Beruflich Dokumente
Kultur Dokumente
Department of Zoology, Nnamdi Azikiwe University, PMB 5025, Awka, Anambra, Nigeria .
2
GreenPlanet Integrated Resources, Lagos, Nigeria
*Corresponding Author: cd.ezeonyejiaku@unizik.edu.ng
INTRODUCTION
The risk posed by the atmospheric environment to both living and non-living
organisms is astronomical compared to other environmental media. The quality of
the air medium is a function of the activities operating within the confinement of
that air irrespective of the exposure ratio. Atmospheric heavy metal deposition
using on-site environmental biomonitor is now gaining relevance in Nigeria due to
the natural selection and ubiquity of the organisms. In a previous study (in press),
we demonstrated heavy metal concentration measurements based on lichen
mapping and sampling.
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population of over 37,000 at its over 100 acre main campus. The University ranks
among the top 10 universities in Nigeria in research output.
The entire university was mapped for the study. It was divided to cover all the
schools and faculties. The sampling locations were allocated to cover the science
village, School of Management and Social Sciences, and then the Administrative
block as shown;
Location 1: Science village
Location 2: Environmental sciences
Location 3: school of social sciences
Location 4: Administrative block
Prior to the collection and sampling of lichens, earlier reconnaissance tour was
embarked on mapping the lichen diversity at the university surrounding (data not
shown). This was done to select the suitable and widely distributed species for
effective monitoring. The substrate of the lichen species were also put into
consideration as this influences their metal surface absorptions. The fruticose
lichen diversity was very low for the study and the few located were at the forest
cover area of the university. Foliose-type of lichens was widely distributed and was
recruited for the study. Lichens of Parmeli family were used for the entire
experimental investigations.
The lichen samples preparation and analysis were evaluated based on the
modified method earlier adopted from Kapu et al. 6 and used for similar studies.
6g of the lichen samples were dried to constant weight at 600C in a hot air oven
and cooled in desiccators. The dry material (3g) was then ashed in a muffle furnace
for 6 hrs at 4600C, and the ash was cooled in desiccators for 24 hrs. The ashed
samples were digested for 3 hrs on a hot plate at 850C using 20ml of a 1:1 (w/v)
hydrochloric acid: water mixture. The digests were extracted using deionized water
and filtered into 25ml polyethylene bottles. The concentrations of Hg, Pb, Zn, Cr,
Cu, Cd, and as (ppm) were determined triplicate by atomic absorption
spectrophotometer (Perkin model). Standards containing known amount of these
metals and blank samples were subjected to the same procedure to estimate
recovery rates and to check for contamination. The average recovery was 96-97%.
Standard solutions of metals were prepared by dilution of 1000 mg/L.
RESULTS
The concentrations of Pb, As, Cd, Cu, Cr, and Zn are presented in Table 1. There
was variable distribution in the concentrations of the heavy metals over the
sampled location sites. The lichen samples were observed to have accumulated
highest (P<0.05) concentrations of as in their tissues. Hg was not detected from all
the locations. Location 2 showed highest (P<0.05) concentration of Zn over other
locations sampled.
Table 1. Concentrations of heavy metals observed in the foliose-type of lichens at
the study area
Locations
0.086a
0.013
0.330b
0.150
1.239c
0.460
1.878c
0.648
Zn
+
+
+
+
1.862a
0.286
5.943d
0.275
0.556b
0.144
2.945c
0.517
Cr
Cu
ND
ND
0.123
0.050
0.186
0.051
0.164
0.056
0.166
0.050
0.151
0.052
0.186
0.048
+
+
Cd
+
+
+
+
1.173c
0.039
0.170b
0.019
0.065a
0.026
ND
As
+
+
+
23.523c
0.450
17.398b
0.449
0.282a
0.058
0.306a
0.132
+
+
+
+
Means with the same superscripts along the same column are significantly
different (P<0.05(.
