Beruflich Dokumente
Kultur Dokumente
Syllabuses of the
Department of Microbiology
for
BS Honours Course
for the
Sessions: 2006-2007 to 2008-2009 and onward
Published by
THE UNIVERSITY OF DHAKA
BANGLADESH
2009
UNIVERSITY OF DHAKA
DEPARTMENT OF MICROBIOLOGY
Syllabus for 4 year BS (Honours) Course
Sessions: 2006-2007 to 2008-2009 and onward
To be eligible for admission into the 4-year BS, (Honours) courses in
Microbiology, the candidates must have Biology, Chemistry and Physics
including Mathematics in their HSC examination in Science group or
recognized equivalent examination. The educational qualification of the
candidates shall be decided as per University admission rules. The 4-year
(Honours) will span over 32 units of which year-wise course distribution has
been detailed below. Of these 32 units, 6 units shall have to be taken as extradepartmental courses. These 6 units will be, as of now, equally distributed in
the First Year and Second Year courses. Total marks of extra-departmental
courses will be added to the major Honours subjects for deciding grades.
During the final year course (Fourth Year), practical courses and/or research
projects will be assigned to the students, depending on their performance,
which wall be decided by the departmental academic committee.
The marks/units distribution in 4-year Honours course as follows:
First year
Departmental Course
3.5 units
Extra-Departmental course
3.0 units
Second Year
Departmental Course
4.5 units
Extra-Departmental Course
3.0 units
Third year
Departmental Course
8.5 units
Fourth year
Departmental Course
9.5 units
Total
32.0 units
One unit course consists of minimum of 60 lecture` hour duration and bears 100
marks. There should be one mid-term and one term-final examination for each
departmental course. 20% of the total marks shall be allocated for the mid-term
examination and allocated for the term-final. For each practical course, 40% marks
will be allocated for class records and regular participation in the laboratory works,
and 60% marks for the final examination. The extra-departmental courses shall
have no mid-term examination.
Course Name
Marks
Departmental Courses
Microbiol. 101
General Microbiology-I
50
Microbiol. 102
Microbial Ecology
50
Microbiol. 103
Microbial Chemistry
50
Microbiol. 104 Basic Techniques in
Microbiology
50
Microbiol. 105
Practical
1.0
100
Microbiol. 106
Viva-Voce
50
Extra-Departmental Courses
Microbiol. 107 Compute Applications
(Auxiliary)
Biochemistry II Basic Biochemistry
CM 100F Fundamentals of Chemistry
Total
Unit
0.5
0.5
0.5
0.5
0.5
1.0
100
1.0
1.0
6.5
100
100
650
Marks
Course No.
0.5
50
50
0.5
0.5
0.5
1.0
0.5
50
100
0.5
1.0
50
0.5
50
1.0
100
7.5
750
0.5
0.5
Course Name
Marks
Unit
Departmental Courses
50
50
50
100
50
Microbiol. 301
Virology-I
0.5
Microbial Metabolism-II
50
Microbiol. 303Microbial Molecular Genetics
Microbiol. 304
Medical Microbiology-II
50
Microbiol. 305
Immunology-I
100
Microbiol. 306
Agriculture Microbiology
50
Microbiol. 307
Food Microbiology-I
50
Microbiol. 308
Industrial Microbiology
50
Microbiol. 309
Enzymology
50
Microbiol. 310Pharmaceutical Microbiology
50
Microbiol. 311Microbiology of Frozen Food
and Fish
0.5
50
Microbiol. 302
Microbiol. 312
0.5
50
0.5
1.0
0.5
0.5
0.5
0.5
0.5
0.5
50
Practical
2.0
Viva-Voce
0.5
200
Microbiol. 313
50
Total
8.5
850
Course Name
Marks
Departmental Courses
Microbiol. 401
Virology-II
Microbiol. 402
Immunology-II
Microbiol. 403 Environmental Pollution and
Bioremediation
Microbiol 404 Food Microbiology-II
Microbiol. 405
Genetic Engineering
Microbiol. 406
Fermentation Technology
Microbiol. 407
Microbial Biotechnology
Microbiol. 408
Diagnostic Microbiology
Microbiol. 409
Analytical Microbiology
Microbiol. 410
Quality Control of Food,
Fish and Beverage
Microbiol. 411 Practical/Research Project
Microbiol. 412
Viva-Voce
1.0
0.5
1.0
0.5
0.5
2.0
100
50
0.5
0.5
1.0
0.5
1.0
50
200
0.5
100
50
2.
3.
50
50
100
50
100
4.
5.
6.
50
7.
Total
9.5
(0.5 Unit)
950
8.
9.
