Disclosure to Promote the Right To Information

Whereas the Parliament of India has set out to provide a practical regime of right to
information for citizens to secure access to information under the control of public authorities,
in order to promote transparency and accountability in the working of every public authority,
and whereas the attached publication of the Bureau of Indian Standards is of particular interest
to the public, particularly disadvantaged communities and those engaged in the pursuit of
education and knowledge, the attached public safety standard is made available to promote the
timely dissemination of this information in an accurate manner to the public.
1 +, 1 +

01 ' 5

The Right to Information, The Right to Live

Step Out From the Old to the New

Mazdoor Kisan Shakti Sangathan

Jawaharlal Nehru

IS 873 (1974): Liquid Glucose [FAD 2: Sugar Industry]

! $ ' +-
Satyanarayan Gangaram Pitroda

Invent a New India Using Knowledge

! > 0 B

BharthariNtiatakam

Knowledge is such a treasure which cannot be stolen

IS : 873 1874

Indian Standard

REAFFIRMED
xxx
2005
2010

SPECIFICATION FOR

LIQUID GLUCOSE

( First Revision)
Fourth Reprint JANUARY 2005
( Incorporating Amendment No. I
and Including Amendment No.2)

UDC 664.162-404

C Copyright1983

BUREAU OF INDIAN STANDARDS

MANAK BHAVAN. 9 BAHADURSHAHZAFARMARG
NEW DELHI 110002

Gr6

October 1974

AMENDMENT NO. 3 JUNE 2011

TO
IS 873 : 1974 SPECIFICATION FOR
LIQUID GLUCOSE
(First Revision)
[Page 6, clause 5.2(f)] Substitute Net quantity for Net mass.
[Page 6, clause 5.2(h) (see also Amendment No. 2)] Substitute the following for the existing:
Any other requirements as given under the Standards of Weights and Measures (Packaged
Commodities) Rules, 1977 and the Prevention of Food Adulteration Act, 1955 and the Rules framed
thereunder.
[Page 6, clause 5.2.1, line 9] Substitute Net quantity for Net mass.

(FAD 2)
Reprography Unit, BIS, New Delhi, India

Indian Standard
SPECIFICATION FOR
LIQUID GLUCOSB

(First Revision J
Edible Starches and Glucose Sectional Committee, AFDC 33
.",.,."",
or Health Servicea ( MiDlatry of
Health It FamilyPlumial ), New Delbl

Directorate General

DB S.

Bo..M'-'"

Natioaal Supr lnatitute ( MInistry

( Department 01 PoocI), ICaDpur

0' ApicWture )

DB A. N. SalVAftAYA ( MIawII)

OeDtral Committee lor Food StaDdarda

SIUQ D. S. CHADHA

(~01

Health. Family PIaDDiDI ).. New DeIhl

Sa n..1 Mt1KBDJD (MImM,.)

V. P. CIIADJIA
Bbarat Starcb & Chemicals Limited. New DeIbi
SIIBI N. n, S'.OBAL ( AI"",." )
CJWaIU.
Technical StandardiaatioD Committee (YIaIatIy 01
A,ricuIture ). New Deihl

SDI

SJIOaftABY ( AI",." )
SJIJU CBDt7BB.. J MAlO....1

AU

SUI B. K. SABDAKA
SIDU H. Il. S. IYDGAB

Starch

Manwaeturen

AIIociatioa,

(~I,,",,*)

IDcIJaD CoDreetloDery MaauCaeturera' AMociadoa,

New Delhi

8DJ L. N. MA'IB1JB ( ~ )

SOl .R.. S. Ina

India
Bombay

lacIJaD

Chemical
Caleutta

Manufacturers'

~_

SBBJ O. K. NAJa ( ~,.." )

SJIaIll. V. JVXAUAB
ADD Starch Product. Ltd, Ahmed.bad
SOlO. N. PA.IEB ~ MIIrMII )
DB 0. R.. KaJ.....oo.HY
Tapioca Market EqluaiOD Board, ~ 01
KeraIa, TriftDdrum
SUI s. K.. L~K'BJ01IdA~A.A
CeDtral'ood TecbDoJocical RaearcIl Iaad,,*
Myae
Da H. S. R. Da,UOBA. ( ~
SUI S. K. MnD.,_
.....
Supr r... Ltd, Ravalpoa
Da R. S. Dv.JIY ( ~ )
( e",,;.,w ,.,. 2 )

c.;:::).

.. Copyright J983
BUREAU OF INDIAN STANDARDS

" . publica" II . . . . . . UDder

~~.ID wboll ~ '" My
.......... be...... to be

",.. ~ . . ( XIV .f lIS') ...

...,' Wlda
tIM
__t or ."......
die ... AcI.

wriU::;-_."

JI.I7I . -

("""'~Jr-""

I )

M.IIn
SJmI A. S.

".",..,

IluADIIYAUB

Ctom Producta Co ( Inelia ) Private Limited, Bombay

Simi S. ll. PALRAB ( MImwU )

SOl v. IluEVlld
. Kamala Supr MilIa Ltd, Udamalpet
SOl N. N. K. Ph.AD ( M....,. )

SHBI S.IUIU.WAIIY

DIrectorate Geperal or Techaical

New Delhi

Development,

8. .1 S. N. lUo
Du Company, Bombay
S-m M. N. HAD ( M....,. )
SBIU M. G. SA'nIJI
Pederatlon orBilcuit Manufacturen or India. Delhi
S"IR.D.~WAua(~. . . )

0. P. M. SIUB
Maize ProclUetl, Ahmedabad
8. .1 V. C. PAUL ( AI...." )
CoL K. N. SSJU.
~eraauterGeneral'. Branch, Army Headquarters,
New Delhi
L-r-CoL O. P. K.~ ( ~;t""'" )
DB HABI Buow._,
Director Galeral, lSI ( &-qffiM MIffIb,,)
Director ( AlI'i. Food )

s.r.-;,
SD! G. S. VILKJIV
Deputy Director ( Api Food ). lSI

AMENDMENT NO. 2

NOVEMBER 1995

TO
IS 873: 1974 SPECIFICATION FOR LIQUID GLUCOSE
( Fint Revision)

( Page 3, clause 0.1, last sentence ) -

Substitute the following for tbe

existing:

'It may be manufactured by acid or enzyme hydrolysis of stllCh. The source of

starcb materials generaOy used include tapioca, corn and rice.'

