Beruflich Dokumente
Kultur Dokumente
Fredrick Murphy
October 13, 1976
Procedure
1) Place a droplet of fluid from cells on small screen
2) Let the fluid dry
3) Put specimen in electron microscope
4) Observe specimen
5) Clean area with bleach
Results
1) Sample was jammed with virus particles
2) Dried fluid looked like string
3) Inferred the virus was Marburg
Summary:
This experiment was performed on the blood from the Nun. The cell fluid was placed under the
electron microscope. Murphy saw that the cells were jammed with virus particles. This gave
the immediate assumption of Marburg virus. The lab area was now a hot zone. Murphy
decontaminated the area using Clorox bleach.
Patricia Webb
Date: October 13, 1976
Procedure
1) Open package with the Nuns blood
2) Put on gloves and used no other special precautions
3) Using cotton balls, dab up some blood
4) Squeeze cotton ball between gloved fingertips
5) Collect a few droplets of blood
6) Begin testing for viruses
7) Put blood droplets into flask of monkey cells
Results
1) Monkey cells became sick
2) Monkey cells began to die
3) Cells burst
4) Agent unknown
Summary:
This experiment was performed to test the Nuns blood. When the Nuns blood arrived the vials
were broken and the blood was black and like tar. The blood was tested for viruses, including
Marburg and other known viruses. During testing monkey cells burst and the live monkeys died.
The agent causing this does not match any known agents. This might be a new virus.
Eugene Johnson
September 2, 1987
Procedure
1) Put on surgical scrubs and rubber gloves
2) Carry box to Level 3
3) Open box to reveal sealed metal biohazard cylinders
4) Open biosafety level 4 cabinets
5) Place metal cylinder inside cabinets
6) Close and tighten cabinet door
7) Put hand into gloves of level 4 cabinet
8) Pick up cylinder while looking through level 4 cabinet window
9) Peel off tape on cylinder
10) Open cylinder
11) Remove 2 small vials of golden liquid
12) Took blood serum and dropped in flask of monkey cells
13) Inject virus strain into 3 different monkeys
14) Inject virus strain into guinea pigs
Results:
1) Monkey cells were bursting and dying.
2) Two monkeys died and the third went into borderline shock.
3) Guinea pigs were all killed - male guinea pig testicles swelled to the size of a golf ball
and turned purple.
Summary:
This lab experiment was performed to test Peter Cardinals blood. Peter died with the
combinations of extreme symptoms that could suggest an unidentified level 4 virus. His blood
was tested first in monkey cells. Then was injected directly into live monkeys and guinea pigs to
test the virus.