididae)
JurgaTURCINAVICIENE,RimantasRAKAUSKAS
Department of Zoology, Vilnius University, Ciurlionis str. 21, Vilnius LT03101, Lithuania.
(jurga.turcinaviciene@gf.vu.lt)
Phylogeneticrelationships ofgenerawithinthetribeAphidiniwereinferredfrompartialDNAsequencesof
mitochondrial cytochrome oxidase I (COI) and nuclear elongations factor 1 alpha (EF1) genes. Maximum
parsimony,maximumlikelihoodandBayesianmethodswereperformed.ThemonophylyoftribeAphidiniwas
supported. Within the subtribe Aphidina, most species represented the genus Aphis, and some genera or
38
subgenera with unresolved relationships, as Brachysiphum, Brachyunguis, Anthemidaphis, Absinthaphis

(Xerobion), Protaphis. Several species were selected as the representatives of sister tribe Macrosiphini, to
establish the placement of the genus Cryptosiphum, which has been the subject of disagreement between
authors.ThegenusPterocommawasusedasoutgroupandrepresentativeofsubfamilyPterocommatinae.In
our analysis, Brachyunguis grouped with Aphis s.str. and Protaphis clustered with Anthemidaphis and
Absinthaphis.ThegenusCryptosiphumwasplacedinMacrosiphiniasawellresolvedclade.Analysisofpartial
sequences recovered wellsupported phylogenetic relationships in the tribe Aphidini, but results would
certainlybenefitfrommorespeciesofAphidiniincludedinanalysisandfromanalysisofmoregenes.
Keywords:aphid;mitochondrialDNA;nuclearDNA;phylogeneticrelationships.
S1.10 Rapid identification of aphids on flower plants of Rosaceae in

Beijing based on DNA barcoding
JuanWEN1,2,RuiCHEN1,2,LiyunJIANG1,GexiaQIAO1
ChaoyangDistrict,Beijing100101,China;(wj890115@126.com)
2
FloweringornamentalplantsoftheRosaceaefamilyareamajorfeatureofBeijingsgardensbutarealsohost
plants for a variety of aphids. It is often difficult to identify aphids rapidly and accurately by traditional
methods due to their tiny bodies, degradation and specialization of morphological characteristics, and the
occurrenceofmultiplemorphswithinsinglespecies.WeevaluatedDNAbarcodingbasedonCOIsequencesas
ameansofrapidlyidentifyingtheaphidsfoundonRosaceaefloweringplantsinBeijing.Inthisstudy,65COI
sequences were sequenced, including 12 species from 9 genera. The average intraspecific divergence was
0.70%whereasinterspecificdivergencewas9.45%12.59%.NJ/MPtreesbasedonCOIsequencesindicated
thatmostsamplesofthesamespeciesclusteredtogetherwithstrongsupport(bootstrapvalue>90%).From
thecombination of sequence divergences and phylogenetic trees, DNA barcoding reliably identified 83% of
theaphidspecies.
Keywords:COI;divergence;floweringplant.
S2.1 Multiple mechanisms may be involved in the resistance of
Medicago truncatula to aphid Therioaphis trifolii
LinglingGAO1,LarsG.KAMPHUIS1,2,OwainEDWARDS3,KaramB.SINGH1,2
CSIROPlantIndustry,PrivateBag5,Wembley,WA6913,Australia;(lingling.gao@csiro.au)
TheUWAInstituteofAgriculture,UniversityofWesternAustralia,Crawley,WA6009,Australia;
3
CSIROEcosystemSciences,UnderwoodAve,Floreat,WA6014,Australia.
Thespottedalfalfaaphid(SAA,Therioaphistrifoliif.maculata)andspottedcloveraphid(SCA,T.trifolii)are
importantpestsofmajorpasturelegumeswithSAApreferencetofeedonalfalfa(Medicagosativa)andSCA
onredclover(Trifoliumpretense).SAAandSCAcausenotonlylocaldamage(throughdirectfeeding)butalso
systemic damage (shown as vein chlorosis in the uninfested emerging leaves) to their host plants which
representauniqueopportunitytostudyplantaphidinteractions.ThemodellegumeMedicagotruncatulaisa
suitablehostforbothSAAandSCAandthreetypesofinteractionshavebeenidentified:highlysusceptible,
moderate resistant and lethal resistant (aphids die within 2448 hours) interactions. Electronic Penetration
2
39
Graph(EPG)showedthatthetimethataphidsspentinthephloemingestion(E2)waspositivelycorrelated
withthedegreeofaphidsusceptibility.TheaphidsshowedasignificantlylongerE2phasewhenfeedingon
thesusceptibleplantthanthemoderateresistantplant.Inthelethalresistantinteraction,aphidspenetrated
epidermisandmesophyllasnormalasonthesusceptibleplantbutfailedtoreachthephloemsieveelement
astherewasnoindicationofaphidsalivation(E1)orE2phasesduring27hourEPGmonitoring.Theaphids
were able to recover and grow normally on the susceptible plant after preinfestation for 18 hours on the
resistantplant.Theresultssuggestthatlethalresistanceoperatesintheinterfacebetweenthemesophylland
phloem sieve element and aphids die from starvation rather than toxicity from the resistant plants.
Furthermore, the aphid penetration provoked early transcription activation of plant defense related genes,
particularly those involved in salicylic acid signaling pathway, which may also contribute to the lethal
resistancetobothSAAandSCA.
Keywords:sapsuckinginsect;hostresistance;plantaphidinteraction;plantdefense;electronicpenetration
graph.
S2.2 Venom transcriptomics and proteomics of Aphidius ervi,

parasitoid of the aphid model Acyrthosiphon pisum
Caroline ANSELME1,2,3, Dominique COLINET1,2,3, Emeline DELEURY1,2,3, Donato MANCINI6,

Sophie TARES1,2,3, Julie POULAIN4, Maya BELGHAZI5, Francesco PENNACCHIO6, Marylne
POIRI1,2,3,JeanLucGATTI1,2,3
INRA,UMR1355InstitutSophiaAgrobiotech(ISA),SophiaAntipolis,France;(jeanluc.gatti@sophia.inra.fr)
CentreNationaldelaRechercheScientifique(CNRS),UMR7254,SophiaAntipolis,France;
3
UniversitNiceSophiaAntipolis,Nice,France;
4
CEA,InstitutdeGnomique(IG),Gnoscope,Evry,France;
5
CNRS,Centred'AnalysesProtomiquesdeMarseille(CAPM),Marseille,France;
6
EntomologiaeZoologiaAgrariaF.Silvestri,UniversitadiNapoli,Italy.
Mostendoparasiticwaspsinjectvenomand/orovarianproductsatovipositiontopreventtheeggdestruction
bythehostimmunesystemandinduceaphysiologicalalterationofthehostthatincreasesitssuitabilityfor
theparasitoiddevelopment.Aphidiuservi(Hymenoptera,Braconidae)isanendoparasitoidof severalaphid
species,includingAcyrthosiphonpisum.Parasitizedaphidsshowdifferentdegreesofcastration,thecastration
being complete when parasitoid oviposition takes place in firstinstar hosts (Falabella et al, 2007
doi:10.1016/j.ibmb.2007.02.005.). The host castration can be reproduced by injection of a venom fraction
containing a gammaglutamyl transpeptidase (AegGT), suggesting that the observed apoptosis of host
ovarioles results from an alteration of the GSH metabolism. However, parasitoid venoms are complex
mixtures of proteins having different effects on the host physiology. To better describe A. ervi venom
components and understand their role, an indepth analysis was performed by a combined transcriptomic
and proteomic approach. Following Sanger sequencing of >5000 clones from venom glands cDNA libraries,
340 contigs and 1570 singletons were obtained and compared to databases. Proteomic analysis was
performed separately on venom glands and reservoirs by SDSPAGE and LCMSMS analysis of the main
protein bands. As expected, AegGT was the most abundant contig and protein both in the gland and
reservoirbut,surprisingly,twootherdifferentAegGTswereidentifiedintheanalysis.Interestingly,several
heatshockproteins(HSPs),serineproteases,lipasewerealsoabundantaswellasanumberofproteinswith
unknownfunction.
2
Keywords:aphid;parasitoids;Aphidiuservi;venom;transcriptomics;proteomics.
S2.3 Dpp signaling in the development of the asexual pea aphid
YiminHSIAO1,ChuncheCHANG1,2
40
1
InstituteofBiotechnology,CollegeofBioresourcesandAgriculture,NationalTaiwanUniversity,Taipei,106,Taiwan;
(skyyimin@gmail.com)
2
Department of Entomology, College of Bioresources and Agriculture, National Taiwan University, Taipei 106,
Taiwan.
The establishment of body axes in the early embryo is a longstanding problem in developmental biology.
During early embryogenesis of Drosophila melanogaster (fly), decapentaplegic (dpp) and short gastrulation
(sog) play an important role to specify DorsolVentral (DV) polarity. Dpp signaling is highly conserved in
holomometabolus insects (including D. melanogaster), but little is known about how dpp functions in
hemimetabolousinsects.InthepeaaphidAcyrthosiphonpisum,ahemimetabolousinsect,weclonedfivedpp
genes (Apdpp15) as well as one sog gene (Apsog), detecting their expressions in the pathogenic and
viviparousembryos.Duringearlyembryogenesis,wefoundthatApdpp1to5wereuniformlyexpressedinegg
chambersandApsogmRNAwasdetectedintheanteriorregionofoocytesandtheperipheryofoocytenuclei.
During gastrulation, distribution of Apdpp mRNAs remained uniform but Apsog mRNA was preferentially
expressedinonesideoftheembryos.AccordinglyweinferthatApsog,ratherthanApdpp15,isinvolvedin
DVpatterningintheasexualpeaaphid.
Keywords: decapentaplegic; short gastrulation; DorsolVentral; parthenogenetic; viviparous; Acyrthosiphon