( Page 3, claus O.s, line 3 ) - Add 'and the Standards of Weights and
Measures ( Pac1ctlged Commodities) Rules, 1977' after the word 'thereunder'.
( Page 4, clause Z.I, line 4 ) 'hydrolysis'

Add 'acid er enzyme' before tbe wold

(Page 4, clause 4.1, line 2 ) - Substitute the following for the existing:
'It shall be clear, free from Cennentation, mouldy growth, sediment, dirt or other
suspended and extraneous matter, or added sweetening or flavouring agents or
any other deleterious substances.'

(Page S, Table 1) - Substitute the following for the existing:

TABLE 1 REQUIREMENTS FOR UQUID GLUCOSE (ALL GRADD)

(Clause 4.4)
SL No. CJwlAcrEIusnc

REounu!MENT

M811IOD OFTEsT, REP 10

(1)

(2)

(3)

i)

TotallOlicIs, percent
by maa.Mu.

80

AD_x C of Ibis lII.clard

0.3

5 otIS 4706 (Part 2): 1978-

ii)

Ash, pereent by IIWI, Jla

iii)

pH

iv)

Sulphur cloxide,

mlftl, JltIJt

4.8 to 5.5
40
400( For

(4)

13 of IS 4706 (Part 2 ) : 1978I10f IS 4706 ( Plrt 2) : 1978-

lupr coDfectioDery )

v)

Ane.ie, mAt MtIJl

13 of IS 6287 : 1985t

vi)

Copper. "JIkI. Ma

15of IS 6287 : 1985t

vii)

Lead, mAt Ma

14 of IS 6287: 1985t

Amend No.1 to IS 873 : 1'74

TABLE 1 ( Coaduclecl)
SL No. CIlARACl'KRIS'nC

REQUIREMENT

MITHODO.TIsT,RI.1'0

(Q
(~
viii) EscherdtitJ Coli, in 20 I

Absellt

IS 5887 ( Pan 1) : 1976*

ix)

AbseIl1

IS 5887 ( Part 3) : 19761

SlllmOMI"'. in 20 I

~)

Q)

-Methodsof tesl foredible slarchMaDd starchproducts: Part2 Cbemi<21 methods(first revisio" ).

fMelhods ofsampliDIaDd analysis for suaarconfectioDery (pnt revisio,,).
*Methodsof detectioD of bacteriaresponsible for food poilODiaa: Part llsolatioD, identificatioD and
eDumeration of &e1NrdtitJ Coli (fin' IWisioII ).

f Methods of detectionof bacteriaJeSpouibie for food poilOaiaa : Part 3 IlOlAtioD aDd idealificatioD
of S,I1Ift011eIl. aDd $JUgel'" (fint revisio,,).

(Page 6, clause 5.2(c) ] - Substitutethe followingfor the existing:

'c) Nameand addJess of manufacturer,'.

( Page6, clause 5.1 ) - Add the followingIt the end:

'g) Date of expiry;

b) Any other details required under the Stdndards

Measures ( Packaged Commodities) Rules, 1977.'

01

Weights and

( Page6, clause 5.1.2 ) - Substitute the followingfor the existing:

'5.3 DIS Certification Marking
The product may also be marked with the SgDd.rd Mark.
NOlB - n. use of lb. Standard M.rk is aowmed by the provisioDi of abe Bureau of
IDdian Sian." Act, 1986aDd the Rules aDd Rep1aliou made thereuDeler. 11le .lails of
conditions uDder which licence for tbe use of Stadard Mark may be lranced to
manufacturers or proce.oll may be obtaiDeeI fro..... Bureau of IDdia.StaDdarcls.

( Page 6, clause 7.2, line 2 ) - Substitute 'IS 107$) : 1992' lor 'IS : 1070
1960'.
( Page 6, foot-note with c.' ,,",rk ) existing tide:

Substitute the following for the

-ReapDI pde wlter( dUrtl rMsitM ).'

( Page 7, clause A.2.1, line 2 ) Conversion grade material'.

Delete the words cof the Regular

(FAD2)
Printed at N.. lDclia Prifttina Pr_, Khurja, Indi.

18 1173 117t

Indian Standard
SPECIFICATION FOR
LIQUID GLUCOSE

( First Revision)
o.

FOREWORD

0.1 This Indian Standard (First Revision) was adopted by the Indian
Standards Institution on 11 March 1974, after the draft finalized by the
Edible Starches and Glucose Sectional Committee had been approved by
the Agricultural and Food Products Division Council.
0.2 Liquid glucose is extensively used in confectionery, biscuit and food
canning industries. To a limited extent, it also finds use in textile, leather
and paper industries. It is, at present, manufactured in the country by
the C acid hydrolysis' of starch.
0.3 This standard was first published in 1956.

The various flovisions of

that standard had been under the review of the Sectiona Committee
responsible for the preparation of this standard, and the present revision
based on the experience gained both by manufacturer s and users, is being
issued. This revision incorporates a number of important modifications,
namely, (a) number of grades has been increased from three to five incorporating additional grades of regi..d ar and extra high conversions; (b) the
requirements of total solids, sulphated ash, iron, zinc and tin have been
deleted; (c) the additional requirements of total solids, dextrose equivalent
(DE:' value have been included; and (d) the limit for ash has been
lowered.
0.4 Thia stendard stipulates the use of a 2 54-em cell in Lovibond Tintometcr
for determining the colour of liquid glucose. While realizing that
measuring colour in lO-cm and 15-cm cells would make colour
determination more accurate and precise, the Sectional Committee, for
want of data, decided not to make any change in the Indian Standard at
this juncture. However, the Committee recommended that comparative
data using different sues of cells in determining colour of liquid glucose
should be collected, and if in the light of these results it was found
necessary, the Indian Standard should be modified.
0.5 In the P' eparation of this standard due consideration has been given
to the Prevention of Food Adulteration Act, 1954 and the Rules framed
thereunder. However, this standard is subject to the restrictions impos~~
under these, wherever applicable.

