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Bahir Dar University College of Agriculture and Environmental Sciences Animal Production and Technology Program Course Title
 
 

Bahir Dar University College of Agriculture and Environmental Sciences

Animal Production and Technology Program

Course Title

Biotechnology in Animal Breeding

 

Course Code

Angb636

M.Sc. Program

Animal Genetics and Breeding

 

Mode of delivery

parallel

Module No.

02

Instructor

 

Office location: 30

 

Mobile:0931855328 ; e-mail: garikikumar@yahoo.com

 

ECTS Credits

5

(CP)

Contact Hours

Lecture

Tutorial

Lab/Practical

Home Study

Total

(per week)

2

-

-

8

5

Lecture days, Hours & Room:

Friday 8.30-10.15am

 

Tutorial/Lab days & Hours

NA

Target Group:

M.Sc. in Animal Genetics and Breeding

 

Year /Semester

Year 1 & semester 2

 

Pre-requisites

Status of the course

Compulsory

COURSE DESCRIPTION

Introduction: The basic tools for genome analysis. Molecular technology. Methods of gene isolation. Genetic engineering: insertion of foreign DNA and, gene transfer. Evaluation and dissemination of transgenic in animal production. Biochemical and DNA polymorphism. QTLs (Quantitative Trait Loci), Marker genes and gene mapping. Marker assisted selection in

animal breeding. PCR; Embryo manipulation. Embryonic stem cells and its application in animal breeding.

Course objectives: At the end of this course, the student will be able to:

  • In depth understanding of Biotechnology and genomics.

  • Increased awareness of the importance of genome analysis in research and applications. Expected/learning outcomes: students should able to know the different biotechnology procedures in the Animal genetics and breeding

week

Lecture

Conceptual Focus

Activities/tasks

(hrs)

1

2

Introduction –basic tools for genome analysis

-Listen to a lecture and take notes on the lesson treated,

2

2

Methods of gene isolation. Eukaryotic organelles and accessing the genome

-Take part in reading assignment, -Assignment

3

3

Methods of inserting of foreign DNA, Gene transfer technology. Transformation , transfection

submission -Forward all the confusion or doubts students may have in

relation to the

and somatic cell cloning

4,5

3

Primary and secondary cell culture Continuous cell lines. Application of cell culture technology in animal viral vaccines and pharmaceutical proteins

given lecture -Literature review and presentati on (seminar)

6

 

Structure of sperm and ovum

-Listen to a lecture

2

Cryopreservation of sperm and ova Artificial insemination Superovulation

and take notes on the lesson treated, -Take part in reading assignment,

-Assignment

7

2

-Synchronization of estrous

submission

Advantages and disadvantages

-Forward all the

   

Cryopreservation of embryos Ethics of embryo manipulation

confusion or doubts students

may have in

8,9

3

Transgenic manipulation of animal embryos Different applications of transgenic animal technology Animal Viral vectors

-Listen to a lecture and take notes on the lesson treated, -Take part in reading assignment, -Forward all the confusion or doubts students may have in relation to the given lecture -Literature review and presentati on (seminar)

10

3

Animal cloning –basics Cloning from embryonic stem cells and adult cells Cloning different animals Cloning for conservation and conservation of endangered species

Listen to a lecture and take notes on the lesson treated, -Take part in reading assignment, -Assignment submission

Social

and

ethical

issues

of

-Forward all the

cloning

confusion or

11

3

Biochemical

and

DNA

doubts students

polymorphism

 

may have in

Different

methods

in

relation to the

characterization

of

animal

given lecture

genomes

12

4

STR, QTL, RFLP, RAPD Genetic basis of disease resistance

13

1

Transgenic animal production and expression of therapeutic proteins

  • 14 2

 

Immunological and nucleic acid based detection methods for identification of animal species. PCR

 
  • 15 1

 

Marker

assisted

selection

in

animal breeding

 
  • 16 2

 

Embryonic

stem

cell and

 

applications in animal breeding

Course teaching/learning activities

The mode of the delivery of the course combines lectures, discussion, questioning and answering, readings, assignments, individual and /or group works and presentation.

Assessment Methods

Evaluation will be carried out based on continuous assessment which comprises:

midterm

Assignment

seminar

Final exam

Grading: As per the university’s regulation

COURSE POLICY

All students are expected to abide by the code of conduct of students (article 166 and 166.1.1, of The Senate Legislation of Bahir Dar University May 20, 2005) throughout this course. Academic dishonesty, including cheating, fabrication, and plagiarism will not be tolerated and will be reported to concerned bodies for action. Class activities will vary day to day, ranging from lectures to discussions. Students will be active participants in the course. You need to ask questions and raise issues. You are expected to do all the assignments submit and present you are supposed to accomplish. You are expected to attend class regularly. If you

miss more than 85% of the class attendance you will not sit for final exams. Please try to be on time for class. Cell phones MUST be turned of before entering the class as they are disruptive and annoying to all of us in the class. You are responsible for all class announcements and changes.

REFERENCES

Reproduction in farm animals-Hafeez and Hafeez 2008

Principles of genome analysis and genomics-SP primrose and RM twyman 2008

Discovering Genomics , proteomics and Bioinformatics- A Malcome Campbell and Laurie heyer 2010

Understanding Biotechnology- Aluizio Borem, Fabricio R santos and David Brown 2009

Genes VI Benjamin Lewin.

Approval section

Course instructor:

Dilip Garikipati

___________________________

Course chair:

Damitie Kabede

Department Head:

ViCe Dean

____________________________

Fentahun Meheret

____________________________

Hailu Mazengia

___________________________