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Inheritance of the Neurotoxinft-N-Oxalyl-L-Ct, t-Diaminopropionic cid (ODAP)in Grass pea
 (Lathyrus sativus
 L.) Seeds.
A thesisSubmitted to the Faculty ofGraduate Studiesby
Khusi Ram Tiwari
In Partial Fulfilment of theEequirement for the degreeof
Master of Science
Department of Plant ScienceUniversity of ManitobaWinnipeg, Canada(c) June, 1994
 
THE UNIVERSITY OF MANITOBAFACULTY OF GRADUATE STUDIESThe undersigned certify that they have read a Master's thesis entitled:
INHERITANCE OF THE NEUROTOXIN/3-N-OXALYL-Ur, /S-DIAMINOPROPIONIC ACID ODAP OR BOAA)IN GRASS PEA
 Lathyrus sativus
 L) SEEDSsubmitted by
 KHUSI RAM TIWARI
in partial fulfillment of the requirements for the degree ofMASTER OF SCIENCEThe Thesis Examining Committee certifies that the thesis (and the oral examination, ifrequired) is:Approved |
 ~/~\ .
 Not approvedDate: June 24, 1994
 
ABSTRACT
Khusi Ram Tiwari, MSc. The University of Manitoba. Inheritanceof the Neurotoxin fi-N-Qxalvl-L-g, £- Diaminopropionic Acid(ODAP) in Grass pea
 (Lathyrus sativus
 L.) Seeds. MajorProfessor, Dr. Clayton G. Campbell.Grass pea
 (Lathyrus sativus
 L.) is an important food, feed andfodder legume drought resistant crop. A strong epidemiologicalassociation is known to exist between consumption of grass peaand lathyrism. A neurotoxin, fi-N-Oxalyl-L-a,£-diaminopropionicacid (ODAP) has been identified to be the causative principle.This study was undertaken to investigate the mode ofinheritance of the neurotoxin, a prerequisite for thedevelopment of neurotoxin free or low neurotoxin lines. Fivegrass pea lines with low to high ODAP concentration wereinter-crossed in all possible combinations (both crosses and
reciprocals).
 Parents, F
x
 and F
2
 progenies were evaluated underfield condition and ODAP analyzed by ortho-phthalaldehydespectrophotometric method. Many of the progenies of low x lowODAP crosses were found to be low in ODAP concentration whichindicated that the low ODAP lines shared some genes in commonfor seed ODAP content. However, wider variation of ODAP in F
2
progenies as compared to parental and F
x
 progenies, suggestedthe presence of different modifier genes between lines. The F
x
progenies of the low ODAP x high ODAP crosses wereintermediate in ODAP concentration which indicated lack of

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