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ACCUCARE

ALBUMIN
B.C.G. colorimetric test
ORDER INFORMATION
Cat. No.
ALB 100
ALB 200

LINEARITY

Kit Size
2x50 ML
2x100 ML

The method is linear to a concentration of 8.0 g/dl

Quantitative determination of albumin in serum/plasma


IVD
CLINICAL SIGNIFICANCE
Observations of serum albumin level is useful as an aid in diagnosing
disease states of the liver and kidneys. Moderate to large changes in the
concentration of albumin have significant effects on the relative amounts
of the bound and free concentrations of the ligands it carries: because free
ligands are those that interact with tissue receptor sites and that can be
excreted, albumin levels have important influences on the metabolism of
endogenous substances such as calcium, bilirubin, and fatty acids and on
the effects of drugs and hormones. Hypoalbuminemia is very common in
many illnesses and results in most instances from one or more of the
following factors: 1) impaired synthesis, 2) increased catabolism, 3)
reduced absorption of amino acids, 4) altered distribution which may
sequester large amounts of albumin in an extravascular compartment, 5)
protein loss by way of urine or feces.

AUTOMATED PARAMETERS
Wavelength
Cuvette
Reaction Temperature
Measurement
Reaction
Reaction Direction
Sample / Reagent Ratio
Incubation
Low normal
High Normal
Linearity
ASSAY PROCEDURE
PIPETTE INTO TEST TUBES
BLANK

STD

SAMPLE

5 l

Sample
PRINCIPLE
The measurement of serum albumin is based on its quantitative binding to
the indicator 3, 3, 5, 5 - tetrabromo-m-cresol sulphophthalein
(bromocresol green, BCG). The albumin-BCG complex absorbs maximally
at 578 nm.
REAGENT COMPOSITION
Reagent I
:
Albumin standard :

BCG reagent
4 g/dl (store at 2-8C)

620 nm
1 cm light path
Room Temperature
Against Reagent Bl
End Point
Increasing
1 : 200
5 minutes
1.8 g/dl
5.1 g/dl
8.0 g/dl

Standard

5 l

Reagent

1000 l

1000 l

1000 l

Mix well, and wait for 5 mins at Room Temperature. Measure the
absorbance of the Sample (Ac) and Standard (As) against the reagent
blank.
CALCULATION
Ac/As x Conc. Std. = g/dl Albumin

SAFETY PRECAUTIONS AND WARNINGS


1.
2.
3.
4.

For in vitro diagnostic use only.


DO NOT pipette by mouth. Avoid contact with skin and eyes. If spilt,
thoroughly, wash affected areas with water. For further information,
consult the Albumin Reagent Material Safety Data Sheet.
Reagent contains Sodium Azide as a preservative. This may react
with copper or lead plumbing to form explosive metal azides. Upon
disposal, flush with large amounts of water to prevent azide build up.
Do not use the reagent after the expiration date printed on the kit.

The method is linear upto a concentration of 8.0 g/dl.


If the concentration exceeds this value, the sample should be diluted 1:1
with 0.9% saline solution and reassayed. Multiply the result by 2.
QUALITY CONTROL
Accutestrol N - H
REFERENCE INTERVAL
Serum, plasma : 1.8 - 5.1 g/dl

SAMPLE COLLECTION AND PRESERVATION


Serum or plasma.
REAGENT PREPARATION AND STORAGE

BIBLIOGRAPHY
E.M. Gindler and J. O. Westgard Clin. Chem., (1973), 6,4.
J.O. Westgard, M.A. Poquette, Clin. Chem., (1973) 19, 647.

The reagent is ready to use.


REAGENT STABILITY
Up to expiry date when stored at room temperature.

LAB-CARE DIAGNOSTICS (INDIA) PVT. LTD.


C1 Type, Shed No. 3225, Chemical Zone, GIDC Sarigam,
SARIGAM - 396 155 (Dist. Valsad). INDIA
Tel : 91-22-2554 2109 / 2554 1558 Fax : 2554 3541
Email : accucare@labcarediagnostics.com
W ebsite : www.labcarediagnostics.com