pisum.
S2.4 Bioindication value of Pemphigus spyrothecae Passerini, 1856

(Hemiptera, Aphidoidea, Eriosomatidae)
BarbaraOSIADACZ1,RomanHAAJ2,IwonaWRZESISKA3
60594Pozna,Poland;(barbara.osiadacz@gmail.com)
2
TheUpperSilesianNatureSociety,Huberta35,40543Katowice,Poland;
3
60594Pozna,Poland.
Pemphigus spyrothecae is a holocyclic and one of few singlehost species of Pemphigus spp. and all
Pemphigini(Eriosomatidae).ItinfestsPopulusspp.,particularlyP.nigra(includingP.nigraItalica),causing
visibleandcharacteristic(clearlydifferentfromotherspecies)gallsonpetiole.Forthesereasonsthespecies
can serve as a model object of biological and ecological studies. So far studies have focused mainly on a
singleaphidcolonyaspect(e.g.theoccurrenceofparticularmorphs,theirmorphologyandabundance,social
phenomena,preypredatorcorrelations,thestructureandsizeofgallsalsoinrelationtoexternalfactorsor
hostsage).TheproblemsofthewholeP.spyrothecaepopulationinfestingahostplantoragroupofhost
plantsonparticularsiteswerestudiedonlyingeneralterms.Atthesametimeobservationsprovedthatthe
abundance of such a local population (based on the number of galls) might depend not only on climatic
conditions, but also on habitat quality, including air pollution. To verify this in 20102012 the authors
conducted studies with standardised methods. The results seem promising and may prove the value of P.
spyrothecaeasanindicatorofhabitatcondition.
Keywords:aphid;bioindicators;habitatcondition;galls;Pemphigini;Populus.
Transcriptome profiling of grain aphid (Sitobion avenae F.) alimentary

canal upon feeding and screening potential RNAi targets for aphid
control
Min ZHANG1*, Yuwen ZHOU2*, Hui WANG1, Huw JONES3,Qiang GAO2, Dahai WANG1, Youzhi
MA1,LanqinXIA1
41
1
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street,
Beijing100081,China;(xialanqin@caas.cn)
2
BeijingGenomicsInstituteShenzhen(BGIShenzhen),ChineseAcademyofSciences,Shenzhen,518083,China;
3
RothamstedResearch,Harpenden,HertfordshireAL52JQ,UK.
*
Authorscontributeequallytothework.
Grainaphid(SitobionavenaeF.)isamajoragriculturalpestwhichcausessignificantyieldlossesofwheatin
China,EuropeandNorthAmericaannually.Inthisstudy,weperformeddenovotranscriptomeassemblyand
gene expression analysis of grain aphid alimentary canal upon feeding on wheat plants using Illumina
technologyRNAsequencing.Basedonthesedata,RNAitargetswereselectedandsystemicRNAieffectwas
investigated. The transcriptome profiling generated 30,427 unigenes with an average length of 664 bp.
BLASTXagainsttheavailablepublicdatabasesrevealedthatgenesrelatedtotransportandmetabolismwere
significantlyenrichedinthealimentarycanal. Furthermore,of5490unigenesdifferentiallyexpressedupon
feeding, diverse genes and/or pathways involved were identified. Moreover, of 16 randomly selected
unigenes that was significantly up or downregulated upon feeding, dsRNAs of 5 unigenes led to higher
mortalityanddevelopmentalstuntinginartificialfeedingassayduetothedownregulationofthetargetgene
expression.Finally,byaddingthefluorescentdsRNAintotheartificialdiet,systemicRNAieffectingrainaphid
wasobserved.Theobtainedfirstgrainaphidalimentarycanaltranscriptomeprofileuponfeedingonwheat
plants provided comprehensive gene expression information that could facilitate our understanding of the
molecular mechanisms underlying feeding, ingestion and digestion. Furthermore, five novel and effective
RNAi target genes were identified and systemic RNAi effect was observed in aphid for the first time. This
findingwouldprovideafundamentalbasisforaphidcontrolinwheat.
Keywords:grainaphid;doublestrandRNA;artificialfeedingassay;RNAinterference;aphidcontrol.
S3.1 The odorant-binding protein gene family in the pea aphid,
Acyrthosiphon pisum
Jingjiang ZHOU1, Filipe G. VIEIRA2, Xiaoli HE1, Carole SMADJA3, Renhu LIU1, Julio ROZAS2,
LindaM.FIELD1
DepartmentofBiologicalChemistryandCropProtection,RothamstedResearch,Harpenden,Herts.AL52JQ,UK;
(jingjiang.zhou@rothamsted.ac.uk)
2
DepartamentdeGenetica,UniversitatofBarcelona,Av.Diagonal64508028,Barcelona,Spain;
3
DepartmentofAnimalandPlantSciences,UniversityofSheffield,SheffieldS102TN,UK.
Chemoreceptionisanessentialprocessforthesurvivalofanimals,allowingtherecognitionofvolatilecues
for the detection of food, predators and mates. Several gene families are implicated, including the
chemoreceptor superfamily, the odorantbinding proteins (OBPs) and chemosensory proteins (CSPs). OBPs
are a family of small watersoluble proteins that bind and solubilise hydrophobic odorants. They are
abundantintheaqueousfluidsurroundingolfactoryreceptorneuronsandareinvolvedinthefirststepsof
olfactorysignaltransduction.Theseproteinsarecharacterisedbyaspecificdomainthatusuallycomprisessix
helices joined by three disulfide bonds and are classified into three OBP subfamilies: Classic, PlusC and
AtypicalOBPsplustheCSPs.UsingbioinformatictoolswehaveannotatedgenesencodingputativeOBPsand
CSPsinthepeaaphidAcyrthosiphonpisum.Intotalweidentified13ClassicandtwoPlusCOBPsand13CSPs.
Wealsoobtained,byexperimentalmethods,thehomologousOBPsequencesfromnineotheraphidspecies.
Thecomparativegenomicanalysis showsthatparalogousOBPsequencesareverydivergent;nevertheless,
orthologs are quite similar among aphids and the phylogenetic relationships reflect the divergence of the
aphidspecies.Inaddition,wehavefoundafewcommonorthologousgroupsbetweenthepeaaphidandits
closest sequenced species, Pediculus humanus. Only two OBPs have clear orthologs across the insect
42
phylogeny.Overall,ourresultssupportthebirthanddeathmodelasthemajormechanismexplainingaphid
OBPsequenceevolution,withpurifyingselectionasthemainevolutionaryforce.
S3.2 Identification of lectins as virus competitors in aphid vectors:

from fundamental to applied approaches
Emilie BOSQUE1, Sophie VANDERMOTEN1, Wenjuan YU2, Rongling YIN3, Yong LIU3, Julian
CHEN2,FrdricFRANCIS1
FunctionalandevolutionaryEntomology,GemblouxAgroBioTechUniversityofLige,PassagedesDports2,
5030Gembloux,Belgium;(Emilie.bosquee@ulg.ac.be)
2
ofAgriculturalSciences,2WestYuanmingyuanRoad,Beijing100193,China;
3
CollegeofPlantProtection,ShandongAgriculturalUniversity,Taian,Shandong271018,China.
Theeffectsoflectinbindingtoglycoproteinaphidreceptorsonvirustransmissionweretestedinlaboratory
on Myzus persicae and Sitobion avenae via the use of artificial diets using different virus models. For
Canavalia ensiformis agglutinin (ConA), Pisum sativum lectin (PSL) phytohemagglutinin (PHA) and wheat
germagglutinin(WGA),differentialeffectsonvirustransmissionefficiencyweredetermined.Inconsequence,
lectinswereconsideredaspromisingproteinsandcouldbepotentialcompetitorstoviruses.Further,afast
proteinliquidchromatographywasperformedtoidentifyaphidproteinsrelatedtobindingtoefficientlectin
competitors and to detect relevant proteins in aphids, a twodimensional gel electrophoresis approach
coupled to mass spectrometry was developed to identify the glycosylated proteins determining virus
transmission efficiency. The use of lectins for both fundamental and applied approaches was discussed to
investigateaphidvirusinteractions.
Keywords:gelelectrophoresis;glycoproteins;Myzuspersicae;receptors;Sitobionavenae;virustransmission.
S3.3 Identification of intraspecies alarm pheromone and two