ND: Not Detected
DISCUSSION
Basis on the spatial data of heavy metal concentrations in epiphytic lichen
species, the atmospheric environment of the university is considerably laden with
pollutants of significant risk. The quantities of heavy metals observed are
appreciable taking into account the medium under study and the environment of
presence. One possible explanation could be the longevity of lichens compared to
plants, which make them intercept and absorb pollutants for a longer period and
reflect the pollution history of the environment over time. Similar stand had been
maintained by Augusto et al. 12.
Variations observed in the concentrations of the heavy metals measured in the
lichens may be related to numerous factors. As observed in our previous studies,
similar variation was also found. Air pollutants have a different effect on the fungal
and other the algal partner of the lichen species (symbionts). The algal partner has
been reported to react more sensitively, e.g., to acidic deposition and heavy metals,
and to show varying accumulation of metals depending on the acidity of
precipitation 2. Sporadic desiccation of lichens may also have an effect on the
accumulation and absorption of elements 13, resulting in the observed random
variability of the heavy metal concentrations sampled. Supporting the variation
hypothesis could be the reports of Bargagli 14 and Bargagli et al. 15, which
emphasized that after a dry period, rainfall may result in appreciable washing off
particles and the exchange of cations bound on negatively charged exchange sites
on the cell walls and plasma membranes of the cells. Comparing the statement to
the current findings, the sampling was done during the highest peak of rainfall
season (July).
The higher accumulation of Zn compared to others could be related to the age
of the lichens. As earlier quoted that lichen has long period of survival and the older
parts carry fruiting bodies rich in metals 2. Alternatively, while comparing lichens
and mosses element retention capacities, Hoodaji et al.
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tend to retain higher values of Zn and Hg concentrations than mosses. The fact we
did not detect Hg maybe there is no source of the metal around the area or the
metal naturally become disposed and off the detection limit. Different heavy metal
concentrations have been reported by Adamo et al. 7 while working on lichens,
Pseudevernia furfuracea and foliose type, Parmelia and Hypogymnia. Synonymous
findings have been documented by Conti et al. 8 in Evernia prunastri, NG et al. 9 in
Dirinaria picta, Cayir et al. 10 in C. rangiformis and Rajesh et al. 11 in Pyxine cocoes
and Phaeophyscia hispidula. However, the heavy metal concentrations (Pb, Zn, Cr,
and Cu) observed in the current study were lower than the values of similar metals
documented by the above authors in different lichen species 7-11 except Cd
concentrations in location 1, which were higher than those recorded by Adamo et
al. 7, Conti 8 and NG et al. 9.
Arsenic (As) is a ubiquitous element and it ranged from 0.282 + 0.058 to 23.523
+ 0.450 ppm in the air as obtained from the lichen samples. Maximum amount of
as was reported from location 1 (Science village) and 2 (Environmental Sciences),
which are more close to the traffic highway and road networks. Location 1 is few
metres away from the traffic highway and has linear proximity to the semiindustrial area of Okpuno, Awka. Vehicular emissions and industrial influences may
attest the elevated as quantity above background concentration. Earlier studies at
this Okpuno Awka indicated high as concentrations within the surrounding air.
Moreover, location 2 with the highest as might be under the influence of aerially
dispersed vehicular emissions from the road networks within and outside the zone
and industries. Arsenic (As) is released unintentionally as a result of many human
activities like smelting, industrial applications and with combustion of fossil fuels.
Rapid leaching of as has been observed in case of exposed wastes of ores and
mining, as well as soil erosion 16, 17. In this study, maximum as 23.523 + 0.450 ppm
was reported from location 1 (Science village). In an earlier study Rajesh et al. 18, 19,
reported maximum concentration of 51.90.1 g g-1 DW of As in thallus of
Phaeophyscia hispidula from a sites having past mining activities.
