Cide:No: 09482303 fret = ne q ‘JAWAHARLAL NEHRU TECHNOLOGIC. INIVERSITY HYDERABAD’ B.Tech IV Year Il Semester Examinations, May - 21> Animal Cell Science and Technology (Biotechnology) Answerainy Five Quédtiins All Questioiis Carry Eqiial Marks Mlustrate differences between primary and established cells List the equipment used in animal cell science a their uses, (748] Give an overview of differeni types of media. Yarious constituents for cell growth and differentiation, 115] ‘What are the various methods of cell growth measurements, x Piseuss the technique of measuring cell viabilty-and cyto toxicit [748] Elucidate various cell separation techniques, Waite a note on disageregation of tissue and primary cells. [7481 uss the applications of stem 05, 5. What are stem cells? How they are produced dis epereells? 6. Comment on the following a. Histotypic cultures, b. Skin grafts. ¢. Mixed cell cultures. Write notes on a. Micromanipulation, b. Transformetion. Synchrontzatic