Densimat

DENSIMAT
SERVICE MANUAL
296.354
V.H 10.96
Australia
biohnrieux Vitek - Austraiia Pty. Ltd.
Unit 1,6 Gladstone Road
Castle Hill NSW 2154
tel. (61) 2.899 4600 -fax (61) 2.899 1232
Austria
bioMrieux Austria GmbH
Eduard-Kittenberger-Gasse 97
A-1230 Wien
tl. (43) 1.86 50 650 fax (43) 1.86 50 661
Belgium
bioMrieux Benelux s.a./n.v.
rue Victor Hugo 215 - 1030 Bruxelles
tl. (32) 2.743 01 70 -fax (32) 2.733 55 97
Brazil
Biolab Mrieux S/A
Estrada Mapua
491 Jacarepgua
CEP 22710 261 Rio de Janeiro R.J
tel. (55) 21.445 5454 - fax (55) 21.445 6099
Canada
bioMrieux Canada, Inc.
4535, Rue Dobrin
St Laurent - Qubec H4R 2L8
tel. (1) 514.336 7321 -fax (1) 514.336 6450
Chile
bioMrieux Chile S.A.
Av. Providencia 1945
Oficina 603 - Santiago Chile
tel. (56) 2.334 29 331334 15 93 -fax (56) 2.233 81 51
France
bioMrieux sa
69280 Marcy-lEtoile
tel. (33) 04 78 87 20 00 -fax (33) 04 78 87 20 90
Korea
bioMrieux Korea CO . Ltd
6th floor Duk Soo Building
234-9 Nonhyun-dong, Kangnam-ku Soul
tl. (82) 2.547 6262 -fax (82) 2.547 6263
Mexico
biohllrieux Mxico S.A. de C.V.
Av. Cuauhtmoc 1338 - Desps. 404 A y B
Col. Santa Cruz Atoyac - Delegacion Benito Juarez
C.P. 03310 Mxico, D.F.
tel. & fax (52) 5.604 02 43 5.605 80 24
The Netherlands
bioMrieux Benelux BV
Bruistensingel 620
5232 AJ s - Hertogenbosch
tel. (31) 73.6441818. fax (31) 73.6442211
Poland
biohllrieux Polska Sp. z 0.0.
ul. Zeromskiego 17
01-882 Warszawa
tl. (48) 22.663 4444 -fax (48) 22.669 99 42
Portugal
bioMrieux Portuguesa Lda
Calada de Santa Catarina, N 9 C
1495 Cruz Quebrada
tel.(351) 1.4 150278-fax(351) 1.41501 18
Russia
bioMrieux 0.0.0.
Mescheriakova 4,
Moscow
Tl. (7) 095.955 2627 / 235 5979 -fax (7) 095.955 2674
Spain
bioMrieux EspaAa s.a.
Manuel Tovar 36 - 28034 Madrid
tel. (34) 91.358 11 42 -fax (34) 91.358 06 29
Germany
bioMrieux Deutschland GmbH
Weberstrasse 8 - D 72622 Nrtingen
tel. (49) 7022.3007.0 -fax (49) 7022.36110
Hong Kong-China
bioMrieux Vitek - Hong Kong Ltd
Unit 1701-2, 17/F, Nanyang Plaza
No. 57 Hung TO Toad, Kwun Tong
Kowloon Hong Kong
tel. (852) 2356 7033 -fax (852) 2330 2085
Italy
bioMrieux Italia S.P.A.
Via G. Moscati 9 - 00168 Roma
tel. (39) 6.3014454 -fax (39) 6.3050079
Switzerland
bioMrieux Suisse s.a.
5l,avenueBlanc-1202Genve
tl. (41) 22.906 57 60-fax (41) 22.906 57 42
United Kingdom
biohllrieux UK Limited
Grafton Way, Basingstoke
Hampshire RC22 6HY
tl.(44) 1256.461881 -fax(44) 1256.816863
USA
bioMrieux Vitek, Inc.
595 Anglum Drive - Hazelwood
Missouri 63042 2395
tel. (1) 314.731 8500-fax (1) 314.731 8700
Japan
biohnrieux - Vitek Japan Ltd.
Otsuka HT Bldg.
43-1, Minami-Otsuka 3-chome
Toshima-ku, Tokyo 170
tel. (81) 3.5952 0821 -fax (81) 3.5952 0831
Distribution in over 130 countries
DENSIMATService Manual VA
Liability disclaimer:
A
BIOMERIEUX SA Will concede no formal or implicit warranty, as regards this Manual, its quality,
performance or appropriate use in any type of specific application.
This Manual cari be modified by BIOMERIEUX SA without notice, and represents no commitment
or Iiability whatsoever from BIOMERIEUX SA.
COPYRIGHT, BIOMERIEUX SA
BIOMERIEUX SA does in no case concede users of this Manual any right for reproduction,
adaptation or translation, by any process and in any country.
As the Law of March 1 lth, 1957 authorizes first copies or reproductions strictly reserved for the
private use of the copists and not intended for collective use and second, analyses or short
quotings intended for examples or illustrations, any representation or reproduction, whether
integral or partial made without the authors or owners consent is illegal. (Articles 40 and 41,
Law of March 1 lth, 1957).
,-
Such representation or reproduction, by any process whatsoever,*would therefore be considered