111175 1174

0.1 For the purpose ordeci~ whether a particular r~emeDt ofthil

standard is complied with, ,the fiDal value, obaerved or calculated, expreaing the result of a teat or analysis, ahall be rounded off in accordance with
IS : 21960. The number of lignificant places retaiDed in the rounded off
value should be the same as that of the specified value in thia 1taDdard.

1. SCOPB
1.1 Thi. standard paescribea the requirements and the methods ofsampling
and teat Cor liquid glucose.

2- TERMINOLOGY
2.1 For the purpose of this Itandard. liquid glucose or glucose syrup .haD
mean a refined and concentrated non-erystalJizable aCJueoul solution or
d-glucose, maltose and other polymen of d-glucolc, obtamed by controDed
hydrolysis of starch containing material.

S. GRADEl
3.1 The material shall be of the following five lI'ad~:

a) Low convenion (LC),

b)
c)
d)
e)

Regular conversion (RC),

Intermediate conversion ( Ie J,
High conversion ~ He), and
Extra high conversion ( EHe).

4. IlEQ,UIRBMBNTI
4.1 The material shall be in the form of an odourleaa and vilcou ayrup
with a characteristic sweet taste. It shall be clear, tree trom rermentatioD; mouldy growth, sediment, dirt or other IUlpended and extraneoul
matter, or added sweetening and flavouring agentle

t.2 Colo... - When a 50 percent (_/,,) solution oftbe material of regular

converaion grade is tested in a Lovibond Tintometer in a 2'M-em cell by
the method prescribed in Appendix A, the colour of the material in terms
of Lovibond units shall not be deeper than:
a) 0-1 yellow and 01 red within a period of 90 daya from the date
of manufacture, and
b) O~ yellow and 0'1 red within a period of ISO days from the date
of manufacture.
-aulel lor round. . off numericalvalues ( "",.).
4

111173 lI7t

U Wheal deten;Dined accordin, to the method prescribed in Ap~ B,

the datrole equivalent (DE) value for difFerent graclea ahaIl be as
follOWl:
Low CODVcrUOD ( LC )
28 to 37
R.egular conversion (RC)
38 to 47
Intermediate conversion ( LC )
High conversion ( HC )
Extra high CODvenion (EHC )

48 to 57
38 to 67
68 and abovr

t.t The metarial shall also comply with the requirements given in
TabJe 1.
TAIILB 1 RBQ,tJlRDlBNTS .OR UQ.UID GLOCOSE ( ALL GRADEl )
8L

No.

~U'ftalftlO

1lIIQ'OIIUIIDft

r-

MB'BOD 01' T. . . 1lIII' -.ro

,J..

Appendix

QNo.o(

IS : 4706-1968(S)

(4)

i) TotaiIOUda, perceat by
. . . . Mia

80

II) Alb..

0-1

( I)

(2)

perceDt

Ma

by ....,

,H

Iii)
iY) Sulphur diaDde. ppm,

.
10

4-8 to 55
400

Ala
v) Aneaic, ppm, Ma
vi) Copper, ppm, Ma
YIl) Lead, ppm. Ma

1-0
5
2

(5)

8
D
B
p

Metboda or tat for edible stuchel.

5. PACKING AND MARKING

5.1 PacIdDl- The material ahall be packed in dry and leak-proof

containen. Unless otherwise a~ between the purchaser and the
vendor. such containen may be either lacquered or lined steel druma, or
made of tin plats.
5.2 MarklDI - The container. shall be suitably marked witl- the
roUowing information:
4&) Liquid glucose.
b) Grade of the material.

II-m 1174
c)
d)
e)
f)

Name of the manufacturer,

Date of manufacture,
Batch or code number, aDd
Net mass of the contents.

5.2.1 The words liquid glucole' ( ,,, 5.2 <a)] .hall be marked in
sufficiently bold letters as compared with other paniculan:

&""'1":
LlQ,UID GLUCOa
Intermediate Convenion
XYZ &. Co
20 Mar 1974
Batch No. 593

Net mass-25 kg
5.2.2 The product may also be marked with Standard mark.
5.2.', The use of tbe Standard Mark is governed by the provisions of the
Bureau a/Indian Standards Act, 1986 and the Rules and Regulations made
thereunder, The details of conditions under whicb tbe licence Cor tbe use of
Standard Mark may be gnntcd to manufacturers or producers may be obtained
(rom tbe Bureau of Indian Standards.

I. SAMPLING
6.1 The method of drawing representative samples of the material and
the criteria for conformity shall be as given in Appeadix G.

7. TBIT8
7.1 The testa .hall be carried out as prescribed in 4.2, U and col 4
aDd 5 or Table J.

74 Q,tIaue, of ~ - Unless specified otherwiae, pure chemicaJa

aDd cliatiDccI water (_IS: 1070-1960 ) Ihall be employed in tat..

ftIUItI.,...,.

Non _.... ....Iea.. ...1 mean chemica" that do

. . . . . . . 1M

SpeciftcatioDW. ~tft'........ qaaIity ( ,,.,,.).

DOt

coatalD lmpurI"

11,171. __

APPBNDIX A
( Claar, 4.2 )
DB~A'I10N

or

COLOn

A..e. GBNBUL

or

&.1.1 This method determin. the colour a 50 percent ( -/.) IOlatioa

of the material in water, by comparison with Lovibond 1Iaae. oIbowa
colour characteriaticl_

.&-1. APPAllATUI
A-l.11.nIIaa..t 11ateaaeter
A-l.2 GIaM Cell - 2-54-cm, made of clear, coIouriea glau.

U DOCBDURB
Ul Pnpuad.. of . . 8oIad. . - Prepare a 50

percIIlt ( -/, )

dation or the R.egular Convenion grade material in water.

