Beruflich Dokumente
Kultur Dokumente
Review
1 Introduction
Intensive aquaculture system produces substantially
higher yields than do semi-intensive systems. However,
intensification results in stressful environmental changes
which can produce various kinds of problems in aquatic
organisms, principally diseases of diverse aetiology
(Angulo, 2000). The most common control of the diseases was concentrated on the use of antibiotics. However,
the utility of antibiotics as a preventive measure has been
questioned by acquisition of genes that result in antibiotics resistance and horizontal spread of plasmids from fish
pathogenic to humans. Plasmids carrying resistance de-
Source
Turbot larvae
Used on
Method of administration
Reference
Scophthalmus maximus
Oncorhynchus mykiss
Penaeus monodon
Oreochromis niloticus
Rainbow trout (Oncorhynchus mykiss)
Rainbow trout
Addition to diet
Commercial products
(Cernivet)
Anguilla anguilla
Addition to diet
Gatesoupe, 1994
Irianto and Austin,
2002
Rengipat et al., 1998
Naik et al., 1999
Gram et al., 1999
2 Modes of Action
The mode of action of probiotic strains is likely to be
multifactorial and, from existing evidence, appears to be
strain specific (Touhy et al., 2003). Enhancement of
colonization resistance and/or direct inhibitory effects
against pathogens is likely to be important in situations in
which probiotics have reduced the incidence and duration
of diseases. Probiotic strains have inhibited pathogenic
bacteria both in vitro and in vivo through several different
mechanisms. This includes protection creating a hostile
environment for pathogens by the production of inhibitory compounds such as bacteriocins, siderophores, lysozymes, proteases, hydrogen peroxide, formation of
ammonia and diacetyl, alteration of pH values by the
production of organic acid (Verschuere et al., 2000),
competition for essential nutrients and adhesion sites
(Vine et al., 2004), supply of essential nutrients and enzymes resulting in enhanced nutrition of the cultured
animal (Wang et al., 2000), direct uptake of dissolved
organic material mediated by the bacteria (Dalmin et al.,
2001), modulating interactions with the environment and
the development of beneficial immune responses (Gullian
et al., 2004).
Competitive exclusion is a phenomenon whereby an
established microbiota prevents or reduces the colonization of bacterial pathogen by competition for the attachment sites on the mucosa, competition for nutrients, and
production of inhibitory substances by the microbiota
which prevents replication and destroys the challenging
bacteria. Several mechanisms are involved in the adhesion of microorganisms to intestinal epithelial cells such
as passive forces, electrostatic interactions, hydrophobic,
steric forces, lipoteichoic acids and specific structures.
These properties could confer a competitive advantage
important for bacterial maintenance in the animals gastrointestinal tract. Since mucosal surfaces are colonized
by a large, complex and dynamic collection of microorganisms, it is extremely important for intestinal epithelial
cells and the intestinal mucosal immune system to discriminate between pathogen and non-pathogen bacteria
and to generate an appropriate response, maintaining a
state of homeostasis with microbiota or initiating inflammatory responses against pathogens (Shi and Walker,
2004).
Several studies on the beneficial effects of probiotics in
the digestive process of the fish have been reported.
Ring et al. (1995) suggested that species of Agrobacterium, Pseudomonas, Brevibacterium, Microbacterium and
Staphylococcus isolated from the intestinal tract of alpine
trout (Salvelinus alpinus L.) contributed to the nutritional
process by the production of lipases that increase the absorption of lipids. Sugita et al. (1997) reported bacteria
that constitute the intestinal microbiota (especially Aeromonas sp. and Pseudomonas sp.) from freshwater fish in
Japan (carp, channel catfish, tilapia and japanese ell),
77
3 Selection of Probiotics
A common way to screen the candidate probiotics is to
perform in vitro antagonism test, since bacterial antagonism is a common phenomenon in nature. Therefore, several investigators have used this phenomenon to select
candidates by in vitro tests, in which pathogens are exposed to the candidate probiotics or their extracellular
products in liquid and/or solid medium. Nevertheless, it
has been reported that in vitro activity using liquid and/or
solid medium can not be used to predict a possible effect
in vivo. For example, in vitro antagonism of Pseudomo
nas fluorescents (strain AH2) against A. salmonicida did
not confer protection in Atlantic salmon against furunculosis, but it conferred protection against vibriosis (Gram
et al., 2001). Therefore, it is necessary to know the origin
of the stock (preferably to use strains isolated from the
host in study) and its security (non-pathogenic).
