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PE-6800

FULLY AUTO HEMATOLOGY ANALYZER


SERVICE MANUAL

Procan Electronics Inc.


5/F., 4thBldg.,NO.2,
Gaoxin C. 1st Ave.,
High-tech Industrial Park,
Nanshan,Shenzhen 518057, P.R. China
Tel: (+86) 755 26516667, 26952867
Fax: (+86) 755 26747910

Contents

CONTENTS
CHAPTER 1

INSTRUMENT INTRODUCTION.......................................................................... 1

1.1

STRUCTURE ..........................................................................................................................1

1.1.1

Host .......................................................................................................................................1

1.1.2

Accessory ..............................................................................................................................1

1.2

PURPOSE................................................................................................................................2

1.3

SPECIFICATIONS ..................................................................................................................3

1.4

STRUCTURE ..........................................................................................................................7

1.4.1

Front Panel.............................................................................................................................7

1.4.2

Rear panel..............................................................................................................................7

1.5

OPERATION ...........................................................................................................................9

1.5.1

Display Screen.......................................................................................................................9

1.5.2

Menu......................................................................................................................................9

1.6

DETECTION PRINCIPL ......................................................................................................12

1.6.1

Detection Principles of WBC, RBC, PLT............................................................................12

1.6.2

Principles of HGB Measurement.........................................................................................13

1.6.3

Volume Distribution of Blood Cell ......................................................................................13

1.6.4

Results and Calculation of Parameter Measurement ...........................................................14

CHAPTER 2

HARDWARE............................................................................................................ 16

2.1

CPU BOARD.........................................................................................................................16

2.1.1

Function...............................................................................................................................16

2.1.2

Interface...............................................................................................................................17

2.2

CCU BOARD ........................................................................................................................17

2.2.1

Function...............................................................................................................................17

2.2.2

Front Interface .....................................................................................................................17

2.3

TFT CABLE CONVERTING BOARD ..........................................................................................19

2.3.1

Function...............................................................................................................................19

2.3.2

Interface...............................................................................................................................19

2.4. LCD ADOPTER ..............................................................................................................................20


2.4.1. Function ....................................................................................................................................20
2.5 RECORDER BOARD ..................................................................................................................21
2.5.1 Function .....................................................................................................................................21
2.6 DOM CARD ...................................................................................................................................21
2.6.1 Function .....................................................................................................................................21
CHAPTER 3 TUBING...................................................................................................................... 22
3.1 TUBING GRAPH.........................................................................................................................22
3.2

STRUCTURE OF TUBING SYSTEM ..................................................................................23

3.2.1 Liquid road structure..................................................................................................................23


3.2.2

Tubing Sensor Unit..............................................................................................................23

3.2.3

HGB Unit.............................................................................................................................24

3.2.4

Dilute Unit ...........................................................................................................................24


T- 6807-30-02357

V1.0

Contents
3.2.5

Vacuum Unit ........................................................................................................................24

3.2.6

Auxiliary Unit......................................................................................................................24

CHAPTER 4 SYSTEM STRUCTURE............................................................................................ 25


4.1 DISASSEMBLE/REPLACE PARTS AND COMPONENTS.......................................................25
4.1.1 Disassemble/Replace the Sample Machine................................................................................25
4.1.2

Disassemble/Replace the Diluent Machine .........................................................................26

4.1.3

Disassemble/Replace the Vacuum Block.............................................................................26

4.1.4

Disassemble/Replace the Vacuum Pump Block...................................................................27

4.1.5

Disassemble/Replace the Piston Block................................................................................28

4.1.6

Disassemble/Replace Chamber............................................................................................29

CHAPTER 5 ADJUSTMENT ............................................................................................................. 31


5.1

GENERAL.............................................................................................................................31

5.2

ADJUST PROGRAM ............................................................................................................31

5.3

ADJUST PLACE ...................................................................................................................32

CHAPTER 6 MAINTENANCE ....................................................................................................... 33


6.1

ROUTINE MAINTENANCE................................................................................................33

6.1.1

Clean the Appearance of the Instrument..............................................................................33

6.1.2

Replace Fuse........................................................................................................................34

6.2

DAILY MAINTENANCE .....................................................................................................34

6.3

WEEKLY MAINTENANCE .................................................................................................34

6.4

MONTHLY MAINTENANCE ..............................................................................................35

6.5

YEARLY MAINTENANCE..................................................................................................35

6.6 THE MAINTENANCE BEFORE TRANSPORT OR FOR

INSTRUMENT THAT

WILL NOT BE USED FOR A LONG TIME. .....................................................................................35


CHAPTER 7

TROUBLESHOOTING .......................................................................................... 37

7.1 PROGRAM TEST ........................................................................................................................37


7.2

CHECKING ITEMS BEFORE MAINTENANCE ................................................................38

7.3

BACKGROUND CHECK.....................................................................................................38

CHAPTER 8 APPENDIX 1 LEAD LABEL....................................................................................... 41


CHAPTER 9 APPENDIX 2 DEBUG RECORD FORM .................................................................. 42
CHAPTER 10 APPENDIX 3 TROUBLESHOOTING ..................................................................... 45

II

T- 6807-30-02357

V1.0

Instrument Introduction

Chapter 1
1.1

INSTRUMENT INTRODUCTION

STRUCTURE

Host and accessory.