conserved odorant binding proteins associated with E--Farnesene
perception in Rhopalosiphum padi (Linnaeus)
JiaFAN1,JulianCHEN1,HaoYUAN2,DengfaCHENG1,JingruiSUN1,YongLIU2
State Key laboratory of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of
AgriculturalSciences,HaidianDistrict,Beijing100193,China;(jiahangyouxiang@163.com)
2
DepartementofPlantProtection,ShandongAgriculturalUniversity,Taian271018,China.
Efarnesene(EBF)isacommonactivecomponentofaphidalarmpheromone.Recently,twoorthologsof
ordorantbindingproteins(OBPs),namelyOBP3(ApisOBP3fromAcyrthosiphonpisum)andOBP7(ApisOBP7
fromA.pisumandSaveOBP7fromSitobionavenae)inaphidshavebeensuggestedtobeinvolvedinaphid
alarmpheromoneperceptionduetotheirrelativelygoodaffinitytoEBF.InsectOBPsaregroupedintoalarge
protein family with variant proteins driven by adaptive evolution. There is no homologous OBP between
invertebrates and human, and the similarity among several model insects is very poor. While aphid OBPs
shareahighsimilaritybetweenorthologswithin10species.Hereweinvestigatedtheolfactoryresponseof
Rhopalosiphum padi to EBF and fresh aphid crushed body using an olfactometer, aphids were repelled by
both EBF and crushed aphids. The released molecules from crushed R. padi were analyzed by Solid Phase
MicroExtraction(SPME)andGCMS.EBFwasidentifiedastheonlycomponentreleasedbyR.padi.cDNAsof
both OBP3 and OBP7 were isolated from R. padi and named as RpadOBP3 and RpadOBP7, respectively.
Several volatile substances including EBF were tested in binding assay with recombinant proteins of
RpadOBP3 and RpadOBP7. RpadOBP7 showed a significantly higher affinity to EBF than other molecules.
Surprisingly,RpadOBP3showednodifferenceofaffinitytoEBFtoothermoleculestested.
43
Keywords:aphid;alarmpheromone;olfaction;OBPs.
S3.4 Host plant determines the host range of cotton-melon aphid and
the tactics of the aphid to quickly adapt a novel host
XiangdongLIU,WeiWU,XinliLIANG,HaiyangZHAO,TingtingXU
DepartmentofEntomology,NanjingAgriculturalUniversity,Nanjing,210095,China.(liuxd@njau.edu.cn)
Thecottonmelonaphid,AphisgossypiiGloverisusuallyconsideredtobeapolyphagousinsectwithabout
300speciesofhostplantsin74families.However,notallthesehostplantsareutilisedequally,andcotton
andcucurbitspecializedaphidsarefoundworldwide.Thecottonspecializedaphidscannotfeedoncucumber,
and the cucurbitspecialized aphids are unable to feed on cotton. Hostplant transplantation experiments
determinedthatthezucchiniCucurbitazucchini(Cucurbitaceae)andcowpeaVignaunguiculata(Fabaceae)
are able to alter this specialization. The cottonspecialized aphids attained the ability to feed on
cucumber after they were reared on zucchini for five generations, but lost their ability to use cotton
aftersevengenerations.Thecucurbitspecializedaphidsattainedtheabilitytofeedoncottonafterthey
were reared on cowpea for one generation, but the cottonspecialized aphids still could not use
cucumber after 23 generations on cowpea. The length of femur and tibia of fore and middlelegs of
cottonspecialized aphids became longer when they were reared on zucchini for five generations. And
body size of cottonspecialized aphids became similar to the cucurbitspecialized ones after five
generations on zucchini. With each new generation on zucchini the preference of cottonspecialized
aphidsforcottondecreasedandthepreferenceforcucumberincreased.Thecottonmelonaphidsalter
theirmorphologicaltraitsandhostchoicecapacitytoquicklyadapttoanovelhostplant.
Keywords:cottonmelonaphid;hostspecialization;hostplant;ecologicaladaptation.
ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina(grantno.31070377).
S3.5 Population dynamics of Myzus persicae and its natural enemies

in the tobacco fields of Jiangxi, China
ZhaohuanXU,JianguoWANG,MeiLUO,MinYU
CollegeofAgronomy,JiangxiAgriculturalUniversity,Nanchang330045,China.(hzzhaohuan@163.com)
TobaccoisoneoftheimportantcashcropsinJiangxiProvince,China.InfestionofMyzuspersicaeontobacco
can seriously affect the yield and quality of tobacco leaves. Monitoring and forecasting is the basis for
effectivepreventionandcontrol.InordertoobtainbetterunderstandingofthepopulationdynamicsofM.
persicaeanditsnaturalenemies,weinvestigatedthenumbersofM.persicaeanditsnaturalenemiesduring
ApriltoJuly2012.TheresultsrevealedtheannualpopulationdynamicofM.persicaecouldbedescribedwith
abimodalcurve,thefirstpeakappearinginearlyJune,andthesecondpeakinearlyJuly.Naturalenemies
foundwereladybirds,lacewings,aphidparasitoids,andspiders.Thepopulationdynamicsofnaturalenemies
werecloselyrelatedtoM.persicae.Thepopulationdynamicofladybirdswasalsodescribedbybimodalcurve,
peaking~5dayslaterthantheaphids.Parasitoidshadonepeakeachyear,coincidentwiththefirstpeakof
aphidabundance;lacewingandspiderpopulationsalsohadonepeakeachyear,coincidentwiththesecond
peak.
Keywords:aphid;controllingeffect;fieldinvestigation;followingphenomenon;seasonaldynamic.
S3.6 Variation in transmission of Barley yellow dwarf virus-PAV by

44
different populations of Sitobion avenae in China
WenjuanYU1,2,ZhaohuanXU3,JulianCHEN1,ClaudeBRAGARD4,FrdricFRANCIS2,YongLIU5,
DengfaCHENG1
StateKeyLaboratoryforBiologyofPlantDiseaseandInsectPests,InstituteofPlantProtection,ChineseAcademy
ofAgriculturalSciences,Beijing,100193,China;(ywj19830906@163.com)
2
Functional and Evolutionary Entomology, Gembloux AgroBio Tech, University of Liege, Passage des Dports 2,
B5030Gembloux,Belgium;
3
CollegeofAgronomy,JiangxiAgriculturalUniversity,Nanchang,330045,China;
4
Earth and Life Institute, Applied MicrobiologyePhytopathology, Universit catholique de Louvain, Croix du Sud
2bte3,1348LouvainlaNeuve,Belgium;
5
CollegeofPlantProtection,ShandongAgriculturalUniversity,Taian,271018,China.
Fourteen populations of Sitobion avenae Fabricius originating from China were tested for their ability to
transmit Barley yellow dwarf virusPAV (BYDVPAV, one isolate from Belgium, another from China) using
wheat plants. By sequence analysis, the coat protein gene of BYDVPAVBelgium was shown to be
distinguishablefromBYDVPAVCNisolate(78%sequencesimilarity).Allaphidpopulationscouldtransmitthis
BYDVPAV and variation in transmission rates ranged from 24.422.21% to 66.673.84% with
BYDVPAVBelgium isolate and from 23.551.36% to 56.185.22% with BYDVPAVCN isolate. Significant
differences in transmission rates were observed between populations: transmission efficiency of S. avenae
fromnorthernandnorthwesternregionsofChina,whereBYDVimpactismoreimportant,washigherthan
that from the middlelower reaches of Yangtze River and Yunnan province. This work emphasizes the
importance of considering clonal diversity of aphid vectors and not only virus strain variability for BYDV
transmissionefficiencyassays.InordertomonitorthedispersionofBYDVinaparticularregion,aphidvectors
should be considered as a very important but variable factor in applying reliable epidemiologic models
worldwide.
Keywords:transmissionefficiency;Barleyyellowdwarfvirus;Sitobionavenae;RTPCR;TASELISA.
S3.7 Aphicidal activity of major alkaloids from Macleaya cordata

extract against wheat aphids
XiaoningZHANG,JulianCHEN,DengfaCHENG,JingruiSUN
State KeyLaboratoryforBiologyofPlantDiseaseandInsectPests,InstituteofPlantProtection,ChineseAcademyof
AgriculturalSciences,Beijing100193,China.(zhang.xiaoning@yahoo.com)
Wheataphidsareonesofthemostimportantpestsofcerealcrops,mainlycontrolledbychemicalpesticides
in China. The use of pesticides causes severe health and environmental problems. Botanical pesticides are
considered ecofriendly and have attracted intense attention in recent years. The insecticidal activity of
Macleayacordataextract(MCE)tomanypestshasbeenreported.However,fewstudieshaveinvestigatedits
aphicidal activity against wheat aphids, which is necessary for the development of new types of botanical
pesticides.Byusingdippingmethod,wetestedtheaphicidalactivitiesofMCEandfractionalkaloidsincluding
sanguinarinehydrochlorideandchelerythrinehydrochloride,on threewheataphidspecies,namelySitibion
avenae,SchizaphisgraminumandRhopalosiphumpadi.ResultsshowedthatMCEandfractionalkaloidshad
significant aphicidal activity against these species, with an order of chelerythrine hydrochloride > MCE >
sanguinarine hydrochloride. Their aphicidal activity increased with the increase of concentration and
treatment time. After being treated with chelerythrine hydrochloride for 48 hours at the dose of 4 g/L,
corrected mortality rates of forthinstar alate nymphs of S. avenae, R. padi and S. graminum were up to
35.96%,73.56%and60.24%,respectively;whilelethalconcentrationof50%(LC50)were11.14g/L,1.54g/L,
2.58 g/L, respectively. Artificial dietary treatments for 12 h, 24 h, 36 h, 48 h, and 72 h at the dose of 125
g/mLshowedthatsurvivalratesofalateadultsofS.avenaewere60.96%,40.78%,17.35%,3.48%,1.96%,
respectively,withthesanguinarinehydrochloride,while65.00%,49.63%,17.13%,3.61%and0%,respectively,
45
withthechelerythrinehydrochloride.
Keywords:wheataphids;Macleayacordataextract;alkaloids;aphicidalactivity;survivalrate.
S3.8 Population dynamics and demography of the soybean aphid