The study indicates that heavy metal concentrations in the air may have greater
effect than demonstrated, reaching up to the food chain. The use of lichen species
in the investigation has shown once again its effective recruitment in predictive
and on-site atmospheric monitoring of contaminants and identification of
potential hazards. Arsenic concentration observed in the study is of great concern
as it poses a great risk of carcinogenicity. It is important to monitor the As in Awka
on a wide scale to better understand the point sources and environmental
distributions. It is also crucial to understand the mechanisms of as uptake,
translocation and transformation in lichens. Research should be embarked to
ascertaining the immediate risk to the population living in the vicinity of the study
area, long term hazard due to metal accumulation should be seriously considered.
Appropriate and functional environmental plan should be initiated and sustained
to reduce pollution and/or environmental contamination. This study having shown
the ambient air quality based on lichen survey would provide a platform on which
to launch subsequent research on atmospheric environment.
REFERENCES
1. Nash, T. H., ed. (2008). Lichen Biology (2nd ed ed.). Cambridge University Press.
pp. 56. ISBN 978-0-521-69216-8.
2. Hoodaji, M., Ataabadi, M. &Najafi, P. (2012). Biomonitoring of airborne heavy
metal.
3. contamination, in Air pollution - Monitoring, Modelling, Health and Control, Dr.
Mukesh Khare (Ed.), ISBN: 978- 953-51-0381-3, InTech, Available from:
http://www.intechopen.com/books/air-pollution-monitoring-modellinghealthand-control/biomonitoring-of-airborne-heavy-metal-contamination
4. Wolterbeek, H.Th., Garty, J. Reis, M.A. & Freitas, M.C. (2003). Biomonitors in Use:
Lichens and Metal Air Pollution, In: Bioindicators and Biomonitors, B.A. Markert,
A.M. Breure, & H.G. Zechmeister, 377-419, Elsevier, ISBN 0080441777, Oxford.
5. Szczepaniak, K. & Biziuk, M. (2003). Aspects of the biomonitoring studies using
mosses and lichens as indicators of Metal Pollution. Environmental Research, 93
(3), 221230.
6. Bowen, H.J.M. (1979). Environmental Chemistry of the Elements, Academic
Press, ISBN 0121204502, New York.
7. Kapu, M.M., Ipaye, M.M., Ega, R.A.I., Akanya, H.O., Balarabe, M.L., and Schaeffer,
D.J (1991). Lichens as biomonitors of aerial fallout of heavy metals in Zaria
Nigeria. Bull. Environ. Contam. Toxicol., 47: 413-416
8. Adamo, P.; Giordano, S. Vingiani, S. Cobianchi, R.C. & Violante, P. (2003). Trace
element accumulation by moss and lichen exposed in bags in the city of Naples
(Italy). Environmental Pollution, 122 (1), 91103.
9. Conti, M.E.; Tudino, M. Stripeikis, J. Cecchetti, G. (2004). Heavy metal
accumulation in the lichen evernia prunastri transplanted at urban, rural and
industrial sites in central Italy. Journal of Atmospheric Chemistry, 49 (1-3), 83-94 .
10.
NG, O.H.; Tan, B.C. & Obbard, J.P. (2006). Lichens as bioindicator of
atmospheric heavy metal pollution in Singapore. Environmental Monitoring and
Assessment, 123(1-3), 63-74.
11.
Cayir, A.; Coskun, M. & Coskun, M. (2007). Determination of atmospheric
heavy metal pollution in Canakkale and Balikesir Provinces using lichen
(Cladonia rangiformis) as a bioindicator. Bulletin of Environmental
Contamination and Toxicology, 79 (4), 367370.
12.
Rajesh, B., Pandey, A.K., Debba, F., Upreti, D.K., Nayaka, S., & Pandey, V
(2011). Physiological effects of arsenate on transplant thali of the lichen Pyxine
cocoes (sw.)Nyl. Environ. Sci. Pollut. Res.
13.
Augusto, S., Pinho, P., Branquinho, C., Pereira, M.J., Soares, A., and Catarino,
F. (2004). Atmospheric dioxin and furan deposition in relation to land-use and
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