1.1.1

Host

Control the process of sample collection, dilution and analysis, including the following units:
Hematology analyzer

Former cover parts

RBC chamber

CCU board

Vacuum pump machine

Dilution machine

CPU Board

System software

Vacuum pump
block

Power supply

Dilution block

Sample machine

1.1.2

WBC chamber

TFT cable

Glass bulb

Adapter PCBA

Switch parts

Backboard parts

Transformer

Liquid tubing

Liquid Sensor

Accessory

Including the following accessories:


1. PS/2 mouse. PS/2 keyboard.
2. Diluent cap components, washing liquid cap components, hemolytic agent cap components,
1

Instrument Introduction
waste cap components;
3. PS232 Communication cable;
4. Waste barrels;
5. Printer (Optional ), Bar code scanner (Optional )

1.2

PURPOSE

It is used for detecting the parameters of RBC, WBC, HGB and differential counter.
The analyzer is used for the determination of the following 20 parameters and 3 histograms blood
specimens in Table 1-1.

Item

Abbreviation

Unit

White blood cell or leukocyte

WBC

109/L

Lymphocyte

LYM#

109/L

Mid-sized cell

MID#

109/L

Granulocyte

GRAN#

109/L

Lymphocyte percentage

LYM%

Mid-sized cell percentage

MID%

Granulocyte percentage

GRAN%

Red Blood Cell or erythrocyte

RBC

1012/L

Hemoglobin Concentration

HGB

g/L

Hematocrit

HCT

Mean Corpuscular(erythrocyte) Volume

MCV

fL

Mean Cell Hemoglobin

MCH

pg

Mean Cell Hemoglobin Concentration

MCHC

g/L

Red Blood Cell Distribution Width- Standard


Deviation

RDW-SD

fL

Red Blood Cell(erythrocyte) Distribution


Width Coefficient of Variation

RDW-CV

Platelet

PLT

109/L

Mean Platelet Volume

MPV

fL

Platelet Distribution Width

PDW

Plateletcrit

PCT

Plateletcrit-large Cell Ratio

P-LCR

White Blood Cell Histogram

WBC Histogram

Red Blood Cell Histogram

RBC Histogram

Platelet Histogram

PLT Histogram
Table 1-1

Instrument Introduction

1.3

SPECIFICATIONS

Standard Classification of the Instrument


According to anti-electrical shock hazard: Grade II, Pollution 2
According to the defence for deleterious liquid: General Device (Closing device of
non-defensive liquid)
According to the recommended disinfection/sterilization method of the manufactory:
Disinfectant device recommended by manufactory.
According to safety degree in condition that using gas mixture of flammable
anesthetic gas and air or gas mixture of oxygen and nitrous oxide: Do not use the
equipment in condition that using gas mixture of flammable anesthetic gas and air or
gas mixture of oxygen and nitrous oxide.
According to working status: Continuous Running Equipment.

Principles of Measurement
Blood Cells are counted and sized by Electrical Impedance Method. Hemoglobin is
determined by Colorimetry Method.

Parameters of Measurement

Basic parameters:
Item

Abbreviation

Unit

White blood cell

WBC

109/L

Red Blood Cell

RBC

1012/L

Hemoglobin Concentration

HGB

g/L

Platelet

PLT

109/L

Derived from Histograms:


Item

Abbreviation

Unit

Lymphocyte percentage

LYM%

Mid-sized cell percentage

MID%

Granulocyte percentage

GRAN%

Instrument Introduction
Mean Corpuscular(erythrocyte) Volume

MCV

fL

Red Blood Cell Distribution Width Standard

RDW-SD

fL

RDW-CV

Mean Platelet Volume

MPV

fL

Platelet Distribution Width

PDW

Plateletcrit-large Cell Ratio

P-LCR

Deviation
Red Blood Cell(erythrocyte) Distribution
Width Coefficient of Variation

Calculated Parameters:
Item

Abbreviation

Unit

Lymphocyte

LYM#

109/L

Mid-sized cell

MID#

109/L

Granulocyte

GRAN#

109/L

Hematocrit

HCT

Mean Cell Hemoglobin

MCH

pg

Mean Cell Hemoglobin Concentration

MCHC

g/L

Plateletcrit

PCT

Sampling Features:
Venous and Capillary Blood Mode:

Prediluted Mode:

WBC/HGB
RBC/PLT

1:44600

WBC/HGB

1:355

RBC/PLT

1:44500

Cell Counting Aperture Size:


WBC/RBC:

80m

Display
Liquid Crystal Display (LCD),resolution:640480

Language

1:300

Instrument Introduction
English

Indicator
20 Parameters and 3 Histograms Display
Alarm Indicator
Working Status (including Power Supply) Indicator Light
Alarm

Interface
One power input socket
One printer port
One VGA display port
Two RS232/C serial ports
Two USB interfaces
Two PS/2 interfaces

Recorder
Rapid Thermal Recorder

Recording width:

48mm

Paper width:

57.5mm

Cable adapter board, Liquid Sensor, Voltage

When the liquid reagent is in the sensor the voltage is 3.0V ~ 5.0V;
When the liquid reagent is not in the sensor the voltage is 1.5V

Pre-dilution draining capacity

Pre-dilution draining capacity range should be 8.00.4ml

Aperture voltage reference value

WBC Aperture voltage reference valuethe 5 times average W.H.V.S value of blank test
RBC Aperture voltage reference valuethe 5 times average R.H.V.S value of blank test.

Aperture voltage
Press T1

voltage range 9-20V

Press T2

voltage range less than 3V

Chamber cover and frame.

