SMK CHUNG HUA MIRI

BIOLOGY FORM 4
SEMESTER 1 EXAMINATION/ MAY 2016
1 HOUR 30 MINUTES
CLASS:

NAME:

MARKS :

/ 50

MARKS :

/ 50

MARKS :

/ 50

SMK CHUNG HUA MIRI

BIOLOGY FORM 4
SEMESTER 1 EXAMINATION/ MAY 2016
1 HOUR 30 MINUTES
CLASS:

NAME:

SMK CHUNG HUA MIRI

BIOLOGY FORM 4
SEMESTER 1 EXAMINATION/ MAY 2016
1 HOUR 30 MINUTES
CLASS:

NAME:

Answer all questions

Question 1 (33 marks)
An experiment was carried out to determine the concentration of sucrose solution which
is isotonic to the cell sap of potato strips.
Diagram 1.1 shows the set up of apparatus for this experiment. By using a cork borer, a
cylindrical potato strip with diameter 1cm was obtained. The potato strip was cut at 4 cm
long.

0.2M sucrose solution

Potato strip

Diagram 1.1
The mass for each of the potato strips is measured by using a compression balance.
Diagram 1.2 shows the initial mass for each of the potato strips.

111

0
g
90

30
60
Initial mass of potato strips: ______ g
Diagram 1.2

The experiment in diagram 1.1 was repeated by using different concentrations of sucrose
solution.
Table 1 below shows the results of this experiment.

Concentration of
sucrose solution

Final mass of potato strip after 1 hour (g)

(M)

0.2

0.4

222

0.6

0.8

Table 1

(a)

(i)

Record the initial mass of the potato strip in the space provided in Diagram
1.2 above.

(ii)

Record the final mass of the potato strip in the space provided in the Table 1
[3 marks]

(b)

(i)

State two different observations made from the table.

Observation 1:

Observation 2:

[3 marks]
(ii)

State the inference which corresponds to the observations in (b)(i).

333

Inference from observation 1:

Inference from observation 2:

[3 marks]
(c)

Complete Table 2 below based on this experiment.

Variable

Method to handle the variable

Manipulated variable:
____________________________
____________________________
____________________________
____________________________

_____________________________
_____________________________
_____________________________
_____________________________
_____________________________

Responding variable
____________________________
____________________________
____________________________
____________________________

_____________________________
_____________________________
_____________________________
_____________________________
_____________________________

Constant variable
____________________________

444

_____________________________

____________________________

_____________________________

____________________________

_____________________________

____________________________

_____________________________
_____________________________

Table 2

(d)

[3 marks]

State the hypothesis for this experiment.

[3 marks]
(e)

(i)

Construct a table and record all the data collected in this experiment.
Your table should have the following titles:
- Concentration of sucrose solution
- Initial mass of potato strips
- Final mass of potato strips
- Percentage of change in mass of potato strips

555

[3 marks]
(ii)

Use the graph paper to answer this question.

Using the data in (e)(i), draw a graph to show the relationship between the
percentage of change in mass of potato strips and the concentration of the
sucrose solution.
[3 marks]

(f)

Based on the graph in (e)(ii), state the concentration of the sucrose solution which is
isotonic to the concentration of the cell sap of the potatoes. Explain your answer.

[3 marks]
(g)

The potato strip from 0.6 M sucrose solution was taken out and was dried with
tissue paper. Then it was immersed in distilled water for 1 hour. Based on the result
of this experiment, predict the final mass of the potato strip. Explain your
prediction.

[3 marks]
(h)

Based on the results of this experiment, state the operational definition for osmosis.

[3 marks]
666

(i)

In another experiment, it was found that a mustard stem strip that was immersed in
0.8 % sodium chloride solution did not undergo a change in mass.
The following solutions are used in this experiment.

0.25 % sodium chloride solution, 0.65 % sodium chloride solution, 1.10 %

sodium chloride solution
Classify the above solutions into the table below.

Solution concentration (%)

Types of solution compared to the osmotic

concentration of the cell sap

[3 marks]
Question 2 (17 marks)
A hawker finds that washing the bowls in water containing 10ml of washing liquid which
contains enzymes is more effective than washing the bowls in water containing 5ml of the
same washing liquid.
Based on the situation, design a laboratory experiment to study the effect of enzyme
concentration on the rate of enzyme reaction.
The planning of your experiment must include the following aspects:
a) Problem statement

b) Hypothesis
c) Variables
d) List of apparatus and materials
e) Experimental procedure or method
f) Presentation of data
(17 marks)
777

END OF QUESTION PAPER

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