Books Recommended
1. General Microbiology G Schlegel HG & Kogut M
2. Biology of Microorganisms, 8th Edition Brock TD, Madigan MT,
Martinko JM & Parker J
3. Microbiology, 5th Edition Pelczer MJ Jr, Chan ECS & Krieg NR
4. Microbiology: An Introduction, 9th Edition Tortora GJ & Funke BR
5. Fundamental Principles of Bacteriology AJ Salle
(0.5 Unit)
(0.5 Unit)
(0.5 Unit)
(1.0 Unit)
(0.5 Unit)
(1.0 Unit)
1.
2.
3.
4.
5.
6.
Books Recommended
1. The Perspective. 1998 Hutchinson SE & Sawyer SC
2. SPSS for Windows: Base System Users Guide Release 6.0.
1995 Norusis MJ
3. Excel 7.0 for Windows in a Day, 1996 Stultz RA
4. Introduction to Computer Science PW Mursil PW &
Smith CL
5. Computer Net work Tanenbaun AS
6. An Introduction to Computer Hardware Cripps M
7. Computer Anatomy Rub N
1.
3.
4.
5.
6.
(0.5 Unit)
Books Recommended
1. Principles of Microbiology Atlas RM
2. Biology of Microorganisms, 8th Edition Brock TD, Madigan MT,
Martinko JM & Parker J
3. Microbiology Pelczer MJ Jr, Chan ECS & Krieg NR
4. Microbiology: An Introduction, 9th Edition Tortora GJ & Funke BR
2.
3.
4.
5.
6.
Books Recommended
Microbial Ecology: Fundamentals and Applications Atlas
RM & R Bartha R
Microbial Ecology: A Conceptual Approach Lynch JM & Poole
NJ
(0. 5 Unit)
2.
(0.5 Unit)
3.
4.
5.
6.
Books Recommended
1. Genetics Strickberger MW
2. Molecular Biology of the Gene, 6th Edition Watson JD et al.
3. Molecular Biology Freifelder D
4. Essential Genetics Russel PJ
5. Principles of Genetics Snustad DP, Simmons MJ & Jenkins JB
(0.5 Unit)
Books Recommended:
Jawetz, Melnick & Adelbergs Medical Microbiology, 24th
Edition Brooks GF, Carroll KC, Butel JS & Morse SA
Essential Clinical Microbiology: An Introductory Text
Cooke EM & Gibson GL
Manual of Clinical Microbiology, 4th Edition Lennette EH,
Ballows A, Hausler WJ Jr & Shadomy HJ
Modern Medical Microbiology Chowdhury MR
Medical Microbiology Duguld JP, Marinian BP & Swain RHA
(0.5 Unit)
Books Recommended
1. Introduction to Human Physiology Griffiths M
2. Human Physiology Schumddt RF & Thews G
3. Human Anatomy, Physiology and Pathophysiology
Thews G, Mustschler F & Vaupe P
(1.0 Unit)
(0.5 Unit)
(1.0 Unit)
2.
3.
4.
5.
6.
7.
8.
Microibiiol.
(0.5 Unit)
303:
Microbial
Molecular
Genetics
(0.5 Unit)
(1.0 Unit)
(0.5 Unit)
(0.5 Unit)
(0.5 Unit)
(0.5 Unit)
(2.0 Units)
Part A (Virology-I)
1. Cultivation and enumeration of bacteriophages
2. Isolation of bacteriophages from raw sewage
3. Detection of HBsAg from patients serum by serological
methods
4. Isolation of TMV virus and infecting plants
(0.5 Unit)
(1.0 Unit)
Books Recommended
1. Microbiology, 5th Edition Pelczer MJ Jr, Chan ECS &
Krieg NR
2. Biology of Microorganism, 8th Edition Brock TD, Madigan
MT, Martinko JM & Parker J
3. Fields Virology, Vol. I & II Knipe DM, Roizman B,
Howley PM, Straus SE & Griffin DE
4. Jawetz, Melnick & Adelbergs Medical Microbiology, 24th
Edition Brooks GF, Carroll KC, Butel JS & Morse SA
(0.5 Unit)
7.
(0.5 Unit)
3.
4.
5.
6.
7.
8.
(0.5 Unit)
Books Recommended
1. Principle of Gene Manipulation: An Introduction to Genetic
Engineering Old RW & Primrose SB
2. Molecular Biology of the Gene Watson JD et al.
3. Genetic Engineering Kingsman AJ & Kingsman SM
4. Principles of Genetics, 4th Edition Snustad DP & Simmon MJ
5. Gene Cloning: An Introduction Brown TA
6. Molecular Cloning A Laboratory Manual, 2nd Edition Sambrook J
& Maniatis T
7. Principle of Gene Manipulation: An Introduction Old RW
8. Current Protocol in Molecular Biology Smith JA & Struhl K
(0.5 Unit)
(1.0 Unit)
(0.5 Unit)
(1.0 Unit)
4.
