Beruflich Dokumente
Kultur Dokumente
AY 2016-2017
Prepared by:
GROUP 4
SULO, Ericsson C.
TADLE, Marjorie Hayes D.G.
TOLENTINO, Celine E.
VARGAS, Louise Erika Z.
VILLANUEVA, Matthew L.
3Chemistry
I. Introduction
Ultraviolet-visible spectroscopy or UV-Vis is a very vital part in the quantitative
analysis of liquid solutions. The UV-Vis spectrometry is known for being efficient,
flexible, and cost-effective. One of its uses is regulatory testing. It is one of the chosen
mode of analysis because of the information that it can provide.
The principle behind the UV-Vis spectroscopy is the UV-Vis spectrum. UV-Vis
spectroscopy uses the concept of the electromagnetic spectrum, mainly the ultraviolet
and visible region. The wavelength range of this spectroscopy is 200-380 nm (near
ultraviolet) and 380-780 nm (visible). The visible spectrum pertains to the different
colors and the corresponding wavelength range. Some of organic molecules undergo
electronic transitions upon interaction with the types of wavelength mentioned making
an electron to jump from a lower energy to a higher. Shorter wavelengths corresponds
to higher energy radiation.
The types of analytes that are subjected to analysis are but not limited to metal
ions, organic compounds with conjugated pi electrons and other biological organic
compounds. All samples must be in the form of solution and must fall into the
specification of the wavelength range. Common samples are dissolved in solvents like
water, ethanol and hexane. Concentration of species can also be identified using this
spectroscopy. For solutions containing metal ions, the response of the instrument is by
absorbance meaning if the concentration increases, the absorbance also increases
having a directly proportional relationship between the two values. The relationship of
A=log
( PP )=abc
0
Source: https://upload.wikimedia.org/wikipedia/commons/9/95/Schematic_of_UV-_visible_spectrophotometer.png
Start-up procedure
A. Instrument initialization
1. Turn the instrument and the computer on.
Shut-down procedure.
2. Be sure that the UV-Vis spectrometer is close and turn it off before unplugging.
3. Shut-down the computer used in the analysis.
iii.
Precautions/ Hazards
1. Do not place anything on top of the instrument. Be sure that the solutions used
will not spill over the instrument.
2. The cuvettes must not be over or under filled.
3. Be sure the cuvettes are clean before and after the analysis.
4. During the sample run, the cuvettes should be clean in the opaque side and must
not be wet.
5. Instrument must be closed in the sample running.
6. Be sure to check if the computer and instrument used are properly shut down.
V. References
1. GE Healthcare Life Sciences. Spectrophotometer Health and Safety Document
including
General
Operating
Instructions.
https://promo.gelifesciences.com/gl/spectrophotometers/misc/Health_and_Safety_m
anual.pdf
2. Perkin
Elmer
Inc.
Lambda
25,
35,
45:
Users
guide.
http://people.bath.ac.uk/gp304/uv/PerkinElmer_Lambda35_manual_EN.pdf
3. Sheffield Hallam University. UV-Vis Absorption Spectroscopy: Theoretical principles.
http://teaching.shu.ac.uk/hwb/chemistry/tutorials/molspec/uvvisab1.htm
4. William
Reusch.
Visible
and
Ultraviolet
https://www2.chemistry.msu.edu/faculty/reusch/virttxtjml/spectrpy/uvvis/spectrum.htm
Spectroscopy.