as a fraudulent imitation penalized by articles 425 etseq of the French Penal Code.
Table of Contents
List of Figures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
GENERAL CHARACTERISTICS
........................................
v-5
l-l
l-l
Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Environmental Conditions ..................................... l - l
Electrical Characteristics ...................................... l - l
Optical Characteristics ........................................ l - l
PRINCIPLE
.........................................................
UNPACKING AND REPACKING . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
l-3
2-l
Before Opening the Boxes: ................................. 2-1

When Opening the Boxes: ................................. 2-l
DENSIMATModule Packing List ................................ 2-l
INSTALLATION OF THE DENSIMATMODULE
..........................
2-3
DENSIMA T Module ............................................ 2-3

2-3
Position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
SECTION CONTENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
LayoutDiagram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Description of the Sensor Board .................................
Description of the Processor Board ..............................
Description of the Optical Block .................................
GENERAL DESCRIPTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Functional Description of each Card ........................
SensorBoard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Processor Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
OpticalBlock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
SENSOR BOARD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3-1
3-l
3-l
3-l
3-l
3-3
3-3
3-3
3-3
3-3
3-5
3-5
DESCRIPTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Preamplifier of Transmitted Light ......................... 3-5
Preamplifier of Scattered Light ............................. 3-5
Light Source Power Supply ................................ 3-5
Power Supply Micro Switch ............................... 3-5
3-5
OpticalInterrupt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
DENSIMAT Service Manual
V.A 10.96
V-l
PROCESSOR BOARD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
DESCRIPTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Processing of Transmitted Light ...........................
Processing of Scattered Light ..............................
Processing of the Battery Source ...........................
Management of the DENSIMAT . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Management with an External Computer ...................
Liquid Cristal Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Power Supply of the Numerical Part, . . . . . . . . . . . . . . . . . . . . . . . .
Power Supply of the Analog Part . . . . . . . . . . . . . . . . . . . . . . . . . . .
OPTICAL BLOCK . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
DESCRIPTION
3-7
3-7
3-7
3-7
3-8
3-8
3-8
3-9
3-9
3-9
3-11
..............................................
3-l 1
.........................
4-l
FIELD ENGINEER PREVENTIVE MAINTENANCE
Preliminary Procedures ........................................ 4-l

ScheduledProcedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-l
Yearly Preventive Procedure .............................. 4-l
CUSTOMER PREVENTIVE MAINTENANCE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
4-3
Preliminary Procedures ........................................

Scheduled Cleaning Procedures .................................
Clean the Photosensors every Month .......................
Additional Cleaning Procedure .................................
External Surfaces ........................................
4-3
4-3
4-3
4-3
4-3
TROUBLESHOOTING GUIDE
............................. ............
5-1
.............................
6-1
PREVIOUS INSTRUMENT VERSION TABLE
Management of Versions .......................................

Upgrading and Modification of DENSMAT . . . . . . . . . . . . . . . . . .
6-l
6-2
LIST OF SPARE PARTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7-1
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
List of Tools ...............................................
List of DENSIMAT Spare Parts .............................
V-2
7-l
7-1
7-l
7-l
DENSIMAT Service Manual V.A 10.96
BLOCK DIAGRAMS
.................................................
APPENDIX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
GLOSSARY
Appendix-1
.................................................
INDEX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
V.A 10.96
8-1
Glossary-1
Index-l
V-3
List of Figures
Figure
Description
Page
PRINCIPLE
Fig. 1.1
Fig. 1.2
Reading Principle of DEMIMAT

DENSIMAT Display Pane1
l-2
l-2
UNPAKING AND REPAKING

Fig. II.1
Front Pane1 of the DENSIMAT
2-2
GENERAL DESCRIPTION
Fig.
Fig.
Fig.
Fig.
Fig.
III.1
III.2
III.3
III.4
III.5
General Description
Sensors Circuit
Processor Circuit
Optical Block - Top View
Optical Block - Side Views
3-2
3-4
3-6
3-10
3-10
CUSTOMER PREVENTIVE MAINTENANCE