5.
6.
7.
Books Recommended
1. Quality Control in the Food Industry, Vol. I Herschdoerfer
SM
(2.0 Units)
Part A (Virology-II)
Detection of viral Ags/Abs from patients sera by immunological
techniques
PCR amplification of HBV core and surface genes
Detection of viral DNA by PCR amplification and dot-blot
hybridisation
Use of RPHA method for the detection of viral Ag/Ab
Titration of virus using immunofluorescent microscope
Part B (Immunology-II)
1. Detection of antigen and antibody
a) by gel immunodiffusion technique
b) by radial immunodiffusion technique
c) by Crossed immunoelectrophoresis technique
2. SDS-PAGE and immunoblotting of bacterial proteins
3. Complement fixation tests
4. HLA typing
Part C (Environmental pollution)
1. Enrichment and isolation of biodegradative microbes from
environment
2. Non-culturable state of microorganisms (detection by FA or acridine
orange DVC)
3. Detection of indicators and pathogenic microbes in potable water
4. Water purification (viz., flocculation, chlorination, ozonation etc.)
Part D (Food Microbiology-II)
1. Detection of B. cereus and S. aureus in fast foods
2. Detection of E. coli and Aeromonas hvdrophila in salad dressings
3. Isolation of Aspergillus flavas from oil seeds
4. Detection of haemolysin and phospholipase C (toxins) from B. cereus
Part E (Genetic Engineering)
1. DNA digestion by restriction enzymes
2. Ligation of DNA to appropriate vector
3. Study of genetic map
Part F (Fermentations Technology)
1. Dough fermentation by baker's yeast for bread making
2. Production of acetic acid by Acetobacter aceti
3. Demonstration of a fermentor
4. Yogurt production by lactic starter
5. Production of citric acid by A. niger
(0.5 Unit)
(1.0 Unit)
animals
Practical (Marks: 20)
1. Handling and use of bright-field microscope
2. Staining techniques: simple staining, negative staining and
Gram staining
3. Preparation and examination of bacteriological culture
media
4. Transfer and maintenance of microbial pure culture
5. Techniques for isolation of pure cultures: pour plate, spread
plate and streak plate methods
6. Enumeration of microbial cells by using improved Neubauer
counting chamber
7. Enumeration of microbial cells by standard plate count
method
7. Demonstration of antimicrobial activities of antibiotics and
antimicrobial agents
(1 Unit)
characteristics
5. Lipids: nomenclature; classification; reactions of fatty acids; sterols
and methods of estimation; structure and biological functions of
different classes of lipids
6. Amino Acids and Peptides: structural features optical activity and
classification of amino acids ionization of solution; behaviour; colour
tests; isolation of amino acids from protein hydrolysates; peptide
bonds and biologically important peptides
7. Proteins: general introduction; classification based on shape, structure
and biological properties; isolation from natural sources; different
levels of structural organization (in brief); enzymes chemical nature;
Km value and Vmax; enzyme inhibition; digestive enzymes
8. Nucleosides and Nucleotides: basic chemistry of nucleosides and
nucleotides; polynucleotides
9. Vitamins: classification; occurrence; deficiency symptoms; biological
functions; vitamins as coenzymes
10. Laboratory Works:
a) Preparation of standard solution and standardization of HCl
b) Determination of calcium in biological sample
c) Determination of ascorbic acid component of biological sample
d) Colour test for biomolecules
e) Determination of lactose contents of milk
f) Determination of inorganic phosphorus content of the supplied
solution
3.
4.
5.
6.
7.
8.
2.
(1.0 Unit)
9.
Books Recommended
1. Chemistry of Organic Natural Products, Vol. I & II
Agarwal OP
2. Organic Chemistry Morriona RT & Boyd RN
3. Organic Chemistry, Vol. I & II Finer LL
4. Organic Chemistry Handrickson JB & Pine SH
5. Other Industrial Chemistry for Topics-7
(1.0 Unit)
Practical
1. To acquaint with the techniques for preparing temporary slides of
fungal specimens for microscopic examinations
2. Laboratory studies of the locally available members of the fungi
covered in theory
3. Techniques of growing fungi on culture media
Books Recommended
1. Introductory Myclogy, 4th Edition Alexopoulos CJ, Mims CW &
Blackwell M
2. Fungi Howker J & Lilian E
3. Fungi and Plant Diseases Mundkar BB
4. Fundamentals of the Fungi Moore-Landecker E
5. Introduction to Fungi Webster JG