Take electrode cable from CCU board and use the two pens of multimeter (which
5

Instrument Introduction
have been set the resistance value) to measure the Chamber shield and substrate
Insulation. The value should be more than 20MWhen you finish test, return the
electrode cable back. The value should less than 1

Screen Display and the Ranges for Report Output Parameters


Parameter

Parameter Range

Parameter Range

WBC

0.0 - 99.9x109/L

GRAN#

0 - 99.9x109/L

RBC

0.00 - 9.99x1012/L

HCT

0.0 - 100.0%

HGB

00.0 - 300g/L

MCH

0.0 - 999.9pg

PLT

0 - 3000x109/L

MCHC

0.0 - 999.9g/L

MCV

0 - 250fL

RDW-SD

0.0 - 99.9 fL

LYM%

0 - 100%

RDW-CV

0.0 - 99.9%

MID%

0 - 100%

PDW

0.0 - 30.0%

GRAN%

0 - 100%

MPV

0.0 - 30.0fL

LYM#

0 - 99.9x109/L

PCT

0.0 - 9.99%

MID#

0 - 99.9x109/L

P-LCR

0.0-99.9%

Operating Ambient
Temperature:

15~35

Humidity:

10~90%

Atmospheric Pressure:

86.0kPa ~106.0kPa

Ambient Storage
Temperature:

-10~40

Humidity:

80%

Atmospheric Pressure:

Parameter

50.0kPa ~106.0kPa

Electric Specifications
Power Supply:

AC 100-240V,

501Hz,

The maximum input consumption is 150VA


Fuse:

AC 250V

2A

Instrument Introduction

Dimensions and Weight


Dimensions:

394mm381mm500mm

Weight:

23kg

1.4

STRUCTURE

1.4.1

Front Panel

Figure 1-1
1. Power Light

2. [START] Key

3. Sample Needle

4.Display Screen

5. Thermal Recorder

1.4.2

Rear panel

Instrument Introduction

5
4

6
2
7
3

18

17

16

15

14
13

12

1110

chart 1-2
1. Equipotentiality

2. PS/2 Keyboard Interface

3. PS/2 Mouse Interface

4. Printer Interface

5. USB Serial Interface

6.VGA Display Interface

7. RS-232 Serial Port 1

8.RS-232 Serial Port 2

9. Air Filter

10. Power Switch

11. Socket for Power Input

12. Fuse Holder

13. RINSE Connector

14. DILUENT Connector

15. WASTE Connector

16.WASTE Sensor Connector

17. LYSE Connector

18. Air Filter

Instrument Introduction

1.5

OPERATION

Designed according to human engineering and transferring information to users by software


windows and mouse, both are convenient for your operating.

1.5.1

Display Screen

The display screen is classified five sections:


SYSTEM TIME

INFORMATION

SECTION

SECTION

SHORTCUT KEY
SECTION

WINDOWS DISPLAY SECTION

AUXILIARY OPERATION SECTION


Figure 1-3
SYSTEM TIME SECTION
Display the current date and time of the system.
INFORMATION SECTION
Display prompt information of the system.

SHORTCUT KEY SECTION


To display the function interfaces of the system.

WINDOWS DISPLAY SECTION


Display various results.
AUXILIARY OPERATION SECTION
Display the information of user.

1.5.2

Menu

You can operate the instrument by menu. Press the right key of the mouse, directing to the
function menu of the current window. Move the mouse to the required menu bar, and then press
the left key of the mouse, the required function will be selected. Pressing the left key out of the
9

Instrument Introduction
menu area, the operation will be canceled.
The common functions are showed at the top right corner of the screen as shortcut key. Move the
mouse to the function you wanted.
The menu functions are as follows:
Sample Mode: Choose the sample mode among Venous Blood Mode, Capillary Blood Mode and
Prediluted Mode.
Diluent: Inject the diluent quantificationally by sample needle. This menu uses only in Prediluted
Mode.
Information: Enter the information window of the next sample, input and modify the
information.
Histogram: Enter the histogram adjustment window of the current sample. You can manually
adjust the histograms of the current sample.
Print: Output the data of the current sample to the appointed device.
Function: Enter the function menu.
Mute: Stop the alert.
Help: Access the system help window.
About: Display information of the instrument such as software and copyright etc.
Shutdown: Startup the shutdown program to rinse the measurement unit automatically. Shutoff
the power supply when the screen displays Please switch off.

Function Menu
Review: Enter the review window. You can review, inquire or modify the measurement data of the
former sample.
Calibration: Enter the calibration window to calibrate the instrument.
Quality Control: Enter the quality control window to carry out quality control operation.
Setting: Enter the setting menu.
Service: Enter the service menu.

Calibration Menu
Manual Calibration: Enter the manual calibration window to calibrate the instrument manually.
10

Instrument Introduction
Auto Calibration: Enter the auto calibration window to adjust the instrument automatically.

QC Menu
QC Edit: Enter the QC edit window to edit the QC parameters.
QC Run: Enter the QC run window to carry out the selected QC files.
QC Graphs: Enter the QC graphs window to browse the selected QC graphs.
QC Table: Enter the QC table window to browse the selected QC tables.

Setting Menu
Time: Enter the time setting window to set the time of the instrument.
Printout: Enter the printout window to set the functions such as print or report.
Config: Enter the config window to set the functions such as print, sleep, clean, and display etc.
Limits: Enter the limits window to modify the limits.
Department: Enter the department window to set the department and abbreviation.
Operator: Enter the operator window to set the department, abbreviation, department and
operator.

Service Menu
Flush: To get rid of the aperture clog.
Enhanced Flush: To get rid of hard aperture clog.
Cleaning: Run the normal rinse process to rinse the measurement unit.
Enhanced Cleaning: Rinse the measurement unit with RINSE.
Drain Chambers: Empty the liquid of the counter pool.
Park: Run the Park Program to rinse and empty the tubing.
Prime Diluent: Inject the Diluent to the appointed tubing.
Prime Lyse: Inject the Lyse to the appointed tubing.
Prime Rinse: Inject the Rinse to the appointed tubing.

Maintenance: Run the maintenance program to maintain the instrument.


Mechainc: Enter the Mechainc window to verify the functions of the instrument.
11

Instrument Introduction
Special: Enter the Special window to maintain the instrument.