A-2.2 Clean the 2M-cm glas. cell with carbon tetrachloride to remove
any oily or arearY film on it, and allow it to dry. Fill the cell with the
~pared 101ution ('11 A-2.1) and place it in position in the Tmtometcr.
Place alODl aide or it IUch red aiidJor yellow Lovibond ,~ u are
D_ry to match the colour shade of the prepared 101utioo. obIerviIw
the coloun the prepared lOlution and
the combiDation
LoviboDa
glula through the eye-piece.

or

or

or

A-& IlBPOIlT
W.I Report the colour or the preplred IOlution (COD~~t
-/_ of the material) in terms of Lovibond UDiti by
up
iDdiviclually the value. for the red and yellow LoviboDd gta-
:
Colour ~ of a 50 percent
(-/_) lO1utioD of the material
ill water. iD 2M-em cell OIl

.....

the LovibaDcllCa1e

.Y + 6R

where
- the . . . total or the various yellow (Y) Loviboad ___
\

6 - the . . total 01 the veiOUI red (1l) LoviboDd

UMd.

at--

A.PPBNDIX B
( C14us, 4.3 )
_ATlON

or DBXTIlOIB BQ,UIVALENT VALUB

-'1. JllUNCIPLB
8-1.1 Titration of the sugar solution with Fehling'. solution at boiling
point UIing methylene blue .. indicator. The voluma of the solutions
are acijuated in Ncb a way that the total volume at the end orthe titratioD
hal Biven value ( 75 :I: 5 ml ).

-.2. UAGBN'n

'eWbaI'-

-.a.1
801. . .
Solution A - Aqueous IOlutiOD or copper sulphate containiDg 69-28 I
or CuSO" 58.0 per litre.
Solution B- Aqueoua IOlution containing 346 I of lOdium ~um
tartrate, potallium tetrahycltate aDd 100 g of lOdium
hydroxide per litre.
NoD -,.. the mixed lOIutioal are Ullltable Ja die praeace or air.1DiDDI II claDe

. . . . . . . . 1...

MI.I CtIlUw-.. t/'" PIIalia(1 SoltdiOfI- Titrate the Fehlinl'llolution

tbua ~ with the ItaDdarcl101utioD of dextrole ( 11-2.2 ) .. indicated
lD MI. Let V, be the DUlDber of milli1itrel of deztroae IOlution uaed;
tbiI value ahould be equal to 40':1: 05 mi. Otherwise, the FebIiDI'l
IOhatioD ahould be adjusted.
114.2 laleelercl 1oI11t1oa 01 Baa. . - Diaolve 2-5 I of the ewe
dauoee dried beforelwul at 700 UDder reduced preuure, in diat1I1ed
water to make 1000 mL
a.u II8dIJI-e III.. WI..- - ODe perceat aqueoUi lolutioD.
Nt AWAaATUI
N.1 0nliDary laboratory equipmet.
NeLl V__" " " " - 500 mL
MI.2 . " . M.t Pilfllll .... 10 mL

MI.I..."B'"
NeIA CAn....",

N.I ~

COlI'"

01 tbelallGWiDl parIL

111171 1174

II-U.I CMiI., FW .IA II N.,.,. Moali- 300 mi.

a&2.2 B,.",. IIIiIA SItIHIt ", S. B.",,, ... 50 mlgraduated in 01 mI

aDdlUitable protection plate.

N.2.3 .A H , DIIIiu -- which we>uld .ure bo~ under the

coaditioDI indicated in B.f and which would enable lightiDg. to determine
the ead point without baviDg to move the conical fluk.

or

MI T. . ~-eIea-

Weil~ to the nearest 0001 g a quantity

IUDple
a way that Uter dilution to 500 mi. the test lolution containl
approximately 025 I or .-educing 'ucan expressed al dextrose for 100 mi.
(This teat portion generally weighs between 1 and 10 g. )

in

IUch

a.t.2 Det.....-d. . - With the help oi the pipette, transfer 10 ml each

or IOluUoDI A and B or the Fehling'. solution into

the 300-ml conical

fIuk. Add. boiling replatOf, that ii, pumice atone or glaa marblCl
aDd enough of water to raise the total volume of the liquid (Fehling'.
IOlution + water + tat solution) to 75 ml :I: 5 ml at the end of the

0_

titration. In order to determine the quantity of water that is added, it is

neceaary to eany out a preliminary determination using the quantity
of water. considered efFective for covering evaporation, for inltaace 40 mi.
ht the conical ftuk on the heating device. Ilisht from the ltart of
'-tiDI. pour with the help of the burette the sugar lO1utioD amounting
witbia 0-5 m1 of the anticipated eDd point (determined by preliminary
tell in which the tat solution is pdually added tiD the end. point is
reached)_ Adjult the heating in IUch a way as to make the 101ution boil
within 2-75 0-25 min and then make DO more adjustment till the eDd
of the tCit. The boiliDl should be brisk and coDtiDuoUi all alODI the
~tioD otherwise air would enter the fIuk and oxidize itl cooteat..
It is therefore euential that the flask it DOt lhaken after the heatinS atartI.
After 2 minuta of boiliDI. add 2 drops of methylene blue lOIution aDd
complete the tittation drop by drop. At the approach of the end point,
obIeIYe time of teD to Been teeODcIa between the addition of two
IUCClllive _dro~. Carry oat the operation till the blue colour vaaiaheL
TItradoD. Ihou1cI be comPleted withia 1-' to 21 min after the aclditioD 01

the iDdicator.

Ml.1 Le& VI be the Damber or mDlDitres or tat IOlution UIed. If this

901- 11110I 40 5 ml. it II MeIII.ry to moctiry the CODCefttratioD of the

tilt ICIIudoa. It II reawnlllf:Dded to check at the ad of the titration if the

1Dal.a-1WII witbia the prelCribod limits. Carry out two dctermiDatiolll
- _ . . . .mpIe.

II a171 1174
11-5. BXnBIIION

or BBlULD

11-5.1 The reducing IUpr expreued u dextrose, percent by mau, is equal to:
2-5

iOOO x V, x

see
y;-

100

125 V,
X E

E - Yi

where

E - mau, in g, of the telt portion;

V. - volume, in ml, or the standard dextrose solution (8-2.2)

used for the calibration of the Fehling'. 101ution; and .

VI .. volume, in mit of the test solution required for reducins
20 ml of the Fehling'. IOlution.

