Beruflich Dokumente
Kultur Dokumente
RESEARCH ARTICLE
Department of Microbiology, Adhiparasakthi College of Arts and Science, G.B.Nagar, Kalavai, Tamil Nadu, India
*Correspondence to: E-mail: pringale@gmail.com; Phone: 91-7829014014
Abstract
Bacteriocins obtained from lactic acid bacteria (LAB) in milk samples have found commercial applications in the food processing industry
as a biopreservative or probiotic. Donkey milk has recently found application as a safer substitute to human milk. Lactobacillus paracasei
was isolated from donkey milk. Crude bacteriocin was separated from the culture as cell-free supernatant. The crude bacteriocin was
subjected to antimicrobial activity against clinical pathogens. In the present study, Maximum inhibitory activity was observed against
Salmonella typhi followed by Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Minimum inhibitory concentration
(MIC) was also performed and the MIC index of 128 l was seen against Salmonella typhi. Staphylococcus aureus showed the least
susceptibility to the crude bacteriocin. The study suggests that the crude bacteriocin from Lactobacillus paracasei could be used as a
potential probiotic or a bio-preservative.
Keywords: donkey milk, bacteriocin, Lactobacillus paracasei, antimicrobial susceptibility, minimum inhibitory concentration (MIC)
Received: 09th October; Revised: 25th October; Accepted: 02ndNovember; IJCS New Liberty Group 2011
Introduction
at
the plates, and were incubated at 37C for 24 hours and activity
37C
for
24-48
hours.
After
Incubation,
bacterial
was observed.
Identification
Optimization assay
6.0, 7.0 and 8.0 and NaCl concentrations 0.5%, 1%, 1.5%, 2%,
2009).
Purification of bacteriocin
Result
Arabinose
Negative
Mannose
Positive
Fructose
Positive
Rhamnose
Negative
Results
Cellobiose
Positive
Ribose
Positive
Galactose
Positive
Trehalose
Positive
Glucose
Positive
Characteristics
Result
Colony Morphology
Colony Size
2-3 mm
pH
7.0
Temperature
Anaerobic, 37C
Morphology
Rods
Gram strain
Gram positive
Motility
Non-motile
2500
2000
1500
1000
500
0
20C
30C
40C
50C
60C
Tem perature
3000
Bacteriocin Prodiuction
(Au/ml)
Lactobacillus paracasei
Bacteriocin Production
(AU/ml)
2500
2000
1500
1000
500
0
4
pH
Catalase
Negative
Oxidase
Negative
Indole
Negative
Methyl red
Negative
Voges proskauer
Negative
Citrate Utilization
Negative
3000
Bacteriocin Production
(AU/ml)
Biochemical Test
2500
2000
1500
1000
500
0
0.5
1.5
2.5
Degree of Inhibition
Escherichia coli
+++
Klebsiella pneumoniae
+++
Pseudomonas aeruginosa
+++
Salmonella typhi
+++
Staphylococcus aureus
++
Protein estimation
Fig. 3a. The optical density (O.D) at 600 nm for protein content
isolates
Clinical
1024
512
256
128
64
32
16
MIC
512
256
256
128
512
MIC assay
aeruginosa
and
Klebsiella
pneumonia,
512
against
Gram-positive
and
Gram-negative
bacteria
Discussion
isolation,
species have been isolated and studied previously from cow and
The
present
investigation
highlights
the
56.
in this tube.
91-142.
J 4: 43-47.
215-22.
avenues.
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