(Aphis glycines) and physiological responses of the soybean plants
XingyaWANG1,LihongZHOU2,XingXING3,BiaoXU3,GuoqingXU1
InstituteofPlantProtection,LiaoningAcademyofAgriculturalSciences,ShenheDistrict,Shenyang,110161,China;
(xgq66@126.com)
2
HorticulturalBranch,LiaoningAcademyofAgriculturalSciences,ShenheDistrict,Shenyang,110161,China;
3
Xiuyan Manchu Autonomous County Agricultural Technology Extension Center, Xiuyan County, Anshan, 114300,
China.
Thesoybeanaphid,AphisglycinesMatsumura(Hemiptera:Aphididae),isanimportantpestinChina.Inorder
to effectively monitor and manage this pest, it is necessary to investigate its population dynamics and
demography, as well as physiological responses of the soybean plants to its feeding. In this study, by using
fieldsurveyandsuctiontrapmonitoring,weinvestigatedpopulationdynamicsofthesoybeanaphidatXiuyan
Manchu Autonomous County, Liaoning Province in northeastern China during 20092012. The results
indicatedthatasinglepeakofsoybeanaphidsoccurredperyear:itcolonizedinsoybeanfieldsinearlyJune
and reached highest density in midJuly. Our study found that suction trap monitoring can predict the
occurrenceofsoybeanaphidsatleasttwoweeksearlierthanfieldsurvey.Atotalof47alateswerecollected
bysuctiontrapinthe4yrs,andtheearliestalatewascapturedon28Mayin2009,2011and2012,and4June
in 2010. Life table parameters showed that the soybean aphid in northeast China had a short mean
generationtime,andinaverage,aphidcountsincreased6.36timesafter13.57d.Wealsoinvestigatedthe
change of contents and activities of some enzymes in the soybean leaves under different stresses of aphid
feeding time and density. Results indicated that aphid infestation increased the activities of MDA, CAT and
PPOunderdifferentaphidfeedingtimes;whiledifferentdensitiesofaphidinfestationsignificantlyincreased
theactivitiesofSODandCAT(F2,6=42.646,P<0.05;F2,6=6.950,P<0.05).
Keywords:fecundityspecificlifetable;physiologicalresponses;populationdynamics;suctiontrap.
Role of thermo-biology in the distribution of aphids in space and time
AnthonyF.G.DIXON
Department of Biodiversity Research, Global Change Research Centre AS CR, Na sdkch 7, 37005 esk
Budjovice, Czech Republic and School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK.
(a.f.dixon@uea.ac.uk)
Distributionofaphidsinspaceandtimehasbeenmainlyviewedasreflectingthedistributionandseasonal
variationinthequalityoftheirhostplants.Likeotherectothermicorganismsaphidsareverydependenton
ambient temperature and only grow and reproduce over a relatively narrow range of temperatures, their
thermalwindow,whichiseasilymeasuredandisintheregionof20C.However,theactualrangeisnotthe
same for all species. Evidence will be presented that indicates that the ability of aphids to thrive over a
particularrangeoftemperatures,notsurprisingly,isassociatedwithwheretheyoccurintheworld.However,
whatissurprisingisthatitisalsoassociatedwith,andispossiblythemainmechanismfor,enablingseveral
speciesofaphidstocoexistonthesamespecies ofhostplant,withsomespeciesdoingwell inspringand
autumnandothersinsummer.Thisphenomenonhaslongpuzzledbothaphidologistsandecologists.
Keywords:aphids;coexistence;distributioninspaceandtime;thermalwindow;thermobiology.
46
Population dynamics of Aphis gossypii on cotton: the influence of

temperature, initial density and predator
Guizhen GAO1, Zhaozhi LU1, Ping SUN1, Lynda E. PERKINS2, Myron P. ZALUCKI2, Shengmei
ZHOU1
Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography,
ChineseAcademyofSciences,Urumqi830011,China;(zhaozhi@ms.xjb.ac.cn)
2
SchoolofBiologicalSciences,TheUniversityofQueensland,BrisbaneQLD,4072,Australia.
The population dynamics of cotton aphid (Aphis gossypii) was complicated by the extrinsic and intrinsic
factors. Here we studied the integrative effect of initial aphid density, temperature and predator on the
populationdynamicsinA.gossypiiinlaboratory.Thepresentresultshowsthatthreefactorsallplayrolesin
populationgrowingbuttemperatureisthemostimportantunderalmostallcircumstances.Temperaturehas
effectonpopulationgrowthrate(PGR)andtheasymmetry(Skewness)ofA.gossypiipositively,aphiddays,
peaknumber,peaktimingandaprobability(Kurtosis)ofA.gossypiinegatively.Theheavyinitialdensitycould
advance peak timing and boost the peak number of A. gossypii. Initial density could mediate the peaking
timingandasymmetrynegatively,buttopeakingnumberpositively.Simultaneously,predatorcouldmediate
the population growing, but regulations are variable in peak timing, peak number and aphiddays in all of
circumstance. The integrative analyses claimed that combination of three factors, or any combinations in
three factors have interactions in aphiddays, peak number and peak timing except in peak timing by the
combinationofdensitywithpredator.Moreover,GPR,whichisdecidedbythetemperaturepositively,have
thenegativeeffecttoaphiddays,peaknumberandpeaktiming.Thisstudyimpliedthattemperaturedrive
the PGR directly, and influence population dynamics indirectly. Furthermore, the management strategy of
cottonaphidwillbereconsideredundertheglobalwarmingwhichcouldaccelerateGPRoraphiddensityin
thestartingofcottonseasoninthefuture.
Keywords:Aphisgossypii;populationdynamics;populationregulation;initialdensity;aphiddays;cotton.
S4.1 Direct and indirect effects of glycoalcaloids on aphids
MounaDEBBABI1,JacquesATTOUMBR2,SylvieBALTORA1,ArnaudAMELINE1
1
FRE 3498 EDYSAN (Ecologie et Dynamique des Systmes Anthropiss), Laboratoire de BioEcologie des Insectes
PhytophagesetEntomophages,UniversitdePicardieJulesVerne,33rueStLeu,F80039AmiensCedex,France;
(arnaud.ameline@upicardie.fr)
2
Comit Nord Semences, Innovation, Protection, Recherche, Environnement (SIPRE), Rue des Champs Potez,
62217ACHICOURT,France.
Glycoalkaloidsaresecondarymetabolitesnaturallyproducedbytheplant,whichareknowntohavealarge
biologicalactivityspectrumespeciallythoseextractedfromSolanaceae.Inthiscontext,threeglycoalcaloids
(chaconine, solanine and solanidine) extracted from Solanum tuberosum (cv. Pompadour) were studied on
thepotatoaphid(Macrosipumeuphorbiae)andonthecornleafaphid(Rhopalosiphummaidis).Theirdirect
andindirecteffectswereevaluatedbystudyingrespectively(1)thefitnessofthetwoaphidspeciestopically
exposedtotheseglycoalcaloidsand(2)thehostselectionprocessofRhopalosiphummaidisonaplanttreated
by the alkaloids. The results showed that topical application had a greater effect on the potato pest (M.
euphorbiae)thanonthecornpest(R.maidis).Inaddition,plantstreatedbychaconineaffectedthefeeding
behaviourandthefitnessofR.maidis.Theseresultssuggestthatchaconineisasemipenetratingmolecule
whichcouldhaveanantibiosiseffectoninsects.
Keywords: chaconine; solanine; solanidine; Macrosiphum euphorbiae; Rhopalosiphum maidis; survival;

47
fecundity;electropenetrography;antibiosis.
S4.2 Relations between aphid development and alkaloid content of

Lupinus angustifolius
Jasmin PHILIPPI1, Edgar SCHLIEPHAKE1, HansUlrich JRGENS2, Giesela JANSEN2, Frank
ORDON1,KathleenKAUFMANN3
JuliusKuehnInstitute(JKI)FederalResearchCentreforCultivatedPlants,InstituteforResistanceResearchand
StressTolerance,ErwinBaurStr.27,D06484Quedlinburg,Germany;(edgar.schliephake@jki.bund.de)
2
Julius Kuehn Institute(JKI) FederalResearch Centre forCultivated Plants, Institute for ResistanceResearch and
StressTolerance,OTGroLsewitz,RudolfSchickPlatz3,D18190Sanitz,Germany;
3
SaatzuchtSteinachGmbH&CoKG,KlockowerStr.11,D17219Bocksee,Germany.
Lupinsareplantswithseedsrichinproteins,enhancingthesoilstructurebyitsdeeprootsandthesymbiotic
nitrogenfixingbacteriaaccumulatenitrogenintherhizosphere.Thereforelupinsasthebluenarrowedleaf
lupin Lupinus angustifolius L. are a valuable culture, especially for the organic farming and on pure, sandy
soils. Characteristic of wild lupins are the toxic alkaloids as a chemical defense against pathogens and
herbivores. Since many years breeders work to reduce the alkaloid content for breeding sweet lupins. The
reducedalkaloidcontentleadstoanincreasedsusceptibilitytodifferentpests,amongotheralsoaphids,as
indicated by own field observations. It can be expected that in case of increasing the area cultivated with
sweetlupinsandduetoclimatechangedifferentaphidspecieswillrapidlyadapttolowalkaloidlupinsand
maybecomeeconomicallyimportantpests.
Forthisaimthemultiplicationofaphidswasstudiedonasetoflupingenotypes.Followingspecieswith
Fabaceaeashostplantswereselected:
- theLupinaphidMacrosiphumalbifrons,
- theBlackbeanaphidAphisfabae,
- thePeanutaphidAphiscraccivora,
- thePeaaphidAcyrthosiphumpisum,
- theGreenpeachaphidMyzuspersicae.
Thepopulationgrowthoftheaphidswasscreenedinaclimaticchamber.After7dand14dthenumbers
of alate and apterous females and larvae on plant genotypes with low alkaloid content were significantly
higher as on bitter genotypes. The alkaloid content of the plant leaves was analyzed in freeze dried leaf
material. With exception of M. albifrons, a strong negative correlation between the concentrations of
different alkaloids and the aphid was developing. On selected genotypes the individual weight growth
development, the duration of preimaginal time and life time and population development (life table
parameters) on different temperatures was compared. Additionally the feeding behavior was studied with
theEPGmethod.
Keywords:aphidmultiplication;temperature;feedingbehavior;EPG.
S4.3 cDNA cloning of C002, a salivary gland protein in greenbug,