1.6

DETECTION PRINCIPL

1.6.1

Detection Principles of WBC, RBC, PLT

The count principle of the instrument is based on the measurement of changes in electrical
resistance produced by a particle passing through an aperture sensor.
a) The sample blood is diluted in a conductive liquid. As blood cells are non-conductive, the
diluent is a good conductor. There are big differences between them.
b) When the diluent passes through the aperture sensor, electrodes are submerged in the liquid on
each side of the aperture to create continuous current.
c) When cells pass through the aperture, the resistance between the electrodes increases as the
cell volume increasing, as is shown in figure 1-4.According to the Ohm Formulary: U=RI
(U=Voltage I=Current R=Resistance).If

I is a constant, U increases as the cell volume

increasing.
d) Passing through the magnification circuit, the voltage signal will be magnified and the noise
will be filtered, then you will gain the analytical results, as shown in figure 1-4.
e) One count pool and the detection circuit count the WBC. Another count pool and the detection
circuit count the RBC and PLT. The microprocessor of the instrument calculates and analyzes
the cells (WBC, RBC, PLT), and then gives out the histograms.
f)

PLT count adopts advanced liquid, electronics and software system. It settles the repetitive
count of the cells on the side of the aperture count area.

12

Instrument Introduction

Figure 1-4

1.6.2

Principles of HGB Measurement

Adding lyse in the blood, the red blood cell will rapidly be broken down and release hemoglobin.
Hemoglobin and lyse form a new mixture, which can absorb the wavelength of 540nm.Measure
the absorbency. Through the comparison of the absorbency between the pure diluent and the
samplethe concentration of the sample hemoglobin is calculated.

1.6.3

Volume Distribution of Blood Cell

When different types of cells pass through the aperture sensor, there will be different electrical
pulse height. Because of the evident difference of the cells sizes, the instrument is able to
differentiate the white blood cell, red blood cell and platelet by its pre-set program.
The volume distributions are as follows:
WBC

1201000fL

RBC

8298fL

PLT

235fL

The leucocytes disposed by lyse can be divided into three types, according to their volumes:
lymphocyte (LYM), mid-sized cell (MID), Granulocyte (GRAN).
LYM
MID

3590 fL
90160 fL
13

Instrument Introduction
GRAN

1.6.4

160450 fL

Results and Calculation of Parameter Measurement

The parameters of the sample blood test can be described in three ways:
a) Measured directly, such as WBC, RBC, PLT, HGB.
b) Derived from histograms, such as LYM%, MID%, GRAN%, MCV, RDW-SD,
RDW-CV, MPV, PDW, P-LCR.
c) Calculated, such as LYM#, MID#, GRAN#, HCT, MCH, MCHC, PCT.
The derivation of the formularies as follows:
z

MCV derives from histograms and the instrument collecting and classifying the
erythrocytes according to its volume. It is determined by measuring the average volume
of individual erythrocytes, and the unit is fL.

RDW represents the volume distribution of the erythrocyte populations, derived from
the RBC histogram. It can be expressed by the coefficient variation of the erythrocyte
volume as RDW-CV, and the unit is %.It can also be expressed in standard deviation of
the erythrocyte volume as RDW-SD, the unit is fL.

MPV is the average volume of individual platelets, derived from the PLT histogram. It
represents the mean volume of the PLT populations and can be expressed in fL.

PDW derives from the PLT histogram. It represents the geometry standard deviation (10
GSD) of the volume of the PLT populations.

P-LCR derives from the PLT histogram. It represents the ratio of the larger PLT and the
unit is %.

14

HCT(%)= RBCMCV/10

MCH(pg)= 10HGB/RBC

MCHC(g/L)= 100HGB/HCT

PCT(%)= PLTMPV/10

LYM% = 100 x AL /AL+AM+AG

MID% = 100 x AM /AL+AM+AG

GRAN% = 100 x AG/AL+AM+AG

LYM# = LYM% x WBC/100

Instrument Introduction
z

MID# = MID% x WBC/100

GRAN# = GRN x WBC/100

AL: Number of cells in LYM area;


AM: Number of cells between lymphocyte and granulocyte area;
AG: Number of cells in GRAN area.

15

Hardware

Chapter 2

HARDWARE

The instrument has the following hardware


CPU Board, CCU Board, cable adapter board, liquid tubing optical conpler optocoupler adapter
board, motor optocoupler adapter board, DOM card, LCD adapter board, transformer, recorder
Board, switching power supply and inverter.

2.1

CPU BOARD

2.1.1

Function

With CPU as the core, the computer system also includes some peripheral equipments, such as
DRAM, SRAM, besides there are some interfaces, such as USB interface, COM2 interface,
COM1 interface, LPT printer interface, power supply interface, mouse/keyboard interface, display
screen TFT cable interface, peripheral display interface, DOM interface. As the chart 2-1.
The basic components of the computer system are CPU, DRAM, SRAM. They also supplied the
basic running environment for software.

16

Hardware

2.1.2

Interface

1 COM1interface
2 COM2 interface
3 USB interface

4 Mouse interface
3

5 Keyboard interface
6 Display

TFT

cable

interface
7 VGA external display
interface
4
5

8 DOM card interface


9 LPT printer interface

6
7

Chart 2-1

2.2

CCU BOARD

2.2.1

Function

The CCU board consists of six modules:


Digital Circuit
Drive Circuit
Power Circuit

2.2.2

Analog/Digital converted

Analog Circuit

Circuit
HGB Measurement Circuit

Front Interface

17

Hardware
1

5
Chart 2-2

18

Hardware
10

11

Chart 2-3
1. Cpu Interface

2.

Power Supply Interface

3 .Step Motor C Sensor And Optocoupler Interface

4.

Valve Interface

5. Liquid sensor interface

6.

HGB Cable Interface

7. Temperature Sensor Interface.

8. 110v Ignition Voltage Input Interface

9. Probe Electrode Cable Interface

10.

