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Enzymes are proteins that speed up reactions like synthesis and digestion. Basically the
enzyme catalase lets the substrate (H2O2) so that it could be digested into H2O (Water) + O2
(Oxygen). Hydrogen peroxide is pretty toxic to to any living being organism, so we need to
digest it in order to prevent ourselves from getting extremely sick. Hydrogen peroxide is formed
whenever H2O and O2 is synthesized together with the catalase. When we digest, we break apart
the H2O2 and then separate the enzyme catalase from the H2O and the O2. In the lab, we had to
figure out the rate of reaction of the catalase as affected by the pH. So the prediction that my
team settled with was that if we increase the pH to 10, then the rate of reaction would increase.
LabQuest Mini
micropipette tips
Magnetic stirrer
Stirring bar
Thermometer
Hydrogen peroxide
On the blank lines, write the initials of the teammate who completed the task:
cable, and connect the Gas Pressure Sensor to CH 1 of the LabQuest Mini.
the flask.
stopper assembly to the Gas Pressure Sensor as shown in the image. The
valve connected to the stopper should stay closed during this investigation.
Complete all the steps below quickly to complete your test reaction.
13. __________Start data collection: click green Collect button on Logger Pro.
15. __________IMMEDIATELY tightly seal the flask by placing the stopper in and
HOLDING it in carefully.
NOTE: If the pressure exceeds 130 kPa, the pressure inside the flask will be
too great and the rubber stopper will likely pop off. HOLD DOWN the
18. __________Carefully remove the stopper from the flask to relieve the
pressure.
23. ___all_______Take off your safety gear and place it neatly away.
24. Observe the graph generated using LoggerPro software (example shown
below).
25. Hold down Control on the keyboard and press j to zoom in the graph.
26. Highlight the section of the graph where the slope is increasing, by clicking
27. Click the Analyze tab at the top of the page, and choose Linear Fit. A
statistics box will appear for your highlighted section of the graph.
28. Record the slope of the line, m, as the rate of catalase activity in kPa/s in
29. Click File, Save As, and save with the file name:
(Your Names) Sam, Anabel, Jenni, Jose Control Trial Logger Pro Data
30. Click File and choose Print Graph. In the drop down menu of printers, choose
Cute PDF Writer in the drop down list. This will export your graph as a
(Your Names) Sam, Anabel, Jenni, Jose Control Trial Data PDF
glenowitz@mesacharter.org
Data Analysis
The results from our data did not support the original hypothesis if we increase the pH to
10, then the rate of reaction would increase. The slope of the data before we changed the pH was
0.008590/ kPa/min. But after we changed the pH to 10, the slope didnt change much. It went
down to -0.01136 kPa/min, which means that the pH of 10 killed the enzyme, and it had no
reaction. These results actually make sense because enzymes only works under a specific range
of the pH depending on the type of enzyme it is. This enzyme worked very well with the pH of
6.5. But the pH of 10 was too high and the function the enzyme had originally was changed
because of the pH.
Conclusion
There may have also been some contributing factors as to why we got the results we had
the first time. The first time, it couldve been because the reaction was delayed and the catalase
was entered in too early. The spinning rate also couldve been a factor because the speed
couldve messed up the reaction. The next time we do this experiment, we could possibly wait to
make sure everything is actually working correctly before even moving further in the process.