Schizaphis graminum
YongZHANG,JulianCHEN,JiaFAN,DengfaCHENG,JingruiSUN
State Key laboratory of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of
AgriculturalSciences,HaidianDistrict,Beijing100193,China.(sunqilong3000@hotmail.com)
C002 is a soluble protein secreted by aphid salivary gland and has been demonstrated to be essential for
aphid feeding, and may play an important role in aphidhost plant interaction. However, underlying
mechanisms are little known. In this study, the ortholog of C002 gene was indentified in salivary gland of
48
Schizaphis graminum (SgC002). The deduced amino acid sequence contains 190 amino acid residues. The
mass of the predicted mature protein is 21.6 kDa. The Nterminal signal peptide was predicted using
SignalIP4.0,withcleavagesitebetweenposition24and25residues.Interestingly,thepredictedaminoacid
sequenceofSgC002showedarelativelylowsequencesimilaritytoC002fromgreenpeachaphidM.perciae,
peapeachA.pisum,soybeanaphidA.glycines,RussianwheataphidD.noxiaandcottonaphidA.gossypii.
C002mayevolvequicklytoadapttodistinctselectivepressures,forinstance,differentdefensiveresponses
andvariablenutritionconditionsofhostplants.InsemiquantitativePCR,ahighabundanceofC002transcript
wasdetectedinsalivarygland,lowabundanceinheadsandwholebody.NoC002transcriptsweredetected
in guts or heads with salivary gland removed. We are currently focusing on C002 specific function in
aphidhost plant interaction, for instance, its influences on aphid feeding behavior, capacity of virus
transmission,anditspotentialplanttargets.
Keywords:cDNAclone;semiquantitativePCR;feedingbehavior.
S4.4 Combination of genetic resistance and management of field

borders: towards an effective and sustainable control of Aphis
gossypii outbreaks and virus epidemics in cantaloupe melon crops
Alexandra SCHOENY1, Patrick GOGNALONS1, Nathalie BOISSOT2, Pascale MISTRAL2, Virginie

CHAREYRON2,CatherineWIPFSCHEIBEL1,HervLECOQ1
INRA,UR407PathologieVgtale,Avignon,France;(alexandra.schoeny@avignon.inra.fr)
INRA,UR1052GntiqueetAmliorationdesFruitsetLgumes,Avignon,France.
Aphis gossypii is a major pest of cantaloupe melon crops, causing direct damage (sap uptake) and indirect
damage(virustransmission).ThecantaloupegeneVatconfersresistancetocolonizationbyA.gossypiiand
resistancetothenonpersistentviruses(CMV,WMV,etc)carriedbyA.gossypii.Itishoweverineffectivein
blockingthetransmissionofvirusescarriedbyotheraphidspeciesanditsuseisthusgenerallycoupledwith
aphicide treatments to limit virus transmission by noncolonizing aphids. Moreover, dueto itsintraspecific
variability, A. gossypii can adapt to various selective pressures (host plants, insecticides); this adaptation
abilityenhancestheriskofresistancebreakdown.InFrance,cantaloupeprotectionfacesadoublechallenge:
toreinforcetheeffectivenessofVatinacontextofreducingpesticideuse,andensurethesustainabilityof
VatinacontextofincreasingVatdeployment.Thehypothesistestedinthisprojectisthatanappropriate
managementoffieldborderscancontributetoregulatingthepopulationsofaphidsand/ortheirvirusload,
thereby reducing the risks of virus epidemics and resistance breakdown. Indeed, literature suggests that
flowerstripscanparticipateinpestbiologicalcontrolbyfavoringnaturalenemies(predatorsandparasitoids),
andstripsofnonhostplantscanprotectcropsfromnonpersistentvirusesbyallowingaphidstoprobeon
healthy plants and thus to lose their virus load before reaching the crops. An experimental field approach
wasinitiatedtocomparethreetypesoffieldbordermanagement(baresoil,grassstripandflowerstrip)on
theeffectivenessandsustainabilityofVat.
Keywords:aphid;Cucumismelo;integratedpestmanagement;Vat.
S4.5 Study on transmission efficiency of CMV by Myzus persicae

from different places
RonglingYIN3,4,FrdricFRANCIS1,ClaudeBRAGARD2,YongLIU3,JulianCHEN4
49
1
Unite d'Entomologie fonctionnelle et evolutive, Functional & evolutionary Entomology Universite de Liege,
GemblouxAgroBioTech,PassagedesDeportes2,5030Gembloux,Belgium;
2
Applied microbiology Phytopathology, Earth & Life Institute, Facult d'ingnierie biologique, agronomique et
environnementaleUCL,CroixduSud2,Bte3,B1348,LouvainlaNeuve,Belgium;
3
Plant protection, Shandong agricultural university, No. 61, Daizong Road, Taian, 271018, Shandong, China;
(yinrongling@163.com)
4
InstituteofPlantProtection,ChineseAcademyofAgriculturalSciences,No.2WestYuanMingYuanRoad,Beijing
100193,China.
Cucumber mosaic virus (CMV) is one of the most important viruses infected vegetables in the field, and is
transmittedbyaphidsinanonpersistentmannerinnature.MyzuspersicaecantransmitCMVeffectivelybut
the transmission efficiency is not only affected by the aphid, but also virus strains. Few studies have been
undertakenontransmissionefficiencyofgeographicalpopulationsofMyzuspersicae.Ourstudyshowedthat
individuals from Taian, Shandong turnip (STp, as follows, BJ for Beijing) transmitted D1772 virus than other
clones,andsignificantdifferencesbetweenSTpandotherclonesexpectaphidfromSTcabbage(STe)(F=5.915,
df=8, P<0.05). No significant differences were detected among other clones (F=3.226, df=5, P>0.05). For
clones on turnip from different places, STp has higher transmission efficiency of CMV than other two
populations, BJp and SJp (Jinan, Shandong). Our study indicates that aphids have more effects on virus
transmission than virus strains. Although transmission efficiency is affected by a number of factors such as
virusstrains,aphidspecies,sourceandrecipientplantspecies,aswellasaphidhostplants,vectorsinvirus
transmission are very important, which can transmit virus helpfully, especially in natural environment for
nonpersistentvirusandthecontactbetweenthemcouldbethemostimportantpartforvirustransmission.
Keywords:aphid;vegetable;nonpersisitent;effective;strain;vector.
S4.6 YDV strains in autumn sown wheat in Canterbury, New Zealand
JohnFLETCHER,MelanieDAVIDSON,AbieHORROCKS,StaceySKILL
TheNewZealandInstituteforPlant&FoodResearchLimitedPrivateBag4704,Christchurch8140,NewZealand.
(melanie.davidson@plantandfood.co.nz)
In order to determine the prevalence of the YDV strains, PAV, MAV, and RPV in autumn sown wheat, we
collectedplantsexhibitingsymptoms(redoryellowleaves),andneighbouringplantswithgreenleavesfrom
17wheatfieldsinCanterbury(NewZealand).AleafofuptonineplantswastestedindividuallyusingELISA.
PAVwasthemoreprevalentstraininplantswithredleavesandyellowleaves,andtoalesserextentinplants
withgreenleaves.Thisisincontrasttobarleyandoatplantstestedin19851986,wherethepredominant
strainswereRPVandMAV.Barleyplantstestedfromasinglelocationin1996werepredominantlyinfected
withtheMAVstrain.
Keywords:ELISA;wheat;barley;oats.
S4.7 Landscape ecology of aphids and generalist predators in an

arable and forage crops environment
MelanieDAVIDSON,DavidLOGAN,BradHOWLETT,StaceySKILL,SamanthaREAD,GarryHILL
TheNewZealandInstituteforPlant&FoodResearchLimitedPrivateBag4704,Christchurch8140,NewZealand.
(melanie.davidson@plantandfood.co.nz)
Theinfluenceoflandscapefeaturesontherelativeabundanceanddistributionofaphidsandtheirkeynatural
enemiesarebeingexploredinastudyundertakeninandaroundarableandforagecropsinmidCanterbury,
NewZealand.SixtyeightsiteswereestablishedbetweenthecoastandtheSouthernAlpsinmidCanterbury.
50
Twocylindricalyellowstickytrapsweresetupateachsite;oneattheedgeofaforagekalecropandasecond
approximately 50 m into the crop. The traps were left in the field for two weeks in late spring and a
subsequenttwoweeksinmidsummer.Thenumbersofallaphidsandarangeofnaturalenemies(Micromus
tasmaniae,Coccinellaundecimpunctata,Melangynanovaezelandiae,Melanostomafasciatum)wererecorded.
Wearecurrentlyexploringtheinfluenceoflandscapefeaturesincludingtopographicalfeaturessuchasrivers,
altitude,andperennialvegetationusinggeographicalinformationsystems.
Keywords:agroecosystems;brownlacewing;ladybirds;syrphids;foragekale.
S4.8 Density estimation of ground-dwelling predators in wheat fields

in Northwest China
JunheLIU1,MingfuYU1,ZihuaZHAO2
Department of biological Engineering of Huanghuai University, Zhumadian, Henan 463000, China;