Indicator Led Interface

11 Recorder 20pin Flat Cable Interface

2.3

TFT Cable Converting Board

2.3.1

Function

The function of TFT Cable Converting Board is to connect all blocks and CCU board, and to
adjust the sensitivity of liquid sensor through adjusting the trimmer on TFT Cable Converting
Board.

2.3.2

Interface

19

Hardware

Liquid sensor jack

Adjustable potentiometer

Valve jack

Motor and optocoupler jack

Chart 2-4

2.4. LCD adopter


2.4.1. Function
The main function of LCD is to connect screen and CPU with cable and TFT through FPC soft
cable. It is used to transmit signal from CPU to display screen. Display as following: chart 2-5.
FPC soft cable socket can contact with screen directly

TFT cable jack (to contact with MTB board ) directly.


Chart 2-5

20

Hardware

2.5 RECORDER BOARD


2.5.1 Function
The main functions of the recorder board are to receive and deal with the printing data, and then
transmit the data to thermal printer. At the same time, the recorder board drives the motor to print
the received data. It is as the chart

Power
supply
interface

Record cable interface

Chart 2-6

2.6 DOM card


2.6.1 Function
DOM card is used for deposition. It should make sure the DOM card is in correct way before
insert it. As the following chart:

Drawing tablets

DOM jack

Drawing tablets

Chart 2-7

21

Tubing

Chapter 3

TUBING

3.1 TUBING GRAPH

Chart 3-1

22

Tubing

3.2

STRUCTURE OF TUBING SYSTEM

3.2.1 Liquid road structure


1 Vacuum pump machine.
2 Lyse sensor.

3 Vacuum pump block.

1
7

2
4

4 Dilution sensor.
5 Waste liquid sensor.
6 Dilution block

7 Sample machine.
8 Glass bulb
9 Cleaner sensor.

3.2.2

Chart 3-2

Tubing Sensor Unit

The instrument includes four tubing sensor: diluent tubing sensor, rinse tubing sensor, lyse tubing
sensor, waste liquid sensor and reagent temperature sensor.
The tubing sensor, rinse tubing sensor, lyse tubing sensor are sued to check if the reagent enters
into the tubing system. If there is no reagent in the tube. The instrument will give alarm of no
diluent or no cleaner or no lyse.
Waste liquid sensor is used to test if the waste liquid is full. If yes, there instrument will give alarm
of waste liquid full.
reagent temperature sensor is used to check the reagent temperature in the tube. If the temperature
is too low the instrument will give alarm of temperature too low.

23

Tubing

3.2.3

HGB Unit

This part is used to measure the hemoglobin concentration.

3.2.4

Dilute Unit

This part consists of sample sucker structure and dilution structure. Sample sucker structure is
used to aspirate sample, add it into the probe structure and clean the sample needle. Dilution
structure is used to provide power to suck sample.

3.2.5

Vacuum Unit

This part is made up of vacuum pump and vacuum decelerating structure.


The vacuum decelerating structure is working under the system software and is used to provide
power in the condition of electrifying. Vacuum pump is driven by vacuum decelerating structure
and produce negative pressure. So that to provide working power to all this tube system such as
waste emissions.

3.2.6

Auxiliary Unit

This part mainly refers to the tubing hose, connecting tube and electromagnetically operated
valve.

24

System Structure

Chapter 4

SYSTEM STRUCTURE

4.1 DISASSEMBLE/REPLACE PARTS AND COMPONENTS


4.1.1 Disassemble/Replace the Sample Machine
Block

slider

Chart 4-1
25

System Structure

It is necessary to replace the sample needle wipe block, seal washer and synchronous driving belt
of the sample machine periodically. The replacing cycle is one year.

4.1.2

Disassemble/Replace the Diluent Machine

4.1.3

Disassemble/Replace the Vacuum Block

26

System Structure

4.1.4

Disassemble/Replace the Vacuum Pump Block

It is necessary to replace the seal washer of the vacuum pump periodically. The replacing cycle is
one year.

27

System Structure

4.1.5

Disassemble/Replace the Piston Block

It is necessary to replace the seal washer of the piston block periodically. The replacing cycle is
one year.

28

System Structure

4.1.6

Disassemble/Replace Chamber

Chart 4-6

29

System Structure

Chart 4-7

30

Adjustment

Chapter 5 ADJUSTMENT
This part introduces how to adjust the gain of the passage.

5.1

GENERAL

5.1.1

Replace the chamber

2800 probe components: RBC, WBC gain, HGB gain.


5.1.2

Replace the Aperture

Aperture: WBC gain, RBC gain


5.1.3

Replace CCU board

Need to adjust the gain of the WBC, RBC/PLT, HGB.

5.2 ADJUST PROGRAM


5.2.1 Adjust calibration parameter.
In main operation window of the instrument, click the right key of the mouse, pops up the menu.
Move the cursor to Calibration/Manual Calibration. Adjust the calibration factors to 100%.
Click the right key of the mouse, and pops up the menu, then move the cursor to
Service/Maintenance, enter the password 6666, and click F12key.
Click the right key of the mouse, pops up the menu, then move the cursor to
Calibration/Factory Manual Calibration. Adjust the calibration factors to 100%.
Use the calibration of Procan recommended. In main operation window, measure the calibration.
According to the measured histograms to adjust the gain of the passage. Adjust the gain of passage
WBC, RBC according to the Multi-Parameter Assayed Hematology Control Expected Results
Sheet.
In main operation window of the instrument, click the right key of the mouse, pops up the menu.
then move the cursor to Service/Test, enter the password 6800,click HGB Background/T
key to adjust the potentiometer VR5,setting the voltage as 4.310.02V.

