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Microbiology Lec. 8
Viral Structure:
Viruses are composed basically of three
components; nucleic acid, protein coat, with or
without outer envelope.
1. Nucleic acid: is either DNA or RNA, but
never both. The nucleic acid can be single
stranded or double stranded & it contain all
the viral genome (genes).
2. Protein coat (Capsid): is composed of
repeated units of a same simple protein, each
unit is called capsomere, which carry the
antigenic determination of the virus & are
used when vaccines against viruses are
produced.
3. Outer envelope: is composed of
lipoproteins (phospholipids) & it has certain
tiny outer projections called spikes or
peplomeres.
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College of Dentistry
Microbiology Lec. 8
Notes:
The nucleic acid & the capsid are together
called nucleocapsid unit.
All viruses must have nucleic acid & capsid, but
the envelope can be present or not (not all
viruses have an outer envelope).
Viruses without envelope are called naked or
non-enveloped, while those that possess it
are called enveloped.
Capsid Symmetry:
Refers to the symmetry or arrangement of the
capsomeres around & in relation to the nucleic acid
molecule, it is of three types:
1. Icosahedral: The protein coat or capsid forms
a triangular tube of equilateral faces around the
nucleic acid. Most DNA viruses are of this type.
2. Helical: The capsid surrounds the nucleic acid
in a helical form (helix). Most RNA viruses are
of this type.
3. Complex: Where both of above symmetries
are found together in the same virus, e.g. pox
virus.
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College of Dentistry
Microbiology Lec. 8
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College of Dentistry
Microbiology Lec. 8
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College of Dentistry
Microbiology Lec. 8
Viral Replication:
It is a complex multi-step process, as viruses are
acellular; they don't have their own enzyme or
energy production system so they must use all the
enzymes of host cell as well as the ribosomes of the
host cell for protein synthesis. The steps of
replication are:
1. Adsorption.
2. Penetration.
3. Uncoating.
4. Transcription.
5. Synthesis of viral components & capsomeres.
6. Assembly.
7. Release.
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College of Dentistry
Microbiology Lec. 8
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College of Dentistry
Microbiology Lec. 8
Hepatitis B Virus:
v HBV is non-enveloped DNA virus with a unique
structure & mode of action.
v Hepadnaviridae family.
v The major proteins of HBV are the surface
antigen (HBs Ag) & core antigen (HBc Ag).
v Flowing infection, the virus replicates primarily
in hepatocytes with large amount of HBs Ag,
which then circulates in the blood.
v Acute infection may resolve with complete
elimination of the virus or may be followed by
chronic persistent infection which is associated
with a high risk of hepatic carcinoma (hepatoma).
Clinical Features:
HBV is transmitted by:
1. Sexual contact.
2. Parenteral inoculation of blood or blood
products through blood transfusion.
3. Parenteral drug abuse.
4. Tattooing or acupuncture.
5. From infected mothers to their infants during
birth.
The incubation period varies from 3-4 weeks to
nearly six months.
The majority of infection is asymptomatic.
10-20% of infected patients will have symptomatic
hepatitis.
Chronic infection is a major risk factor for the
development of hepatoma; the incubation period
may be up to 40 years.
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College of Dentistry
Microbiology Lec. 8
Diagnosis:
By detection of the excess HBs Ag present in serum
during both acute & chronic infection.
Screening of blood donors for HBs Ag has reduced
the incidence of HBV infection among recipient and
of blood & blood products.
Hepatitis A Virus:
v HAV is small Non-enveloped RNA virus.
v Picornavirus family.
v Transmitted primarily by the orofacial route.
v Following ingestion, the virus travel via the blood
stream to the liver.
v Replication in the liver result in (5-10) days of
viremia, & shedding of the virus in the stool for
(1-2) weeks.
v The infection resolves completely in all cases.
v Chronic or latent infection doesn't occur.
v The incubation period of HAV infection is 30 days
as an average (ranges from 10-50 days).
v Most infections, especially those in children, are
asymptomatic although evidence of chemical
hepatitis is usually found on lab test.
v Occurrence of the virus in blood: two weeks
before Jaundice to one week or less after
Jaundice, while HBV for months to years.
v Occurrence of the virus in stool: two weeks before
to two weeks after Jaundice, while HBV is absent
in stool.
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College of Dentistry
Microbiology Lec. 8
Routes of Transmission:
1. Sexual contact.
2. Through parenteral exposure to contaminated
blood or blood products.
3. From mother to child during delivery.
4. The presence of other sexually transmitted
disease (e.g. syphilis & gonorrhea) increases
the risk of sexual HIV transmission as much as
hundred fold.
Asymptomatic virus positive (+ve) individuals
can transmit the virus.
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College of Dentistry
Microbiology Lec. 8
Opportunistic Infection:
The main common causes of death in late stages
are:
1. Protozoa; Toxoplasma.
2. Fungi; Candida.
3. Bacteria; Mycobacterium.
4. Viruses; Cytomegalovirus (CMV).
Cancer:
AIDS patients develop cancer, like non-Hodgkin's
lymphoma (NHL) & Kaposi's sarcoma (KS).
Typed by:
Noor Al-Deen M. Al-Khanati
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