Fig. IV.1
Preparing the Cleaning Tool
4-2
SCHEMATICS DIAGRAMS
Fig. VIII- 1
Fig. VIII-2
Fig. VIII-3
-
General Interconnection
Processor Densitometer ASSY
Sensor Densitometer ASSY
V.A 10.96
8-3
8-5
8-7
V-5
CHAPTER 1
DENSIMAT is designed to measure the bacterial density in an ampoule of
liquid medium.
Dimensions
- Width
- Depth
- Height
- Mass
:
:
:
:
10.7cm
17cm
6.2cm
0.5kg
Environmental Conditions
- Indoor use
- Operating Temperature
- Relative Humidity
- Installation Category
- Pollution Degree
:
:
:
:
Max 30C
70%0
1 (see appendix J of EN 61010.1-1993)
2 (in accordance with EN 61010.1-1993)
Electrical Characteristics
- Battery Option
-Lithium battery LiSOC (3.6 V nominal size AA with a nominal
capacity of 1.8A/H)
- Adapter Power Supply AC Option
-Adapter Power Supply 23OV/6V - 9V 250mA for European
market
-Adapter Power Supply 115V/6V - 9V 300mA for Japanese
market
- Consumption : 1.3 A/H (Amperes x Hour)
-Automatic switching on when an ampoule is inserted into the optical
block.
Power Supply UL/CSA Code
- Input
: 115 VAC/GOHz USA STD Plug
- output : 7.5 VDC, 300mA with output connecter mod. PANASONIC
0=3.0xl.lmm
Power Supply complying with EN60742 Code
- Input
: 230 VAC/50Hz European STD Plug
- Output : 7.5 VDC, 250mA with output connecter mod. PANASONIC
0=3.0xl.lmm
Optical Characteristics
-
Light Source
Central Photosensor
Lateral Photosensor
Logarithmic display
in McFarland units
: LED 950nm
: BP 104 SIEMENS
: BP 104 SIEMENS
: 0.5 McF to 7 McF
l-l
Ampoule
~.-.-.~.~.~.-.-.-.~.~.~.~
Peripheral
Photosensors
-.-.-._._.-.-._._._. T:----- .-.-.-._._._._._.-.-.
1.
Central
Photosensor
-.-.-.-.-.-.-.-._._.*.
1.
Light source
1.
i._._._._._._._._LEP.-.-
RESULT PRESENTATION
Scale graduated in McFarland units
Fig. 1.1 - Reading Principle of DENSIMAT
McFarland scale
~~_~_~_~_~~~_~_~_
\
\
\.
0 . 5 -*
1 McF
0.4 -
Numerical
Value
-.-.-._._._.-._
Bar graph
The designated number is proportional to the value in McFarland units
Fig. 1.2 - DENSIMAT Display Pane1
l-2
V.A 10.96
PRINCIPLE
The DENSIMAT is a densitometer used to measure the density of a bacterial

suspension.
An incident beam of light is passed through the ampoule and two
subsequent measurements are obtained:
- Scattered light S (two photosensors)
- Transmitted light T (one photosensor)
The ratio S/T is directly proportional to the density of the bacterial

suspension.
The results are displayed on a mobile graduated scale on an LCD (Liquid
Crystal Display). The number of squares displayed is proportional to the
McFarland value.
,-
V.A Jo.96
l-3
CHAPTER 2
UNPACKING AND REPACKING
UNPACKING AND REPACKING
Before Opening the Boxes:
l Make sure that no damage has been caused during transportation.

If damage has occurred, make a claim to the transport company and notify
Customer Service at bioMrieux SA.
When Opening the Boxes:
l Make sure that there a11 the items listed on the packing list are included in
the boxes. If possible keep packaging materials in case the system has to
be moved in the future.
CA UTION!
Any damage directly or indirectly resulting from the

transport of the instrument without adequate
containers Will not be covered by the warranty or
maintenance contract.
DENSMAT Module Packing List

See the list included in the box.
DENSIMATService Manual V.A 10.96
2-l
1
I
--_-_-_---_---_--.--
.--_-__--------_-___-i----
Il
bidWrieux
Fig. 11.1 Front Pane1 of the DENSIMAT
2-2
DENSIMAT Service Manuai
V.A 10.96
INSTALLATION OF THE DENSIMAT MODULE
DENSIMAT Module
Position
l This instrument is designed to be used on a laboratory benchtop but may be
moved depending on the needs of the user.
oPlace the instrument on a flat, horizontal surface.
l The area should be free of dust. The room temperature and relative
humidity should be constant.
Do not place the instrument near strong sources of electromagnetic

interference.
V.A 10.96
2-3
CHAPTER 3
GENERAL DESCRIPTION
SECTION CONTENTS
This section includes:

