Beruflich Dokumente
Kultur Dokumente
2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
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findings, leaving important gaps in our knowledge. Thus, more structured, longitudinal studies
are warranted. To address these gaps, this study
assessed the relationships between ECC incidences
and several behavioral risk factors (dietary and
oral hygiene practices), demographic risk indicators and birth-related factors in a high caries-risk
population.
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0.15 from the bivariate analyses. A manual backward elimination procedure was performed to
choose the best model. Changes in Akaike information criterion (AIC) were not considered when
evaluating the fitted model generated from the preceding step, because AIC is sensitive to changes in
sample size associated with deleting variables from
the models (15). Thus, the variables remaining in
the multivariable models were determined using
only P-values. Independent variables that had the
highest P-values were eliminated sequentially, so
our final model had variables with statistically significant parameter estimators at P 0.05.
For the incidence of ECC (16) as a dependent
variable, we had twelve possible incidence dependent variables (the six each with dichotomous outcomes and count outcomes). However, four total
dependent variables over two incidence periods
were assessed: incidence from age 1 to 4 (dichotomous and count) and incidence from age 2 to 3
(dichotomous and count). Incidence from age 1 to
4 (n = 73) was chosen because it allows study of
the full 3-year period; for incidence from age 2 to 3,
the sample size is slightly larger (n = 83), with high
incidence of ECC, so it afforded greater power to
detect differences in incidence with different dietary and oral hygiene practices.
As all children received fluoride varnish semiannually except three who missed only one application, fluoride varnish application was not
included in the bivariate or multivariable analyses.
Results
Detailed results of caries prevalence and incidence
were reported elsewhere (16). At age 1, 1.1% had
caries prevalence (mean dmfs = 0.02), increasing to
65.8% (mean dmfs = 11.51) at age 4. Caries incidence was 65.8% (mean dmfs = 11.51) from age 1 to
4. Behavioral and clinical questionnaires were completed by the majority of parents (99%). Birthrelated information collected at baseline showed
that approximately 12% of the children were not
full term (3742 weeks), 22% had low birthweight,
and 46% were delivered by C-section. Baseline feeding practices collected at baseline showed that
almost all of the children were never breast-fed
(97%) and approximately 28% had a history of night
or naptime bottle-feeding. The percentages of using
well water, bottled water, and city water were 2%,
75%, and 37%, respectively, with parents checking
more than one source of water, if applicable.
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Table 1. Dietary and oral hygiene characteristics of participants at age 1, age 2, and age 3 years
Age 1
Age 2
Age 3
Category
Variables
Percentage
(yes)
Percentage
(yes)
Percentage
(yes)
Foods
NAa
NAa
94
96
96
96
93
87
83
95
95
NAa
NAa
97.9
91.7
99.0
1.0
40.5
13.6
32.5
2.1
2.1
86
79
86
86
81
81
86
81
86
86
66
14.8
100
94.3
71.2
98.8
80.5
96.3
45.5
97.4
14.8
57.6
79
79
81
81
81
81
81
81
81
76
73
37.2
100
94.7
73.4
97.9
100
100
78.2
100
50.6
64.1
Beverages
Oral Hygiene
Dental Care
Table 2. Mean frequencies of dietary consumption and toothbrushing behaviors per day
Category
Foods
Beverages
Oral hygiene
Daily frequency
consumption of
Age 1 (n = 96)
Mean (SD)
Age 2 (n = 86)
Mean (SD)
Age 3 (n = 81)a
Mean (SD)
Area-under-the-curve
(AUC)b (n = 81)c
Mean (SD)
NAd
NAd
3.89 (1.31)
2.44 (1.00)
2.40 (1.31)
0.03 (0.31)
0.41 (0.50)
0.13 (0.37)
1.64 (0.37)
3.39 (1.20)
2.14 (0.96)
3.94 (1.12)
2.23 (1.39)
1.75 (0.51)
0.35 (0.58)
1.76 (0.53)
3.25 (1.30)
1.99 (1.39)
4.65 (1.09)
0.69 (1.23)
1.94 (0.24)
0.15 (0.26)
1.65 (0.46)
3.36 (1.00)
0.88 (0.63)
4.09 (0.89)
1.88 (0.90)
1.77 (0.35)
Sample size at second follow-up was 81, except for daily frequency of consumption of candy and/or gum and sweetened food (both = 79) and daily frequency of toothbrushing (n = 80).
b
AUC covered the period from age 1.5 to 3.0 years.
c
Sample size for AUC was 81, except for daily frequency of consumption of candy and/or gum and daily frequency of
toothbrushing (both = 78) and daily frequency of consumption of sweetened foods (n = 77).
d
NA = Not applicable, as questions were not asked at baseline.
e
Sweetened foods include Pop TartsTM, sugared cereals, etc.
f
Milk included infant formula at baseline.
g
Sugar-added beverages include all beverages with added sugar. This excludes milk, 100% juice, and sugar-free drinks.
sweetened food (95% CI 2.3236.67, Pvalue = 0.002). Children who were delivered prematurely had approximately 80% lower odds of
developing 3-year incidence of ECC, compared to
those who were delivered full term (95% CI 0.04
0.99, P-value = 0.049). Also, children who consumed 100% juice (AUC) one more time per day
had approximately 60% lower odds of developing
dental caries for the 3-year incidence of ECC, compared to those who consumed 100% juice
(AUC) less frequently per day (95% CI 0.130.99,
P-value = 0.049). Two-way interactions were not
significant between different combinations of the
variables (all P-values > 0.10). After adjustment for
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B.