APPENDIX C
[Tal, 1. 111m (i) ]
DlC1DIIINA110N 01 TOTAL SOLIDI
e-l. BQ,UlPMENT AND APPARATUI
c.l~

The foUowinl equipment and apparatUi are required.

04.1 Dlatomaceo_ BanIa - Neutral to Utmul when moilteDed .with

water. [ If. commercial grade iI used, wash it by percolation with water
which baa been slightly acidulated with hydrochloric acid, until the eftIuent
is acidic to lltmUi. Then rewuh with water (not acidulated), UDlll the
eftluent is DeUtral to litmul. Finally dry the earth in an oven at
about 10500. J

c.l.2... Idrft.l RocI - approximately 6O-mm 10Dl with a

flattened end.

C-l.3 Mol.a... Dl8Ia - with a cover made of aluminium; approximately

25 mm in h~ilht and 75 mm in diameter.
C.l.t Desiccator - containq pholphorua pentoxide .. desiccant.
Q.l.5 NicILelleeap
o.J.6 \'aca o..a

10

C4. PaOCBDlJU
e-2.1 Place about 10 I of the diatomaceoua earth aDd the llul ltirriDg
rod in the moisture dish aDd dry the -diatomaceoUi earth ill an air OVeD at
about 105C. Cool in the daiccator aDd wash. R.epeat dryiq in, the
air oven. cooling in the desiccator and weighing till the mas. I. CoDltaDt.
Note the combined mall of the cliIb with the cover, the II. . ItirriDI rod
and the dry diatomaceous earth. Wash accurately about .5 I
the
material in the nicke1lCOOp, miK it with .5 mI of water and run 011 to the
diatomaceoUI earth, uaing the glall stirring rod. Wash the scoop thrice,
using 2 m1 of water for each washing and tran.fer the washiDp on to
diatomaceous earth. limultaneously workiDI the contents of the moisture
dish into a thick paate uainc the llua ltirr!q rod. Leave the 1_
stirring rod in the diaL Place the moisture dish in the vacuum oven and
dry the contents or dish at 1000. Cool the dilh and its conteotl in the
desiccator and wash. llepeat dryinl in the vacuum oven cooliDs in the
daiccator and weiahiDI till the mall is CODitant.

or

e-s.

CAl..CULATiON
TotailOUcb coatent,
percent by IDUI

100 -

[ 100 ( AI

+ ~ - B1 ]

AI

where

in I of the material taken ror the tat;

~ - IIWI ill I of the moisture cliah containing the IIua stirring rod and dried diatomacooua earth; aDd
B - mall in I of the lDOilture dish COD~ the sJ"
It~ rod, diatomaceous earth and the dehydr4ltecl
material.

],I -

IDUI

APPENDIX D
[Tall 1, 1_ (v) )
DBIaIIINATION 01' AIlIBNIC

0.1. AWAIlA'I11I
1).1.0 The followiD, apparatUi aacmbled u ihown in Fil. I is required.
0.1.1 WWelia dt-..-12OmL

11

II

117.-

D-l.2 . . . T_ - made from ordinary gIaa tubiDl, aDd bavinla total

leDp or 200 DUD. It bu an iatcmal diameter of cuetly 6-5 IDDl aDda.
aterDal diameter of about 8 DUD. It it drawn out at one eDd to a
diameter of about ODe mDlimetre and a hole DOt Iell thaD 2 mID in
diameter II blown iA the aide of the tube, Dear the COllltricted pan. DIe
upper ead of the tube is cut off.quare aDd iI either IO'&Ddcd off llichdy

FOund amooth.

_Ifl"- ... OIA

~J

...

.....

_.. .....
. .".

or I . . . "

.......
FaD. 1 ,A_eLY'ow

Aw~

DIrnaImrA'rDI eA ....
II

WIa

II a171 1174
One fitl exactly into the mouth of the
wide mouth bottle and has a hole bored centraUy to take the tube from
its conatricted end. Each of the other two rubber bunp (about
25 X 25 mm) hal a hole, exactly 6-5 mm in diameter, bored centraDy
and is fitted with a rubber band or spring clip for holding them tichtly
together_

D-l.J IlaWIer 11_. - three.

0'

1).1.4 P........tloa
the Ola Tabe - Moisten a Imall quantity of
cotton wool with lead acetate solution (s" 0-2.1) and then dry it in
dUlt-free atmosphere, Lightly pack the glau tube with this cotton wool.
10 that the upper surface of the cotton wool is not leiS than 25 nun below
the top of the tube. Insert the upper end of the tube into the nurow
end, of one of the pair of rubber bungs (about 25 X 25 mm lize) either
to a depth of about 10 mm ( when the tube hal a rounded oft' end) or 10
that the ground end of the tube is flush with the larger end of the bUIll.
Place a piece of mercuric chloride paper (s" D-2.2 ) flat on the top of the
bung. Place the other bung over this with itl larger end in contact with
the piece of mercun-e chloride paper. Falten the two bungs by means
of the rubber band or the spring clip, in IUcb a manner that the
of the two bunp ( or the upper bung and the glalS tube) meet to form

bom,.

true tube of 65 mm diameter interrupted by diaphragm of mercuric

chloride paper.
1).1.4.1 Initead of this method of attaching the mercuric chloride paj)Cr,
any other method may be used, provided. that: (a) the whole orthe
evolved p i pusa through the paper; (b) the portion of the paper in
contact with the p i is a circle of 65 mm diameter. and (c) the paper iI
protected from IUDliSht duril1l the test,

0.2. ItBAGBNft
D-U The Collowinl reagentl arc required. (The reagenta with the
aception of 0.2.10 aDd D-2.11 abal1 be free from traeea of aneaic. )

D-2.1 LeU Acetate Wad.D -10-0 percent (III''') ill water, reeendy
boiled and cooled.

D-U Mwcnarle aaa-Ici. Papa- - smooth white filter paper.