Page
LAYOUT DIAGRAM
3-l
DESCRIPTION OF THE SENSOR BOARD
3-5
- Preamplifier of Transmitted Light

- Preamplifiers of Scattered Light
- Power Supply of the Light Source (LED)
- Power Supply Micro_switch
- Optical Interrupt
DESCRIPTION OF THE PROCESSOR BOARD
3-7
- Processing of Transmitted Light

- Processing of Scattered Light
- Processing of the Battery Source
- Management of the DENSIMA T
- Management with an External Computer
- Liquid Cristal Display
- Power Supply of the Numerical Part
- Power Supply of the Analog Part
DESCRIPTION OF THE OPTICAL BLOCK
3-11
- Optical Block
V.A Jo.96
3-l
OPTICAL BLOCK
LED 950nm
DC-PWR
SOCKET I
3-
-0
r33
Il
PHOTOSENSORS
SENSOR BOARD
-:. SP1 0
-:* SP9
r2;
it
____________
LITHIUM
BATTERIES
3.6 V nominal
PROCESSOR BOARD
Fig. III.1 General Descriplion
3-2
DENSIMAT
Service Manual
V.A 10.96
GENERAL DESCRIPTION
The real value of the bacterial density cornes from the calculation of the
ratio between the scattered light and transmitted light of the suspension.
The light source is an infrared source contained in the instrument.
The electronics of the DENSIMAT module are made up of two electronic
cards. These allow the space in the instrument to be optimized and possible
hanger-on with the signal to be decreased.
Functional Description of each Card

Sensor Board
- Light source power supply
-Micro_switch to switch the instrument ON/OFF
- Optical interrupt to Select the type of ampoule
- External mains outlet to connect the adapter AC power supply
Processor Board
- Amplifier circuit of photosensor signals.
- M i c r o c o n t r o l l e r c i r c u i t t o manage a11 the functions of the
instrument.
- Liquid Cristal Display (LCD).
- Components to generate the power supply from the batteries.
- Connecter to allow the link with a computer.
Optical Block
- Optical Block
V.A 10.96
3-3
CC
41,F
161
c* .
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4
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I UWLESS DTWERMSE IOTED ILL RESISTOR A R E
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NOTES:
Ponte a Ema FIRENZE
SENSOR BOARD
DESCRIPTION
The sensor board allows conversion of the light received on the
photosensors in proportional analogie signals.
- Preamplifier of Transmitted Light

The preamplifier circuit converts the current from the photosensor to a
voltage proportional to the ratio below.
Ratio = 100 mV / pA
- Preamplifier of Scattered Light

The preamplifier circuit converts the current from the photosensors to a
voltage proportional to the ratio below.
Ratio = 270 mv / pA
- Light Source Power Supply

The LED used as a power source should be power supplied with a constant
current.
For the components with U2, the constant current should be 76 mA
The switching ON/OFF of the LED is controlled by the logical level on the
3/U2 pin. The LED is ON when the logical level is 0.
The C7 capacitor decreases the stabilization time of the current in the
circuit.
- Power Supply Micro Switch

The SWl micro switch is an interrupt with contact no gift.
The plug for the external power supply is a coaxial plug with a BREAKBEFORE-MAKE switch to avoid loading the battery with current.
On the battery line the Dl Schottky diode limits the voltage on this line.
The Fl fuse (750mA 125V) protects the battery set.
The C6 capacitor is used as a regulator when the LED is switched ON.
- Optical Interrupt
This optical interrupt is used to identify the typeof ampoule when it is put
into the optical block.
This function is done with the OP1 optical interrupt.
TO reduce consumption, the optical interrupt uses an analogie OUTPUT.
V.A 10.96
3-5

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di
PROCESSOR BOARD
DESCRIPTION
- Processing of Transmitted Light
The processing of the signal cornes directly from the SENSOR board.
The processing is done through the U3 circuit section C. The amplifier
circuit is a noninverting operational amplifier.
The bandwidth is limited with a continuous theorical gain equal to 23.
The R23 resistor limits the current through the INPUT of the A/D
converter included in the microcontroller.
The Dl Schottky diode limits the voltage through the INPUT of the A/D
converter to VCC + 0.5V. It is necessary to avoid wrong converting of the
channel next to the converter, to make sure the voltage through the
INPUT does not exceed the reference voltage by more than one volt.
The signal of transmitted light goes directly to an INPUT of the A/D
converter through the R24 resistor, which limits the input current.
In this case, the Schottky diode is not required to protect the INPUT,
because the gain is 1 and it cannot exceed the reference voltage.
- Processing of Scattered Light

