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Malithi Weerakkody
Member
06-30-2013, 12:59 AM (This post was last modified: 06-30-2013, 01:07 AM by Administrator.)
26 Posts
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#1
Although not so widespread as single cell proteins, microbial production of oils and fats, termed as
Single Cell Oils (SCO), is a concept that is becoming increasingly popular among the scientists and
industrialists. These microbial oils can be used for a variety of production lines ranging from edible oils
and fats to biodiesel. Whereas the current trends of research in SCO mostly concentrate on the
production of biodiesel, this article focuses on the production of edible oils and fats of microbial origin.
Oleaginous Microorganisms
Although all microbes have the ability to produce some amount of lipids for their structural
components such as membranes, only a few of them are able to accumulate lipid in amounts which are
of commercial importance. Those microorganisms which have the ability to amass lipids more than
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The synthesis of lipids by microorganisms has several advantages over the production of plant or
animal derived oils. Microbes have shorter life cycles than plants or animals, warranting a rapid
production. Unlike other sources, there is no requirement for farm lands and therefore the eect of
climatic factors on the production is insignificant. Moreover, microbial fermentation involves less
labour and the scaling up the production is easier than in other methods.
On the other hand, the microbial synthesis of lipids is not as economical as the plant oil production due
to the high cost of the carbon sources and the requirement of sophisticated extraction techniques.
Furthermore, the oil yields which can be obtained by microorganisms are comparatively lower than
that with plants or animals.
However, these organisms can be genetically engineered to utilise cheaper substrates such as industrial
wastes. Another more economical approach is modifying these organisms into producing more valueadded specialty fats and oils or products which cant be extracted through other sources. This will be
more profitable than modifying plant oils into products of higher value such as cocoa butter.
Single Cell Oils in the Market
An infant formula including a blend of single cell oils, namely arachidonic acid (ARA) and
docosahexaenoic acid (DHA) is currently available in Europe, Australasia, Far East and USA. In addition,
cocoa butter-like products are produced using several species of yeasts such as Cryptococcus curvatus.
Furthermore, many polyunsaturated fatty acids (PUFA) which have gained interest as dietary
supplements and nutraceuticles, are being synthesised using microorganisms, mostly fungal and algal
species.
Sources:
Biotechnology for the Oils and Fats Industry edited by Colin Ratledge, Peter Stephen Shevyn Dawson,
James Rattray
Food Biotechnology (2nd edition) edited by Kalidas Shetty, Gopinadhan Paliyath, Anthony Pometto and
Robert E. Levin
Quote
debram
Senior Member
10-01-2014, 02:23 PM
47 Posts
1
#2
The edible oils extracted from microorganisms that are single-celled entities present at the bottom of
the food chain are known as single cell oils. Dierent types of fungi and yeasts are the best producers
with highest oil contents followed by various key algal groups with the capability of producing high
levels of PUFA with nutritional significance. The potentiality of algae to produce PUFA has currently
galvanized the interest in these fatty acids enriched single cell oils essential for the well being of
mankind that are actually not available from plants or animals.
Although potential producers of single cell oils, opportunities for the biotechnological exploitation of
the microorganisms are yet limited to the high-value products. Recently an attempt to produce a
species of yeast, oil equivalent to cocoa-butter did not materialize due to a reduction in the price of
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cocoa butter in the world market and not due to inability of development of correct formulation of fatty
acids. Production of PUFAs of both the n-3 and n-6 series from bacteria, fungi or algae are gaining
better prospects in recent times. It is also observed that certain microbial PUFAs are devoid of other
PUFAs, making the purification of desirable fatty acids quite easy than from other sources. Some
microorganisms also generate precursors of prostaglandin and also prostaglandins themselves along
with cerebroside lipids that are not usually regarded as of microbial origin.
Various microorganisms now have evolved to produce SCO as the commercial source of
docosahexaenoic acid (DHA) and arachidonic acid (ARA). SCOs are now extensively used as dietary
supplements in infant products. To improve lipid yields a better understanding of genetics and
biochemistry of oil accumulation in microorganism is quite essential. Also an understanding of the
mechanism involved in the polyunsaturated fatty acids (PUFAs) production is also highly desirable for
increasing the oil content. A report on the biosynthetic machinery revealed that oil accumulation in
oleaginous species accounts of lipid build up to more than 70% of the cell biomass. Whilst most of the
microorganisms use a conventional fatty acid synthase (FAS) pathway for production of
polyunsaturated fatty acids (PUFA) followed by a series of elongases and desaturases., it now appears
to be via a polyketide synthase (PKS) system in Schizochytrium sp and probably in related
thraustochytrid marine protists. It clearly represents a major deviation from the conventional
mechanism of fatty acid biosynthesis, possibly as a way to decrease the amount of NADPH needed in
the overall production process.
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