Bacteriology

1st Quiz
1. Give at least three (3) culture media utilized for a mans urethral discharge.
Thayer-Martin
Martin-Lewis
Modified Thayer-Martin
New York City Agar
CAP
2. Minimum amount of bacterial colonies per mL for a patient to be diagnosed with UTI.
100,000/mL (urine is the only specimen that needs to be counted)
3. Lowest concentration of antibiotic that inhibits growth of a test organism.
Minimum Inhibitory Concentration
4. 70 colonies of S.aureuswere found in SBAP after inoculation of 0.001 mL of urine.
How many CFU/mL of urine should be reported?
70,000 CFU/mL
5. Give at least two (2) selective media for gram positive organisms.
Columbia Colistin Nalidixic Agar/Phenyl Ethyl Alcohol Agar
6. A penicillin-resistant Neisseria gonorrhoeaeproduces what substance?
B-lactamase
7. ONPG test classifies organisms as lactose fermenters by testing for the presence of
what substance?
B-galactosidase
8. Give at least three (3) differential culture media for lactose and non-lactose fermenters
MAC, EMB, HEA
9. This bacteria gives off characteristic swarming in the culture media
Proteus
10. This bacteria demonstrates colonies that have a sea-gull appearance in the culture
media
C. jejuni
11. This is a modified CAP that contains vancomycin, colistin, anisomycin and
trimethoprim
Martin-Lewis
12. When performing a Kovac indole test, the substrate must contain what substance?
Tryptophan
13. What are the four (4) samples that can be cultured to diagnose Typhoid fever?
Blood, BM aspirate, Urine, Stool
14. What is the best and most sensitive sample that can be cultured to diagnose typhoid
fever?
BM aspirate
15. What is the best culture medium for culture of Bordetella pertussis?
Regan-Lowe Medium
16. What is the best medium for culture of Francisella tularensis?
Cystine Glucose Agar
17. What is the specific medium of choice when processing throat swabs for group A
Streptococcus culture?
Sheep Blood Agar

18. The Wayson stain is a stain used to identify what specific bacteria?
Y. pestis
19. These organisms are bipolar bacilli that resemble safety pins.
Y. pestis
20. It is considered the most pleomorphic bacteria in microscopy
C. diphteriae
21. This organism demonstrates a satellite phenomenon around colonies of
Staphylococcus
H. influenzae
22. Characteristic color demonstrated by Staphylococcus aureus in MAC
Golden Yellow
23. Characteristic color demonstrated by Non-Staphylococcus aureus species in MAC
Colorless/pink
24. X and V factors are utilized to identify what species of bacteria?
Haemophilus spp
25. The most common bacteria isolated from wound aspirates
Pseudomonas aeruginosa (expose to increase amount of O2)
26. Most common cause of meningitis in immune compromised people
L. monocytogenes
27. Most common cause of meningitis in infants and newborns
S. agalactiae
28. Most common cause of meningitis in children from 1 to 5 years old
H. influenzae
29. The porphyrin test was devised to detect strains of Haemophilus influenzae capable of
producing what substance?
X-factor
30. This bacteria has a characteristic bleach-like odor in its colonies and demonstrates
colonies that pit in SBAP
Eikenella corrodens
31. Which of the following specimens can be accepted for anaerobic culture? (vaginal
discharge, eye discharge, pleural fluid, brain abscess, urine)
Brain Abscess/Pleural Fluid
32. Differentiate an enrichment medium from an enriched medium
Enrichment liquid, broth, capacity to give off nutrient and inhibit
contaminants
Enriched solid, plate, capacity to give nutrient
33. Enrichment medium specific for Vibrio organisms
APN
34. Selenite F is an enrichment medium specific for what bacteria?
Salmonella and Shigella
35. What is the main reason why SPS is a good anticoagulant for blood cultures?
Inhibit action of neutrophils and complements in blood.
36. Most appropriate specimen source and medium match. Which of the following is the
most appropriate specimen source and media combination?
Endocervical- CAP, Martin-lewis
Sputum- SBAP, Thayer-martin

CSF- Columbia CAN, MAC

Urine- SBAP, CAP, Columbia CAN
Endocervical (CAP & ML)
37. Meaning of CIN in CIN agar
Cefsulodin-Irgasan-Novobiocin
38. CIN agar is best used for selection and differentiation of what bacteria?
Yersinia enterocolitica and Aeromonas
39. Dicks test is a skin susceptibility test for what bacteria?
S. pyogenes
40. Shicks test is a skin susceptibility test for what bacteria?
C. diphteriae
41. McBride medium is a medium used for what species of bacteria?
L. monocytogenes
42. Antons test is a test used for the identification of what bacteria?
L. monocytogenes
43. What are specifically used in the performance of Antons test of bacterial
identification?
Conjunctiva of rabbit/ guinea pig
44. What is the composition of a Modified Thayer-Martin Agar?
Vancomycin, Nestatine
45. Give the four (4) modifications of CAP culture media used for Neisseria species
identification
Thayer-Martin, Martin-Lewis, Modified Thayer-Martin, New York City Agar
46. Carbohydrate fermentation test of Neisseria species utilizes what agar media?
CTA- cysteine trypticase agar