Processing of the signal cornes directly from the SENSOR board.
_-
The processing is done through the U3 circuit section B. The amplifier

circuit is a noninverting operational amplifier .
The bandwidth is limited with a continuous theorical gain equal to 2.5.
The D2 Schottky diode limits the voltage through the INPUT of the A/D
channel next to the converter, to make sure the voltage through the
Processing is done through the U3 circuit section A. The amplifier circuit
is a noninverting operational amplifier.
The bandwidth is limited with a continuous theorical gain equal to 23.
The D3 Schottky diode limits the voltage through the INPUT of the A/D
channel next to the converter, to make sure ,the voltage through the
V.A 10.96
3-7
- Processing of the Battery Source

This function allows you to check the battery voltage and display the level
of the battery.
Processing is done through the U3 circuit section D. The voltage of the
battery is divided between the two resistors R6 and R7 (7.2V nominal) to
adapt it to the characteristics of the A/D converter. The OUTPUT of the
amplifier serves as a buffer and the signal goes directly to an INPUT of the
A/D converter through the R29 resistor, which limits the input current.
If the battery voltage goes below 5.76 V the battery change indicator light
cornes on on the display.
-Management of the DENSIMAT

Management of the instrument is controlled by a single chip Ul
microcontroller. This circuit allows the following functions:
- Hardware control.
-Conversion of the analog signal from sensors into a numerical
signal.
- Reading management.
- Calculation and displaying of the result.
The microcontroller used is the single chip MC68HC711E9 from
MOTOROLA. Its characteristics are:
EPROM
EEPROM
A/D Converter
Input/Output
Clock
12 Kb
512 b
8 bits
5
8 MHz
Firmware
Values of calibration
With this microcontroller, the U2 circuit and the Cl4 Power-on Reset
capacitor allow the power supply level to be monitored. When the voltage
goes below 4.3V, the system generates a general reset.
Connexion with the LCD circuit is done through port B.
The high level of the PB3 line allows you to switch ON the light source
(LED).
The high level of the PB4 line allows you to manage the optical interrupt of
the type of ampoule.
The PAO line is used to read and manage information from the sensors.
- Management with an External Computer

Management with an external computer is done through the 52 connecter
Lines PB5 and PB6 are used during the processing of the adjustment.
3-8
V.A 10.96
- Liquid Cristal Display

The standard LCD component uses 16 ASCII characters per line on two
lines.
The LCD brightness is fixed by resistors R20 and R31.
The interface with the microcontroller is done to 8-bit MPU through PIA
(Port C) Signals PBO (Enable), PBl (Selects Read Write) and PB2 (Selects
Register) are used to control the OUTPUT.
- Power Supply of the Numerical Part

The power supply voltage VCC is produced using the U7 circuit and the
supply voltage obtained (5 VCC) is produced by the battery voltage.
- Power Supply of the Analog Part

The A/D converter circuit requires a voltage of 5000 mV.
This supply voltage is obtained from the U6 circuit.
The operational amplifiers require voltages at + 8 V ( +V) and -8 V C-V).
The supply voltage +V is obtained from the U4 circuit through the U6
circuit.
The supply voltage -V is obtained from the U5 circuit through the U4
circuit.
3-9
Fig. III- 4 Optical Block -Top View
-_-__-_-__-_
SP1
SP2
-_-__.
x
Fig. III.5 Optical Block - Side Views

3-10
DENSIMAT Service Manual V.A Jo.96
OPTICAL BLOCK
DESCRIPTION
The optical block is a mechanical block, where the LEDs and photosensors
are positioned to make nephelometric readings of an ampoule.
The LED is SFH400 III.
Photosensors are BP 104 SIEMENS
V.A 10.96
3-11
CHAPTER 4
FIELD ENGINEER PREVENTIVE MAINTENANCE
Preliminary Procedures
1) Make sure that the optical block is clean.
2) Check the external appearance of the DENSIMAT to detect any
damage.
Scheduled Procedures
Yearly Preventive Procedure
Materials used:
ATB ALIGNMENT KIT DENSIMATRef: 15512
[STEP 11
Clean the photosensors.

Using the cleaning tool and a sterile swab dampened with
alcohol, carefully clean the photosensors.
[STEP 21
Check the calibration.