Dot leu
than 25 mm in width, lOakecl in a laturated lolution of mercuric chloride

in water; ~ed to remove lu~rftuoUi

lolution. and dried at about &ere

ill the dark. The pUe of the filter paper .haD be iucb that the lUll ill
!:i~ Ibal1 be betWeea 65 aDd 120; the thickncu in mID of 400 papen
be .p~tely equal, numerically, to the IDUI in IIml Mercuric
chloride 'p.per ~ be ltored in a .toppered botde in die clark. Pa~
which have been Gpoled to aunUght or to the vapour or ammODia abOuId
DOt be UIecl u they pve ~ter Colourccl .taiDI or DO .taIa at aII wbeD
employed iDthe quuatitadYe tat ror aneaic.

IS

111171U7t

or

D-U aw.......I.... - Acld to a amaU quantity of water !O I

potaaium bromide. DiIIoIve the potaIIium bromide in the water ADd
mix with it 30 I of bromine. Doute with water to produce JOO mla.
mi&.

D-U eoa.....tecl Byclroaa.Ic AcltI- sp If 1-16 and complyiDg

with the (oUowinc tat:
Dilute 10 m1 of the concentrated hydrochloric acid with sufficient

water to produce 50 ml, add 5 m1 of ammonium thiocy~te aoIution

[ 10 percent ( JIll' ) in water] and .tir immediately_ No colour shall
be produced.
1).2.5 . . . . . .teII HfClreclaloric AelcI- Mix together one millilitre of
6romine solution aDd 100 m1 of concentrated hydrochloric acid.

or

D-2.1 ltaDaoa CIaIorIde SoIad. - Dilute 60 mI

concentrated
hydrochloric acid with 20 m1 of water, add to it 20 I of tin, heat geDtly
uDtiJ gas ceuel to evolve, and add sufficient water to produce JOO ml,
aUowiDS the undiuolved tin to remain in the solution. Decant the clear
.oIution. add aD equal volume of concentrated hydrochloric acid, boil
clown to the origiDal volume and filter through a fine-grained filter paper.
B-2.7 P. . . . . . . locIId. - crystals or powder.

D-U Zlac - granulated and complying with the following test:

Take 50 m1 of water. 10 mJ of stannated hydrochloric acid
( ,. D4.I ) and 0-1 ml of dilute solution of arlCDic (I" D-2.11 ) in
the wide mouth bottle. Add one gram of potassium iodide and 10 I
of zinc. ~ place the prepared glau tube (I. 1).1.4)' in
position. Allow the reaction to continue for one hour. A faiDi
but diatiDet yellow .tain thall be producod on the merewic
chloride paPfL--

D-U Iea...tell B,uocJa1orle Aeld - Mix together one millilitre of

chloride lO1utiOll and 100 mJ of concentrated hyclroch1oric ae~.

1taDD0UI

D4.10 IU-I 80JadMa ., Anealc - DiIIoIve 0-132 I of arsenic

trioxide in 50 ml or CODCeDtrated hydrochloric acid, 8dcl lufBcient water
to produce 100 ml and mix.
B-UI DIhd. W1dI_ e6 AneJIIc - frahly prepared. Dilute ODe
mUliUtre olltrODl lO1utioD of anenic with water aUlident to produce
JOO mi. ThiI dutioD coataiDI (1)J JDI 01 aneaUc (or 0-013 2
or
MAl perml.

DI,

D-S. BCKDtJD
D4al~ e t . W."-W~~ 10loltbe

mat.w, cO.oIve it Ia 50 mI or water,. . acid to It

14

10

iII-oIl1ittt1,IM

ted

IItl7l.11H
hyclrochloric aciel. AUow the IOhatioD to ItaDd for five minutea aacl
remove the excea of bromine with few drops or stannous chloride
lO1ution.
D-I.2 Place the whole of the prepared IOlutioD (1M 1).3.1) in the wide
mouth botde and add 04e gram of potulium iodide and 109 of aiDe.
Qpickly place the prepared gJaIl tube ( III D-I.t) in ~itiOl1. Allow the
reaction to continue (or 40 minut. ( III.u. Note under D-3.2.3). Remove
the piece of mercuric chloride paper at the end of this period. Compare the
yellow stain, produced on the mercuric chloride paper if arsenic i.
praent in the material, by day light with the standard ,tain prepared u
ilacnDed under D-U.

o.UeI The limit prescribed in Table 1 shall be taken as not havial

been exceeded if the Itain produced by operating the material is Dot
deeper thaD the standard Itain ( ,,, B-3.3 ).
D-S.2.2 ComJNltisOfi .f SItJias - The compariaon of the stains is made
with freshly prepared atain immediately at the completion of the tat.
D-S.2.S A blank determination shall be carried out under the same
eonditions, on the same reagents [used both in the preparation of the
solution ( '" 1).3.1 ) and in the test (
D-S.2)] and by the same perIOD
but without using the material. The blank ahalI Dot produce any
visible ltain on the mercuric chloride paper.

s.

HOI'JI - The reactioD may be accelerated by p~cj.1 the apparatul QD wanD

aurI.ce. care beiDi takeD that the mercuric chloride paper mDaIDa quite dry
tiLroupout the test. The IDOIt luitable temperature lor carryiDa out the teet II
paeraJly_ about
but becauae the rate evolution the PI YUiellOIDeWbat
with different batches or sine, the temperature may be adjuated to obtalD. red1itb
but DOt too violeat. evolution 01....
tube .houJcl be wubcd wite
coaceDtrated h,drochlorlc acid, riueCl with water, aDd dried betweea ~.
teItI.

we.

or

The'"

or

B-I.s Jtnpan....., ltaallaftlltala - Mix together 50 mI of water,

or

10 ml of stannated hydrochloric acid and one miUilitre

dilute IOlu"
of aneDic. Treat the resulting solution as described UDder D&2 to
prepare the standard stain.

A.PPBNDIX B
[ TuZ, 1, 1"", (vi) ]
D_A110N 01' COPRa

11-1. UAGDrn
Il-I.l '-m

B,-...w. - CODCeDtrated.
15

11,171- 1174
8-1.2

c.lMa TetnelalwWe - recliatiUecl before use,

II."'D.

or

B-I.s
801. . . . - Diuolve 200 I
reagent grade ammonium
citrate and 50 I of reagent grade dUodium salt
ethylenediamine tetraacetic acid (Vonene) in distilled water in a one-litre volumetric fIuk.
DUute to volume with diltilled water and mix thoroughly.

