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concise communication
m e th o d s
We conducted a laboratory-based experimental study at the
University of Geneva Hospitals, using 15 healthy HCWs from the
infection control program with extensive training and expertise
in hand hygiene. In the experiment, hand hygiene action was
completed under the close supervision of 2 senior infection
control experts. HCWs had their hand surface area calculated by
standard methods and classied as small (375 cm2), medium
(376424 cm2), or large (425 cm2).8
We used the reference strain Escherichia coli ATCC 10536
grown according to the European Norm 1500 standard
(EN 1500)9 to obtain a homogeneous bacterial suspension
containing from 2.0 108 to 2.0 109 colony-forming units/mL.
resul ts
Four participants had small hands (mean [SD] hand surface
area, 332.9 [22.2] cm2), 6 had medium hands (404.2 [9.7]
cm2), and 5 had large hands (473.2 [40.4] cm2). Overall, the
mean (SD) level of contamination of hands at baseline was 6.2
(0.58) log10 and there was no difference between the hand size
categories (P = .372).
Figure 1A shows the interquartile E. coli log10 reduction
observed according to the volume of ABHR applied. The mean
reduction of bacterial count was 0.28 log10 for each additional
increase of 0.5 mL of ABHR (95% CI, 0.200.36, P < .001).
Bacterial reduction was inversely and signicantly associated
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d i s c u s s io n
Signicant efforts have been made globally to improve hand
hygiene compliance in healthcare.2,5 At this time, however,
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http://dx.doi.org/10.1017/ice.2015.271
a ck n ow le d g m e n t s
We acknowledge all the HCWs who participated as volunteers in the present
study, and Ross Leach for his substantial editing contribution to the manuscript.
Financial support. Infection Control Programme, University of Geneva
Hospitals and Faculty of Medicine, Geneva, Switzerland.
Potential conicts of interest. All authors report no conicts of interest
relevant to this article.
Afliations: 1. Infection Control Programme and World Health Organization Collaborating Centre on Patient Safety (Infection Control & Improving
Practices), University of Geneva Hospitals and Faculty of Medicine, Geneva,
Switzerland; 2. Social Medicine Department, Ribeiro Preto Medical School,
University of So Paulo, Ribeiro Preto, So Paulo, Brazil.
Address correspondence to Didier Pittet, MD, MS, Infection Control
Programme and WHO Collaborating Centre on Patient Safety, University of
Geneva Hospitals, 4 Rue, Gabrielle-Perret-Gentil, 1205, Geneva, Switzerland
(didier.pittet@hcuge.ch).
Presented in part: Third International Conference on Prevention and
Infection Control; Geneva, Switzerland; June 18, 2015 (Abstracts 302 and 303).
Received July 29, 2015; accepted September 14, 2015; electronically
published November 24, 2015
2015 by The Society for Healthcare Epidemiology of America. All rights
reserved. 0899-823X/2016/3702-0016. DOI: 10.1017/ice.2015.271
221
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http://dx.doi.org/10.1017/ice.2015.271