Using ampoules from the kit, check the values obtained with
the DENSIMAT for each type of ampoule (large and small) and
compare them with values written on the ampoules.
CAUTION!
Depending on the results you should perform the

DENSIMAT calibration procedure.
4-1
,, A-/
,,Y
,f
Y II
- Cut the sterile swab to the right length

- Put the swab in the cleaning tool
- Dampen the swab with alcohol
Fig. IV.1 - Preparing the Cleaning Tool
4-2
V.A 10.96

CAUTION!
Disconnec;e;zemodule fro_m the external AC P~OT;~

performrng
marntenance
source
(Customer using ACAdapter).
Do not use thinner, trichloroethylene, or ketone-based
solvents on the plastic components of the module.
Preliminary Procedures
1) Make sure that the optical block is clean.
2) Check the external appearance of the DENSIMAT to detect any
damage.
Scheduled Cleaning Procedures

Clean the Photosensors every Month
Using the cleaning tool and a sterile swab dampened with alcohol,
carefully clean the photosensors located in the optical block.
Additional Cleaning Procedure

The following cleaning procedure is not included in the monthly cleaning
procedure. It is the operators responsability to decide how often it should
be performed.
External Surfaces
The external surfaces of the DENSIMAT should be cleaned with warm soapy
water to prevent the build-up of dust.
V.A 10.96
4-3
u
m
PREVIOUS INSTRUMENT VERSION TABLE

Management of Versions
The First Character M indicates that an instrument is being used (M:
Material, V: Software).
The Second C haracter gives information on the language being used

0 - Not used
1 - English
2 - German
3 - Italian
4 - Spanish
5 - German
6 - Portuguese
7 - French
8 - Multilingual
i- Frxh&English
B - French/English
C - Multilingual
D - English
X - Unknown
QWERTY
Qzz
$zERTY
QWERTY
AZERTY
AZERTY
QWERTY
AZERTY
QWERTY UK
The Third C haracter indicates the main voltage and the connecter
0 - Not used
1 - 220V / 50Hz
2 - 1lOV / 50Hz
3 - 90 to 240V / 50-60Hz
4 - 1OOV / 50Hz
5 - 220v / 60Hz
6-llOV/60Hz
7 - 90 to 240V / 50-60Hz
8 - 1OOV / 50Hz
9 - 240V / 50Hz
X - Unknown
European plug
European plug
European plug
European plug
us plug
us plug
us plug
Japanese plug
UK plug
The Fourth Character indicates the mechanical components design

1 - Original version
2 - Sequential progression
The Fifth Character indicates the electronics version

The Sixth C haracter gives the firmware version
The Seventh Character gives information on the software

6-l
Upgrading and Modification of DENSIMAT
Version
Upgrade
MO021 10
l-st version manufactured by bioMrieux

2-Mechanics
Supply with batteries and flags
1-Firmware
Original version : V 1 .l .O
6-2
Serial
Number
Spare
Parts ref
EXP
IDN 1001
V.A Jo.96
CHAPTER 7
LIST OF SPARE PARTS
LIST OF SPARE PARTS

Introduction
The list of spare parts is not exclusive, it only contains the main elements
of t he DENSIMA T.
Procedure
Al1 the way through this manual spare parts have been mentioned. They
are available from bioMrieux S.A.
A price list is established every year.
T O receive the prices, please contact the Field Technical Service at
biolllrieux S.A.
List of Tools
Reference
15512
Description
I DENSIMATATB ALIGNMENT KIT
4550389 A
DENSIMATATI KIT
4550409 A
Cleaning Tool
Comment
Version
I
I
DN0901-1
List of DEMIMAT Spare Parts

Reference
Description
Version
Comment
4500530 A
Optical Block
DNOlOl-1
4500532 A
PCB Densit Sensor Assy
DN8201-1
4521014A
I Finger
DN1003-1
4521015 A
Springs Finger
DN 1008-l
4500531 A
PCB Densit Processor Assy
DN8202-1
4521016 A
Top caver Assy
DN0102-1
4521013 A
Lower caver Assv
DNlOOl-1
7-l
CHAPTER 8
BLOCK DIAGRAMS
, OPTICAL Block
DNO101-1
LCD_DISPLAY
POHER IN
c 7-w +
SEWSORS ASSY
DM8201
CALIBRAlION POQT
3 FOR CAllEPAIIon PORT.
USE
SERIU
LOAPTLR
01(@251-1
ONCI
TOI SCHtYITIC DIACRW Of PRONSSOR ASSI SEE DOC NO DYBIOZ

2
1. fO1 SLWCYITIC DIASRW Of SEYSOR ASSI SEE BOL 10 DYBIOI
NOTES:
Ponte a Emo FIRENZE
4
Jl
Vi
=-"< PEZZI
OS1
l
Iln
c
03
Cl
\
03
---a
bioMriei;x Italia S.P.A.
UFF. DOCUfVENTAZIONE
UNLESS OTHERIISE NOTED, ALL INFORMATION