(liujunhe79@126.com)
2
State Key CAS Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese
AcademyofSciences,Beijing100101,China.
High abundance and diversity of natural enemies is the basis for biological pest control in wheat fields.
However,populationdensitiesofgrounddwellingpredatorsarepoorlyknown,despiteitspotentialtoreduce
cerealaphidpopulations.Incurrentstudy,grounddwellingpredatorsweresampledweekly,unlessweather
conditionsprecludedsampling,fromsixgeographicallyseparatedwheatplantingregionsduring20092012
in Ningxia Hui Autonomous Regions in Northwest China. Dvac suction combined with hand collection was
employed to collect samples. Cereal aphids were also sampled. Results showed that density of
grounddwelling predators ranged from 5.13 to 8.27 individuals per square meter depending on locations.
However, population density of grounddwelling predators was not related to the density of cereal aphids,
whichindicatesgrounddwellingpredatorsdonotexhibitanumericalresponsetocerealaphids.Ninespecies
ofgrounddwellingpredatorsaccountedfor78%ofallgrounddwellingpredatorscollected.Communitiesof
groundingdwellingpredatorsvariedinspeciescompositionanddensityamongthesixwheatfields.Chlaenius
palliesandPterostichusgeblerweredominantspeciesatalllocations.Densityofgrounddwellingpredators
wasvariableamongdifferentlocations,butrelativelyhigherthanEurope.Theseresultsbringintoquestion
theextenttobiologicalcontrolofcerealaphidsbyusinggrounddwellingpredatorsinwheatfields.
Keywords:biologicalcontrol;speciesdiversity;cerealaphids.
S4.9 Effects of within-field position and surrounding habitats on the

community of the natural enemies of cereal aphids in wheat field
ZihuaZHAO1,3,CangHUI2,YingshuZHAO1,RuipingAN1,DahanHE1
AgriculturalSchools,NingxiaUniversity,Yinchuan750021,China;(zhaozihua@ioz.ac.cn)
Centre for Invasion Biology, Department of Botany and Zoology, Stellenbosch University, Matieland 7602, South
Africa;
3
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese
AcademyofSciences,Beijing100101,China.
2
Spatial structure of agricultural landscapes can have a strong impact on the distribution and diversity of
insects.Herewestudiedtheeffectsofwithinfieldpositionofwheatfieldsaswellassurroundinghabitatson
thecommunitystructureofthenaturalenemiesofcerealaphids.Twelveagriculturalsiteswereincludedin
thestudy,eachwithtwospringwheatfieldsselected(oneadjacenttoanalfalfafieldandtheotheradjacent
to a corn field). We sampled two rows per field (1 or 20 m from the edge) using pitfall trapping for
grounddwellingpredator,netsweepingforleafdwellingpredatorsandhandcollectingofaphidmummiesfor
51
parasitoididentification.Theabundanceanddiversityofthenaturalenemieswerefoundsignificantlyhigher
neartheedgesthaninthecentersoffieldsadjacenttoalfalfaareas.Incontrast,nosignificantdifferencewas
foundbetweenedgesandcentersoffieldsadjacenttocornfields.Ambientalfalfaareas,asopposedtocorn
fields,cansignificantlyincreasetheabundanceanddiversityofleafdwellingpredatorandparasitoidnearthe
field edges. Four of the fifteen most abundant species, Aphidius avenae (Haliday), Aphidius gifuensis
(Ashmead), Hippodamia variegata (Goeze) and Chrysopa sinica (Tjeder) were significantly more abundant
near the edge than in the center. Being adjacent to alfalfa habitats could enhance parasitism and
predator/preyratioofleafdwellingpredatorattheedges,buthasnoeffectongrounddwellingpredator.In
conclusion,itshouldbeconsideredfordesigningefficientbiologicalcontrol,thattheresponseofthenatural
enemiesofagriculturalpeststowithinfieldpositionandsurroundinghabitatswasspeciesspecific.
Keywords:abundance;alfalfa;edgeeffects;parasitoid;speciesdiversity.
S4.10 Rice aphid diversity, occurrence and prevention in Pingxiang
HanglianGONG1,ChaohuiGONG2,MenglinLI1
1
2
PlantProtectionStation,Pingxiang,Jiangxi337000,China;(ghl1942916@sina.com)
AgriculturalTechnologyPopularizesStation,Pingxiang,Jiangxi337000,China.
The rice aphids in China have been reported by Zhang Guangxue, including 10 species in 5 genera and 2
families, as Sitobion avenae (Fabrlcius), Schizaphis graminum (Rondani), Rhopalosiphum maidis (Fitch),
Rhopalosiphum padi (Linnaeus), Rhopalosiphum nymphaeae (Linnaeus), Rhopalosiphum rufiabdominale
(Sasaki), Hyalopterus amygdali (Blanchard), Tetraneura akinire Sasaki, Macrosiphum sp., and one species in
Drepanosiphidae.Thenumbersofriceaphidspeciesfoundindomesticwereaddedto13,sixmorethanthat
recorded on Chinese Agricultural Pests Directory. An investigation on feeding indicated that the rice aphid
occurred1014generationsayearinPingxiangarea,5or6generationsinearlyriceand79generationsin
latericewithsomeoverlappedgenerations.Theinteriorleaveswereharmedattheseedlingstage,whilethe
ricestalkswereharmedatthepaniclestage.Theexcretedhoneydeweasilyleadstosootymould,affecting
the photosynthesis and accelerating the aging of the rice. The analysis of sampling survey showed the rice
aphid had a poisson distribution in rice, and its occurrence was linearly proportional to an increase in the
level of N application. Artificial inoculation with 8, 15, 20, 50 aphids per panicle, the 1000grain weight
decreased 10.92%, 15.78%, 17.97%, 27.76%, respectively. Through pesticide screening, appeared better in
riceaphidcontrol.
Keywords:riceaphid;species;occurrence;prevention;Pingxiang.
S4.11 Studies on the external morphology comparisons between

winged and wingless of Sitobion avenae (Fabricius) (Hemiptera:
Aphididae)
FangmeiZHANG,XiangruiLI,YunhuiZHANG,DengfaCHENG
Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of
AgriculturalSciences,HaidianDistrict,Beijing100193,China.(Fangmeizh@tom.com)
Wingscontributetotherangeexpansionsofphloemfeedingaphids.Becauseofthemorphologicalplasticity
in wings, aphids became a classic model species in studying insect wing development. Here, a microscopic
analysis revealed the detailed morphological descriptions of the wing development of the grain aphid,
Sitobionavenae(Fabricius)(Hemiptera:Aphididae),oneoftheworldwidedevastatingpestsofwheatcrops,
forthefirsttimebyusingthescanningelectronmicroscopy.Thedevelopmentof S.avenaeconsistsoffive
instars.Therewerenoobviousdifferencesbetweenthe1stand2ndinstarsofbothwingedandwinglessaphids
inexternalmorphologyexceptforthebodysize.Thesmallwingpadswereobservedinthe3rdwingedinstars
52
inthemesothorax.Withthegrowthofthenymphs,thewingsdevelopedanelaboratemetamorphosisuntil
thefinalformationofwingedadult.Inwingedaphid,thediameterofmesothoraxinthe3rdinstarswaswider
than that of the 2nd instars and extended gradually in the 4th instars. In wingless aphid, no wing pad was
identifiedinthe3rdand4thinstarsandtheadultswerediscriminatedfromthe4thinstarsonlybythelengthof
cauda.TheresultsindicatedthatthedivergencebetweenwingedandwinglessS.avenaeoccurredinthe3rd
instars.However,thequestionthatthewingdimorphismmechanismoccuredin2ndor3rdinstarsneededto
befurtherstudiedcombiningthehistologicalandultrastructuralevidencesinwingdevelopment.
Keywords:Sitobionavenae;wingdevelopment;nymph;scanningelectronmicroscopy.
S4.12 Mineral oil penetration and translocation in potato plant
SbastienBOQUEL1,MarieAndreGIGUERE2,YvanPELLETIER3,JeanPierrePRIVE4
SIPREComitNord,RuedeschampsPotez,62217Achicourt,France;(boquel.s@gmail.com)
DepartmentofBiology,UniversityofNewBrunswick,Fredericton,NewBrunswick,E3B5A3,Canada;
3
Agriculture&AgriFoodCanada,PotatoResearchCentre,850LincolnRd.,Fredericton,NewBrunswick,E3B4Z7,
Canada;
4
Agriculture&AgriFoodCanada,SenatorHervJ.Michaudresearchfarm,1045St.JosephRd.,Bouctouche,New
Brunswick,E4S2J2,Canada.
Mineraloilhasbeenusedformorethanacenturyinmanycropstoreducethetransmissionofnonpersistent
virusessuchasPotatovirusY(PVY)butsofaritsmodeofactionispoorlyunderstood.Thecurrenthypothesis
isthatmineraloilreducesacquisitionandtransmissionofnonpersistentvirusesbyaphidvectorsbycreating
a nonstick coating on the aphid stylets that prevent the virus being carried to or released in neighboring
plants.Bymakingthemineraloilflorescentwithalipophilicdye,theabilityofmineraloiltopenetratethe
plantanddiffusewithintheplanttissueswasexamined.Resultsshowedthatlessthan20minwereenough
foroiltopenetrateplanttissues.Mineraloilwasdilutedortranslocatedwithinplanttissuesforseveraldays
afterapplication.OurresultsconstituteessentialinformationfortheoptimizationofPVYcontrolsystemfor
thepotatoseedproduction.
2
Keywords:spray;kinetic;penetration;coloration.
S4.13 Mutualistic interaction between ants and aphids through the

