Beruflich Dokumente
Kultur Dokumente
A R T I C L E I N F O
A B S T R A C T
Article history:
Available online 22 October 2015
We used six types of urine test strips and cotton swab for comparing the DNA extraction efciency. DNA
extraction was performed with EZ1 Advanced XL, DNA typing with AmpFlSTR Identiler Plus PCR
amplication Kit, and electrophoresis with the Applied Biosystems 3130xl Genetic Analyzer. ComburTest, Uriet S, and UroPaper appear suitable for DNA extraction when using urine test strips as
presumptive testing reagents for potential blood.
2015 Published by Elsevier Ireland Ltd.
1. Introduction
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Table 1
List of urine test strips intended for clinical use.
Urine test strip
Distributer
Combur-Test 10UX
0.37.0
60 [3]
0.150.62
60 [4]
0.67.5
20 [5]
0.310.0
45 [6]
0.610.0
60 [7]
0.37.5
30 [8]
Hemastix
Uriase-Kc
U-TestVisual 5
UrietS-7UA
UroPaperIII Eiken
Cotton swab
mg Hemoglobin/ml urine.
Table 2
Results of study of DNA extraction efciency.
Urine test strip
Combur-Test 10UX
Hemastix
Uriase-Kc
U-TestVisual 5
UrietS-7UA
UroPaperIII Eiken
Cotton swab
Blood
4. Discussion
Immediately
0.0243
0.0022
0.0006
0.0008
0.0606
0.0266
0.0266
0.0325
0.0053
0.0008
0.002
0.0378
0.019
0.0319
0.1718
Table 3
Results of study of effects of reagent/blood complex.
Urine test strip
Color of mixture
Combur-Test 10UX
Hemastix
Uriase-Kc
U-TestVisual 5
UrietS-7UA
UroPaperIII Eiken
Cotton swab
Blood
Light yellow
Dark yellow
Very light yellow
Light yellow
Yellow
Very light yellow
0.4549
0.0208
0.0775
0.0338
0.403
0.3067
0.4792
0.5037
[5]
[6]
[7]
[8]
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