31

Appendix 2 Debug record form

5.3 ADJUST PLACE

12

1. Adjustment of WBC Gain


3. Adjustment of HGB Gain

32

2. Adjustment of RBC Gain

Maintenance

Chapter 6

MAINTENANCE

As other precision instrument, only careful daily service and periodic maintenance can the
instrument have a good working status, and can we get the reliable measurement results and have
less malfunctions. This chapter introduces some preventive methods for service and maintenance.
If you want to know more relative information, please contact the customer service department of
Procan.
According to the requirements for maintenance of the instrument during the using procedures, we
divide the preventive service and maintenance into the following types: daily, weekly, monthly,
yearly and maintenance according to actual need.
Warning:
If the hospital or agency dont perform a satisfying plan to maintain the instrument, there
will be abnormal malfunction.

6.1

ROUTINE MAINTENANCE

6.1.1

Clean the Appearance of the Instrument

Wipe the appearance of the instrument with neutral detergent or distilled water.

Caution:
Avoid using corrosive acids, alkali, and volatile organic solvent such as: acetone, aether,
chloroforms to wipe the appearance of instrument. Only can use the neutral detergent.

Caution:
Avoid wiping the inner of the instrument.

33

Maintenance

6.1.2

Replace Fuse

The fuse is installed in the fuse-box on the side of power switch. Open the box to replace the fuse
expediently.
Appointed specification fuse: AC 250V 2A

Warning:
Only can use the appointed specification fuse.

6.2

DAILY MAINTENANCE

It can be divided into two types: run and shutdown.


Run
The instrument has installed daily maintenance procedure. On running,
execute auto cleaning

it can

procedure according to the quantity of the sample to keep the

instrument in good working status. Set the auto cleaning procedure according to the
section 6.3. The general setup principles are:
Working time > 8 hours, auto-cleaning time=8 hours;
4 hours<Working time<8 hours, auto-cleaning time=4 hours;
Working time< 4 hours, auto-cleaning time=2 hours
The auto-cleaning time will decrease 1 hour year by year.
Shutdown
The instrument should be operated by auto maintenance before shut off the instrument.
You only need to clean the workbench and wipe the appearance of instrument when the
power-off.

6.3

WEEKLY MAINTENANCE
This instrument has installed weekly maintenance procedure. Act as follows:
a) Prepare E-Z cleanser and probe cleanser.
b) In the main operation window, press the right key of the mouse, pops up the menu.

34

Maintenance
In the menu area, select Service/Maintenance. Operate as the screen shows.
c) Turn off the power supply.
d) Wipe the appearance of instrument as section 6.1.1.

6.4

MONTHLY MAINTENANCE
It is necessary to clean the dust of the instrument monthly.
Operate as follows:
a)

Turn off the power supply, and pull out the power line.

b)

Hold up the air filter cover with screwdriver.

Take off the cover and the net. Clean them with neutral detergent brush and put them in the
shade.
c)

Airing naturally .Brush away the dust clinging on the air filter grid at the rear panel of
instrument.

d)

Put the air filter grid on the air filter net carefully. Planish them.

Notice:
Only can use the neutral detergent cleaning the air filter. Avoid airing it with heat.

6.5

YEARLY MAINTENANCE

Its necessary to perform preventive maintenance once every year. Because of the
highly-requirements for yearly maintenance, the maintenance should be performed by the
authorized engineer of Procan. Please contact the customer service department of Procan before
yearly maintenance.

6.6

THE

MAINTENANCE

BEFORE

TRANSPORT

OR

FOR

INSTRUMENT THAT WILL NOT BE USED FOR A LONG TIME.


If the instrument is not going to be used in 2 weeks or longer, or need to pack and transport,
performing the following procedures:
a)

In the main operation window, press the right key of the mouse, choose Service/Park

35

Maintenance
in the menu.
b)

Operate as screen shows.

c)

Rotate the bottle covers of rest reagents and store them as the reagent operation
introduction. User should perform efficient action to prevent the material from
deteriorating, mis-eating and misusing.

d)

Plug the stopples which were pulled out at the first installation into the corresponding
tube connector.

e)

Pull out diluent connection tube, rinse connection tube, lyse connection tube and waste
connection tube and clean them with distilled water. Dry them in shady place, then
packing them into plastics.

36

f)

Pull out the power wire, packing it in plastics after cleaning with neutral detergent.

g)

Put the instrument and parts packed in plastics into packing cases.

Troubleshooting

Chapter 7

TROUBLESHOOTING

7.1 PROGRAM TEST


Test instrument through the following programme:

Make sure the


ambient
temperature
humidity
atmospheric
pressure are in

Start

instruments,

observation
self-test

Start up abnormal

equipment

is

normal,

moving parts is running

NG

Moving parts run


abnormal

smoothly, and liquid road


system without leakage and
other

abnormalities,

Liquid
road
system leakage

apparatus with alarm or not

Make sure if the


reagent is enough,
original reagent
and not expired

OK

Blank

Blank data does not


meet the technical
requirements

Test

Observe the test

Make sure the


voltage is correct
to our instrument

Mainten
ance

all

data and data gaps


is in compliance
with

The amount of data

technical

requirements

Maintenan

NG

does

or

not

technical

not

meet

the

requirement

--- High

OK
Make sure the
reagent
is
connected correct
and reliable

Sample test
The test result is
steady or not (CV
value is accord with

NG

Sample tests do
not
meet
technical

Maintenance

technical

Make sure if any


part is connected
correctly
and
reliable.

requirement

OK
END

Chart 7-1

37

Troubleshooting

7.2

CHECKING ITEMS BEFORE MAINTENANCE

Items required for checking before maintenance:


Ambient Temperature:

1535

Temperature of the reagent: 1530


Humidity:
Atmospheric Pressure:

