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Widad Mustafa Al-ani
Al-Mustansiriya University
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ISSN 0976-8157
Research Article
PHARMACIE GLOBALE
INTERNATIONAL JOURNAL OF COMPREHENSIVE PHARMACY
ABSTRACT
Melilotus officinalis, a plant belong to the family fabaceae. It is characterized by a particularly wide spectrum of
different plant constituents. Many compounds have been isolated and identified from the plant. These included
coumarins, flavonoids, essential oils, vitamin C, allantoin, and tannins. Coumarins are the major constituent of
melilot they are blood thinner used to keep blood flowing smoothly and prevent the formation of blood clots. In this
study coumarin was isolated from melilotus officinalis widely grown in Iraq by column chromatography. The
isolated coumarin was identified by measuring its melting point, GC/MS, TLC, HPTLC and UV spectroscopy.
Preparative TLC was performed to measure the percentage of coumarin in the Iraqi plant.
Keywords: Melilot; Coumarin; Isolation.
INTRODUCTION
GC-MS
(Shimadzu),
preparative
thin
layer
chromotagraphy HPTLC (CAMAG), IR, UV spectroscopy. All
chemicals and solvents are of analytical grade. Standard
coumarin was obtained from Sigma Aldrich Company.
Plant material
The aerial parts of Melilotus officinalis were collected from
area Abu-Graib in Baghdad and authenticated by the
National Herbarium at Abu-Graib, they were dried in
shade for several days at room temperature and then
grinded as powder.
Extraction and Isolation of Coumarin
Powdered plant aerial part (50g) was extracted by Soxhlet
apparatus with ethanol (80%, 250mL) till exhaustion. The
extract was concentrated by evaporation under vacuum.
Water (100g) was added and the suspension was
partitioned with petroleum ether (2x 100ml). The pet
ether layer was discarded and the aqueous layer was
extracted with ether (3x100ml). The combined ether
layers were dried with anhydrous sodium sulphate,
filtered and evaporated under vacuum. The residue
purified by column chromatography (silica gel, DCM
Pharmacie Globale (IJCP), Vol. 05, Issue 02
Figure 5. UV spectra
Thinlayer Chromatography
The Rf value of isolated coumarin was compared with
standard coumarin in three solvents system. Preparative
TLC was performed using 0.5 mm thickness silica gel.
Elution of the isolated band was conducted with AR grade
acetone.
Rf of standard
coumarin
Rf of isolated
coumarin
0.65
0.64
0.55
0.54
0.3
0.29
CONCLUSION
REFERENCES