Some bacteria used as candidate probiotics have antiviral effects. Although the exact mechanism by which
these bacteria do this is not known, laboratory tests indicate that the inactivation of viruses can occur by chemical
and biological substances, such as extracts from marine
algae and extracellular agents of bacteria. It has been reported that strains of Pseudomonas sp., Vibrios sp.,
Aeromonas sp., and groups of coryneforms isolated from
salmonid hatcheries, showed antiviral activity against
78
5 Pioneering Studies
The first report seems to be attributed to Yasuda and
Taga (1980), who suggested that bacteria would be found
to be useful not only as food but also as biological controllers of fish diseases and activators of nutrient regeneration. Later, Nogami and Maeda (1992) isolated a strain
PM-4 from the rearing water of larval Penaeus monodon,
with good survival and molting rates. The bacterium was
identified as Thalassobacter utilis (Nogami et al., 1997).
This biocontrol treatment increased the survival of the
larvae and repressed the growth of Vibrio anguillarium
and the fungus Haliphthoros sp. (Nogami et al., 1997).
Since 1995, there have been increasing successes with
the use of probiotics in the Ecuadorian shrimp industry,
especially in controlling the high incidence of larval diseases. A V. alginolyticus strain isolated from seawater on
6 Conclusions
Relevant information shows that probiotics can reduce
the incidence and duration of diseases. The use of probiotics can increase the survival rate and control the high
incidence of larval diseases. It is essential to understand
the mechanisms of action in order to define selection criteria for potential probiotics. Using probiotics in aquaculture has been in large part a result of historical and
empirical use and not based on scientific criteria.
References
Angulo, F., 2000. Antimicrobial agents in aquaculture: potencial
impact on public health. APUA Newsletter, 18: 1-5.
Balczar, J. L., I. de Blas, I. Ruiz-Zarzuela, D. Vendrell, J. L.
Muzquiz, et al., 2006. The roll of probiotics in aquaculture.
Veter. Microbiol., 114: 173-186.
Balczar, J. L., D. Vendrell, I. Ruiz-Zarzuela, and J. L. Muzquiz,
2004. Probiotics: a tool for the future of fish and shellfish
health management. J. Aqua. Trop., 19: 239-242.
Balczar, J. L., 2002. Uso de probioticos en acaucultura: aspectos generales. In: Memorias del Primer Congreso Iberoamericano Virtual de Acuicultura. de Blas, I., eds., Zaragoza,
Espaa, 877-881.
Chang, C. I., and W. Y. Liu, 2002. An evaluation of two probioticbacterial strains, Enterococcus faecium SF68 and Bacillus
toyoi, for reducing edwardsiellosis in cultured European eel,
Anguilla anguilla L. J. Fish Dis., 25: 311-315.
Dalmin, G., K. Kathiresan, and A. Purushothaman, 2001. Effect
of probiotics on bacterial population and helth status of
shrimp in culture pond ecosystem. Indian J. Exp. Bio., 39:
939-942.
Garriques, D., and G. Arevalo, 1995. An evaluation of the production and use of a live bacterial isolate to manipulate the
microbial flora in the commercial production of Penaeus
vannamei postlarvae in Ecuador. In: Swimming Through
Troubled Water. Proceedings of the Special Session on Shrimp
Farming. Browdy, C. L., and J. S. Hopkins, eds., San Diego,
CA, USA, 53-59.
Gatesoupe, F. J., 1994. Lactic acid bacteria increase the resistance of turbot larvae, Scophthalmus maximus, against
pathogenic. Vibrio. Aquat. Liv. Res., 7: 277-282.
Gomez-Gil, B., A. Roque, and J. F. Turnbull, 2000. The use and
selection of probiotic bacteria for use in the culture of larval
79