ARE FOR MANUFACTURJNG ONLY
PER L'APPLICAZIONE DELLA PkTA SALDANTE, USARE IL RETINO

SERIGRAFICO REALIZZATO CON LA MATRICE F ON8402-1 PU.
PER IL MONTAGCIO VEDERE DISTINTA BASE STESSO CODICE
Q
61
IL DISPLAY OS1 E' DA WERIRE CXIME ULTW CXBWNENTE, AVENDO

GLI SPILLI (POS. 3) CIA' SALDATI AL C.S. DELLA SCHEDA PROCESSOR
UONTARE IL CONNETTORE J2 DAL LATO SALDATURE
3
2
NOTE :
PER IL DISPOSITIVO U2, IN ALTERNATIVA A QUANT0 PREVISTO

A DISTINTA BASE, E' POSSIBILE UONTARE IL TIPO TL7757CD
PER LE SALDATURE USARE STAGNO 60-633

PER LO SCHEMA ELETTRICO VEDI DIS. ON8102
COMPLETARE 0 APPLICARE IL NUMERO 01 CODICE DOVE INDICATO
SEE SEPARATE 8. 0. Y.
FOR SCHEUATIC DIAGRAM SEE DN8102.
THIS ASSEMBLY CONTAINS ELECTROSTATIC DISCHARGE
HANDLE IN ACCORDANCE WITH PROCEDURE PO22022.
19-07~as
ACCIUNTO NOTE
FIRENZE
_
1
6.7.8 - PREPARAT0 PER EYISSIOH
biokieux Italia S.P.A.

Ponte 0 Em0
SENSITIVE DEVICES
II
ID. B.
1
I
PROCESSOR DENSITOMETER ASSY
FORMAT0
A3
Dis.N.
DN8202 A ,
TAVOCA
1 di 1
1 biollerieux I t a l i a S.p.A.A
osp9OSPI
5
A
Q
8
7
Q
Q
3
2
NOTE : A
PER IL MICROSWITCH SUI, IN ALTERNATIVA A QUANT0

PREVISTO A DISTINTA BASE, E' POSSIBILE MONTARE
IL 1'IPO FlT12.
PER L'APPLICAZIONE DELLA PASTA SALDANTE, USARE IL RETINO
SERI GRAFICO GENERAT0 CON LA MATRICE : F DN8401-1 PM Rev. A.
PER IL MONTAGGIO VEDERE DISTINTA
BASE STESSO KIDICE.

UFF. GCO_!F:!3lTAZIONE
UNLESS OTHERWISE NOTED

ALL INFORMATION ARE FOR MANUFACTURING ONLY
SEE SEPARATE B.O.M.
FOR SCHEMATIC DIAGRAM SEE DN8101.
THIS ASSEYBLY CONTAINS ELECTROSTATIC DISCHARGE SENSITIVE DEVICES
HANDLE IN ACCORDANCE WITH PROCEDURE PO22022.
PER IL Dl000 Dl, IN ALTERNATIVA A QUANT0 PREVISTO

A DISTINTA BASE, E' POSSIBILE MONTARE UN Dl000
IN CASE 00-41, Dl TIPO lN5818.
IL COMPONENTE Jl E' MONTAT0 DAL LATO SALDATURE.
MONTARE IL MICROSWITCH SU1 ED IL SENSORE OP1
PERFETTAMENTE A BATTUTA SUL C. S.
19-07-95
AGCIUNTO FUSIEILE FI. Dl000 Dl E NOTE 8. 9 - PREPARAT0 PER EYISSIONE
DATA
DESCRIZIONE YODIFICA
1
A
CI. 8.
ZONA
DIS
CONTR REV
PER LE SALDATURE USARE STAGNO 60-63%
DENSITOMETER ASSY
PER LO SCHEMA ELETTRICO VEDI DIS. DN8101.

COMPLETARE 0 APPLICARE IL NUMERO Dl CSDICE E SERIE
DOVE INDICATO.
A3
A
mammoau
bi
*
i
r
a
I
~
I
I
t
)
.
A
.
s
o
a
4
uI
a
mul
o
t
suun*
oau
mm
IL mxm mfw ma4 Mm*DN8201
txm
mumJc ~ILIZ ATO En IJ
1 di 1
Ponte a Ema
u SomlA
mamlnml~
FIRENZE
CeslmRIrn ca.Lm10
- , nnI
I@n w.t3m L E Dlg(SI#l ouu WICA
FORYATO
Dis.N
mAlo* w,m mumIcA10 A lmzl 0
olnllll A wow 31 LECQ
TAVOLA
nImmoTT0.
CHAPTER 10
GLOSSARY
Glossary
LED 950 nm
Light Emitting Diode 950 nm.