8-1.t

lea.,

or

Coppe loIatl...

or

8-1.f.l Slid So,,,'iMI- Tranafer quptitative1y 02015 B

rea-,ent
grade anhydrous copper sulphate ( CuSC!>a) to a one-litre volumetric ftuk.
Dilute to volume with distilled water and mix thoroughly.
1l-1.4.2 $11III4.4 Di"", SoW. - 2 IC/ml. Pipette 250 m1 or stock
IOlution into a one-litre volumetric flask; dilute to volume with dUtllled
water aDd mix thoroughly. Prepare frahly.

B-l.5 CnMI RecI.lacll.__ - 0-1 percent IOlution.

Il-IA Car___ ReaJeat - 0-1 percent IOlution. DillOlve 02 I of
lOClium diethyl-dithiocarbamate in distilled ~ater. dilute to 200 m1 volume
and mix thoroughly. Filter, if the lolution il bazy. Prepare fresh daily.

8-2. PROCBDUIlB

1I-2e1 WeiSh accurately 2 of the aample and tr.Dlrer to 250-ml

~ratinl Funnel. Add mltaUed water to make a total volume 0(25 ml;
add 10 m1 of mukin. 101ution Uld 2 cIropi of indicator and mise
Neutralize to purple colour with concentrated ammonium hydroXIde and
add 2 drops in esceu. Add 10 ml ofcarbamate reagent and mix. Extract
with 5-ml portioDi of carbon tetrachloride until the final extract i.
colourlaa (four extractions are uauaUy adequate). Dry the combined
_tracts with reaaent grade anhydroUi lodium lulphate. Filter the dry
estract an4 wasb the filter pper with carbon tet...cbloride. Make the
volume of the filtrate to 25 ml with carbon tetrachloride and measure the
abIorptiOD at' 433 DID ia to 111m cell. Simultaneously, carry out blank
determinations on water aDd the reagentl. Extract With S-ml portions of
carbontetrachloride, and dBute
combined extracts to 25 ml with COl,;
the colour of the lO1utipniI Dot deeper than that of jhe carbontetrachloride
atractl deri)'ed from s-mf of ltaDdarcllOlutiOD, when aimilarly treated.

8-2-2 Prepare a lelia of ltaDdardi by treating aliquot. of the ltanrlarcl

dilute IOluti~D (B-l.~) of copper in the same manner al the teat
IOlutioft. Prom the ablOrption of the Itandard solutioDi. pre~e a
ItaDClard curve plottiDl ablo~tioD values apinst conCeDtratiOD. 'rom
tbiI curve, obtaill the mall of copper.
16

111171 1t7t

APPENDIX r
[ Tablll, 111m (vii) ]
DBTBRMINA.TlON OF J.LU)
~-1. IlBAGBNTI -

All reagents should be free (rom lead.

'-1.1 BJelroclalonc A.cld- 32 percent III/W.

'-1.2 . . . . . .' ..01 BI Sol.dea - Warm 0'1 B of bromoth~l

with 3'2 ml of N/20 lOdium .hydroxide and 5 m1 or alCohol

~lue

\ 90 pereent ): after solution is effected. and sufficient alcohol (20 percaat)

250 mi.
.

to produce

P-IeS AmID'" 801ad.. StI'ODl - contains not leu than 27 percent

-,. and not more than SO percent fII'm of NH.

P-l.4 25 Pereeat Cltftc Add Solatloa AclJuted to 11-1,B - Dwolve

25 g citric acid in minimum quantity of water, cool and slowly add ItroDl
ammonia solution to acljust 1\1 pH and make total volume to 100 mi.

P-l.5 Pota1IIIl Cyaalde 8olatlo. - Dissolve 10 g of potaaium

cyanide in 90 ml of water, add 2 mJ oC hydrogen peroxide lolution
( 6 percent _/" of H,O. ). Allow to stand for twenty four hours. and make
up to 100 ml with water. Mix 2 ml with 5 ml of ammonia IOlution Ptt
and 40 ml of water and add 5 ml of dilute lead solution PI; no darkening
is produced.

P-l.6 D1phea)'lthlocarba. . . Ia Chloroform - 0'002 perceat ./,1.

P-l.7 Nitric Add 8olvdoD - 1 percent II/V, prepared by diluting I ml

of concentrated nitric acid (69 to 71 percent ml", HNO.) to 100 ml with
water.
F-l.l AllUDoaI... Cltnte SoI.doD - Dissolve with cooling, 500 I of
citric acid in a mixture of 200 ml of water and 200 mI of stroDi
ammonia soluti- .n. Filter and add sufficient water to produce 1000 mi.
1'-1.9 L_d 801.tlO8, Str_l- Dissolve 0'16 g lead nitrate in S ml of .
concentrated nitric acid (69 to 71 percent ",'v H~O. ) and add auflicient
water to produce 100 mi.
1'-1.10 Lead 801ad... Dnate - Dilute 1 ml
lufficient water to produce 100 mi.

1'-2. PROCEDURE

or strong lead solution with

ira..,.

~.2.1 Dislolve 10 I of the mat~rial in 20 ml of water,

to
leparatinl runnel wi~h the aid 01 ~ m1 o~w.tert acid 2'5 mI orhydrocbloric

17

111171- 1174
acid and lhake for five minut... Aclcl 5 drops of bromothymol blue
lO1ution and lufficient Itrong ammonia solution to produce a full blue
colour and add 15 ml in exceu. Add I ml of a solution prepared by
previously adjusting a 25 percent _/, solution of citric acid to pH 11 wit h
.tro~ ammoQia solution. 1 ml of potauium cyanide IOlutioD and
lufticlcnt of a 0002 percent miD solution of diphenylthiocarbazone in
chloroform until on shaking. the chloroform layer becomes purple or blue.
Separate the chloroform layer and add to the aqueous layer 2 ml of the
diphenylthiocarbazone solution. shake and separate the chloroform layer.
To the combined chloroform solutionI add 10 ml of a 1 percent (,I')
solution of nitric acid, shake until the chloroform layer becoma green,
allow to separate and discard the chloroform layer. To the aqueous
layer add 02 ml of t.he ammonium citrate sclution, 0-25 ml of Itrong
ammonia solution and 02 ml of potassium cyanide solution and sufficient
of the diphenylthiocarbazone solution until on shaking, the chloroform
layer becomes purple or blue. Transfer the contents of the .eparating
funnel to a stoppered tube. Repeat the operations omitting the substal)ce
under examination and using the same quantities of the reagents, 'To the
tube containing the blank, add dilute lead solution until on shaking, the
colour of the chloroform layer matches that of .the chloroform layer
obtained from the substance under examination. Not more than 2-0 ml
of dilute lead solution i. required. The limit prescribed in the material
specification shall be taken as not having been exceeded if the jlltenlity
of itl colour produced in the test with the material is not more than the
one produced with the corresponding lead solution.