honeydew: bacterial composition and diversity in the honeydew of
Cinara tujafilina (del Guercio)
CongWEI1,HongyanWANG2,HongHE2
1
2
CollegeofPlantProtection,NorthwestA&FUniversity,Yangling,Shaanxi712100,China;(congwei@nwsuaf.edu.cn)
CollegeofForestry,NorthwestA&FUniversity,Yangling,Shaanxi712100,China.
Aphids are commonly found having mutualistic interactions with ants. Their honeydew can be a significant
foodresourceforsomeants.CandidatusSerratiasymbiotica,asecondaryendosymbiontofaphids,hasbeen
reported also existing in the gut of ant Camponotus japonicus Mayr, whose foraging workers were often
observedfeedingonthehoneydewofaphidCinaratujafilina(delGuercio).Recently,werevealedmutualistic
interactionbetweenCi.tujafilinaandCa.japonicusinmidJuneof2011,i.e.foragingworkersoftheanttend
the crowded nymphal aphids in special nests on the aphids host plant, the Oriental cypress Platycladus
orientalis(L.)Franco.Thenestsweremadebytheantstoguesttheircowsbybitingofffibersofphloem
andxylemofthetreetrunks.However,nonymphalaphidswerefoundfromtreetrunksyetinJulyof2011;
theywereallmovedtothetwigsbyants,andthelatterwerestillobservedfeedingonthehoneydew.Thus,
we investigated the bacterial diversity in the honeydew collected in July of 2011 using both the culture
method and 16S rRNARFLP analysis. In total 20 bacterial strains were isolated with the culture method,
53
which belong to seven genera of Bacteroidetes and Proteobacteria. Thirtyfour distinct restriction profiles
werefoundbythe16SrRNARFLPanalysis,whichrepresent14knownbacterialspeciesandthreeunknown
species. The Maximum Likelihood tree clusters all clones into five groups, i.e. Betaproteobacteria,
Gammaproteobacteria, Alphaproteobacteria, Firmicutes and Verrucomicrobia. Among which Pseudomonas
aeruginosawasthemostdominantspecies,accountingfor54%inclonelibrary.However,CandidatusSerratia
symbioticawasnotdetected.Consideringthemicrobiotainthehoneydewcouldbeinfluencedbynotonly
the aphids but also the host plants and the ambient environment, the further studies on the bacterial
diversity in the honeydew, particularly those collected in the other months, are merited to address the
presenceandtransmissionoftheendosymbiont.
Keywords:endosymbiont;microbiota;mutualism;Orientalcypress;transmission.
S4.14 Development and application of suction trap, an instrument to

monitor the occurrence of aphids and other migratory insects
QilianQIN1,HongbinLIANG2,LinMIAO1,HuanZHANG1,GexiaQIAO2
StateKeyLaboratoryofIntegratedPestManagement,InstituteofZoology,ChineseAcademyofSciences,Beijing
100101,China;(Qinql@ioz.ac.cn)
2
ChaoyangDistrict,Beijing100101,China.
ReferringtotheprincipleanddesignoftheRothamosted12msuctiontrap,wedevelopeda8.8msuction
trapandproduceditformorethan30byauthorizingamanufacturerHenanJiyuanBaiyunIndustryCo.,Ltd.
All the setup suction traps have established a suction trap network throughout China. Since its initiate
establishment in 2008, the network has been used to monitor the aerial population dynamic of the wheat
aphids and the soybean aphid Aphis glycines. Main body of the 8.8m suction trap is built from fiber
reinforcedplastic,whichislighterandmorewearproofthanpolyvinylchloride(PVC),themainbuildingstuff
oftheRohamostedsuctiontrap.Themainparametersoftheinstrumentincludethatthesuckedairperhour
is2000m3,thepowerofthefanis0.12kwwith1450rpmrotationspeedandelectricalsourceistheChinese
normalpowersupplyof50Hz,220V.Thewholebodyofthesuctiontrapweighs200kgwithsetupheight8.8
meters.Wedesignedatimeswitchtoautomaticallycontroltheinstrumentonandoff,whichfacilitatesthe
trapoperatemoreefficientaccordingtothepopulationdynamicsofthetargetaphidsinvariousgeographical
locations. Up to date, 32 8.8m suction traps were set up distributed in Beijing city and the provinces of
Helongjiang, Jilin, Liaoning, Hebei, Shandong, Henan, Jiangsu, Shaanxi and Guangxi in China and some
monitordatawereobtainedfromthenetwork.
Keywords:suctiontrap;soybeanaphid;Aphisglycines;wheataphids;monitor.
54
Authorindex
A
Akyildirim,Hayal
12,35,37
AlDaoud,Fadi
29
Ali,Abid
18
Ameixa,OlgaM.C.C.
18
Ameline,Arnaud
27,47
An,Ruiping
51
Anderson,Jonathan
25
Anselme,Caroline
40
Asanidze,Zezva
8
Attoumbr,Jacques
47
B
Baltora,Sylvie
Ban,Liping
Barbagallo,Sebastiano
Barjadze,Shalva
Belghazi,Maya
Bell,James
Blackman,RogerL.
Blanc,Stphane
Boissot,Nathalie
Boquel,Sbastien
Bosque,Emilie
Bragard,Claude
Bruce,Toby
47
23
35
8,35
40
29
35
28
26,49
29,53
43
45,49
29
C
Caciagli,Piero
30
Cardenas,Leyla
16
Chakrabarti,Samiran
7
Chang,Chunche
13,40
Chareyron,Virginie
49
Chen,Jing
6
Chen,Julian 25,43,45,48,49
Chen,Rui
4,11,35,39
Chen,Ying
8
Cheng,Dengfa
43,45,48,52
Chung,Chenyo
13
Clark,Catherine
29
5
CoeurdAcier,Armelle
Colinet,Dominique
40
Couty,Aude
27
D
Davidson,Melanie
DeClerck,Caroline
Debbabi,Mouna
Deleury,Emeline
Demirta,Emin
Deng,Mingming
Depa,ukasz
Desneux,Nicolas
Dixon,AnthonyF.G.
50
11
47
40
12,35
20
20,37
17,18
46
Du,Chao
Du,Erxia
20
20
E
Edwards,Owain
25,39
F
Fageria,Manphool
29
Fan,Jia
25,43,48
Fang,Yan
8
Favret,Colin
4
Field,LindaM.
42
Figueroa,ChristianC.
16
Fletcher,John
50
Foster,Steve
29
Francis,Frdric11, 13, 43, 45,
49
FuentesContreras,Eduardo 16
G
Gao,Guizhen
30,47
Gao,Huanhuan
20,24
Gao,Lingling
25,39
Gao,Qiang
41
Gao,Suxia
28
Gargani,Daniel
28
Gatti,JeanLuc
9,40
Ge,Feng
23
Giguere,MarieAndre 29,53
Gildow,FrederickE.
30
Giordano,Rosanna
5
Gognalons,Patrick
49
Gong,Chaohui
52
Gong,Hanglian
52
12,35,37
Grr,Gazi
Gratiashvili,Nana
8
Gugino,Beth
30
Guo,Huijuan
23
Guo,Jiangli
8
Guo,Sumin
25
H
Haaj,Roman
Harrington,Richard
Haubruge,Eric
He,Dahan
He,Hong
He,Xiaoli
He,Yi
Heimbach,Udo
Hill,Garry
Horrocks,Abie
Howlett,Brad
Hsiao,Yimin
7,41
29
13
51
53
42
25
26
50
50
50
13,40
Hu,Xiangshun
20,22,26
Hu,Zuqing
20,22,24,26
Huang,Xianliang
27,28
Huang,Xiaolei
10,14
Hui,Cang
51
Hull,Maurice
21
J
Jaguenet,Euriel
27
Jansen,Giesela
48
Jaquiery,Julie
3
Jiang,Liyun 4,6,11,36,38,39
Jijakli,Haissam
11
Jones,Huw
41
Jousselin,Emmanuelle
14
Junkiert,ukasz
6,36
Jrgens,HansUlrich
48
K
Kajtoch,ukasz
Kamphuis,LarsG.
Kaufmann,Kathleen
Kindlmann,Pavel
6,36
25,39
48
18
L
Lagos,DorisM.
5
Lavandero,Blas
19
Lecoq,Herv
49
Lei,Fumin
4
Lepoivre,Philippe
11
Li,Kai
8
Li,Menglin
52
Li,Xiangrui
52
Li,Xingyi
4,5,10
Li,Xuejun
3
Liang,Hongbin
31,54
Liang,Xinli
44
Lichtensveig,Judith
25
Lin,Geeway
13
Lin,Mingder
13
Liu,Aihong
19
Liu,Chunlai
3
Liu,Deguang
27,28
Liu,Junhe
51
Liu,Lin
4,10
Liu,Qinghua
4,36
Liu,Renhu
42
Liu,Tongxian
22,32
Liu,Xiangdong
44
Liu,Xiaofeng
22
Liu,Yong
43,45,49
Logan,David
50
Lu,Hsiaoling
13
Lu,Yanhui
17,18
55
Lu,Zhaozhi
Luo,Kun
Luo,Mei
30,47
26
44
M
Ma,Youzhi
Mancini,Donato
Massart,Sbastien
Mehrparvar,Mohsen
Mei,Lu
Messelink,GerbenJ.
Miao,Lin
Mistral,Pascale
Mkdk,Piyaratne
Mrz,Ewa
25,41
40
11
21
8
18
54
49
12
20,37
N
Nouhaud,Pierre
O
Olcabey,Glay
Ordon,Frank
Ortz,Sebastin
Osiadacz,Barbara
37
48
19
7,41
P
Pan,Mingzhen
Peccoud,Jean
Pelletier,Yvan
Pennacchio,Francesco
Perkins,LyndaE.
Philippi,Jasmin
Piffaretti,Josphine
Pirolles,Elodie
Pointeau,Sophie
Poiri,Marylne
Porras,Mitzy
Poulain,Julie
Prive,JeanPierre
Prfer,Tony
32
3
29,53
40
30,47
48
14
28
27
10,40
30
40
53
15
Q
Qiao,Gexia3,4,5,6,10,11,14,
31,35,36,38,39,54
Qin,Qilian
31,54
Qu,Cheng
19
R
Rajotte,Ed
Rakauskas,Rimantas
Rambaud,Caroline
Ramrez,ClaudioC.
Read,Samantha
Rosa,Cristina
Rozas,Julio
Russo,Joe
56
30
22,38
27
19
50
30
42
30
S
SalvadorRecatal,Vicenta 15
Schliephake,Edgar
48
Schmitz,Antonin
9
Schoeny,Alexandra
49
enol,zhan
12,35,37
Shortall,Chris
29
Sigvald,Roland
31
Silva,AndreaX.
16
Simon,JeanChristophe
3,9
Singh,KaramB.
25,39
Skill,Stacey
50
Smadja,Carole
42
Su,Xiaomin
11,38
43,45,48
Sun,Jingrui
Sun,Ping
47
Sun,Yinpeng
23
Sun,Yucheng
23
T
Tares,Sophie
40
Thieme,Thomas
15,24,26
Thillier,Malle
28
Thomas,Sophie
26
Tjallingii,W.Freddy
15
Tooker,John
30
Trela,Joanna
37
Tsuchida,Tsutomu
11
Turcinaviciene,Jurga
38
U
Uzest,Marilyne
28
V
Vandermoten,Sophie
13,43
VanlerbergheMasutti,Flavie14,
26
Vieira,FilipeG.
42
Villalba,Joris
9
Voegtlin,David
5
Vorburger,Christoph
9
W
Wang,Chunping
Wang,Dahai
Wang,Genping
Wang,Hongyan
Wang,Hui
Wang,Jianguo
Wang,Peiling
Wang,Szuchieh
Wang,Xingya
Wang,Yuan
Wang,Zhe
Wei,Cong
Weisser,WolfgangW.
26
41
25
53
25,41
44
30
13
46
4,14
11,38
53
21
Wen,Juan
4,11,39
Wieczorek,Karina
6,36
Williamson,Martin
29
WipfScheibel,Catherine
49
Wrzesiska,Iwona
41
Wu,Kongming
17,18
Wu,Wei
44
X
Xi,Yuqiang
Xia,Lanqin
Xing,Xing
Xu,Biao
Xu,Guoqing
Xu,Tingting
Xu,Zhaohuan
Xue,Ming
3
25,41
46
46
46
44
44,45
19
Y
Yang,Jie
Yin,Rongling
Yu,Fang
Yu,Min
Yu,Mingfu
Yu,Wenjuan
Yu,Xiudao
Yuan,Hao
24
43,49
3
44
51
43,45
25
43
Z
Zalucki,MyronP.
30,47
Zaremba,Audrius
22
Zhang,Fangmei
52
Zhang,Huan
54
Zhang,Jianhua
29
Zhang,Li
24
Zhang,Min
25,41
Zhang,Ruiling
4
Zhang,Shangan
23
Zhang,Xiaoning
45
Zhang,Xu
3
Zhang,Yanzhou
3
Zhang,Yong
48
Zhang,Yongjun
25
Zhang,Yunhui
52
Zhao,Haiyang
44
Zhao,Huiyan 12,20,22,24,26
Zhao,Yingshu
51
Zhao,Zihua
51
Zhou,Dong
24
Zhou,Jingjiang
16,42
Zhou,Lihong
46
Zhou,Qingsong
3
Zhou,Shengmei
47
Zhou,Yuwen
41
Zulak,Katherine
25
The9ISAOrganizingCommitteewouldliketothankthehardpreparatoryworkofmanypeople,including
thesessionchairsandthesevolunteers:
JingChen,JuanWen,BinZhang,XiaominSu,ZheWang,YuanWang,QinghuaLiu,LinLiu,RuiChen
ProgramandAbstractsBookEditors:
XiaoleiHuang,JingChen