Light source emitting on the 950 nm wavelength.
Nephelometry
Measurement of the intensity of light scattered (S) at 30,

directly proportional to the bacterial growth.
McFarland
Standardization unit for measurement of bacterial

density.
Optical density
The result of the measurement of a medium through an

incident beam of light.
Photosensor
Sensor enabling a light source received to be transformed

into a source of electrical current.
Scattered Light
Light which is spread by the bacteria. In this case, the

light cornes out of the ampoule in a direction which is 30
from the direction of the emitted light.
Transmitted Light
Light which passes through the ampoule in the same

direction as emitted light.
DENSIMATService Manual
V.A 10.96
Glossary-1
CHAPTER 11
INDEX
Index
Page numbers in bolditalics correspond to figures or tables.
Ampoule,l-1, 7-2, l-3,3-3,3-5,3-8,3-11,4-1,

Glossary-1
McFarland, l-l, 7-2, l-3, Glossary-1

Medium, l-l, Glossary-1
Measure, l-l, l-3
Block, l-l, 3-1,3-2,3-3,3-5,3-10,3-l 1,4-l, 4-3
p
Photosensor, l-l, 9-2, l-3,3-2,3-3,3-5,3-1 1,41,4-3, Glossary-1
D
Density, l-l, l-3,3-3, Glossary-1
R
Reading, 3-8, 3-l 1
L
Light, l-l, 7-2,
Glossary-1
l-3,3-1,3-2,3-3,3-5,3-7,3-8,
S
Suspension, l-3,3-3
V
Value, 7-2, l-3,3-3,3-5,3-8,4-1
Index-l

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DENSIMAT

Distribution in over 130 countries

Such representation or reproduction, by any process whatsoever,*would therefore be considered

UNPACKING AND REPACKING . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Before Opening the Boxes: ................................. 2-1

DENSIMA T Module ............................................ 2-3

FIELD ENGINEER PREVENTIVE MAINTENANCE

Preliminary Procedures ........................................ 4-l

Preliminary Procedures ........................................

PREVIOUS INSTRUMENT VERSION TABLE

Management of Versions .......................................

LIST OF SPARE PARTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

DENSIMAT Service Manual V.A 10.96

DENSIMAT Service Manual

Reading Principle of DEMIMAT

UNPAKING AND REPAKING

Front Pane1 of the DENSIMAT

CUSTOMER PREVENTIVE MAINTENANCE

Preparing the Cleaning Tool

DENSIMAT Service Manual

DENSIMAT Service Manual V.A 10.96

Scale graduated in McFarland units

Fig. 1.1 - Reading Principle of DENSIMAT

Fig. 1.2 - DENSIMAT Display Pane1

DENSIMAT Service Manual

The DENSIMAT is a densitometer used to measure the density of a bacterial

The ratio S/T is directly proportional to the density of the bacterial

DENSIMAT Service Manual

UNPACKING AND REPACKING

Before Opening the Boxes:

l Make sure that no damage has been caused during transportation.

When Opening the Boxes:

Any damage directly or indirectly resulting from the

DENSMAT Module Packing List

DENSIMATService Manual V.A 10.96

Fig. 11.1 Front Pane1 of the DENSIMAT

DENSIMAT Service Manuai

INSTALLATION OF THE DENSIMAT MODULE

oPlace the instrument on a flat, horizontal surface.

Do not place the instrument near strong sources of electromagnetic

DENSIMAT Service Manual

This section includes:

DESCRIPTION OF THE SENSOR BOARD

- Preamplifier of Transmitted Light

DESCRIPTION OF THE PROCESSOR BOARD

- Processing of Transmitted Light

DESCRIPTION OF THE OPTICAL BLOCK

DENSIMAT Service Manual

Fig. III.1 General Descriplion

Functional Description of each Card

DENSIMAT Service Manual

6 FOR ASSfYBLV SEE D O C

5 . CDNNECT ALL CROUYDS AT S O L D E R POlNI O F BAlTERI.

NL IIOY-FDLIRIZEO CAPACITORS ARE CERAYIC WLTILAVER

C A P A C I T O R S AM SOL10 TAUlMIUY ELEClRDLIlIC 1TPE.

2 . UIILESS DIIIERUISE WOTED U L CAPA(IIAYCE V A L U E ARE Ill YICRDFAAAD

O H M . SS. l/lOW. ~OUF,.

- Preamplifier of Transmitted Light

- Preamplifier of Scattered Light

- Light Source Power Supply

- Power Supply Micro Switch

bioMrieux Italia S.P.A.

VMIATO VYORE bI RIO bA IW A 1%

IRVQlITf MASSE :AClID E DCUO