APPENDIX G
( CIGUS, 6.1 )
SAMPLING OW UQ,tJID GLUCOSB

0.1. OBNBRAL UQ,UlRBMBNTS rOR SAMPLtNG

0.1.0 In drawilll. preparing. storing and handling aamples, the following
precautionI and dlrections shall be observed.

0-1.1 Sampliq iDItrUment ahall be dean and dry when uaed.

0-1.2 Precautions mall be taken to draw the lample. in a manner

10 U

to protect the .amples, the material being sampled, the Ial1)plinl

WtrumODt and the containerl for samples from adventitious
contamination.
G-Iel The aampla ahall be placed in clean and dry glalS eontainen.
J8

1I.1IS-1I7.
Q.IA The ample containen ,hall be I~led air-tisht after fiI1iDI and
marked with full details of sampling, date of manufacture. Dame or
manufacturer and other important particulars of the coDlignment.

0-1.5 Samples shall be stored in such a manner that the conditioDi of

storage do not unduly affect the quality of the material.

G-2. SCALE OF SAMPLING

0.2.1 Lot - All the containers in a lingle consignment of the material
of the same grade, drawn from a lingle batch of manufacture ahall
coDititute a lot. If a consignment is declared to consist of different batches
of manufacture, the containers belonging to the same grade and batch
shall be grouped together. Each group shall constitute separate loti.
G4.2 On Sample - For the purpose of drawing samples for test, one
container from each lot shall be selected by the purchaser. This contaiDer
shall constitute the grOIl sample.
0-2.2.1 The container selected for the gross sample shall not be damaged
or leaky and shall have its seal intact.

0-,. PROCEDURE

FOR DRAWING A SAMPLE FROM TIlE

CONTAINER

G-I.l S.m...... Pa - For the purpose of drawing a sample of liquid

Jrlucose from the container, it is recommended that a pump of the type
shown in Fig. 2 may be used. The pump is made of copper. The tube
of the pump is 2 mm thick. with an internal diameter of 42 mm. The
tube i. provided with a downward bent spout at 76 mm from the upper
end of the screw top. The spout i. of 19 mm internal diameter. The
piston is provided with lifting up valves. The piston rod is 8 mm in
diameter and i, made
copper. The rod is provided witb a locking
device at itl top 10 that it may not have to be kept preaed down while the
material rises up inside the tube.

or

Q.J.2 Method . , lampUaI- Place the container selected for the

purpose or .ampling at a clean and dry place. Thoroughly clean the lid
of the opening or the container and the area round about the opening
with IO&p and water. Wipe the cleaned area dry. Remove the lid
the opening and take out with a ladle sufficient quantity of the material
to remove the top scum. if any. Insert the pump (s" G-S.l) with itl
pilton locked in positiOD. throulfh the openinR of the container and very
Ilowly presl the pump down to the extent of the upper one-third height of
the container. Hold the pump in thia position for five minutes. ARAin very
slowly preal the pump down through the middle one-third he~ht ofthe container and hold the pump in tbat poaition for five minutea. Prell the pump
down again VPry Ilowly and when it touches the bottOID of the container.

or

It

18 I 873 1974

LOC'"
DIYC.

ENLARGED SECTION AT X X
LOCKiNG DEVICE

g
............-.DI.

All dilllCDlbaa AD aaiIIimelftl.

Flo. 2 SAalPuNo Pmo

20

111171 lt7t
raile it a little 10 that it is just above the bottom of the container. Allow
the pump to remain in that position again for five miDuta. (The pump
will have been filled in by thia time. ) Take out the pump aDd .wipe ita
outside surface 10 as to remove the material which may be adheriDg to it.
Unlock the piston and work it very slowly through one complete upward
movement, simuJtan:ously, collecting the material flowing out of the
spout in a perfectly clean and dry sample container. Repeat the operation
of drawing samples in the above manner, until a quantity of about 2 kg of
the material is collected in the sample container.

G-4. TEST SAMPLES AND RBI'ERBE SAMPLE

0'

G-f.l .........do.
Te8t ....pl.. - To prepare test samples,
thoroughly mix the material drawn as specified under G-S.2 and divide it
into three equal parts, each part ( test sample) being not lesa than 0-6 kg.
Transfer these teat samples to thoroughly clean and dry sample containers,
and seal them air-tight. Label the sample containen with all the
particulars given under 0-1.4. One of these samples shall be lent to the
purchaser and one to the vendor.

G-t.2 Rei.... . ...pl. - The third sample, bearing the seals of the
purchaser and the vender, shall constitute the referee aample to be uaed
m case of dilpute between the purchaser and the vendor. It shan be kept
at a place agreed between the purchaser and the vendor.

G-5. _

O:r TaTS AND CRITBIUA :rOil COIUOallllY

0-5.1 The test sample as prepared under G-U shaD be tested tor all
characteriatiCl ('11.) of this lpecification.

G-5.2 The lot .hall be cleclared as conforming to this lpecificatioD if the

test raulta aatilfy the requirements or each of the characteriltia.

21

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Hdq. ." ....

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KOLKATA700072

2371086

ts-.. ~ .. at Nowtty a-ma.r., Grent Ao.d, MUMSAI400007

3011521

PrInted at NM IndIIl PrInting Pr , KhU.... , Incla