1090%
86.0kPa106.0kPa

If the temperature is below 15,the activity of the reagent will reduce. It will weaken the capacity
of dissolving the blood. In a certain time, it can not break down the red blood cell membranes into
2fl cellular debris. At lower temperature conditions, the red blood cell debris will gather together.
It will affect the measurement of leukocyte, resulting in the count value of WBC increasing .It will
also affect the measurement of HGB, resulting in the count value of HGB increasing.
Checking for Sample Blood
Sample Collection: Observe whether the operator uses correct method to collect the blood
(Operating as PE-6800 FULLY AUTO HEMATOLOGY ANALYZER OPERATION MANUAL
/Chapter 3/Section 3.5 Preparation For Sample Collection), and whether the sample is stored in
the container filling with EDTA.K2O.H2O anticoagulant.
Sample Storage: After the sample blood is well-mixed, test on the instrument promptly. As time
goes by, the volume and density of the cells will change. The environment and the patient
determine the varying rate of the volume and density. If the sample is placed a long time at room
temperature, the volume of RBC will increase, and the MCV, RDW, PDW will be affected, and the
PLT will easily gather together.
Affection of WBC Classification: To get the exact measuring results, the venous blood sample
measurement must be completed within 4 hours. If the sample blood is placed a long time at room
temperature, the total number of WBC may change little, but the classification is inaccurate.

7.3

BACKGROUND CHECK

Background Check: This item can ensure the measurement and the calculated data wont be
affected by reagent empty. If the background value exceeds the permissive area, the measured and
calculated data will be inaccurate.
The permissive range of the background test results is
38

Troubleshooting

Parameter

Background Range

Unit

WBC

0.2109/L

109/L

RBC

0.021012/L

1012/L

HGB

1g/L

g/L

HCT

0.5

PLT

10109/L

109/L

Background Test Method: When power-on, suggested to run background test, you can also
execute background test as you need.
The operation steps are as follows:
a)

In the blood cell analyzer window, press Info button, and pops up the information
edit window. Then click the item textbox, change the ID as 999999999.then press
OK, return to blood cell analyzer window.

b)

Background count in venous or capillary mode, press the START key below the
sample needle directly for background count. In prediluted mode, the sample needle
aspirates some non-contaminated diluent from the chamber for background count.
The diluent pick-up method as follows(Analyzing the sample in prediluted mode, it
is also necessary to dilute sample with the diluent which is prior picked-up in this
method):

In main operation area, press the right key of the mouse, pops up the menu. In menu operation
area, select Sample Mode/ Prediluted to set the sample mode as prediluted mode.
In main operation area, press the right key of the mouse, pops up the menu. In the menu operation
area, select Diluent. Place a clean empty chamber or tube under the sample needle, press
START key to dispense the diluent into the chamber. Press the START key once, the diluent
is dispensed into the chamber or tube once (The quantity can be used for background count in the
second prediluted mode).
Press Exit button to return to the main operation area.
Affected Factors of Background:
1

The reagent is dirty.

The chamber is dirty.

39

Troubleshooting
3

Electronic interference affects measurement and the calculated circuit, for example:
Interference created by voltage fluctuation, peripheral equipment such as centrifugal
machine, CT machine, magnetic resonance device, etc.

40

Tubing Contamination

Lead label

Chapter 8 Appendix 1 Lead label


No.

Stick place

Label name

note

Motor A

MA

Sample structure monitor in the


kickstand place

Motor B

MB

Sample structure monitor in


horizontal axis fixed plate

Motor C

MC

Dilution monitor

Motor D

MD

Vacuum decelerating monitor

Optocoupler A

OSA

Sample structure switch in the


kickstand place

Optocoupler B

OSB

Sample structure switch in


horizontal axis fixed plate

Optocoupler C

OSC

Dilution switch

Optocoupler D

OSD

Vacuum decelerating switch

Dilute sensor

DLS

10

Lyse sensor

LYS

11

Cleaner sensor

CLS

12

Waste liquid
sensor

WST

13

switch

STA

14

MTB board

MTB

15

valve

V1.V2V16

The lead is according to the No.


of valve

41

Debug record form

Chapter 9 Appendix 2 Debug record form


Name : Host debugging technics
Serial No. of product
Item

No: F-6807-30-02301
Temperature

Edition
Humidity

Requirement

Result

OK

Not ok.

Refer to PE-6807 the requirement of host production


requirement

OK
Not ok.

Display screen

Should display full screen and every label should


clear and no color cast

OK
Not ok.

Mouse interface

Check the connection between jack and mouse is


connected well and can work in good way.

OK

Not ok.

Keyboard interface

Check the connection between jack and keyboard is


connected well and can work in good way.

OK
Not ok.

Indicator light

It will light when standby and shine when suck


sample.

OK
Not ok.

Reagent alarm

With words and sound alarm

OK

Not ok.

With right direction and without noice cacophony

OK
Not ok.

Date and time

Local date and time

OK
Not ok.

valve

With correct place and there is sound when connected


with electricity.

OK

Not ok.

OK
Not ok.
V

appearance

According to the requirement of standard products

fitting

Backboard fan

Data circuit

It will display ok when we check it.

HGB black voltage

Adjust to 4.31V

Burning function

Chamber have sound of Chichi. The indicator light


will be on.

OK
Not ok.

Motor
associated
parts

Smooth operation, no abnormal sound, non-blocking


phenomenon
And the status of the test results should be "OK"

OK
Not ok.

