Beruflich Dokumente
Kultur Dokumente
Bishwajit Mazumder
Nursing Instructor
Dhaka Nursing College,
Dhaka
Subject: General Biology
GENETICS
1. Mendelian genetics
2. DNA and RNA
3. Gene Expression and regulation
4. Genetic Engineering (including providing knowledge on IVF)
The Mendelian Concept of a Gene
Gregor Mendel
In the 1860s, an Austrian monk named Gregor Mendel introduced
a new theory of inheritance based on his experimental work with pea
plants. Prior to Mendel, most people believed inheritance was due to a
blending of parental essences, much like how mixing blue and yellow
paint will produce a green color. Mendel instead believed that heredity
is the result of discrete units of inheritance, and every single unit
(or gene) was independent in its actions in an individuals genome.
According to this Mendelian concept, inheritance of a trait depends on
the passing-on of these units. For any given trait, an individual inherits
one gene from each parent so that the individual has a pairing of two
genes. We now understand the alternate forms of these units as alleles.
If the two alleles that form the pair for a trait are identical, then the
individual is said to be homozygous and if the two genes are different,
then the individual is heterozygous for the trait.
Based on his pea plant studies, Mendel proposed that traits are
always controlled by single genes. However, modern studies have
revealed that most traits in humans are controlled by multiple genes as
well as environmental influences and do not necessarily exhibit a simple
Mendelian pattern of inheritance.
Mendels Experimental Results
Mendel carried out breeding experiments in his monasterys garden
to test inheritance patterns. He selectively cross-bred common pea plants
(Pisum sativum) with selected traits over several generations.
After crossing two plants which differed in a single trait (tall stems
vs. short stems, round peas vs. wrinkled peas, purple flowers vs. white
flowers, etc), Mendel discovered that the next generation, the F1 (first
filial generation), was comprised entirely of individuals exhibiting only
one of the traits. However, when this generation was interbred, its
offspring, the F2 (second filial generation), showed a 3:1 ratio- three
individuals had the same trait as one parent and one individual had the
other parents trait.
Mendel then theorized that genes can be made up of three possible
pairings of heredity units, which he called factors: AA, Aa, and aa.
The big A represents the dominant factor and the little a represents the
recessive factor. In Mendels crosses, the starting plants were
homozygous AA or aa, the F1 generation were Aa, and the F2 generation
were AA, Aa, or aa. The interaction between these two determines the
physical trait that is visible to us.
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DNA
It usually occurs inside
nucleus and some cell
organelles. (Mitochondria
and chloroplast in plants)
It is double stranded with
exception of some viruses.
RNA
Very little RNA occurs inside the
nucleus. Most of it found inside
the cytoplasm.
Sl
no
between complementary
nitrogen bases of the opposite
stands (A-T, C-G).
DNA
RNA
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and countries of the Third World. Even though these two areas of
science may have seemed unrelated in their beginnings, the two have
now converged. In vitro fertilization (IVF), the fertilization of egg with
sperm in an external environment, provides embryos that may be
reimplanted into a woman. Most women who go on IVF programs will
not give birth to a live baby. These embryos are also the raw material
which allows genetic experimentation to be possible. They are in an
external laboratory environment, which means they are accessible for
manipulation.
The simultaneous development of DNA technology and IVF techniques
has brought science and society to a unique point the possibility of
gene manipulation, to correct genetic disorders. Such manipulation, if
carried out on embryos, will affect future generations irreversibly, and its
application to humans inherently reinforces a discrimination against
those who are differently able in our society. It seeks to eradicate
defective genes from future human populations. Also, the increased
emphasis on isolating the causes of diseases as genetic ones and
neglecting environmental factors can be likened to the theories of
sociobiology and biological determinism.