Dichotomous
dependent variable
Three-year incidence
from age 1 to age 4
(n = 67)
Resultsa
Multivariable logistic analysisb
Variables in the final modelf
c
Area-under-the-curve (AUC) of
daily frequency of sweetened
foods consumptiond
Premature delivery (<37 weeks)
AUCc of daily frequency of 100%
juice consumption
Daily frequency of toothbrushing
at age 1
History of previous visit to a
dentist at age 3
OR (95% CI)
P-value
9.22 (2.3236.67)
0.002
0.21 (0.040.99)
0.37 (0.130.99)
0.049
0.049
0.34 (0.150.75)
0.01
4.57 (1.1418.25)
0.03
Multivariable negative
binomial model analysisb
Model
C.
D.
Count dependent
variable
Variables in
the final model
Estimate (SE)
Incidence rate
ratios (IRR)e
P-value
Three-year increment
from age 1 to age 4
(dmfs increment,
n = 60)
Increment from age 2
to age 3 (dmfs
increment, n = 63)
No variables
significant at
P < 0.05
NA
NA
NA
Presence of a
regular dentist
by age 3
1.29 (0.61)
3.63
0.03
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Discussion
Early childhood caries is most common among
specific racial and ethnic groups; therefore, this
report investigated ECC by assessing caries among
a cohort of African American children at baseline
and then following them for 3 years. The children
in our cohort lived in what is termed the Black
Belt counties in Alabama, with the black in the term
Black Belt referring to the color of the soil, which is
very rich and fertile. Although these counties are
very rich in their agrarian resources, the people
who live there are, as a group, among the poorest
people in the United States (17). A strength of our
study was that detailed demographic, dietary, and
oral hygiene information was collected longitudinally at the recruitment and follow-up visits. This
detailed information at different stages during the
childrens development helped us assess the relationships between incidence of ECC and different
dietary and oral hygiene behaviors over time.
Our study showed a negative association
between greater daily frequency of consumption of
100% juice and 3-year ECC incidence. This might
be due in part to the negative correlation between
daily frequency of consumption of 100% juice and
daily frequency of consumption of sugar-added
beverages (Spearman correlation = 0.60 with Pvalue < 0.0001). Sugar-added beverages most commonly have sucrose or high-fructose corn sirup,
while 100% juices contain fructose and glucose
without sucrose (18). Cariogenic bacteria, such as
S. mutans, are more effective in metabolizing
sucrose (present in sugar-added beverages), rather
than fructose or glucose, to produce extracellular
glycan (1921). Extracellular glycan enables acids
diffusion, which increases the risk for developing
dental caries (1921). Our finding regarding the
negative association between greater consumption
of 100% juice and ECC incidence was consistent
with data reported by Chankanka et al. (22)
showed that greater frequency of 100% juice was
significantly associated with fewer noncavitated
and cavitated caries surfaces (P = 0.003 and
<0.0001, respectively).
Marshall et al. (18) reported that, when using
caries (cavitated and noncavitated combined) as a
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report, leaving room for misinterpretation of questions and social desirability bias; however, study
coordinators assisted parents in completing questionnaires, answering any clarifying questions they
had; (iv) the study is a secondary data analysis of
previously collected data, which limits our analysis
to variables available, and we could not assess the
fluoride content of toothpastes used or whether the
parents used water to dilute juices; (v) due to the
absence of a control group, the effect of fluoride
varnish applications could not be determined, but
the regular application of fluoride varnish among
study subjects may have affected the results and
therefore limited comparisons to other studies
where fluoride varnish was not provided; and (vi)
the data about the consumption of sugar-added
beverages and 100% juice were based on caregivers report which could be associated with
social desirability bias (27).
Overall, the findings of our study showed that
ECC incidence in African American children was
significantly associated with greater frequency of
sweetened food consumption, lower frequency of
100% juice consumption, and lower frequency of
toothbrushing.
Conclusion
This study provides a longitudinal assessment of
the relationships between ECC incidence and different behavioral factors in African American children to the dental literature. Both greater
frequency of sweetened foods consumption and
reporting a previous visit to a dentist were associated with higher incidence of dental caries, while
greater frequency of toothbrushing and greater frequency of 100% juice consumption were associated
with lower caries incidence. Due to lack of generalizability, additional large-scale studies evaluating
risk factors for caries development among other
risk groups are necessary to better understand the
disease process and determine foci for prevention
of caries.
References
1. Drury TF, Horowitz AM, Ismail AI, Maertens MP,
Rozier RG, Selwitz RH. Diagnosing and reporting
early childhood caries for research purposes: a report
of a workshop sponsored by the National Institute of
Dental and Craniofacial Research, the Health
Resources and Services Administration, and the
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2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
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