OK

Not ok.

and
moving

Sampling needle tip


position
perpendicular to the
direction of origin

Adjust the position with the sampling needle position


tool

Negative pressure of
vacuum chamber

79.21 KPa

Vacuum
leak rate

0.2 KPa /2Min

chamber

5.0V voltage test

42

5.000.5V

KPa
KPa /2Mins
V

Note

Debug record form


Reagent temperature
test

Aperture voltage test

Draining the amount


of pre-diluted mode
Test the voltage of
liquid sensor

Air Mixer
The
ability
of
chamber
shield
(cover) and the
substrate insulation

Temperature of thermometer should be 5 the


same as temperature inside of chamber
Click
Aperture
voltage

Less than3V

ml

Test the voltage of liquid sensor is 3.0V5.0V with


reagent

Test the voltage of liquid sensor is 1.5V without


reagent

There is air bubble come out from bottom of cup.

Resistance should be more than 20M

OK
Not ok.

OK
Not ok.

Lymphocyte peak

fL

RBC peak

fL

WBC

0.2109/L

0109/L

RBC

0.021012/L

01012/L

HGB

1g/L

0g/L

HCT

0.5

Oversee the time of


blank counting

OK
Not ok

0
9

1010 /L

0109/L

12S+1S

Set the Reference


voltage value of
pinAperture

Channel data volume

920V

8.00.4ml

PLT

Add liquid cleaning


kits

up

Click
down
Aperture
voltage

Channel gain

Take count in blank

OK
Not ok

WBC

RBC

The liquid should flow cling to the needle uniformly


and there is no departure of needle and no share
phenomenon

The
average of
blank test
result
The
average
value of 5
times
counting
time
Every time
of blank
test
is
ok.

OK
Not ok.

WBC

200KB

OK

RBC

100KB

Not ok.

Any
blank
43

one
test

Debug record form

Carrying
contamination test

Instrument
calibration

PLT

300KB

WBC

0.5

RBC

0.5%

OK

HGB

0.5%

Not ok.

PLT
Venous
and
Periphera
l blood
calibratio
n
paramete
r

1.0%
WBC
RBC
HGB
MCV

OK
Not ok.

OK
Not ok.

Pre
dilute
calibration
parameter

Accuracy testCV

Test accuracy

result

PLT
WBC
RBC
HGB
MCV
PLT

WBC accuracy test

3.0%

RBC accuracy test

3.0%

HGB accuracy test

3.0%

MCV accuracy test

1.0%

PLT accuracy test

7.0%

WBC accuracy
error range

6%

RBC accuracy
error range

5%

HGB accuracy
error range

4%

MCV accuracy
error range

5%

PLT accuracy
error range

14%

OK
Not
ok.

Venous blood

Predilution

model

model

OK
Not
ok.

Printing characters, graphics should be correct, clear. The


Record and alarm

functioning of institutions should be smooth. There is no abnormal


sound, no paper block but with alarm function phenomenon, there

OK
Not ok.

are alarm.
Pinter and net
function
WAperture
estimation.

44

Printing characters, graphics should be correct, clear.Meanwhile


the PC can accept complete and correct data and graphic.
OK

Not ok.

OK
Not ok.

Troubleshooting

Chapter 10 Appendix 3 TROUBLESHOOTING


Error Message
Fuse burn-out

Possible Reason

Troubleshooting

Fluctuating input AC voltage

Use stabilized voltage power

Power supply board is short circuit

Replace new power supply board

There are bubbles in chamber

Exclude the bubbles

The reagent is inadequate

Re-prime the reagent

The setup of count time is too long

Reset the count time

The aperture is broken

Renew the aperture

The count passage leaks

Replace the leakage count tubing

The back cover and body of chamber lacks

Replace the the back cover of sample


cover

Abnormal

The reagent is contaminated

Renew the reagent

background test

Electronic interference

Connect the ground wire

Tubing contamination

Clean the tubing

There are large-cells or debris in the


sample

Remove the debris from the sample

The setup of count time is too short

Reset the count time

Aperture clogs

Clean the aperture

Inadequate diluent

Check for diluent and piston block

MTB is broken

Replace MTB board

Incorrect connection of the signal line

Re-connect the signal line

Liquid crystal display screen is aging

Replace the display screen

The WBC chamber is dirty ,

Dip in the chamber ,or adjust the gain


of HGB

The battery is not get enough light

Replace battery or WBC probe


components

LBD Is Not Light Enough

Replace the LBD or WBC probe


components

The CPU board or the keyboard is broken

Replace the CPU board or the


keyboard

Bubbles

Clogs

Unclear display

Abnormal

HGB

background
voltage

Some keys of the


keyboard
is
irresponsive

supply

45

Troubleshooting

Liquid leaks

Abnormal vacuum chamber pressure

Replace vacuum chamber

The waste tubing is obstructed

Dredge the waste tubing

The sample is placed a too long time

Measure
minutes

The anticoagulant is superfluous

Reduce the quantity of anticoagulant.

Reagent is used up

Renew the diluent

No reagent,

Fluid hose seal in bad way

Rinse(diluent/lyse)

Check the connection and get rid of


liquid lack

The piston block leaks

Replace the piston block or seal ring

empty alarm

The rinse(diluent/lyse) sensor is broken ,or


the connector is broken off

Replace the rinse(diluent/lyse) sensor

The chamber is dirty

Clean the chamber

The count time is longer than setup time

Reset the count time

One sensor of the MTB board is broken

Replace MTB board

The reference voltage of Aperture is much


lower than the actual test values

Reset the
Aperture

There is no result

There are problems on CCU board

Replace or repair CCU board

of

The electrode cable is broken

Replace or repair probe components

The instrument is effected by high


electromagnetic machine. Such as the
instrument is not connected well with
ground

Get rid of the electromagnetic


machine.

The liquid road is dirty

Clean the liquid road and


operate maintenance programme.

Abnormal
mid-sized
percentage

cell

WBC/ RBC clogs

RBC,

WBC

the

sample

reference

within

voltage

30

of

measurement

The result of RBC,


WBC is high

The sample is stared too long time

46

Get new samples.