9th International Symposium On Aphids (Abstract Book)

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9ISA 2013

Saturday, 1 June 2013; Hotel Lobby

Sunday, 2 June 2013; 7th Floor Ballroom

Lin LIU, Li X, Huang X, Qiao G Evolutionary relationships between Pemphigini

10:20 Caroline DE CLERCK, TSUCHIDA T, MASSART S, LEPOIVRE P, FRANCIS F, JIJAKLI H

Tuesday, 4 June 2013

Wednesday, 5 June 2013; 7th Floor Ballroom

Sophie THOMAS, VANLERBERGHEMASUTTI F, BOISSOT N Effects of genetic

Shalva BARJADZE, BLACKMAN RL, BARBAGALLO S Silenefeeding aphids of the

ENOL , AKYILDIRIM H, Emin DEMRTA, GRR G Determination of the

Lingling GAO, KAMPHUIS LG, EDWARDS O, SINGH KB Multiple mechanisms may be

ANSELME C, COLINET D, DELEURY E, MANCINI D, TARES S, POULAIN J, BELGHAZI M,

Barbara OSIADACZ, HAAJ R, WRZESISKA I Bioindication value of Pemphigus

Jingjiang ZHOU, VIEIRA FG, HE X, SMADJA C, LIU R, ROZAS J, FIELD LM The

BOSQUE E, Sophie VANDERMOTEN, YU W, YIN R, LIU Y, CHEN J, FRANCIS F

Wenjuan YU, XU Z, CHEN J, BRAGARD C, FRANCIS F, LIU Y, CHENG D Variation in

PHILIPPI J, Edgar SCHLIEPHAKE, JRGENS HU, JANSEN G, ORDON F, KAUFMANN K

Alexandra SCHOENY, GOGNALONS P, BOISSOT N, MISTRAL P, CHAREYRON V,

Melanie DAVIDSON, LOGAN D, HOWLETT B, SKILL S, READ S, HILL G Landscape

Zihua ZHAO, HUI C, ZHAO Y, AN R, HE D Effects of withinfield position and

Hanglian GONG, GONG C, LI M Rice aphid diversity, occurrence and prevention in

Fangmei ZHANG, LI X, ZHANG Y, CHENG D Studies on the external morphology

Qilian QIN, LIANG H, MIAO L, ZHANG H, QIAO G Development and application of

Evolutionary genetics and mechanisms of plant specialization and

DNA barcoding of Hymenoptera parasitoids reared from soybean

Screening and development of DNA barcoding in aphids

Cybertaxonomy to accelerate aphid systematics and collaboration

Session Keynote Talk

Contribution to the systematics of the genus Aphis (Hemiptera:

Department of Entomology, University of Illinois at UrbanaChampaign, Illinois, Urbana 61801, USA;

Molecular phylogeny of the Pemphigini (Hemiptera: Aphididae:

Siphini Mordvilko, 1928 (Hemiptera: Aphidoidea: Chaitophorinae) a

A total-evidence phylogenetic analysis of Hormaphidinae (Hemiptera:

Session Keynote Talk

Why are aphids in xerothermic habitats so unique?

Diversity and distribution of Eriosomatinae (Aphididae: Hemiptera)

The effect of urbanization on aphid diversity in different areas of

Morphometric investigation of the grapevine and willow root feeding

The evolutionary ecology of symbiont-conferred resistance to

The cellular immunity of the pea aphid model Acyrthosiphon pisum

Antonin SCHMITZ1,2,3, Joris VILLALBA1,2,3, JeanChristophe SIMON4, JeanLuc GATTI1,2,3,

Evolutionary relationships between Pemphigini (Hemiptera: Aphididae:

Identification of bacterial symbionts in the banana aphid (Pentalonia

Wolbachia diversity and

Catastrophic behavior and aphid ecology: an analysis of swallowtail

The determination of the genetic variations in Cinara cedri (Hemiptera:

Session Keynote Talk

Germline genes and germline development in the pea aphid

Recent advances on aphid alarm pheromone biosynthetic pathway

Phylogeography, life cycle evolution and differentiation of sibling

Comparative analysis of mitochondrial genomes of five aphid species

Session Keynote Talk

Phloem punctures by aphid stylets

AutoEPG analysis of the electrically monitored feeding behaviour of

Aphid olfaction and their potential in pest management

Insecticide resistance of the aphid Myzus persicae (Sulzer) in Chile

Bt cotton adoption promotes biocontrol services in agricultural

Effects of different natural enemies on population dynamics of wheat

Nonlinearities lead to qualitative differences in population dynamics of

The interspecific competition of Bemisia tabaci B biotype and Myzus

Defensive behavior of aphids against parasitoids exhibiting host

Polyphagism of Stomaphis wojciechowskii Depa (Aphididae,

Life table evaluation of survival and reproduction of the aphid,