Result and Calculation

Sample source : Water from G3 lake

PH value : 6.59

BOD ID

Volume of
sample (mL)

Volume of
Dilution water
(mL)

Initial DO
(mg/L)

Final DO
(mg/L)

Sample 1
Sample 2
Sample 3
Blank 1
Blank 2

2
2
2
0
0

298
298
298
300
300

8.07
8.07
8.08
8.04
8.04

7.23
7.46
7.63
8.04
8.04

Average initial DO and Final DO of sample,

Initial DO =

Final DO =

8.07 8.07 8.08

8.07mg / L
3
7.23 7.46 7.63
7.44mg / L
3

Eqn A = minimum waste to be filled in BOD bottle

= [ ( 2 mg/L ) * 300 mL ] / 333 mg/L
= 1.80 mL
Eqn B = maximum waste to be filled in BOD bottle
= [ ( 8 mg/L 1 mg/L ) * 300 mL ] / 333 mg/L
= 6.30 mL

Data Analysis

1. Calculate the BOD

BODX mg/L =

DiDf
P

Where ,
Di = Initial DO ( mg/L )
Df = Final DO ( mg/L ), 5 days or 3 days
P = Volume of sample used / Total Volume
P = 2 ml / 300 ml
= 0.006
BOD ID

Value of BOD3

Sample 1

8.07 7.23
(2 300)
BOD3 mg/l =

= 126 mg/l

8.07 7.46
(2 300)

Sample 2
BOD3 mg/l =

8.08 7.63
( 2 300)

Sample 3
BOD3 mg/l =

Average =

= 92 mg/l

= 68 mg/l

126mgL 92mgL 68mgL

95mgL
3

2. In your opinion, is it possible to discharge the sample used in this test to be

discharged to the river? Give your reason.
According to the Sewage/Effluent Standard (DOE, Malaysia), the sample cannot be discharge
to the river untreated because the value obtained with 2 ml of wastewater is higher than 50
mg/L.