Beruflich Dokumente
Kultur Dokumente
Group 6
Pharmacy
ABSTRACT
Chromatography is a physical method that is used in the separation and analyzation of components in a mixture. The
experiment aimed to determine the purity and Retention factor value of the colored pigments through Thin Layer
Chromatography (TLC). In this experiment, pigments of malunggay were extracted and colored eluate was collected
through the Column Chromatography method. Afterwards, the purity of the components were determined through the
TLC method, and the developed TLC plate was used in order for the Retention Factor value to be measured.
INTRODUCTION
Chromatography is a technique that is
widely used for identifying and analyzing
mixtures through the separation of various
chemical
compounds.
Different
types
of
chromatography are very useful for analytical
purposes, depending on the physical state of the
phases used. In this experiment, Column
Chromatography was used to separate the
organic
compounds
of
malunggay.
The
components were distributed in a stationary and
mobile phase and were carried along to each
phase in varying degrees. The column was not
allowed to run dry as the eluates were collected.
EXPERIMENTAL
The compound tested was malunggay and
after it underwent through the process of column
chromatography, 4 eluates were yielded from the
extraction. Yellow, Green, Light Green, and Light
Yellow were extracted respectively; also, the
number of drops per color was noted.
RF Value
The
compounds
extracted
from
the
malunggay
leaves
were
colored,
thus,
its
visualization can be straightforward. UV lamps are
used to visualize the colorless compounds on the
plate. Also, the thin layer plate has a substance
added to it that will fluorescence when exposed to
UV light. Thus, making it glow under a UV lamp.
Such spots are invisible to the naked eye, and the
glow present is masked at the position where the
spots are at the final chromatogram.
Yellow
Distance of
component from
origin (X) in cm
4cm
Green
3.5cm
0.54
Light Green
2cm
0.30
REFERENCES
Light Yellow
0.5cm
0.07
0.62